MC 4bd9_7225
Which one of the following DNA sequences below will have the LOWEST melting point (Tm).Hint: I tried to make this question pretty easy and it does not require a calculator.
| 5′– | G | A | G | , | T | G | G | , | C | C | G | , | G | C | C | –3′ |
| 3′– | C | T | C | , | A | C | C | , | G | G | C | , | C | G | G | –5′ |
Incorrect | 5′– | C | T | C | , | G | C | C | , | T | C | T | , | C | G | C | –3′ |
| 3′– | G | A | G | , | C | G | G | , | A | G | A | , | G | C | G | –5′ |
Incorrect | 5′– | T | T | A | , | A | T | G | , | C | T | A | , | T | A | T | –3′ |
| 3′– | A | A | T | , | T | A | C | , | G | A | T | , | A | T | A | –5′ |
Correct | 5′– | C | T | G | , | C | A | C | , | G | G | A | , | G | T | C | –3′ |
| 3′– | G | A | C | , | G | T | G | , | C | C | T | , | C | A | G | –5′ |
Incorrect
MC f4a9_7510
Which one of the following DNA sequences below will have the HIGHEST melting point (Tm).Hint: I tried to make this question pretty easy and it does not require a calculator.
| 5′– | A | T | T | , | A | T | A | , | T | A | T | , | G | T | C | –3′ |
| 3′– | T | A | A | , | T | A | T | , | A | T | A | , | C | A | G | –5′ |
Incorrect | 5′– | G | T | G | , | T | A | T | , | A | G | A | , | A | T | T | –3′ |
| 3′– | C | A | C | , | A | T | A | , | T | C | T | , | T | A | A | –5′ |
Incorrect | 5′– | A | C | C | , | T | A | C | , | C | T | G | , | C | G | G | –3′ |
| 3′– | T | G | G | , | A | T | G | , | G | A | C | , | G | C | C | –5′ |
Correct | 5′– | A | A | C | , | T | G | T | , | T | A | G | , | T | T | G | –3′ |
| 3′– | T | T | G | , | A | C | A | , | A | T | C | , | A | A | C | –5′ |
Incorrect