MC <p>415e</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Sucrase (Suc)</td><td align="right">6.5</td><td align="right">51.0</td></tr><tr><td>Aprotinin (Apr)</td><td align="right">9.2</td><td align="right">6.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 5.5</b>?</p> A. Both Suc and Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Suc and Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Suc will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Suc will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>399f</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Lipase (Lip)</td><td align="right">4.7</td><td align="right">40.0</td></tr><tr><td>Aldolase (Aldo)</td><td align="right">8.5</td><td align="right">47.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both Lip and Aldo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Lip and Aldo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Lip will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Aldo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Lip will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Aldo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>00b7</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Ferritin (Fer)</td><td align="right">5.5</td><td align="right">19.8</td></tr><tr><td>Myelin Basic Protein (MBP)</td><td align="right">9.5</td><td align="right">18.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both Fer and MBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Fer and MBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Fer will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> MBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Fer will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> MBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>d296</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Avidin (Av)</td><td align="right">10.5</td><td align="right">16.9</td></tr><tr><td>Estrogen Binding Protein (EBP)</td><td align="right">4.9</td><td align="right">115.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both Av and EBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Av and EBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Av will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> EBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Av will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> EBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>500d</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Sucrase (Suc)</td><td align="right">6.5</td><td align="right">51.0</td></tr><tr><td>Leghemoglobin (Leg)</td><td align="right">4.7</td><td align="right">16.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both Suc and Leg will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Suc and Leg will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Suc will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Leg will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Suc will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Leg will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>18d0</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Catalase (Cat)</td><td align="right">6.7</td><td align="right">65.5</td></tr><tr><td>Cytochrome c (Cyt)</td><td align="right">10.2</td><td align="right">13.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.5</b>?</p> A. Both Cat and Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Cat and Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Cat will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Cat will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>037a</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>DNA Ligase (Lig)</td><td align="right">6.0</td><td align="right">60.0</td></tr><tr><td>Horseradish peroxidase (HP)</td><td align="right">9.0</td><td align="right">34.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both Lig and HP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Lig and HP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Lig will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> HP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Lig will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> HP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>aa76</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Hemoglobin (Hem)</td><td align="right">7.1</td><td align="right">16.7</td></tr><tr><td>Aprotinin (Apr)</td><td align="right">9.2</td><td align="right">6.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.0</b>?</p> A. Both Hem and Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Hem and Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Hem will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Hem will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>95e7</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Lipase (Lip)</td><td align="right">4.7</td><td align="right">40.0</td></tr><tr><td>Prostate-Specific Antigen (PSA)</td><td align="right">6.4</td><td align="right">30.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both Lip and PSA will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Lip and PSA will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Lip will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> PSA will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Lip will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> PSA will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>6ab0</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Horseradish peroxidase (HP)</td><td align="right">9.0</td><td align="right">34.0</td></tr><tr><td>Pepsin (Pep)</td><td align="right">1.0</td><td align="right">34.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 10.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 10.0</b>?</p> A. Both HP and Pep will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both HP and Pep will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. HP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Pep will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. HP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Pep will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>1cff</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Fibrinogen (Fib)</td><td align="right">5.8</td><td align="right">63.5</td></tr><tr><td>Elastase II (Ela)</td><td align="right">8.5</td><td align="right">26.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both Fib and Ela will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Fib and Ela will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Fib will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Ela will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Fib will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Ela will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>ecc9</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>SARS-CoV-2 Spike Protein (CoV)</td><td align="right">6.2</td><td align="right">149.9</td></tr><tr><td>Horseradish peroxidase (HP)</td><td align="right">9.0</td><td align="right">34.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both CoV and HP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both CoV and HP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. CoV will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> HP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. CoV will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> HP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>3a6e</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Cytochrome c (Cyt)</td><td align="right">10.2</td><td align="right">13.0</td></tr><tr><td>Estrogen Binding Protein (EBP)</td><td align="right">4.9</td><td align="right">115.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both Cyt and EBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Cyt and EBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> EBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> EBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>4c75</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Estrogen Binding Protein (EBP)</td><td align="right">4.9</td><td align="right">115.0</td></tr><tr><td>Histone (His)</td><td align="right">10.8</td><td align="right">15.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both EBP and His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both EBP and His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. EBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. EBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>9a4b</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Serum Albumin (Alb)</td><td align="right">4.9</td><td align="right">66.2</td></tr><tr><td>Phospholypase (Pho)</td><td align="right">10.5</td><td align="right">19.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both Alb and Pho will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Alb and Pho will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Pho will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Pho will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>04e4</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>γ-Globulin (Glob)</td><td align="right">6.6</td><td align="right">57.0</td></tr><tr><td>Ribonuclease A (RibA)</td><td align="right">9.3</td><td align="right">13.7</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both Glob and RibA will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Glob and RibA will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Glob will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> RibA will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Glob will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> RibA will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>04f4</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Estrogen Binding Protein (EBP)</td><td align="right">4.9</td><td align="right">115.0</td></tr><tr><td>Succinate Ligase (SL)</td><td align="right">6.6</td><td align="right">20.9</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both EBP and SL will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both EBP and SL will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. EBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> SL will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. EBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> SL will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>55a9</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Histone (His)</td><td align="right">10.8</td><td align="right">15.0</td></tr><tr><td>Elastase II (Ela)</td><td align="right">8.5</td><td align="right">26.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both His and Ela will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both His and Ela will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Ela will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Ela will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>2921</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Horseradish peroxidase (HP)</td><td align="right">9.0</td><td align="right">34.0</td></tr><tr><td>α-Amylase (Amy)</td><td align="right">5.9</td><td align="right">61.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both HP and Amy will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both HP and Amy will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. HP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Amy will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. HP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Amy will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>75dc</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Salmine (Sal)</td><td align="right">12.1</td><td align="right">3.0</td></tr><tr><td>Luciferase (Luc)</td><td align="right">5.7</td><td align="right">50.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 9.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 9.0</b>?</p> A. Both Sal and Luc will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Sal and Luc will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Sal will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Luc will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Sal will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Luc will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>f504</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Myoglobin (Myo)</td><td align="right">7.0</td><td align="right">18.0</td></tr><tr><td>Ovalbumin (Ova)</td><td align="right">4.6</td><td align="right">45.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both Myo and Ova will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Myo and Ova will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Myo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Ova will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Myo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Ova will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>bba1</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Actin (Act)</td><td align="right">5.3</td><td align="right">43.0</td></tr><tr><td>Immunoglobulin (IgG)</td><td align="right">7.3</td><td align="right">145.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.5</b>?</p> A. Both Act and IgG will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Act and IgG will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Act will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> IgG will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Act will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> IgG will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>d2fd</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Enolase (Eno)</td><td align="right">8.4</td><td align="right">42.5</td></tr><tr><td>Fibrinogen (Fib)</td><td align="right">5.8</td><td align="right">63.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both Eno and Fib will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Eno and Fib will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Fib will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Fib will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>4fd8</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Salmine (Sal)</td><td align="right">12.1</td><td align="right">3.0</td></tr><tr><td>Actin (Act)</td><td align="right">5.3</td><td align="right">43.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.5</b>?</p> A. Both Sal and Act will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Sal and Act will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Sal will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Act will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Sal will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Act will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>6370</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Fumerase (Fum)</td><td align="right">7.6</td><td align="right">48.5</td></tr><tr><td>Salmine (Sal)</td><td align="right">12.1</td><td align="right">3.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 10.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 10.0</b>?</p> A. Both Fum and Sal will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Fum and Sal will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Fum will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Sal will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Fum will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Sal will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>a09d</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Aldolase (Aldo)</td><td align="right">8.5</td><td align="right">47.5</td></tr><tr><td>Trypsin (Tryp)</td><td align="right">10.8</td><td align="right">23.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 12.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 12.0</b>?</p> A. Both Aldo and Tryp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Aldo and Tryp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Aldo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Tryp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Aldo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Tryp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>0033</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Transferrin (Tran)</td><td align="right">5.9</td><td align="right">80.0</td></tr><tr><td>Ribonuclease A (RibA)</td><td align="right">9.3</td><td align="right">13.7</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 10.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 10.5</b>?</p> A. Both Tran and RibA will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Tran and RibA will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Tran will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> RibA will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Tran will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> RibA will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>ad4d</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Chymotrypsin (Chy)</td><td align="right">8.4</td><td align="right">25.0</td></tr><tr><td>Casein (Cas)</td><td align="right">4.9</td><td align="right">24.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.5</b>?</p> A. Both Chy and Cas will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Chy and Cas will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Chy will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Cas will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Chy will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Cas will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>2236</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>DNA Ligase (Lig)</td><td align="right">6.0</td><td align="right">60.0</td></tr><tr><td>Aprotinin (Apr)</td><td align="right">9.2</td><td align="right">6.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both Lig and Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Lig and Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Lig will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Lig will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>ba91</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Myoglobin (Myo)</td><td align="right">7.0</td><td align="right">18.0</td></tr><tr><td>Serine Protease (Ser)</td><td align="right">4.1</td><td align="right">22.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both Myo and Ser will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Myo and Ser will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Myo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Ser will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Myo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Ser will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>bc38</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Epidermal Growth Factor (EGF)</td><td align="right">4.5</td><td align="right">40.0</td></tr><tr><td>Phospholypase (Pho)</td><td align="right">10.5</td><td align="right">19.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 11.5</b>?</p> A. Both EGF and Pho will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both EGF and Pho will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. EGF will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Pho will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. EGF will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Pho will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>32ab</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Trypsin (Tryp)</td><td align="right">10.8</td><td align="right">23.5</td></tr><tr><td>Casein (Cas)</td><td align="right">4.9</td><td align="right">24.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both Tryp and Cas will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Tryp and Cas will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Tryp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Cas will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Tryp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Cas will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>5c9f</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Epidermal Growth Factor (EGF)</td><td align="right">4.5</td><td align="right">40.0</td></tr><tr><td>Cytochrome c (Cyt)</td><td align="right">10.2</td><td align="right">13.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both EGF and Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both EGF and Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. EGF will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. EGF will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>2fb3</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Collagen (Col)</td><td align="right">6.6</td><td align="right">134.0</td></tr><tr><td>Avidin (Av)</td><td align="right">10.5</td><td align="right">16.9</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 11.5</b>?</p> A. Both Col and Av will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Col and Av will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Col will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Av will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Col will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Av will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>8843</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Histone (His)</td><td align="right">10.8</td><td align="right">15.0</td></tr><tr><td>Insulin (Ins)</td><td align="right">5.4</td><td align="right">5.8</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both His and Ins will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both His and Ins will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Ins will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Ins will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>f940</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Fumerase (Fum)</td><td align="right">7.6</td><td align="right">48.5</td></tr><tr><td>Neuraminidase (Neu)</td><td align="right">5.2</td><td align="right">50.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.5</b>?</p> A. Both Fum and Neu will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Fum and Neu will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Fum will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Neu will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Fum will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Neu will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>bef5</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Immunoglobulin (IgG)</td><td align="right">7.3</td><td align="right">145.0</td></tr><tr><td>Xylosidase (Xyl)</td><td align="right">5.0</td><td align="right">100.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.5</b>?</p> A. Both IgG and Xyl will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both IgG and Xyl will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. IgG will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Xyl will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. IgG will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Xyl will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>7cfe</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Ferritin (Fer)</td><td align="right">5.5</td><td align="right">19.8</td></tr><tr><td>G3P Dehydrogenase (GDH)</td><td align="right">8.3</td><td align="right">36.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both Fer and GDH will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Fer and GDH will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Fer will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> GDH will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Fer will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> GDH will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>6bd4</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>G3P Dehydrogenase (GDH)</td><td align="right">8.3</td><td align="right">36.0</td></tr><tr><td>Lysozyme (Lys)</td><td align="right">11.0</td><td align="right">14.4</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both GDH and Lys will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both GDH and Lys will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. GDH will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Lys will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. GDH will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Lys will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>a28b</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Serum Albumin (Alb)</td><td align="right">4.9</td><td align="right">66.2</td></tr><tr><td>Lysozyme (Lys)</td><td align="right">11.0</td><td align="right">14.4</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both Alb and Lys will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Alb and Lys will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Lys will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Lys will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>f01a</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Fibrinogen (Fib)</td><td align="right">5.8</td><td align="right">63.5</td></tr><tr><td>Enolase (Eno)</td><td align="right">8.4</td><td align="right">42.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 9.5</b>?</p> A. Both Fib and Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Fib and Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Fib will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Fib will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>6678</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Insulin (Ins)</td><td align="right">5.4</td><td align="right">5.8</td></tr><tr><td>Trypsin (Tryp)</td><td align="right">10.8</td><td align="right">23.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both Ins and Tryp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Ins and Tryp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Ins will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Tryp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Ins will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Tryp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>a9d3</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Succinate Ligase (SL)</td><td align="right">6.6</td><td align="right">20.9</td></tr><tr><td>Lysozyme (Lys)</td><td align="right">11.0</td><td align="right">14.4</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 9.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 9.0</b>?</p> A. Both SL and Lys will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both SL and Lys will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. SL will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Lys will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. SL will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Lys will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>c1ac</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Luciferase (Luc)</td><td align="right">5.7</td><td align="right">50.0</td></tr><tr><td>Myelin Basic Protein (MBP)</td><td align="right">9.5</td><td align="right">18.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both Luc and MBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Luc and MBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Luc will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> MBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Luc will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> MBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>fbbe</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Collagen (Col)</td><td align="right">6.6</td><td align="right">134.0</td></tr><tr><td>Trypsin (Tryp)</td><td align="right">10.8</td><td align="right">23.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 5.5</b>?</p> A. Both Col and Tryp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Col and Tryp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Col will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Tryp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Col will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Tryp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>cf46</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Horseradish peroxidase (HP)</td><td align="right">9.0</td><td align="right">34.0</td></tr><tr><td>Serum Albumin (Alb)</td><td align="right">4.9</td><td align="right">66.2</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both HP and Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both HP and Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. HP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. HP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>6901</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Luciferase (Luc)</td><td align="right">5.7</td><td align="right">50.0</td></tr><tr><td>Pepsin (Pep)</td><td align="right">1.0</td><td align="right">34.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both Luc and Pep will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Luc and Pep will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Luc will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Pep will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Luc will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Pep will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>330c</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Ovalbumin (Ova)</td><td align="right">4.6</td><td align="right">45.0</td></tr><tr><td>Enolase (Eno)</td><td align="right">8.4</td><td align="right">42.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.5</b>?</p> A. Both Ova and Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Ova and Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Ova will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Ova will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>76a1</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Xylosidase (Xyl)</td><td align="right">5.0</td><td align="right">100.0</td></tr><tr><td>Hemoglobin (Hem)</td><td align="right">7.1</td><td align="right">16.7</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.5</b>?</p> A. Both Xyl and Hem will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Xyl and Hem will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Xyl will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Hem will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Xyl will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Hem will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>5757</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Elastase II (Ela)</td><td align="right">8.5</td><td align="right">26.5</td></tr><tr><td>Serum Albumin (Alb)</td><td align="right">4.9</td><td align="right">66.2</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.5</b>?</p> A. Both Ela and Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Ela and Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Ela will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Ela will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>54ec</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Enolase (Eno)</td><td align="right">8.4</td><td align="right">42.5</td></tr><tr><td>Agglutinin (Agg)</td><td align="right">4.8</td><td align="right">22.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.5</b>?</p> A. Both Eno and Agg will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Eno and Agg will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Agg will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Agg will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>6be5</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Green Fluorescent Protein (GFP)</td><td align="right">6.2</td><td align="right">28.0</td></tr><tr><td>Cytochrome c (Cyt)</td><td align="right">10.2</td><td align="right">13.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both GFP and Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both GFP and Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. GFP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. GFP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>5322</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Ferritin (Fer)</td><td align="right">5.5</td><td align="right">19.8</td></tr><tr><td>G3P Dehydrogenase (GDH)</td><td align="right">8.3</td><td align="right">36.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 9.5</b>?</p> A. Both Fer and GDH will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Fer and GDH will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Fer will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> GDH will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Fer will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> GDH will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>2916</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>β-Galactosidase (Gal)</td><td align="right">4.6</td><td align="right">175.0</td></tr><tr><td>Elastase II (Ela)</td><td align="right">8.5</td><td align="right">26.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.5</b>?</p> A. Both Gal and Ela will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Gal and Ela will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Gal will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Ela will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Gal will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Ela will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>d992</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Aprotinin (Apr)</td><td align="right">9.2</td><td align="right">6.5</td></tr><tr><td>Myoglobin (Myo)</td><td align="right">7.0</td><td align="right">18.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.0</b>?</p> A. Both Apr and Myo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Apr and Myo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Myo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Myo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>9765</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Succinate Ligase (SL)</td><td align="right">6.6</td><td align="right">20.9</td></tr><tr><td>Lactalbumin (Lac)</td><td align="right">4.3</td><td align="right">13.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.0</b>?</p> A. Both SL and Lac will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both SL and Lac will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. SL will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Lac will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. SL will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Lac will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>c0ed</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Elastase II (Ela)</td><td align="right">8.5</td><td align="right">26.5</td></tr><tr><td>α-Amylase (Amy)</td><td align="right">5.9</td><td align="right">61.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both Ela and Amy will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Ela and Amy will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Ela will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Amy will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Ela will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Amy will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>7135</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Serum Albumin (Alb)</td><td align="right">4.9</td><td align="right">66.2</td></tr><tr><td>Enolase (Eno)</td><td align="right">8.4</td><td align="right">42.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 9.5</b>?</p> A. Both Alb and Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Alb and Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>03cf</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Heat Shock Protein (HSP)</td><td align="right">5.5</td><td align="right">70.1</td></tr><tr><td>Ribonuclease A (RibA)</td><td align="right">9.3</td><td align="right">13.7</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both HSP and RibA will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both HSP and RibA will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. HSP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> RibA will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. HSP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> RibA will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>bf85</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Actin (Act)</td><td align="right">5.3</td><td align="right">43.0</td></tr><tr><td>Avidin (Av)</td><td align="right">10.5</td><td align="right">16.9</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 11.5</b>?</p> A. Both Act and Av will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Act and Av will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Act will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Av will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Act will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Av will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>2727</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Myelin Basic Protein (MBP)</td><td align="right">9.5</td><td align="right">18.0</td></tr><tr><td>Fibrinogen (Fib)</td><td align="right">5.8</td><td align="right">63.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 4.5</b>?</p> A. Both MBP and Fib will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both MBP and Fib will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. MBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Fib will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. MBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Fib will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>c8f2</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Ferritin (Fer)</td><td align="right">5.5</td><td align="right">19.8</td></tr><tr><td>Histone (His)</td><td align="right">10.8</td><td align="right">15.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both Fer and His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Fer and His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Fer will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Fer will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>abcf</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Cytochrome c (Cyt)</td><td align="right">10.2</td><td align="right">13.0</td></tr><tr><td>Enolase (Eno)</td><td align="right">8.4</td><td align="right">42.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 11.5</b>?</p> A. Both Cyt and Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Cyt and Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>b48e</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Actin (Act)</td><td align="right">5.3</td><td align="right">43.0</td></tr><tr><td>Avidin (Av)</td><td align="right">10.5</td><td align="right">16.9</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both Act and Av will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Act and Av will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Act will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Av will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Act will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Av will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>d268</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Hemoglobin (Hem)</td><td align="right">7.1</td><td align="right">16.7</td></tr><tr><td>Serine Protease (Ser)</td><td align="right">4.1</td><td align="right">22.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 5.5</b>?</p> A. Both Hem and Ser will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Hem and Ser will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Hem will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Ser will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Hem will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Ser will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>d2aa</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Horseradish peroxidase (HP)</td><td align="right">9.0</td><td align="right">34.0</td></tr><tr><td>Ferritin (Fer)</td><td align="right">5.5</td><td align="right">19.8</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both HP and Fer will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both HP and Fer will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. HP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Fer will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. HP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Fer will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>0eb4</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Aldehyde Dehydrogenase (Alde)</td><td align="right">5.3</td><td align="right">53.0</td></tr><tr><td>Cytochrome c (Cyt)</td><td align="right">10.2</td><td align="right">13.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both Alde and Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Alde and Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Alde will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Alde will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>ea15</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Fumerase (Fum)</td><td align="right">7.6</td><td align="right">48.5</td></tr><tr><td>Epidermal Growth Factor (EGF)</td><td align="right">4.5</td><td align="right">40.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.0</b>?</p> A. Both Fum and EGF will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Fum and EGF will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Fum will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> EGF will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Fum will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> EGF will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>6bca</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Ferritin (Fer)</td><td align="right">5.5</td><td align="right">19.8</td></tr><tr><td>Fumerase (Fum)</td><td align="right">7.6</td><td align="right">48.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.5</b>?</p> A. Both Fer and Fum will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Fer and Fum will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Fer will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Fum will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Fer will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Fum will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>f430</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Lactalbumin (Lac)</td><td align="right">4.3</td><td align="right">13.0</td></tr><tr><td>Streptavidin (Stp)</td><td align="right">6.0</td><td align="right">13.2</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 5.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 5.0</b>?</p> A. Both Lac and Stp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Lac and Stp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Lac will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Stp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Lac will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Stp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>351f</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Chymotrypsin (Chy)</td><td align="right">8.4</td><td align="right">25.0</td></tr><tr><td>Hexokinase (Hex)</td><td align="right">5.8</td><td align="right">120.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both Chy and Hex will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Chy and Hex will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Chy will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Hex will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Chy will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Hex will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>cf44</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Hemoglobin (Hem)</td><td align="right">7.1</td><td align="right">16.7</td></tr><tr><td>Ovalbumin (Ova)</td><td align="right">4.6</td><td align="right">45.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both Hem and Ova will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Hem and Ova will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Hem will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Ova will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Hem will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Ova will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>f4d0</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Epidermal Growth Factor (EGF)</td><td align="right">4.5</td><td align="right">40.0</td></tr><tr><td>Aldolase (Aldo)</td><td align="right">8.5</td><td align="right">47.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 9.5</b>?</p> A. Both EGF and Aldo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both EGF and Aldo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. EGF will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Aldo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. EGF will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Aldo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>8e1e</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Heat Shock Protein (HSP)</td><td align="right">5.5</td><td align="right">70.1</td></tr><tr><td>Myoglobin (Myo)</td><td align="right">7.0</td><td align="right">18.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 4.5</b>?</p> A. Both HSP and Myo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both HSP and Myo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. HSP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Myo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. HSP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Myo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>8bbd</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Insulin (Ins)</td><td align="right">5.4</td><td align="right">5.8</td></tr><tr><td>Cytochrome c (Cyt)</td><td align="right">10.2</td><td align="right">13.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both Ins and Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Ins and Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Ins will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Ins will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>099c</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Ribonuclease A (RibA)</td><td align="right">9.3</td><td align="right">13.7</td></tr><tr><td>Collagen (Col)</td><td align="right">6.6</td><td align="right">134.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both RibA and Col will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both RibA and Col will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. RibA will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Col will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. RibA will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Col will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>9ef8</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Hemoglobin (Hem)</td><td align="right">7.1</td><td align="right">16.7</td></tr><tr><td>Serum Albumin (Alb)</td><td align="right">4.9</td><td align="right">66.2</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both Hem and Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Hem and Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Hem will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Hem will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>b5ef</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Casein (Cas)</td><td align="right">4.9</td><td align="right">24.0</td></tr><tr><td>Aprotinin (Apr)</td><td align="right">9.2</td><td align="right">6.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both Cas and Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Cas and Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Cas will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Cas will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>e3d6</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Insulin (Ins)</td><td align="right">5.4</td><td align="right">5.8</td></tr><tr><td>Hemoglobin (Hem)</td><td align="right">7.1</td><td align="right">16.7</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.0</b>?</p> A. Both Ins and Hem will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Ins and Hem will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Ins will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Hem will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Ins will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Hem will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>399f</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Lipase (Lip)</td><td align="right">4.7</td><td align="right">40.0</td></tr><tr><td>Aldolase (Aldo)</td><td align="right">8.5</td><td align="right">47.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both Lip and Aldo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Lip and Aldo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Lip will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Aldo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Lip will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Aldo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>f57f</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Heat Shock Protein (HSP)</td><td align="right">5.5</td><td align="right">70.1</td></tr><tr><td>Enolase (Eno)</td><td align="right">8.4</td><td align="right">42.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 9.5</b>?</p> A. Both HSP and Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both HSP and Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. HSP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. HSP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>b53b</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Xylosidase (Xyl)</td><td align="right">5.0</td><td align="right">100.0</td></tr><tr><td>Aprotinin (Apr)</td><td align="right">9.2</td><td align="right">6.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 4.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 4.0</b>?</p> A. Both Xyl and Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Xyl and Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. Xyl will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Xyl will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>f9b7</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Aprotinin (Apr)</td><td align="right">9.2</td><td align="right">6.5</td></tr><tr><td>Histone (His)</td><td align="right">10.8</td><td align="right">15.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 10.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 10.0</b>?</p> A. Both Apr and His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Apr and His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>fffb</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>γ-Globulin (Glob)</td><td align="right">6.6</td><td align="right">57.0</td></tr><tr><td>Lactalbumin (Lac)</td><td align="right">4.3</td><td align="right">13.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both Glob and Lac will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Glob and Lac will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Glob will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Lac will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Glob will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Lac will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>8bb8</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Cytochrome c (Cyt)</td><td align="right">10.2</td><td align="right">13.0</td></tr><tr><td>Serum Albumin (Alb)</td><td align="right">4.9</td><td align="right">66.2</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 11.5</b>?</p> A. Both Cyt and Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Cyt and Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Cyt will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Alb will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Cyt will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Alb will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>b331</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Insulin (Ins)</td><td align="right">5.4</td><td align="right">5.8</td></tr><tr><td>Enolase (Eno)</td><td align="right">8.4</td><td align="right">42.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both Ins and Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Ins and Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Ins will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Ins will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>f372</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>SARS-CoV-2 Spike Protein (CoV)</td><td align="right">6.2</td><td align="right">149.9</td></tr><tr><td>Enolase (Eno)</td><td align="right">8.4</td><td align="right">42.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 5.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 5.0</b>?</p> A. Both CoV and Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both CoV and Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct C. CoV will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Eno will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. CoV will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Eno will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>af18</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Streptavidin (Stp)</td><td align="right">6.0</td><td align="right">13.2</td></tr><tr><td>Fumerase (Fum)</td><td align="right">7.6</td><td align="right">48.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both Stp and Fum will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Stp and Fum will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Stp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Fum will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Stp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Fum will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>ad49</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Histone (His)</td><td align="right">10.8</td><td align="right">15.0</td></tr><tr><td>Aprotinin (Apr)</td><td align="right">9.2</td><td align="right">6.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 10.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 10.0</b>?</p> A. Both His and Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both His and Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Apr will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Apr will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>e9d4</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Streptavidin (Stp)</td><td align="right">6.0</td><td align="right">13.2</td></tr><tr><td>Pepsin (Pep)</td><td align="right">1.0</td><td align="right">34.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 3.5</b>?</p> A. Both Stp and Pep will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Stp and Pep will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Stp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Pep will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Stp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Pep will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>5421</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>G3P Dehydrogenase (GDH)</td><td align="right">8.3</td><td align="right">36.0</td></tr><tr><td>Histone (His)</td><td align="right">10.8</td><td align="right">15.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 12.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 12.0</b>?</p> A. Both GDH and His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both GDH and His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. GDH will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. GDH will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>2c4f</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Estrogen Binding Protein (EBP)</td><td align="right">4.9</td><td align="right">115.0</td></tr><tr><td>Succinate Ligase (SL)</td><td align="right">6.6</td><td align="right">20.9</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.0</b>?</p> A. Both EBP and SL will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both EBP and SL will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. EBP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> SL will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. EBP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> SL will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>968f</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Elastase II (Ela)</td><td align="right">8.5</td><td align="right">26.5</td></tr><tr><td>Phospholypase (Pho)</td><td align="right">10.5</td><td align="right">19.5</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 11.5</b>?</p> A. Both Ela and Pho will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct B. Both Ela and Pho will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Ela will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Pho will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Ela will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Pho will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>ada8</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Phospholypase (Pho)</td><td align="right">10.5</td><td align="right">19.5</td></tr><tr><td>Ovalbumin (Ova)</td><td align="right">4.6</td><td align="right">45.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.5</b>?</p> A. Both Pho and Ova will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Pho and Ova will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Pho will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Ova will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Pho will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Ova will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>01b1</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Trypsin (Tryp)</td><td align="right">10.8</td><td align="right">23.5</td></tr><tr><td>Heat Shock Protein (HSP)</td><td align="right">5.5</td><td align="right">70.1</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.0</b>?</p> A. Both Tryp and HSP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Tryp and HSP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Tryp will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> HSP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Tryp will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> HSP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>c0ed</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Elastase II (Ela)</td><td align="right">8.5</td><td align="right">26.5</td></tr><tr><td>α-Amylase (Amy)</td><td align="right">5.9</td><td align="right">61.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 7.0</b>?</p> A. Both Ela and Amy will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Ela and Amy will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Ela will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Amy will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. Ela will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Amy will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>6ef5</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Green Fluorescent Protein (GFP)</td><td align="right">6.2</td><td align="right">28.0</td></tr><tr><td>Histone (His)</td><td align="right">10.8</td><td align="right">15.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 8.5</b>?</p> A. Both GFP and His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both GFP and His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. GFP will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> His will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. GFP will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> His will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct MC <p>1dff</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Immunoglobulin (IgG)</td><td align="right">7.3</td><td align="right">145.0</td></tr><tr><td>Insulin (Ins)</td><td align="right">5.4</td><td align="right">5.8</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.5</b>?</p> A. Both IgG and Ins will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both IgG and Ins will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. IgG will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Ins will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Correct D. IgG will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Ins will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect MC <p>5756</p> <h6>Isoelectric Point Problem</h6> <p>A mixture of two proteins are to be separated by isoelectric focusing.</p> <table cellpadding="2" cellspacing="2" style="text-align:center; border: 1px solid black;"><tr><th>Protein<br/>Name</th><th>Isoelectric<br/>Point (pI)</th><th>Molecular<br/>Weight</th></tr><tr><td>Insulin (Ins)</td><td align="right">5.4</td><td align="right">5.8</td></tr><tr><td>Myoglobin (Myo)</td><td align="right">7.0</td><td align="right">18.0</td></tr></table><p>Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.</p> <p>In which direction will each protein in the table migrate at <b>pH 6.0</b>?</p> A. Both Ins and Myo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect B. Both Ins and Myo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Incorrect C. Ins will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal<br/> Myo will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal Incorrect D. Ins will have a <span style="color:darkred">negative (–)</span> charge and travel towards the <span style="color:darkblue">positive (+)</span> terminal<br/> Myo will have a <span style="color:darkblue">positive (+)</span> charge and travel towards the <span style="color:darkred">negative (–)</span> terminal Correct