## TITLE('Biochemistry: Enzyme Inhibitor Types and Kinetic Effects')
## DESCRIPTION
## Evaluate true or false statements about competitive, non-competitive, uncompetitive, and mixed enzyme inhibitors and their effects on kinetic parameters.
## ENDDESCRIPTION
## KEYWORDS('enzyme inhibitors','competitive inhibition','non-competitive inhibition','multiple choice')
## DBsubject('Biochemistry')
## DBchapter('')
## DBsection('')
## Date('2026-07-12')
## Author('Neil R. Voss')
## Institution('Roosevelt University')
DOCUMENT();
loadMacros(
"PGstandard.pl",
"PGML.pl",
"PGchoicemacros.pl",
"parserRadioButtons.pl",
"PGcourse.pl",
);
TEXT(beginproblem());
$showPartialCorrectAnswers = 1;
#==========================================================
# AUTO-GENERATED GROUPS FROM YAML
#==========================================================
@true_groups = (
[
'Competitive inhibitors bind to the active site of an enzyme and compete with the substrate for binding.',
'Competitive inhibitors bind to the active site of an enzyme.',
'Competitive inhibitors compete with the substrate for active site binding.',
'Competitive inhibitors have a structure that is similar to the substrate.',
'Competitive inhibitors can be overcome by increasing the concentration of the substrate.',
],
[
'Noncompetitive inhibitors do NOT bind the active site, but can alter active site conformation.',
'Noncompetitive inhibitors do NOT bind the active site, but can reduce active site activity.',
'Noncompetitive inhibitors bind to a site on the enzyme that is distinct from the active site',
'Noncompetitive inhibitors do NOT affect substrate binding, but they do decrease catalysis.',
],
[
'Uncompetitive inhibitors bind to the enzyme-substrate complex and prevent product formation.',
'Uncompetitive inhibitors only bind to the enzyme-substrate complex.',
'Uncompetitive inhibitors have a higher affinity for the enzyme-substrate complex than for the free enzyme.',
],
[
'Enzyme inhibitors can be used as probes to study enzyme structure and function.',
'Enzyme inhibitors can be used as therapeutic agents to treat a variety of diseases.',
],
);
@false_groups = (
[
'Noncompetitive inhibitors bind to the active site of an enzyme and compete with the substrate for binding.',
'Noncompetitive inhibitors bind to the active site of an enzyme.',
'Noncompetitive inhibitors compete with the substrate for active site binding.',
'Noncompetitive inhibitors bind the active site to reduce active site activity.',
'Uncompetitive inhibitors bind to the active site of an enzyme and compete with the substrate for binding.',
'Uncompetitive inhibitors bind to the active site of an enzyme.',
'Uncompetitive inhibitors compete with the substrate for active site binding.',
'Uncompetitive inhibitors bind to the enzyme and prevent substrate binding.',
'Competitive inhibitors do NOT compete with the substrate for active site binding.',
'Competitive inhibitors do NOT bind to the active site of an enzyme.',
'Competitive inhibitors do NOT bind to the enzyme to prevent substrate binding.',
],
[
'Competitive inhibitors bind to a site on the enzyme that is distinct from the active site.',
'Competitive inhibitors do NOT bind the active site, but can alter active site conformation.',
'Competitive inhibitors do NOT bind the active site, but can reduce active site activity.',
'Competitive inhibitors do NOT affect substrate binding, but they do decrease catalysis.',
'Uncompetitive inhibitors bind to a site on the enzyme that is distinct from the active site.',
'Noncompetitive inhibitors affect substrate binding, but do NOT decrease catalysis.',
],
[
'Competitive inhibitors only bind to the enzyme-substrate complex.',
'Competitive inhibitors bind to the enzyme-substrate complex and prevent product formation.',
'Competitive inhibitors have a higher affinity for the enzyme-substrate complex than for the free enzyme.',
'Noncompetitive inhibitors only bind to the enzyme-substrate complex.',
'Noncompetitive inhibitors bind to the enzyme-substrate complex and prevent product formation.',
'Noncompetitive inhibitors have a higher affinity for the enzyme-substrate complex than for the free enzyme.',
'Uncompetitive inhibitors have a higher affinity for the free enzyme than for the enzyme-substrate complex.',
],
[
'Enzyme inhibitors completely block all enzyme activity regardless of their concentration.',
'Enzyme inhibitors have no impact on the structure or function of enzymes.',
],
);
#==========================================================
# GLOBAL SETTINGS
#==========================================================
$topic = 'enzyme inhibitors';
my $local_random = PGrandom->new();
$local_random->srand($problemSeed);
my @mode_choices = ("TRUE", "FALSE");
$mode = $mode_choices[$local_random->random(0, $#mode_choices, 1)];
$num_distractors = 4;
#==========================================================
# SELECT GROUP
#==========================================================
my (@selected_group, @opposite_groups);
if ($mode eq "TRUE") {
$group_index = $local_random->random(0, scalar(@true_groups)-1, 1);
@selected_group = @{ $true_groups[$group_index] };
@opposite_groups = @false_groups;
} else {
$group_index = $local_random->random(0, scalar(@false_groups)-1, 1);
@selected_group = @{ $false_groups[$group_index] };
@opposite_groups = @true_groups;
}
#==========================================================
# PICK CORRECT + DISTRACTORS
#==========================================================
my $correct_index = $local_random->random(0, $#selected_group, 1);
$correct = $selected_group[$correct_index];
my @available_group_indices = (0 .. $#opposite_groups);
my @selected_distractor_indices = ();
while (@selected_distractor_indices < $num_distractors && @available_group_indices > 0) {
my $random_index = $local_random->random(0, scalar(@available_group_indices)-1, 1);
push @selected_distractor_indices, splice(@available_group_indices, $random_index, 1);
}
@distractors = ();
foreach my $group_idx (@selected_distractor_indices) {
my @group = @{ $opposite_groups[$group_idx] };
my $distractor_index = $local_random->random(0, $#group, 1);
my $distractor = $group[$distractor_index];
push @distractors, $distractor;
}
@choices = ($correct, @distractors);
#==========================================================
# SHUFFLE CHOICES (SEED-STABLE)
#==========================================================
my @choice_indices = (0 .. $#choices);
my @choice_shuffled = ();
while (@choice_indices) {
my $pick = $local_random->random(0, $#choice_indices, 1);
push @choice_shuffled, splice(@choice_indices, $pick, 1);
}
@choices = @choices[@choice_shuffled];
#==========================================================
# RADIO BUTTONS WITH A/B/C/D/E LABELS
#==========================================================
$rb = RadioButtons(
[@choices],
$correct,
labels => ['A','B','C','D','E'],
displayLabels => 1,
randomize => 0,
separator => '',
);
#==========================================================
# PGML
#==========================================================
BEGIN_PGML
[@ $mode eq "TRUE" ? "Which one of the following statements is TRUE about $topic?" : "Which one of the following statements is FALSE about $topic?" @]*
[_]{$rb}
END_PGML
ENDDOCUMENT();