NUM

44ad_3dda

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.2	22.3	19.8	21.9
Well 2	19.1	23.6	18.6	22.6
Well 3	18.5	23.5	18.9	21.7
mean Ct	18.9	23.1	19.1	22.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.30000000 0.05000000 NUM

1369_8bfb

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.8	22.3	19.7	19.4
Well 2	19.3	22.0	19.2	19.2
Well 3	18.5	22.0	19.6	19.4
mean Ct	18.9	22.1	19.5	19.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

10.56000000 0.05000000 NUM

33d6_3dda

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	22.0	29.1	21.1	28.0
Well 2	21.4	29.8	21.1	27.1
Well 3	21.6	29.4	21.4	28.0
mean Ct	21.7	29.4	21.2	27.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.30000000 0.05000000 NUM

83ee_6427

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.5	23.5	18.5	20.3
Well 2	18.6	24.2	18.5	19.7
Well 3	18.8	23.6	19.0	19.3
mean Ct	18.3	23.8	18.7	19.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

21.11000000 0.05000000 NUM

8f65_2c55

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	22.2	26.5	22.3	25.4
Well 2	22.0	26.4	21.7	25.8
Well 3	21.2	25.9	21.8	26.2
mean Ct	21.8	26.3	21.9	25.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.52000000 0.05000000 NUM

5c07_6aeb

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.8	21.9	19.9	22.9
Well 2	19.3	21.6	19.2	24.2
Well 3	18.2	21.9	19.3	22.9
mean Ct	18.8	21.8	19.5	23.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.74000000 0.05000000 NUM

4639_5927

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	16.5	25.2	16.8	20.6
Well 2	17.1	25.0	17.3	20.8
Well 3	16.5	25.3	18.0	22.0
mean Ct	16.7	25.2	17.4	21.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

27.86000000 0.05000000 NUM

2349_19f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.8	26.6	20.7	29.7
Well 2	19.9	26.6	20.4	29.1
Well 3	20.2	26.5	20.6	28.4
mean Ct	20.3	26.6	20.6	29.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.59000000 0.05000000 NUM

45c1_5a7c

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.5	24.6	20.7	20.1
Well 2	19.6	25.1	20.7	20.5
Well 3	20.4	24.1	20.3	21.7
mean Ct	20.2	24.6	20.6	20.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

18.38000000 0.05000000 NUM

db0d_a839

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.3	26.5	19.8	30.2
Well 2	18.9	27.2	18.7	30.6
Well 3	18.7	27.7	19.7	29.8
mean Ct	19.0	27.1	19.4	30.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.50000000 0.05000000 NUM

ef1d_9610

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.5	21.9	17.2	18.7
Well 2	17.4	21.1	18.3	19.6
Well 3	17.6	21.7	17.6	18.5
mean Ct	17.8	21.6	17.7	18.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.06000000 0.05000000 NUM

a605_b89a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.8	26.6	20.9	26.0
Well 2	20.6	25.2	20.3	26.2
Well 3	21.8	26.3	20.7	26.9
mean Ct	21.1	26.0	20.6	26.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.87000000 0.05000000 NUM

2bbc_a3c3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.4	26.2	19.7	30.1
Well 2	19.6	25.5	20.1	29.5
Well 3	19.3	25.5	20.9	30.4
mean Ct	19.4	25.7	20.2	30.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

11.31000000 0.05000000 NUM

4d06_0ae3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.1	25.6	20.2	22.5
Well 2	20.7	24.5	20.1	22.2
Well 3	20.2	25.3	20.9	22.4
mean Ct	20.7	25.1	20.4	22.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.28000000 0.05000000 NUM

755f_cf70

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.0	26.1	21.1	26.3
Well 2	20.4	26.0	20.8	26.3
Well 3	20.8	25.0	20.7	27.1
mean Ct	20.7	25.7	20.9	26.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.62000000 0.05000000 NUM

4999_0f0a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.5	22.8	18.2	22.0
Well 2	18.3	23.0	18.3	22.5
Well 3	18.0	22.4	17.7	22.6
mean Ct	17.9	22.7	18.1	22.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.41000000 0.05000000 NUM

f136_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.3	27.9	21.4	31.5
Well 2	19.7	27.1	20.7	30.4
Well 3	20.4	28.0	21.7	30.8
mean Ct	20.1	27.7	21.3	30.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

137a_a3c3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.2	25.1	21.0	28.9
Well 2	21.6	26.4	22.5	29.5
Well 3	22.2	25.3	21.9	29.2
mean Ct	21.7	25.6	21.8	29.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

11.31000000 0.05000000 NUM

dc8c_a3c3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.1	23.0	17.0	24.8
Well 2	17.8	22.4	17.7	25.5
Well 3	17.8	22.0	17.1	25.8
mean Ct	17.9	22.5	17.3	25.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

11.31000000 0.05000000 NUM

8e90_1d40

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.1	26.4	19.7	24.1
Well 2	19.5	26.9	19.4	23.8
Well 3	19.4	26.8	20.5	25.2
mean Ct	19.3	26.7	19.9	24.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

7.46000000 0.05000000 NUM

d0f4_6aeb

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.2	27.7	22.5	26.1
Well 2	21.8	27.1	22.4	27.0
Well 3	22.0	26.6	21.4	27.1
mean Ct	21.7	27.1	22.1	26.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.74000000 0.05000000 NUM

5c28_deff

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.9	24.6	20.8	24.9
Well 2	21.7	24.6	20.7	24.8
Well 3	20.6	23.7	21.0	25.3
mean Ct	21.1	24.3	20.8	25.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.00000000 0.05000000 NUM

8f4b_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.2	26.3	20.7	28.3
Well 2	20.1	26.2	20.0	27.4
Well 3	21.4	25.3	19.9	27.3
mean Ct	20.9	25.9	20.2	27.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

4010_b624

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.2	24.9	17.0	20.3
Well 2	16.8	24.9	16.7	21.4
Well 3	17.5	25.3	16.8	21.2
mean Ct	17.2	25.0	16.8	21.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

12.13000000 0.05000000 NUM

2f47_19f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.5	22.5	19.2	22.0
Well 2	17.8	22.5	19.4	21.9
Well 3	18.5	23.4	19.4	22.2
mean Ct	17.9	22.8	19.3	22.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.59000000 0.05000000 NUM

1439_a3c3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.8	23.0	19.5	19.6
Well 2	19.8	22.6	20.3	20.3
Well 3	18.4	22.2	18.9	19.3
mean Ct	19.0	22.6	19.6	19.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

11.31000000 0.05000000 NUM

45f9_56f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.2	24.8	17.1	28.4
Well 2	18.1	25.1	17.1	28.9
Well 3	17.8	24.7	17.9	28.5
mean Ct	17.7	24.9	17.4	28.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

16.00000000 0.05000000 NUM

f206_1fa9

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.2	23.4	19.7	19.8
Well 2	19.8	22.8	18.8	19.7
Well 3	19.3	22.7	18.9	20.1
mean Ct	19.4	23.0	19.1	19.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.96000000 0.05000000 NUM

45af_f3b3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.9	27.9	21.4	32.0
Well 2	22.0	29.0	21.9	32.3
Well 3	20.9	28.5	20.9	31.9
mean Ct	21.6	28.5	21.4	32.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

13.93000000 0.05000000 NUM

65cf_a839

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.4	24.0	20.4	26.5
Well 2	20.5	24.5	20.4	27.3
Well 3	20.6	24.6	21.9	27.9
mean Ct	20.8	24.4	20.9	27.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.50000000 0.05000000 NUM

112d_3d63

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.5	27.3	21.0	31.5
Well 2	20.9	27.8	21.2	30.8
Well 3	20.6	28.2	22.0	31.6
mean Ct	21.0	27.8	21.4	31.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

8.57000000 0.05000000 NUM

08f2_3b29

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.5	23.5	19.4	25.5
Well 2	19.4	22.7	18.5	25.1
Well 3	18.9	23.7	18.8	25.1
mean Ct	18.9	23.3	18.9	25.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.73000000 0.05000000 NUM

1f9e_563f

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.5	25.8	20.2	27.7
Well 2	21.1	25.6	19.7	28.8
Well 3	20.8	25.5	20.6	28.0
mean Ct	20.8	25.6	20.2	28.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

9.19000000 0.05000000 NUM

c065_63b9

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.9	23.1	19.0	26.1
Well 2	19.2	22.6	19.9	26.2
Well 3	20.1	23.2	19.7	27.2
mean Ct	19.7	23.0	19.5	26.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

13.00000000 0.05000000 NUM

6ed3_b624

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	22.0	25.8	22.4	30.1
Well 2	21.8	25.7	22.8	29.1
Well 3	22.5	25.5	21.6	29.4
mean Ct	22.1	25.7	22.3	29.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

12.13000000 0.05000000 NUM

528c_0500

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.6	24.9	18.1	23.5
Well 2	18.1	25.6	19.6	24.4
Well 3	18.7	25.1	18.8	23.4
mean Ct	18.5	25.2	18.8	23.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.25000000 0.05000000 NUM

ea49_c948

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.9	26.5	20.1	29.6
Well 2	21.8	26.5	21.1	29.3
Well 3	20.6	26.6	21.5	29.8
mean Ct	21.1	26.5	20.9	29.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

9.85000000 0.05000000 NUM

13db_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.0	24.2	19.9	27.2
Well 2	19.0	23.8	19.5	26.5
Well 3	19.0	23.9	19.3	26.7
mean Ct	19.3	24.0	19.6	26.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

bb0d_b624

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.1	26.5	21.2	23.0
Well 2	21.1	26.7	21.1	23.7
Well 3	20.5	26.6	21.3	23.2
mean Ct	20.9	26.6	21.2	23.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

12.13000000 0.05000000 NUM

67b4_563f

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.3	25.9	20.3	30.0
Well 2	19.3	25.7	19.6	30.0
Well 3	19.5	27.0	19.6	29.3
mean Ct	19.4	26.2	19.8	29.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

9.19000000 0.05000000 NUM

6eee_8da6

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.1	25.5	17.4	23.7
Well 2	18.6	24.6	18.9	23.0
Well 3	19.1	25.6	18.6	23.9
mean Ct	18.9	25.2	18.3	23.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.14000000 0.05000000 NUM

56ac_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.8	23.9	20.1	26.8
Well 2	20.5	24.7	20.8	26.0
Well 3	20.2	24.1	20.2	25.4
mean Ct	20.5	24.2	20.4	26.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

04fb_836a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.0	23.7	20.0	19.4
Well 2	20.0	23.3	18.9	19.2
Well 3	18.7	22.8	19.5	19.9
mean Ct	19.2	23.3	19.5	19.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

17.15000000 0.05000000 NUM

7822_b89a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.4	23.7	21.2	24.1
Well 2	21.0	24.8	21.1	23.3
Well 3	20.3	24.0	20.4	23.5
mean Ct	20.6	24.2	20.9	23.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.87000000 0.05000000 NUM

226e_deff

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.7	24.3	20.3	26.2
Well 2	19.5	25.6	20.1	25.6
Well 3	20.2	24.8	19.3	26.1
mean Ct	19.8	24.9	19.9	26.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.00000000 0.05000000 NUM

b462_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.4	25.6	18.0	27.8
Well 2	18.5	26.5	18.8	28.8
Well 3	18.4	25.6	18.2	28.4
mean Ct	18.4	25.9	18.3	28.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

1ea8_f3b3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.0	25.7	19.7	21.7
Well 2	19.2	26.0	20.3	22.7
Well 3	19.7	25.7	19.0	21.9
mean Ct	19.6	25.8	19.7	22.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

13.93000000 0.05000000 NUM

a13e_0500

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.3	25.4	19.7	26.6
Well 2	20.4	25.1	20.2	26.6
Well 3	19.7	24.9	19.7	26.7
mean Ct	20.1	25.1	19.9	26.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.25000000 0.05000000 NUM

c172_8da6

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.9	25.4	20.2	26.3
Well 2	21.3	25.3	21.0	26.3
Well 3	20.8	24.9	20.9	25.4
mean Ct	21.0	25.2	20.7	26.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.14000000 0.05000000 NUM

fcd1_c948

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.3	22.4	18.4	18.3
Well 2	18.5	22.1	19.4	18.5
Well 3	18.7	22.1	18.3	19.6
mean Ct	18.8	22.2	18.7	18.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

9.85000000 0.05000000 NUM

0ec0_6aeb

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.5	25.5	20.8	24.9
Well 2	20.2	26.1	20.6	25.2
Well 3	20.0	25.0	19.9	24.7
mean Ct	20.2	25.5	20.4	24.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.74000000 0.05000000 NUM

e42d_7419

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.3	28.7	21.1	31.0
Well 2	21.3	28.1	21.9	30.3
Well 3	20.7	29.0	22.3	31.0
mean Ct	21.1	28.6	21.8	30.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

2.83000000 0.05000000 NUM

9d8c_1d40

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.4	28.7	21.5	32.5
Well 2	20.5	28.7	21.8	32.8
Well 3	20.7	28.4	22.4	32.3
mean Ct	20.9	28.6	21.9	32.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

7.46000000 0.05000000 NUM

88f1_a809

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	16.7	22.4	18.1	18.7
Well 2	16.9	21.6	17.6	17.7
Well 3	17.4	22.7	17.6	17.9
mean Ct	17.0	22.2	17.8	18.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

29.86000000 0.05000000 NUM

879f_3b29

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.3	22.5	17.7	21.2
Well 2	17.6	22.6	17.8	21.0
Well 3	18.2	23.7	18.1	20.6
mean Ct	18.0	22.9	17.9	20.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.73000000 0.05000000 NUM

208b_a3c3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.3	29.4	21.1	25.4
Well 2	20.9	28.9	20.9	26.4
Well 3	20.9	29.6	20.7	25.3
mean Ct	21.0	29.3	20.9	25.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

11.31000000 0.05000000 NUM

2138_3dda

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.7	26.5	19.5	24.2
Well 2	20.8	27.1	20.4	24.7
Well 3	21.0	26.8	19.7	25.3
mean Ct	20.8	26.8	19.9	24.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.30000000 0.05000000 NUM

b004_836a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.1	22.9	21.1	26.8
Well 2	20.4	23.8	20.1	27.3
Well 3	21.0	24.0	19.9	27.7
mean Ct	20.8	23.6	20.4	27.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

17.15000000 0.05000000 NUM

ca16_0ae3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.8	25.0	18.0	26.7
Well 2	18.2	25.0	18.3	27.3
Well 3	19.0	25.5	18.6	27.5
mean Ct	18.7	25.2	18.3	27.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.28000000 0.05000000 NUM

b0cb_56f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.8	21.9	16.0	25.1
Well 2	17.2	21.4	16.1	24.9
Well 3	16.8	21.1	17.1	23.9
mean Ct	17.3	21.5	16.4	24.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

16.00000000 0.05000000 NUM

049b_bec4

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.5	21.5	17.8	22.7
Well 2	18.4	20.4	18.1	22.9
Well 3	17.5	20.8	17.7	22.8
mean Ct	17.8	20.9	17.9	22.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.48000000 0.05000000 NUM

c7a2_1fa9

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.8	27.5	19.5	30.0
Well 2	19.4	27.7	18.7	29.9
Well 3	20.3	27.7	19.4	30.5
mean Ct	19.5	27.6	19.2	30.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.96000000 0.05000000 NUM

6937_9610

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.7	29.3	21.3	26.8
Well 2	21.0	29.1	21.7	26.7
Well 3	22.1	29.4	21.9	26.5
mean Ct	21.6	29.3	21.6	26.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.06000000 0.05000000 NUM

f44c_8da6

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.2	25.7	18.9	25.5
Well 2	20.3	26.9	19.2	27.0
Well 3	18.9	25.9	18.8	27.0
mean Ct	19.8	26.2	19.0	26.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.14000000 0.05000000 NUM

3dfb_8da6

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.0	25.0	18.3	26.3
Well 2	19.4	25.8	19.5	26.3
Well 3	18.9	25.3	19.2	26.7
mean Ct	19.1	25.4	19.0	26.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.14000000 0.05000000 NUM

6826_4e2a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.5	25.7	20.8	26.9
Well 2	21.7	26.2	20.8	26.9
Well 3	21.0	25.5	22.0	26.8
mean Ct	21.4	25.8	21.2	26.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.46000000 0.05000000 NUM

b930_3b29

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.0	21.7	16.8	19.1
Well 2	17.9	20.8	17.4	18.8
Well 3	16.7	20.9	17.1	18.6
mean Ct	17.5	21.1	17.1	18.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.73000000 0.05000000 NUM

5a5d_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.9	25.0	18.5	21.9
Well 2	19.6	24.1	18.9	23.1
Well 3	18.8	24.1	19.5	22.0
mean Ct	19.1	24.4	19.0	22.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

526a_6aeb

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.1	27.4	19.9	28.0
Well 2	19.8	26.7	19.3	28.3
Well 3	19.6	27.6	19.4	27.8
mean Ct	19.5	27.2	19.5	28.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.74000000 0.05000000 NUM

f14f_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.1	25.3	19.4	26.1
Well 2	19.6	24.4	18.1	27.2
Well 3	18.7	24.3	18.5	27.1
mean Ct	19.1	24.7	18.7	26.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

83f3_63b9

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.0	22.7	19.0	26.4
Well 2	18.8	22.1	18.8	26.1
Well 3	18.7	23.3	18.6	26.6
mean Ct	18.8	22.7	18.8	26.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

13.00000000 0.05000000 NUM

59c2_bec4

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.2	24.1	20.8	23.3
Well 2	20.9	24.1	21.5	22.9
Well 3	20.9	25.0	21.6	22.6
mean Ct	21.0	24.4	21.3	22.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.48000000 0.05000000 NUM

5384_0f0a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.3	23.1	17.9	23.1
Well 2	16.9	24.1	16.8	23.0
Well 3	17.3	23.4	18.1	23.1
mean Ct	17.5	23.5	17.6	23.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.41000000 0.05000000 NUM

df44_782b

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.9	21.5	18.5	25.6
Well 2	17.3	21.0	18.7	25.9
Well 3	17.6	21.9	18.5	24.9
mean Ct	17.6	21.5	18.6	25.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

8.00000000 0.05000000 NUM

018e_56f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.6	27.5	20.3	23.0
Well 2	21.0	27.0	21.2	23.0
Well 3	21.4	27.0	21.1	23.3
mean Ct	21.0	27.2	20.9	23.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

16.00000000 0.05000000 NUM

5f8e_4e2a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	22.1	26.4	21.5	27.0
Well 2	21.4	26.6	21.2	27.0
Well 3	22.3	25.4	22.4	27.5
mean Ct	21.9	26.1	21.7	27.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.46000000 0.05000000 NUM

0633_43be

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	22.0	26.4	22.2	29.0
Well 2	21.8	25.7	22.4	28.0
Well 3	21.0	26.1	22.5	28.6
mean Ct	21.6	26.1	22.4	28.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.03000000 0.05000000 NUM

f62a_8bfb

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.2	28.9	20.7	25.6
Well 2	21.3	28.9	21.3	25.8
Well 3	21.6	28.5	21.9	25.8
mean Ct	21.0	28.8	21.3	25.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

10.56000000 0.05000000 NUM

d47f_56f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.2	21.8	18.7	17.7
Well 2	17.1	20.5	18.3	18.5
Well 3	17.7	21.8	17.7	18.7
mean Ct	17.3	21.4	18.2	18.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

16.00000000 0.05000000 NUM

e468_a839

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.0	20.9	18.2	18.2
Well 2	17.9	20.8	17.9	18.4
Well 3	18.1	21.0	17.4	17.5
mean Ct	18.0	20.9	17.8	18.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.50000000 0.05000000 NUM

649b_b89a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.3	23.3	18.4	21.8
Well 2	18.2	22.5	18.3	21.6
Well 3	18.1	22.0	18.5	21.5
mean Ct	18.5	22.6	18.4	21.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.87000000 0.05000000 NUM

9fdb_a3c3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.9	26.4	19.8	23.9
Well 2	20.1	27.0	20.7	23.1
Well 3	19.8	25.8	20.7	23.1
mean Ct	19.9	26.4	20.4	23.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

11.31000000 0.05000000 NUM

58bd_9610

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.0	26.2	18.8	23.9
Well 2	18.9	26.1	18.2	23.5
Well 3	18.5	26.0	18.6	23.1
mean Ct	18.5	26.1	18.5	23.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.06000000 0.05000000 NUM

300f_3b29

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.1	28.2	21.4	26.2
Well 2	21.2	29.1	21.7	26.5
Well 3	22.1	29.4	20.3	27.2
mean Ct	21.5	28.9	21.1	26.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.73000000 0.05000000 NUM

30b7_0500

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.2	23.3	18.4	21.9
Well 2	17.3	23.4	17.6	23.0
Well 3	17.4	23.1	18.3	22.4
mean Ct	17.3	23.3	18.1	22.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.25000000 0.05000000 NUM

6357_b89a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.6	26.2	18.5	24.7
Well 2	18.5	25.1	18.8	25.0
Well 3	17.7	26.0	18.6	25.9
mean Ct	18.3	25.8	18.6	25.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.87000000 0.05000000 NUM

dee0_3b29

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.4	22.9	19.5	24.1
Well 2	19.2	22.1	19.4	24.5
Well 3	19.2	21.7	19.7	24.4
mean Ct	19.3	22.2	19.5	24.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.73000000 0.05000000 NUM

b2f2_3b29

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.5	22.5	17.3	23.0
Well 2	17.0	21.3	18.1	24.1
Well 3	17.8	22.2	17.0	23.6
mean Ct	17.8	22.0	17.5	23.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.73000000 0.05000000 NUM

2bfe_f531

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	22.7	29.1	21.9	29.9
Well 2	21.9	28.9	21.5	29.2
Well 3	22.5	28.1	21.6	29.1
mean Ct	22.4	28.7	21.7	29.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

2.64000000 0.05000000 NUM

c93c_cf70

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.1	25.9	20.7	23.7
Well 2	21.3	24.8	19.9	23.9
Well 3	20.2	25.6	20.3	24.7
mean Ct	20.9	25.4	20.3	24.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.62000000 0.05000000 NUM

a814_6aeb

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.8	23.1	18.1	23.4
Well 2	17.9	22.9	17.9	23.8
Well 3	18.8	22.3	18.3	23.3
mean Ct	18.2	22.8	18.1	23.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.74000000 0.05000000 NUM

b2c3_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.0	26.2	16.9	22.7
Well 2	18.1	25.0	17.7	22.8
Well 3	18.0	25.1	18.1	21.9
mean Ct	18.0	25.4	17.6	22.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

3841_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.4	23.4	19.6	26.3
Well 2	18.9	23.9	20.2	26.2
Well 3	20.0	22.8	19.2	26.0
mean Ct	19.4	23.4	19.7	26.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

f6f0_bec4

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.4	26.5	20.6	29.0
Well 2	19.1	26.9	20.1	28.5
Well 3	20.1	26.9	20.1	29.5
mean Ct	19.9	26.8	20.3	29.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.48000000 0.05000000 NUM

8d1c_f531

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	16.8	23.2	17.7	24.0
Well 2	17.9	23.3	16.6	24.0
Well 3	17.9	22.6	16.7	23.6
mean Ct	17.5	23.0	17.0	23.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

2.64000000 0.05000000 NUM

aa9f_782b

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.5	25.9	16.9	22.3
Well 2	16.9	25.0	17.2	22.2
Well 3	18.3	25.1	17.5	21.1
mean Ct	17.6	25.3	17.2	21.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

8.00000000 0.05000000 NUM

07db_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.8	25.5	22.1	22.7
Well 2	21.6	24.6	21.9	23.1
Well 3	21.8	25.4	21.4	22.7
mean Ct	21.7	25.2	21.8	22.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

6a27_63b9

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.4	25.4	19.0	30.3
Well 2	19.2	26.5	19.0	29.9
Well 3	19.8	26.5	19.3	29.2
mean Ct	19.1	26.1	19.1	29.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

13.00000000 0.05000000 NUM

ece5_c3c8

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	22.2	25.7	21.5	27.9
Well 2	21.8	25.6	21.4	28.1
Well 3	21.5	25.5	22.1	27.5
mean Ct	21.8	25.6	21.7	27.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.92000000 0.05000000 NUM

6016_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.4	25.5	22.4	28.5
Well 2	22.3	25.9	22.8	28.3
Well 3	22.3	26.2	22.0	28.1
mean Ct	22.0	25.9	22.4	28.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

e52d_8da6

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.8	25.2	20.2	26.7
Well 2	20.8	26.3	19.7	26.2
Well 3	20.6	25.4	20.7	25.6
mean Ct	20.7	25.6	20.2	26.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.14000000 0.05000000 NUM

5bae_6aeb

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.2	25.7	19.1	26.1
Well 2	18.6	25.2	18.8	26.8
Well 3	18.4	25.0	19.4	26.5
mean Ct	18.7	25.3	19.1	26.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.74000000 0.05000000 NUM

27d1_bec4

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.9	25.5	21.5	23.9
Well 2	21.9	24.9	22.5	24.2
Well 3	21.8	24.6	22.4	23.3
mean Ct	21.5	25.0	22.1	23.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.48000000 0.05000000 NUM

9963_9610

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.5	22.3	17.7	25.0
Well 2	18.9	21.6	18.3	23.6
Well 3	17.6	22.2	18.0	24.4
mean Ct	18.3	22.0	18.0	24.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.06000000 0.05000000 NUM

1c9c_7419

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	16.6	22.8	16.5	21.4
Well 2	17.7	22.7	17.6	21.1
Well 3	17.4	23.8	16.7	21.5
mean Ct	17.2	23.1	16.9	21.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

2.83000000 0.05000000 NUM

01d6_43be

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	22.0	29.0	22.1	28.1
Well 2	22.5	29.8	22.9	27.7
Well 3	22.0	28.9	22.0	27.2
mean Ct	22.2	29.2	22.3	27.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.03000000 0.05000000 NUM

7dc0_c948

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.6	26.8	18.5	30.6
Well 2	19.2	26.2	19.5	30.2
Well 3	18.8	26.6	19.8	29.8
mean Ct	18.9	26.5	19.3	30.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

9.85000000 0.05000000 NUM

c863_19f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.4	26.8	21.6	29.2
Well 2	21.0	25.5	21.5	30.0
Well 3	19.8	26.9	20.6	28.9
mean Ct	20.4	26.4	21.2	29.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.59000000 0.05000000 NUM

3a67_2c55

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.7	24.7	17.5	24.1
Well 2	18.3	24.7	17.3	24.1
Well 3	17.8	25.5	18.0	24.2
mean Ct	17.9	25.0	17.6	24.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.52000000 0.05000000 NUM

42ab_1d40

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.5	20.1	17.7	22.9
Well 2	17.3	20.5	17.4	23.0
Well 3	17.1	20.7	16.8	24.0
mean Ct	17.3	20.4	17.3	23.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

7.46000000 0.05000000 NUM

5dcc_56f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.3	28.9	20.3	32.0
Well 2	21.4	28.5	20.7	32.6
Well 3	21.4	28.6	21.3	31.6
mean Ct	21.4	28.7	20.8	32.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

16.00000000 0.05000000 NUM

f81f_563f

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.1	27.5	19.3	23.6
Well 2	18.6	26.5	18.7	23.6
Well 3	19.8	27.7	19.3	23.5
mean Ct	19.5	27.2	19.1	23.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

9.19000000 0.05000000 NUM

5f18_1fa9

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.2	24.0	20.5	27.5
Well 2	20.1	24.6	21.7	28.0
Well 3	20.8	25.4	20.2	27.2
mean Ct	20.7	24.7	20.8	27.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.96000000 0.05000000 NUM

1563_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.4	23.4	18.3	25.3
Well 2	18.2	24.2	18.0	25.1
Well 3	18.6	23.3	17.8	24.4
mean Ct	18.7	23.6	18.0	24.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

cf47_f3b3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.8	29.4	20.4	25.8
Well 2	20.5	29.8	20.4	25.6
Well 3	21.0	29.7	21.6	26.1
mean Ct	20.8	29.6	20.8	25.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

13.93000000 0.05000000 NUM

e632_cf70

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.1	29.1	21.6	31.3
Well 2	21.0	28.6	22.0	30.5
Well 3	20.1	29.5	21.0	30.8
mean Ct	20.4	29.1	21.5	30.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.62000000 0.05000000 NUM

abcb_b624

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.1	23.3	18.8	26.8
Well 2	18.4	22.5	18.4	26.2
Well 3	18.4	22.8	18.2	27.1
mean Ct	18.3	22.9	18.5	26.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

12.13000000 0.05000000 NUM

b493_f531

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.1	24.0	18.8	24.2
Well 2	18.6	24.7	19.2	23.2
Well 3	18.8	24.1	20.2	23.2
mean Ct	18.8	24.3	19.4	23.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

2.64000000 0.05000000 NUM

21b8_0500

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.7	22.1	17.2	19.7
Well 2	18.3	22.1	17.2	20.5
Well 3	17.2	21.9	17.6	19.4
mean Ct	17.7	22.0	17.3	19.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.25000000 0.05000000 NUM

3c2a_3dda

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.2	21.9	17.7	22.6
Well 2	18.0	22.2	17.3	23.3
Well 3	18.4	22.3	17.6	22.9
mean Ct	17.9	22.1	17.5	22.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.30000000 0.05000000 NUM

4fc8_3dda

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.0	21.7	18.3	20.8
Well 2	18.6	21.5	18.3	20.4
Well 3	19.2	22.3	18.3	19.7
mean Ct	18.6	21.8	18.3	20.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.30000000 0.05000000 NUM

0f21_6427

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.7	25.3	21.5	20.5
Well 2	20.9	25.4	20.2	22.0
Well 3	20.1	25.7	20.8	21.5
mean Ct	20.6	25.5	20.8	21.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

21.11000000 0.05000000 NUM

9925_7419

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.7	25.1	20.5	22.4
Well 2	19.8	24.0	19.9	23.1
Well 3	20.5	23.9	20.7	23.1
mean Ct	20.3	24.3	20.4	22.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

2.83000000 0.05000000 NUM

ac0b_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.9	22.7	19.3	26.1
Well 2	19.3	22.8	20.5	25.2
Well 3	19.6	23.6	20.5	25.2
mean Ct	19.6	23.0	20.1	25.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

58a1_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.9	20.8	17.2	22.4
Well 2	17.3	21.0	18.3	23.1
Well 3	18.0	20.6	18.4	23.7
mean Ct	17.7	20.8	18.0	23.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

e247_3b29

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.5	26.7	21.4	27.9
Well 2	22.1	26.6	21.2	28.8
Well 3	22.0	26.6	22.3	27.8
mean Ct	21.9	26.6	21.6	28.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.73000000 0.05000000 NUM

cfcd_deff

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.5	28.3	21.1	29.7
Well 2	21.1	27.8	21.5	30.0
Well 3	20.8	28.6	21.1	29.1
mean Ct	20.8	28.2	21.2	29.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.00000000 0.05000000 NUM

c994_4e2a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.6	29.2	22.3	30.3
Well 2	20.4	28.6	20.9	30.5
Well 3	20.7	28.4	21.1	30.6
mean Ct	20.9	28.7	21.4	30.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.46000000 0.05000000 NUM

8feb_c948

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.4	26.2	21.0	28.0
Well 2	21.5	25.7	21.0	29.2
Well 3	21.9	24.8	22.1	28.9
mean Ct	21.6	25.6	21.4	28.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

9.85000000 0.05000000 NUM

cbbd_563f

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.3	26.9	19.1	30.6
Well 2	19.2	26.8	19.7	29.8
Well 3	18.9	26.2	19.1	29.7
mean Ct	19.1	26.6	19.3	30.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

9.19000000 0.05000000 NUM

fb2a_b624

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.3	22.9	19.8	27.0
Well 2	20.6	23.7	20.8	26.7
Well 3	20.5	23.7	20.3	27.8
mean Ct	20.1	23.4	20.3	27.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

12.13000000 0.05000000 NUM

f887_56f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.1	27.2	22.1	23.6
Well 2	21.3	27.2	21.9	23.7
Well 3	21.3	26.4	21.2	22.8
mean Ct	21.2	26.9	21.7	23.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

16.00000000 0.05000000 NUM

eb08_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.0	22.7	20.1	20.7
Well 2	20.2	23.2	19.3	20.4
Well 3	20.1	22.7	19.2	19.7
mean Ct	20.1	22.9	19.5	20.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

5fcd_782b

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.1	25.8	19.4	22.9
Well 2	19.5	26.1	19.3	22.4
Well 3	19.5	25.9	19.1	23.1
mean Ct	19.4	25.9	19.3	22.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

8.00000000 0.05000000 NUM

bfc1_b624

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.3	27.4	21.1	24.5
Well 2	21.3	28.2	20.3	24.1
Well 3	20.0	28.1	21.7	24.7
mean Ct	20.9	27.9	21.0	24.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

12.13000000 0.05000000 NUM

2757_8bfb

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.8	24.0	20.5	28.0
Well 2	21.2	25.0	19.6	26.6
Well 3	20.5	24.9	19.3	27.3
mean Ct	20.5	24.6	19.8	27.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

10.56000000 0.05000000 NUM

33d3_43be

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.6	26.2	21.4	25.3
Well 2	20.1	26.4	21.2	25.5
Well 3	20.1	26.0	20.9	25.7
mean Ct	20.3	26.2	21.2	25.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.03000000 0.05000000 NUM

ba6c_a3c3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.2	25.4	20.1	28.6
Well 2	20.3	25.3	20.5	29.2
Well 3	19.9	24.6	21.0	29.2
mean Ct	20.1	25.1	20.5	29.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

11.31000000 0.05000000 NUM

bb67_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.7	26.4	21.1	23.8
Well 2	20.8	26.0	20.0	24.0
Well 3	20.7	26.6	21.4	24.3
mean Ct	21.1	26.3	20.8	24.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

5783_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.1	21.8	17.4	19.7
Well 2	17.8	22.9	18.6	20.7
Well 3	17.8	22.5	17.4	20.4
mean Ct	17.9	22.4	17.8	20.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

64cd_c3c8

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.8	29.6	20.8	31.1
Well 2	21.0	28.6	21.1	31.5
Well 3	21.1	29.0	21.5	30.9
mean Ct	21.3	29.1	21.1	31.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.92000000 0.05000000 NUM

77e5_8da6

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.3	24.8	20.9	27.8
Well 2	20.2	26.3	21.4	27.3
Well 3	20.4	25.1	20.8	26.4
mean Ct	20.3	25.4	21.0	27.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.14000000 0.05000000 NUM

4bd5_6427

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.9	24.6	19.4	28.8
Well 2	19.1	24.8	20.1	29.0
Well 3	19.2	24.4	18.6	29.2
mean Ct	19.4	24.6	19.4	29.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

21.11000000 0.05000000 NUM

83e3_19f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.4	27.6	19.7	25.5
Well 2	18.8	28.0	19.3	25.9
Well 3	19.5	27.3	19.8	25.9
mean Ct	19.2	27.6	19.6	25.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.59000000 0.05000000 NUM

f2ca_b89a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.7	29.3	22.4	29.5
Well 2	21.2	28.6	21.1	30.7
Well 3	21.2	29.2	21.6	30.3
mean Ct	21.4	29.0	21.7	30.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.87000000 0.05000000 NUM

4811_b624

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.3	24.9	18.1	21.3
Well 2	19.4	25.9	18.8	22.4
Well 3	18.4	26.3	19.1	21.8
mean Ct	19.0	25.7	18.7	21.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

12.13000000 0.05000000 NUM

e55a_19f7

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.8	24.9	20.2	28.0
Well 2	20.1	25.4	19.5	27.2
Well 3	18.8	24.1	19.5	27.2
mean Ct	19.2	24.8	19.7	27.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.59000000 0.05000000 NUM

19e3_dcca

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.0	23.9	20.0	20.2
Well 2	19.1	23.3	19.6	19.1
Well 3	20.0	23.3	20.4	19.3
mean Ct	19.4	23.5	20.0	19.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

24.25000000 0.05000000 NUM

191f_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.0	22.6	17.4	25.5
Well 2	17.3	23.1	17.1	26.2
Well 3	17.0	23.0	18.1	25.6
mean Ct	17.1	22.9	17.5	25.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

834d_dcca

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.0	24.7	17.6	20.7
Well 2	17.8	24.5	17.8	20.3
Well 3	16.9	23.7	18.0	19.8
mean Ct	17.2	24.3	17.8	20.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

24.25000000 0.05000000 NUM

6013_63b9

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.7	24.3	20.3	27.4
Well 2	20.7	24.0	20.3	27.8
Well 3	20.7	23.8	20.9	27.3
mean Ct	20.7	24.0	20.5	27.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

13.00000000 0.05000000 NUM

27b9_b624

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.5	21.4	19.3	19.3
Well 2	19.0	22.6	18.5	17.9
Well 3	19.1	22.5	18.8	18.5
mean Ct	18.9	22.2	18.9	18.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

12.13000000 0.05000000 NUM

ef86_2c55

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.7	27.4	21.5	26.2
Well 2	21.6	27.7	21.2	27.3
Well 3	21.7	28.4	21.5	27.1
mean Ct	21.7	27.8	21.4	26.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.52000000 0.05000000 NUM

9d12_9610

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.2	23.6	18.4	22.1
Well 2	18.0	23.9	18.8	21.7
Well 3	17.8	23.2	18.5	21.1
mean Ct	18.0	23.6	18.6	21.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.06000000 0.05000000 NUM

6312_f531

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.1	28.8	19.8	26.1
Well 2	21.2	28.1	20.5	25.9
Well 3	20.4	29.1	19.5	27.0
mean Ct	20.9	28.7	19.9	26.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

2.64000000 0.05000000 NUM

a774_f3b3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.0	24.9	18.6	29.5
Well 2	19.2	25.5	19.5	28.3
Well 3	19.4	26.4	18.6	29.6
mean Ct	19.2	25.6	18.9	29.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

13.93000000 0.05000000 NUM

d71e_3d63

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	16.6	21.5	17.8	23.5
Well 2	16.8	20.8	17.0	24.9
Well 3	18.2	21.5	16.4	24.4
mean Ct	17.2	21.3	17.1	24.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

8.57000000 0.05000000 NUM

615e_43be

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.0	26.0	18.1	25.3
Well 2	18.1	27.0	19.1	25.3
Well 3	18.2	26.4	19.2	25.2
mean Ct	18.4	26.5	18.8	25.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.03000000 0.05000000 NUM

c7c2_43be

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.1	23.3	18.1	21.6
Well 2	17.0	22.4	18.4	22.0
Well 3	17.7	23.0	17.9	21.9
mean Ct	17.6	22.9	18.1	21.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.03000000 0.05000000 NUM

f16f_7419

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.6	24.8	17.9	25.6
Well 2	17.8	25.1	18.1	26.2
Well 3	18.6	24.3	17.7	25.7
mean Ct	18.3	24.7	17.9	25.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

2.83000000 0.05000000 NUM

3381_836a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.3	26.0	20.9	23.0
Well 2	20.9	25.8	21.8	22.8
Well 3	20.6	25.8	22.2	21.7
mean Ct	20.9	25.9	21.6	22.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

17.15000000 0.05000000 NUM

30f1_deff

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.5	26.7	21.4	25.8
Well 2	22.2	26.5	21.4	25.7
Well 3	21.5	27.2	21.8	25.2
mean Ct	21.7	26.8	21.5	25.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.00000000 0.05000000 NUM

2655_1fa9

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.3	27.1	18.9	23.9
Well 2	19.1	26.4	19.6	24.3
Well 3	19.8	27.0	20.2	24.3
mean Ct	19.4	26.8	19.6	24.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.96000000 0.05000000 NUM

8404_deff

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.7	22.0	19.5	22.0
Well 2	19.0	21.9	19.4	21.1
Well 3	19.1	22.6	19.1	21.6
mean Ct	18.9	22.2	19.3	21.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.00000000 0.05000000 NUM

c2c5_836a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.1	22.2	17.6	26.0
Well 2	18.1	21.8	16.7	25.8
Well 3	17.4	23.2	17.1	26.6
mean Ct	17.5	22.4	17.1	26.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

17.15000000 0.05000000 NUM

2e4a_5a7c

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.0	20.8	17.0	24.6
Well 2	18.0	22.1	17.1	24.8
Well 3	18.2	21.2	17.6	24.7
mean Ct	18.1	21.4	17.2	24.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

18.38000000 0.05000000 NUM

79e2_5927

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.6	23.7	19.7	19.7
Well 2	19.3	23.0	20.2	18.4
Well 3	19.2	23.5	19.8	19.1
mean Ct	19.4	23.4	19.9	19.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

27.86000000 0.05000000 NUM

cd6e_b89a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.8	28.8	20.6	28.3
Well 2	21.9	28.6	20.9	29.1
Well 3	21.9	29.6	21.0	29.1
mean Ct	21.9	29.0	20.8	28.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

1.87000000 0.05000000 NUM

b7f6_563f

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.6	27.7	21.4	25.5
Well 2	20.6	28.7	22.0	26.5
Well 3	20.8	28.6	21.7	26.4
mean Ct	20.7	28.3	21.7	26.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

9.19000000 0.05000000 NUM

90c8_782b

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.5	25.4	17.0	27.3
Well 2	18.0	25.2	17.2	26.9
Well 3	18.6	25.8	17.6	28.1
mean Ct	18.4	25.5	17.3	27.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

8.00000000 0.05000000 NUM

3038_2c55

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.2	25.4	17.7	24.8
Well 2	18.0	25.1	18.3	24.0
Well 3	18.0	25.8	18.6	25.1
mean Ct	18.4	25.4	18.2	24.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.52000000 0.05000000 NUM

861d_f704

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.6	20.8	17.6	25.7
Well 2	17.8	20.5	17.8	24.3
Well 3	18.7	21.4	17.6	25.3
mean Ct	18.0	20.9	17.7	25.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

22.63000000 0.05000000 NUM

9729_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.4	23.2	17.7	23.6
Well 2	17.4	22.1	16.4	25.1
Well 3	18.2	22.0	17.1	24.2
mean Ct	17.7	22.4	17.1	24.3
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

29f6_2c55

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.4	24.5	18.2	24.9
Well 2	18.3	24.3	18.1	24.9
Well 3	17.5	24.7	17.9	25.4
mean Ct	18.1	24.5	18.1	25.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.52000000 0.05000000 NUM

a0e1_a839

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.7	24.8	18.5	26.3
Well 2	18.5	24.2	17.2	27.5
Well 3	18.6	24.6	18.6	27.2
mean Ct	18.3	24.5	18.1	27.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.50000000 0.05000000 NUM

8834_0f0a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.5	21.4	17.1	19.8
Well 2	18.5	20.7	17.5	19.5
Well 3	17.3	21.0	16.6	19.3
mean Ct	18.1	21.0	17.1	19.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.41000000 0.05000000 NUM

a947_b624

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.7	22.3	17.3	26.1
Well 2	18.5	23.3	17.6	26.3
Well 3	18.5	23.1	18.8	26.1
mean Ct	18.2	22.9	17.9	26.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

12.13000000 0.05000000 NUM

c2e3_cf70

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.1	28.5	19.7	27.7
Well 2	20.7	28.5	20.4	27.1
Well 3	20.5	27.9	20.5	27.5
mean Ct	20.4	28.3	20.2	27.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.62000000 0.05000000 NUM

5c33_a809

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.2	23.4	19.0	18.6
Well 2	19.3	22.4	19.3	18.4
Well 3	18.9	22.9	19.3	18.3
mean Ct	18.8	22.9	19.2	18.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

29.86000000 0.05000000 NUM

fb73_bcb3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	16.8	23.6	17.1	21.2
Well 2	17.6	24.1	16.4	22.2
Well 3	17.3	23.7	17.7	21.6
mean Ct	17.2	23.8	17.1	21.7
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.00000000 0.05000000 NUM

98da_c3c8

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.7	23.6	19.4	21.3
Well 2	19.4	24.1	18.7	20.3
Well 3	19.8	23.1	19.1	20.9
mean Ct	19.6	23.6	19.1	20.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.92000000 0.05000000 NUM

85d4_3b29

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.8	22.1	18.8	20.6
Well 2	18.5	22.3	18.5	20.8
Well 3	19.0	22.7	18.3	20.3
mean Ct	18.4	22.4	18.5	20.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

3.73000000 0.05000000 NUM

29eb_8b24

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.5	25.2	17.2	19.9
Well 2	17.8	25.1	16.7	20.6
Well 3	17.3	24.6	17.1	21.4
mean Ct	17.5	25.0	17.0	20.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

14.93000000 0.05000000 NUM

c684_7419

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.9	21.8	18.8	21.4
Well 2	18.7	22.5	20.0	21.5
Well 3	18.4	22.9	19.1	21.6
mean Ct	18.7	22.4	19.3	21.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

2.83000000 0.05000000 NUM

cf35_8da6

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.0	26.2	17.7	26.3
Well 2	18.5	25.7	17.2	25.9
Well 3	18.0	26.3	17.5	26.3
mean Ct	18.5	26.1	17.5	26.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.14000000 0.05000000 NUM

9356_dcca

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.2	25.4	21.0	21.3
Well 2	19.9	25.6	19.8	21.2
Well 3	20.5	26.3	20.2	22.3
mean Ct	19.9	25.8	20.3	21.6
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

24.25000000 0.05000000 NUM

01c1_6160

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.2	25.3	18.2	27.8
Well 2	16.9	25.2	17.0	28.1
Well 3	17.0	25.6	18.1	27.8
mean Ct	17.4	25.4	17.8	27.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.29000000 0.05000000 NUM

858d_0f0a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.9	25.3	19.5	26.0
Well 2	19.8	25.1	19.1	26.5
Well 3	18.8	25.1	19.9	25.4
mean Ct	19.2	25.2	19.5	26.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 4% of the correct number to be correct.

1.41000000 0.05000000 NUM

5f5c_a839

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.1	27.5	19.3	24.6
Well 2	20.5	27.9	19.5	24.2
Well 3	19.4	27.8	18.8	23.7
mean Ct	20.0	27.7	19.2	24.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

6.50000000 0.05000000 NUM

25fd_a3c3

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	18.7	25.9	17.9	28.8
Well 2	17.6	24.7	18.0	29.3
Well 3	18.4	25.8	19.0	29.1
mean Ct	18.2	25.5	18.3	29.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

11.31000000 0.05000000 NUM

ed73_8da6

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.8	26.9	20.5	25.3
Well 2	21.3	26.4	21.6	25.4
Well 3	20.5	26.1	21.0	25.9
mean Ct	20.9	26.5	21.0	25.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.14000000 0.05000000 NUM

fe7e_8da6

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	19.7	25.4	19.8	24.4
Well 2	19.8	25.0	19.3	24.2
Well 3	18.9	24.5	19.8	23.5
mean Ct	19.5	25.0	19.6	24.0
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.14000000 0.05000000 NUM

5f7c_6160

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.8	23.8	20.3	25.1
Well 2	21.0	24.2	19.9	25.6
Well 3	20.7	24.0	20.0	25.5
mean Ct	20.8	24.0	20.1	25.4
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.29000000 0.05000000 NUM

33ad_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.7	28.1	20.0	25.8
Well 2	20.7	28.1	21.2	25.7
Well 3	19.7	27.4	21.3	26.0
mean Ct	20.4	27.9	20.8	25.8
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

da0a_8da6

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.0	24.2	20.4	25.0
Well 2	20.7	24.3	20.8	25.0
Well 3	21.5	24.1	20.9	24.7
mean Ct	21.1	24.2	20.7	24.9
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.14000000 0.05000000 NUM

8a4d_715d

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.6	28.5	20.5	30.6
Well 2	20.8	28.3	20.6	30.8
Well 3	19.8	28.7	20.4	31.8
mean Ct	20.4	28.5	20.5	31.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

5.66000000 0.05000000 NUM

4c06_4e2a

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	20.9	28.4	21.3	29.4
Well 2	21.8	28.4	21.2	29.9
Well 3	21.9	28.4	21.4	29.2
mean Ct	21.5	28.4	21.3	29.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 3% of the correct number to be correct.

2.46000000 0.05000000 NUM

de4c_c3c8

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.1	23.7	21.0	21.4
Well 2	20.6	23.4	20.5	21.2
Well 3	20.7	24.6	20.7	21.9
mean Ct	20.8	23.9	20.7	21.5
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 2% of the correct number to be correct.

4.92000000 0.05000000 NUM

70ca_ec81

	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	21.5	25.5	20.2	28.9
Well 2	21.5	25.1	21.1	29.1
Well 3	22.0	24.7	20.8	29.2
mean Ct	21.7	25.1	20.7	29.1
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.

Note: answers need to be within 1% of the correct number to be correct.

32.00000000 0.05000000