MC 32e3_2a51
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | G | , | A | C | G | , | G | A | G | , | C | T | A | , | T | G | G | – | | – | A | G | C | , | T | G | C | , | C | T | C | , | G | A | T | , | A | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC d2ee_156d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | A | , | C | T | A | , | G | G | T | , | A | G | T | , | C | C | A | – | | – | C | T | T | , | G | A | T | , | C | C | A | , | T | C | A | , | G | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC fb45_df81
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | A | , | G | C | T | , | C | G | T | , | G | G | C | , | A | A | T | – | | – | G | G | T | , | C | G | A | , | G | C | A | , | C | C | G | , | T | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 46b3_4256
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | C | T | A | , | T | G | T | , | A | T | C | , | A | C | G | – | | – | T | C | G | , | G | A | T | , | A | C | A | , | T | A | G | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 9e44_8be6
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | G | C | A | , | C | T | C | , | C | A | G | , | T | A | G | – | | – | T | A | C | , | C | G | T | , | G | A | G | , | G | T | C | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC faf5_b51b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | G | A | G | , | T | A | G | , | C | T | A | , | T | G | A | – | | – | G | T | A | , | C | T | C | , | A | T | C | , | G | A | T | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ff03_42fe
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | C | , | G | T | C | , | T | G | A | , | G | G | C | , | T | A | C | – | | – | T | G | G | , | C | A | G | , | A | C | T | , | C | C | G | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 1fbf_169a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | G | T | G | , | A | C | T | , | T | A | C | , | G | G | T | – | | – | G | T | A | , | C | A | C | , | T | G | A | , | A | T | G | , | C | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2574_3b43
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | C | , | T | A | G | , | T | C | T | , | G | C | G | , | T | A | A | – | | – | G | G | G | , | A | T | C | , | A | G | A | , | C | G | C | , | A | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC b94c_c5d3
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | G | G | C | , | T | G | T | , | G | C | A | , | C | T | C | – | | – | T | C | A | , | C | C | G | , | A | C | A | , | C | G | T | , | G | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5bab_b42f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | T | G | G | , | T | A | A | , | C | T | T | , | A | G | G | – | | – | T | C | G | , | A | C | C | , | A | T | T | , | G | A | A | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC bc4e_3e28
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | C | , | T | G | G | , | C | T | C | , | T | G | G | , | A | C | T | – | | – | G | T | G | , | A | C | C | , | G | A | G | , | A | C | C | , | T | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 08bd_d413
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | G | , | T | G | C | , | A | C | T | , | G | T | A | , | C | G | C | – | | – | T | T | C | , | A | C | G | , | T | G | A | , | C | A | T | , | G | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC f0d2_eef8
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | G | C | T | , | A | G | C | , | T | G | G | , | A | C | A | – | | – | C | T | G | , | C | G | A | , | T | C | G | , | A | C | C | , | T | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 80ff_31a0
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | A | , | G | T | G | , | A | C | T | , | A | A | C | , | G | G | T | – | | – | G | G | T | , | C | A | C | , | T | G | A | , | T | T | G | , | C | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c1bb_fce7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | C | , | C | T | A | , | A | C | T | , | A | C | C | , | T | A | G | – | | – | A | C | G | , | G | A | T | , | T | G | A | , | T | G | G | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC f54a_2e24
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | C | , | A | A | G | , | T | T | G | , | G | A | T | , | C | A | A | – | | – | G | A | G | , | T | T | C | , | A | A | C | , | C | T | A | , | G | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 3972_a42d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | C | , | G | C | T | , | G | G | A | , | T | C | G | , | A | T | T | – | | – | G | T | G | , | C | G | A | , | C | C | T | , | A | G | C | , | T | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC c1d2_c6e8
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | A | , | T | C | G | , | C | T | C | , | T | C | T | , | G | C | A | – | | – | C | T | T | , | A | G | C | , | G | A | G | , | A | G | A | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a974_3764
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | C | , | T | G | A | , | T | T | C | , | G | A | T | , | A | C | G | – | | – | T | G | G | , | A | C | T | , | A | A | G | , | C | T | A | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 375a_802b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | G | , | C | T | C | , | C | C | T | , | T | C | C | , | A | G | C | – | | – | A | T | C | , | G | A | G | , | G | G | A | , | A | G | G | , | T | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ea16_5054
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | G | , | C | T | G | , | G | A | G | , | T | C | A | , | G | A | G | – | | – | T | C | C | , | G | A | C | , | C | T | C | , | A | G | T | , | C | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC e253_4b2d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | C | , | A | T | G | , | G | A | T | , | A | G | C | , | T | T | G | – | | – | T | T | G | , | T | A | C | , | C | T | A | , | T | C | G | , | A | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0386_1578
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | A | , | C | T | A | , | G | A | G | , | C | T | T | , | G | C | A | – | | – | C | A | T | , | G | A | T | , | C | T | C | , | G | A | A | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 7fb6_29dc
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | A | , | G | T | C | , | C | G | T | , | C | G | A | , | C | T | A | – | | – | G | C | T | , | C | A | G | , | G | C | A | , | G | C | T | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 60dd_8120
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | A | , | A | G | C | , | G | T | G | , | T | G | C | , | A | A | G | – | | – | A | G | T | , | T | C | G | , | C | A | C | , | A | C | G | , | T | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2230_732b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | G | , | T | C | G | , | G | T | T | , | C | A | C | , | T | G | C | – | | – | T | C | C | , | A | G | C | , | C | A | A | , | G | T | G | , | A | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 9c91_9761
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | G | T | C | , | C | G | A | , | A | A | G | , | C | T | C | – | | – | T | C | G | , | C | A | G | , | G | C | T | , | T | T | C | , | G | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 5b73_7539
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | T | G | C | , | T | T | G | , | A | C | G | , | T | A | A | – | | – | G | A | T | , | A | C | G | , | A | A | C | , | T | G | C | , | A | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 4442_9446
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | A | , | A | C | C | , | A | T | G | , | T | C | A | , | C | G | C | – | | – | A | C | T | , | T | G | G | , | T | A | C | , | A | G | T | , | G | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC a522_5690
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | G | A | C | , | G | G | A | , | T | A | C | , | G | T | C | – | | – | T | C | A | , | C | T | G | , | C | C | T | , | A | T | G | , | C | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 61c8_020c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | A | , | G | C | A | , | T | G | A | , | C | G | A | , | A | T | C | – | | – | A | T | T | , | C | G | T | , | A | C | T | , | G | C | T | , | T | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC f21b_acd8
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | C | , | G | A | T | , | T | C | A | , | T | C | C | , | G | A | T | – | | – | C | A | G | , | C | T | A | , | A | G | T | , | A | G | G | , | C | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC fb97_d30e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | C | A | G | , | C | A | C | , | C | G | A | , | G | T | G | – | | – | T | C | A | , | G | T | C | , | G | T | G | , | G | C | T | , | C | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3b8b_40bc
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | A | G | T | , | G | C | A | , | G | C | C | , | A | T | A | – | | – | G | A | T | , | T | C | A | , | C | G | T | , | C | G | G | , | T | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 8ae8_0255
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | A | , | C | G | T | , | T | A | G | , | A | T | C | , | A | C | G | – | | – | A | C | T | , | G | C | A | , | A | T | C | , | T | A | G | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 6acb_5d2e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | T | , | G | G | G | , | T | C | G | , | C | A | T | , | G | A | C | – | | – | T | G | A | , | C | C | C | , | A | G | C | , | G | T | A | , | C | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5a77_2e5d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | G | A | T | , | T | C | A | , | T | C | T | , | T | A | G | – | | – | T | G | C | , | C | T | A | , | A | G | T | , | A | G | A | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 80ad_f6d1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | G | C | A | , | G | T | C | , | T | C | A | , | C | G | T | – | | – | C | T | A | , | C | G | T | , | C | A | G | , | A | G | T | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7625_040a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | T | , | T | G | A | , | G | T | T | , | A | G | T | , | C | G | T | – | | – | C | G | A | , | A | C | T | , | C | A | A | , | T | C | A | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC cb1e_2a1d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | A | , | C | A | C | , | T | T | G | , | T | C | G | , | A | C | T | – | | – | C | A | T | , | G | T | G | , | A | A | C | , | A | G | C | , | T | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0d14_7ef8
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | A | , | C | T | G | , | A | A | C | , | T | G | C | , | G | A | G | – | | – | T | T | T | , | G | A | C | , | T | T | G | , | A | C | G | , | C | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC d2e8_60ff
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | G | C | A | , | A | G | G | , | C | A | A | , | G | A | T | – | | – | C | T | A | , | C | G | T | , | T | C | C | , | G | T | T | , | C | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 8109_1ee5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | T | T | A | , | C | T | T | , | C | A | C | , | T | G | A | – | | – | G | A | C | , | A | A | T | , | G | A | A | , | G | T | G | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC bf69_2ddb
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | C | , | T | T | G | , | A | C | T | , | G | T | C | , | A | A | C | – | | – | A | T | G | , | A | A | C | , | T | G | A | , | C | A | G | , | T | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC cf37_846d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | C | T | A | , | T | G | C | , | G | T | C | , | T | T | A | – | | – | C | T | G | , | G | A | T | , | A | C | G | , | C | A | G | , | A | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3a7b_827c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | T | , | C | A | T | , | A | G | G | , | G | T | C | , | G | A | A | – | | – | G | C | A | , | G | T | A | , | T | C | C | , | C | A | G | , | C | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5302_a0a4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | G | , | A | C | T | , | G | C | C | , | T | A | T | , | G | C | G | – | | – | A | A | C | , | T | G | A | , | C | G | G | , | A | T | A | , | C | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 6f2d_b659
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | C | T | A | , | T | C | T | , | T | C | C | , | A | G | C | – | | – | T | C | A | , | G | A | T | , | A | G | A | , | A | G | G | , | T | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC e6ac_c07e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | A | , | T | G | C | , | G | A | C | , | T | G | C | , | T | A | C | – | | – | T | C | T | , | A | C | G | , | C | T | G | , | A | C | G | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC dd56_4555
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | T | , | G | A | C | , | T | G | T | , | C | T | A | , | G | T | A | – | | – | C | G | A | , | C | T | G | , | A | C | A | , | G | A | T | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2410_2e83
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | T | , | G | G | A | , | A | A | G | , | T | C | G | , | T | C | A | – | | – | G | G | A | , | C | C | T | , | T | T | C | , | A | G | C | , | A | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 6839_07fb
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | G | , | C | A | T | , | T | G | T | , | C | T | G | , | T | G | A | – | | – | C | T | C | , | G | T | A | , | A | C | A | , | G | A | C | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ba62_bb07
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | C | , | T | A | G | , | C | A | G | , | A | C | T | , | A | G | G | – | | – | T | G | G | , | A | T | C | , | G | T | C | , | T | G | A | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 51ec_a2c7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | T | , | A | C | T | , | G | T | C | , | A | C | A | , | G | T | A | – | | – | G | C | A | , | T | G | A | , | C | A | G | , | T | G | T | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC e323_baa1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | T | A | C | , | T | A | G | , | A | C | T | , | C | G | T | – | | – | C | T | G | , | A | T | G | , | A | T | C | , | T | G | A | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4dc5_d83a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | T | C | G | , | T | G | G | , | A | G | T | , | C | T | A | – | | – | G | T | A | , | A | G | C | , | A | C | C | , | T | C | A | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC e21e_d3bb
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | A | , | G | T | T | , | C | A | G | , | C | G | A | , | T | G | A | – | | – | G | C | T | , | C | A | A | , | G | T | C | , | G | C | T | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 1466_4306
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | C | , | G | T | G | , | G | T | A | , | C | A | T | , | C | C | G | – | | – | A | T | G | , | C | A | C | , | C | A | T | , | G | T | A | , | G | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC b80e_1429
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | C | , | T | A | A | , | G | T | C | , | A | G | C | , | T | C | T | – | | – | C | A | G | , | A | T | T | , | C | A | G | , | T | C | G | , | A | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 44ba_a393
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | C | A | G | , | T | G | C | , | C | A | T | , | G | A | C | – | | – | T | C | A | , | G | T | C | , | A | C | G | , | G | T | A | , | C | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b463_41a1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | G | , | A | T | C | , | G | C | A | , | T | G | G | , | A | C | C | – | | – | T | C | C | , | T | A | G | , | C | G | T | , | A | C | C | , | T | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC e3ed_a396
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | G | T | G | , | A | G | A | , | G | A | A | , | C | T | A | – | | – | G | A | T | , | C | A | C | , | T | C | T | , | C | T | T | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b518_c627
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | A | , | C | G | G | , | A | A | G | , | T | A | C | , | A | G | G | – | | – | A | G | T | , | G | C | C | , | T | T | C | , | A | T | G | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 0bba_198c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | G | , | C | A | T | , | A | G | A | , | T | C | G | , | A | G | G | – | | – | T | C | C | , | G | T | A | , | T | C | T | , | A | G | C | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 4b54_b0d6
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | T | , | G | C | A | , | G | A | A | , | G | T | A | , | C | C | A | – | | – | G | A | A | , | C | G | T | , | C | T | T | , | C | A | T | , | G | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC a97a_fd95
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | T | , | A | T | C | , | A | A | T | , | G | A | T | , | G | G | C | – | | – | A | C | A | , | T | A | G | , | T | T | A | , | C | T | A | , | C | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 32ce_3ea6
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | T | C | G | , | A | A | C | , | G | A | T | , | T | C | T | – | | – | C | T | G | , | A | G | C | , | T | T | G | , | C | T | A | , | A | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC acc8_e203
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | A | G | C | , | T | T | A | , | G | T | T | , | A | C | G | – | | – | T | A | C | , | T | C | G | , | A | A | T | , | C | A | A | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 33bd_06bc
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | C | , | G | T | T | , | C | T | C | , | A | G | T | , | C | C | A | – | | – | G | T | G | , | C | A | A | , | G | A | G | , | T | C | A | , | G | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 5cd9_0fb7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | A | , | G | C | C | , | T | T | C | , | T | A | A | , | C | T | G | – | | – | A | C | T | , | C | G | G | , | A | A | G | , | A | T | T | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 4fdd_a683
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | G | , | T | A | C | , | T | A | C | , | C | T | G | , | G | C | A | – | | – | C | C | C | , | A | T | G | , | A | T | G | , | G | A | C | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2ffd_9fce
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | C | , | T | A | G | , | C | G | A | , | A | A | G | , | A | C | T | – | | – | G | C | G | , | A | T | C | , | G | C | T | , | T | T | C | , | T | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3349_2835
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | T | , | A | G | C | , | G | A | G | , | T | C | G | , | C | A | C | – | | – | T | G | A | , | T | C | G | , | C | T | C | , | A | G | C | , | G | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC b856_7e21
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | T | , | G | C | A | , | A | C | A | , | G | C | T | , | G | A | T | – | | – | G | A | A | , | C | G | T | , | T | G | T | , | C | G | A | , | C | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 26ef_3890
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | C | C | G | , | A | T | T | , | C | T | G | , | G | C | A | – | | – | C | T | A | , | G | G | C | , | T | A | A | , | G | A | C | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC b741_400d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | G | A | C | , | C | G | A | , | G | T | G | , | C | A | T | – | | – | C | T | A | , | C | T | G | , | G | C | T | , | C | A | C | , | G | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 04cd_0f39
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | C | , | G | A | A | , | C | C | T | , | G | A | A | , | C | T | C | – | | – | A | C | G | , | C | T | T | , | G | G | A | , | C | T | T | , | G | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ac64_a4e1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | A | G | T | , | T | C | A | , | A | A | G | , | T | C | C | – | | – | T | C | G | , | T | C | A | , | A | G | T | , | T | T | C | , | A | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC f290_6e2a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | A | , | C | G | T | , | A | C | T | , | G | A | G | , | C | T | A | – | | – | G | C | T | , | G | C | A | , | T | G | A | , | C | T | C | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5141_70a9
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | T | C | T | , | A | C | A | , | A | C | A | , | T | A | G | – | | – | T | C | G | , | A | G | A | , | T | G | T | , | T | G | T | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 722c_0e29
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | T | , | A | G | T | , | G | T | G | , | A | C | A | , | G | G | T | – | | – | G | A | A | , | T | C | A | , | C | A | C | , | T | G | T | , | C | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC f76f_4f4e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | G | , | T | A | C | , | T | T | T | , | G | T | A | , | G | C | T | – | | – | C | G | C | , | A | T | G | , | A | A | A | , | C | A | T | , | C | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 002e_78b5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | C | , | A | A | A | , | G | C | C | , | A | G | C | , | T | C | C | – | | – | A | C | G | , | T | T | T | , | C | G | G | , | T | C | G | , | A | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 1056_0cf4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | T | , | C | A | G | , | T | C | A | , | C | G | T | , | G | A | A | – | | – | C | A | A | , | G | T | C | , | A | G | T | , | G | C | A | , | C | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 5b79_0e13
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | T | G | C | , | G | T | G | , | C | A | G | , | T | T | G | – | | – | T | A | G | , | A | C | G | , | C | A | C | , | G | T | C | , | A | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC d7b6_e562
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | A | C | T | , | C | A | G | , | G | T | C | , | C | T | A | – | | – | G | A | C | , | T | G | A | , | G | T | C | , | C | A | G | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC e927_8d35
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | A | , | T | G | T | , | T | C | G | , | C | T | G | , | T | G | A | – | | – | G | G | T | , | A | C | A | , | A | G | C | , | G | A | C | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4151_59d5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | C | , | T | A | G | , | G | G | A | , | A | T | G | , | G | C | A | – | | – | G | T | G | , | A | T | C | , | C | C | T | , | T | A | C | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 88d1_8f6d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | T | , | G | A | T | , | T | G | T | , | G | C | T | , | A | T | G | – | | – | T | G | A | , | C | T | A | , | A | C | A | , | C | G | A | , | T | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 87e5_8017
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | T | , | C | G | A | , | G | T | C | , | T | A | G | , | T | C | A | – | | – | C | C | A | , | G | C | T | , | C | A | G | , | A | T | C | , | A | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f41f_4b1e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | G | , | C | A | G | , | A | G | G | , | G | A | G | , | T | C | C | – | | – | A | A | C | , | G | T | C | , | T | C | C | , | C | T | C | , | A | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4c89_4cb5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | G | , | T | A | A | , | C | A | T | , | G | T | A | , | C | G | A | – | | – | G | C | C | , | A | T | T | , | G | T | A | , | C | A | T | , | G | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 6e38_a6bd
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | A | C | A | , | T | T | C | , | T | T | G | , | A | A | C | – | | – | T | A | C | , | T | G | T | , | A | A | G | , | A | A | C | , | T | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 50b5_ff08
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | T | G | C | , | A | C | A | , | A | G | T | , | C | C | G | – | | – | T | A | C | , | A | C | G | , | T | G | T | , | T | C | A | , | G | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ac48_6ab4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | G | C | G | , | A | T | C | , | T | A | C | , | A | G | T | – | | – | G | T | A | , | C | G | C | , | T | A | G | , | A | T | G | , | T | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f75d_608e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | G | , | A | T | G | , | C | T | G | , | T | G | C | , | C | A | T | – | | – | G | T | C | , | T | A | C | , | G | A | C | , | A | C | G | , | G | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 460e_6f42
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | G | , | T | C | T | , | G | A | C | , | G | C | T | , | A | T | G | – | | – | A | T | C | , | A | G | A | , | C | T | G | , | C | G | A | , | T | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 1d92_b2d2
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | A | , | G | T | G | , | G | T | C | , | A | T | G | , | C | C | A | – | | – | G | C | T | , | C | A | C | , | C | A | G | , | T | A | C | , | G | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 6f1d_21fd
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | A | , | G | C | T | , | C | C | A | , | T | A | C | , | A | G | G | – | | – | A | A | T | , | C | G | A | , | G | G | T | , | A | T | G | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b9b9_f78d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | A | , | C | G | C | , | A | G | T | , | G | A | C | , | A | T | A | – | | – | C | A | T | , | G | C | G | , | T | C | A | , | C | T | G | , | T | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5514_a142
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | C | , | G | C | T | , | C | T | C | , | G | C | T | , | T | A | G | – | | – | A | T | G | , | C | G | A | , | G | A | G | , | C | G | A | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0683_16f6
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | A | T | G | , | C | T | A | , | G | T | G | , | A | G | C | – | | – | T | A | G | , | T | A | C | , | G | A | T | , | C | A | C | , | T | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 2e81_66c5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | A | , | T | A | C | , | T | T | C | , | C | A | T | , | T | G | G | – | | – | T | C | T | , | A | T | G | , | A | A | G | , | G | T | A | , | A | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC df44_a785
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | C | , | A | G | T | , | G | G | A | , | T | G | C | , | A | G | C | – | | – | A | G | G | , | T | C | A | , | C | C | T | , | A | C | G | , | T | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3877_a062
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | G | , | A | A | A | , | C | A | C | , | G | A | T | , | C | G | C | – | | – | A | G | C | , | T | T | T | , | G | T | G | , | C | T | A | , | G | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC e400_1c75
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | T | , | C | A | G | , | A | G | G | , | G | A | G | , | T | T | C | – | | – | A | T | A | , | G | T | C | , | T | C | C | , | C | T | C | , | A | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC a4a7_2701
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | C | , | A | T | G | , | A | C | A | , | C | A | G | , | T | G | T | – | | – | G | G | G | , | T | A | C | , | T | G | T | , | G | T | C | , | A | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC fcb5_4ddc
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | C | T | C | , | C | A | G | , | A | A | C | , | G | G | T | – | | – | C | T | A | , | G | A | G | , | G | T | C | , | T | T | G | , | C | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 3c2d_3ad5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | A | , | G | C | T | , | A | G | A | , | T | T | C | , | A | G | C | – | | – | A | G | T | , | C | G | A | , | T | C | T | , | A | A | G | , | T | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 92ff_ed50
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | T | , | G | C | C | , | T | C | C | , | T | T | C | , | T | A | G | – | | – | A | T | A | , | C | G | G | , | A | G | G | , | A | A | G | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 99b7_0f60
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | G | , | T | A | G | , | C | G | A | , | A | C | C | , | G | T | A | – | | – | G | T | C | , | A | T | C | , | G | C | T | , | T | G | G | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC e2a3_8607
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | T | , | C | G | C | , | T | A | G | , | T | T | G | , | A | G | C | – | | – | T | A | A | , | G | C | G | , | A | T | C | , | A | A | C | , | T | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 0b25_1a17
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | A | , | T | C | A | , | G | T | G | , | C | G | T | , | A | G | A | – | | – | G | C | T | , | A | G | T | , | C | A | C | , | G | C | A | , | T | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7c6e_0b63
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | G | T | C | , | C | A | C | , | T | A | A | , | G | C | G | – | | – | T | A | G | , | C | A | G | , | G | T | G | , | A | T | T | , | C | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2c05_abc9
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | C | , | A | A | G | , | C | T | T | , | G | T | T | , | C | A | A | – | | – | G | A | G | , | T | T | C | , | G | A | A | , | C | A | A | , | G | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 5fe9_f1b5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | T | , | A | G | C | , | A | C | G | , | C | A | A | , | G | A | T | – | | – | G | A | A | , | T | C | G | , | T | G | C | , | G | T | T | , | C | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 8c81_3ddd
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | A | , | C | C | A | , | A | G | G | , | G | A | A | , | C | T | G | – | | – | A | C | T | , | G | G | T | , | T | C | C | , | C | T | T | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 120a_a973
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | C | A | A | , | C | A | C | , | A | C | A | , | C | G | T | – | | – | G | A | C | , | G | T | T | , | G | T | G | , | T | G | T | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f1dd_d9ba
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | C | A | A | , | C | T | C | , | G | A | C | , | G | C | T | – | | – | G | A | C | , | G | T | T | , | G | A | G | , | C | T | G | , | C | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 3faa_0abc
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | G | A | C | , | T | G | A | , | G | T | G | , | A | C | T | – | | – | C | T | A | , | C | T | G | , | A | C | T | , | C | A | C | , | T | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 1d28_f138
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | C | , | A | G | G | , | T | T | A | , | C | G | A | , | C | A | T | – | | – | G | A | G | , | T | C | C | , | A | A | T | , | G | C | T | , | G | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 63e4_1776
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | C | , | C | G | T | , | A | T | G | , | A | A | G | , | T | A | C | – | | – | T | T | G | , | G | C | A | , | T | A | C | , | T | T | C | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 86a9_e69e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | C | , | A | A | G | , | T | C | C | , | A | G | T | , | T | A | C | – | | – | A | G | G | , | T | T | C | , | A | G | G | , | T | C | A | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 9e1e_f788
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | G | , | T | T | C | , | T | T | G | , | T | C | T | , | A | C | G | – | | – | T | T | C | , | A | A | G | , | A | A | C | , | A | G | A | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC ef67_7906
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | T | , | G | C | A | , | C | C | A | , | A | G | A | , | C | T | A | – | | – | G | G | A | , | C | G | T | , | G | G | T | , | T | C | T | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC faa8_ee1b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | A | , | G | C | T | , | T | G | A | , | G | A | C | , | A | T | G | – | | – | T | C | T | , | C | G | A | , | A | C | T | , | C | T | G | , | T | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC aa7e_779c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | C | , | G | C | T | , | G | C | T | , | A | G | C | , | T | G | C | – | | – | T | T | G | , | C | G | A | , | C | G | A | , | T | C | G | , | A | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC d8b8_9c7b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | C | , | A | G | A | , | G | T | C | , | G | C | T | , | G | C | A | – | | – | G | A | G | , | T | C | T | , | C | A | G | , | C | G | A | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 9bdd_f149
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | G | , | T | G | T | , | C | C | T | , | A | T | G | , | T | C | A | – | | – | G | T | C | , | A | C | A | , | G | G | A | , | T | A | C | , | A | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a092_9619
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | G | , | C | A | T | , | G | C | T | , | G | T | G | , | A | C | T | – | | – | C | T | C | , | G | T | A | , | C | G | A | , | C | A | C | , | T | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 1628_8093
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | A | , | C | G | T | , | C | G | T | , | C | A | T | , | G | C | A | – | | – | C | C | T | , | G | C | A | , | G | C | A | , | G | T | A | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC d5e2_9174
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | A | , | A | T | G | , | T | G | T | , | A | G | T | , | C | A | C | – | | – | T | G | T | , | T | A | C | , | A | C | A | , | T | C | A | , | G | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 4a11_6065
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | A | , | G | T | C | , | C | A | G | , | G | T | A | , | A | C | G | – | | – | A | A | T | , | C | A | G | , | G | T | C | , | C | A | T | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 27df_1b62
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | C | , | A | G | T | , | A | G | T | , | T | C | A | , | T | A | G | – | | – | A | T | G | , | T | C | A | , | T | C | A | , | A | G | T | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC b4de_f10d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | G | , | T | C | G | , | T | T | A | , | G | A | T | , | C | A | T | – | | – | G | T | C | , | A | G | C | , | A | A | T | , | C | T | A | , | G | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC b694_58ff
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | C | , | T | A | T | , | C | C | A | , | A | G | C | , | A | A | T | – | | – | G | C | G | , | A | T | A | , | G | G | T | , | T | C | G | , | T | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC da80_5800
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | C | , | A | G | A | , | A | G | T | , | A | G | G | , | C | T | A | – | | – | G | A | G | , | T | C | T | , | T | C | A | , | T | C | C | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ebdc_38d0
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | A | , | T | G | A | , | G | G | A | , | C | G | T | , | C | G | A | – | | – | G | C | T | , | A | C | T | , | C | C | T | , | G | C | A | , | G | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5ceb_be85
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | T | G | C | , | A | T | G | , | A | A | T | , | C | G | A | – | | – | C | T | A | , | A | C | G | , | T | A | C | , | T | T | A | , | G | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC e84d_2bf4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | C | , | T | G | A | , | G | A | A | , | T | C | A | , | C | C | G | – | | – | A | A | G | , | A | C | T | , | C | T | T | , | A | G | T | , | G | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 364f_4c3b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | A | , | A | T | G | , | T | G | T | , | G | C | G | , | T | A | T | – | | – | G | C | T | , | T | A | C | , | A | C | A | , | C | G | C | , | A | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6a7a_a111
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | T | , | G | A | C | , | G | T | G | , | A | T | G | , | C | C | T | – | | – | G | G | A | , | C | T | G | , | C | A | C | , | T | A | C | , | G | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 22bd_6f8e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | T | , | G | G | A | , | G | A | C | , | T | A | C | , | G | G | T | – | | – | G | C | A | , | C | C | T | , | C | T | G | , | A | T | G | , | C | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC e742_53f4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | T | C | C | , | G | T | C | , | G | A | T | , | A | C | G | – | | – | T | C | G | , | A | G | G | , | C | A | G | , | C | T | A | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 92db_8ddf
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | G | , | A | T | G | , | G | G | T | , | C | A | A | , | C | T | G | – | | – | A | G | C | , | T | A | C | , | C | C | A | , | G | T | T | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 8dce_4b71
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | G | C | T | , | A | G | G | , | G | A | C | , | G | T | T | – | | – | G | T | A | , | C | G | A | , | T | C | C | , | C | T | G | , | C | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC f569_2f99
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | A | , | G | T | A | , | G | A | T | , | G | T | C | , | T | T | A | – | | – | G | G | T | , | C | A | T | , | C | T | A | , | C | A | G | , | A | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 0d5e_81b7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | T | T | G | , | T | T | C | , | C | G | T | , | A | A | C | – | | – | T | A | G | , | A | A | C | , | A | A | G | , | G | C | A | , | T | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC eb28_46f0
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | T | , | G | C | A | , | T | C | C | , | A | C | G | , | A | T | G | – | | – | T | G | A | , | C | G | T | , | A | G | G | , | T | G | C | , | T | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC a0af_8880
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | A | , | T | G | T | , | T | T | C | , | G | G | A | , | C | T | A | – | | – | C | G | T | , | A | C | A | , | A | A | G | , | C | C | T | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 39d8_e07b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | T | C | A | , | C | T | C | , | G | T | A | , | C | G | G | – | | – | T | G | C | , | A | G | T | , | G | A | G | , | C | A | T | , | G | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 2937_13c0
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | A | , | C | G | A | , | C | G | C | , | T | C | C | , | A | G | C | – | | – | A | T | T | , | G | C | T | , | G | C | G | , | A | G | G | , | T | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6f14_bede
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | C | , | T | A | G | , | A | C | C | , | G | A | A | , | C | T | T | – | | – | C | G | G | , | A | T | C | , | T | G | G | , | C | T | T | , | G | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 486e_acf8
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | G | , | C | G | T | , | C | T | T | , | C | A | G | , | T | C | A | – | | – | G | T | C | , | G | C | A | , | G | A | A | , | G | T | C | , | A | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC de30_0b95
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | T | C | T | , | C | A | A | , | C | A | T | , | G | A | C | – | | – | T | G | C | , | A | G | A | , | G | T | T | , | G | T | A | , | C | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 17a6_2a5a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | T | , | G | G | A | , | A | C | C | , | C | A | A | , | G | A | T | – | | – | G | C | A | , | C | C | T | , | T | G | G | , | G | T | T | , | C | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1ea5_32ab
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | T | T | C | , | A | C | C | , | C | A | G | , | A | T | G | – | | – | T | A | C | , | A | A | G | , | T | G | G | , | G | T | C | , | T | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 39e4_c4fa
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | G | , | T | C | A | , | G | C | C | , | T | C | A | , | G | G | G | – | | – | T | T | C | , | A | G | T | , | C | G | G | , | A | G | T | , | C | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 06dd_c97a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | C | , | A | G | A | , | G | T | A | , | T | C | A | , | T | C | G | – | | – | A | A | G | , | T | C | T | , | C | A | T | , | A | G | T | , | A | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 24e9_f58d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | T | , | A | A | C | , | T | T | T | , | C | A | G | , | A | A | T | – | | – | C | C | A | , | T | T | G | , | A | A | A | , | G | T | C | , | T | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2a0c_3f2e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | G | , | T | C | A | , | C | A | T | , | G | C | A | , | A | T | C | – | | – | A | A | C | , | A | G | T | , | G | T | A | , | C | G | T | , | T | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a010_9afd
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | T | , | C | A | G | , | G | T | C | , | A | C | G | , | T | A | T | – | | – | C | C | A | , | G | T | C | , | C | A | G | , | T | G | C | , | A | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 25e2_e0f9
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | C | G | C | , | A | A | G | , | T | A | G | , | T | C | A | – | | – | G | T | A | , | G | C | G | , | T | T | C | , | A | T | C | , | A | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC b888_db83
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | C | , | G | A | A | , | C | G | A | , | T | G | C | , | A | G | T | – | | – | C | A | G | , | C | T | T | , | G | C | T | , | A | C | G | , | T | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 3260_135c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | C | , | C | A | A | , | T | G | A | , | C | G | C | , | A | G | T | – | | – | C | A | G | , | G | T | T | , | A | C | T | , | G | C | G | , | T | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 7c3a_725e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | T | , | G | G | A | , | T | A | G | , | C | T | G | , | T | A | A | – | | – | G | G | A | , | C | C | T | , | A | T | C | , | G | A | C | , | A | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC cf01_6914
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | C | T | G | , | G | C | T | , | G | A | C | , | T | A | G | – | | – | T | A | C | , | G | A | C | , | C | G | A | , | C | T | G | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5ed4_f3de
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | G | A | T | , | G | A | C | , | A | C | C | , | T | G | A | – | | – | G | T | A | , | C | T | A | , | C | T | G | , | T | G | G | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 2d01_85d6
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | T | C | C | , | G | T | T | , | C | G | T | , | T | G | A | – | | – | C | T | G | , | A | G | G | , | C | A | A | , | G | C | A | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 1077_ad55
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | A | , | C | T | T | , | C | C | T | , | G | T | A | , | A | C | C | – | | – | T | C | T | , | G | A | A | , | G | G | A | , | C | A | T | , | T | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9cd3_697b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | G | , | T | A | A | , | G | A | A | , | C | G | T | , | G | T | A | – | | – | C | G | C | , | A | T | T | , | C | T | T | , | G | C | A | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC cd46_e0d1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | A | C | G | , | A | G | T | , | A | G | C | , | T | C | G | – | | – | T | A | C | , | T | G | C | , | T | C | A | , | T | C | G | , | A | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC b77e_ab76
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | T | , | C | C | G | , | A | C | T | , | A | C | A | , | T | A | G | – | | – | A | T | A | , | G | G | C | , | T | G | A | , | T | G | T | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a32f_e2d5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | T | , | G | A | C | , | T | A | A | , | G | A | C | , | A | A | T | – | | – | G | G | A | , | C | T | G | , | A | T | T | , | C | T | G | , | T | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 69cc_9a5c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | G | , | A | A | T | , | G | C | C | , | T | G | A | , | G | C | A | – | | – | G | T | C | , | T | T | A | , | C | G | G | , | A | C | T | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC ac9e_b533
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | C | , | G | G | A | , | C | T | T | , | C | T | A | , | A | G | G | – | | – | A | T | G | , | C | C | T | , | G | A | A | , | G | A | T | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC db9f_1d50
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | G | , | T | C | A | , | G | A | C | , | A | T | G | , | A | C | G | – | | – | A | C | C | , | A | G | T | , | C | T | G | , | T | A | C | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 6ffa_5f4e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | G | , | T | C | G | , | A | A | G | , | C | A | T | , | G | A | T | – | | – | G | T | C | , | A | G | C | , | T | T | C | , | G | T | A | , | C | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC ef37_a702
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | T | T | C | , | G | T | C | , | C | A | G | , | T | C | C | – | | – | T | G | C | , | A | A | G | , | C | A | G | , | G | T | C | , | A | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0dc4_ce13
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | A | , | C | T | C | , | G | C | T | , | C | G | C | , | T | A | G | – | | – | T | C | T | , | G | A | G | , | C | G | A | , | G | C | G | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8ec8_e108
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | A | , | G | C | T | , | T | G | A | , | G | C | T | , | G | A | G | – | | – | A | A | T | , | C | G | A | , | A | C | T | , | C | G | A | , | C | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC e02d_5668
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | A | A | G | , | A | T | G | , | T | C | T | , | T | A | G | – | | – | T | A | G | , | T | T | C | , | T | A | C | , | A | G | A | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0470_833c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | A | G | A | , | G | T | A | , | G | C | T | , | C | A | G | – | | – | T | A | G | , | T | C | T | , | C | A | T | , | C | G | A | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC d510_b146
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | T | T | A | , | G | G | A | , | G | A | A | , | G | C | T | – | | – | G | A | C | , | A | A | T | , | C | C | T | , | C | T | T | , | C | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6c05_b3ac
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | A | , | C | G | A | , | A | G | T | , | C | T | A | , | A | G | G | – | | – | A | G | T | , | G | C | T | , | T | C | A | , | G | A | T | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 954e_6f84
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | C | , | A | T | C | , | C | T | G | , | C | T | G | , | C | T | A | – | | – | G | C | G | , | T | A | G | , | G | A | C | , | G | A | C | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6418_a2a5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | G | A | C | , | G | A | C | , | C | G | T | , | G | A | C | – | | – | T | A | G | , | C | T | G | , | C | T | G | , | G | C | A | , | C | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 8b85_d3c1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | G | C | A | , | T | C | A | , | T | A | C | , | A | C | G | – | | – | T | C | A | , | C | G | T | , | A | G | T | , | A | T | G | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 57f3_a3a4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | A | T | T | , | C | T | T | , | C | A | A | , | G | G | T | – | | – | G | A | C | , | T | A | A | , | G | A | A | , | G | T | T | , | C | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 1eaa_e747
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | T | C | G | , | C | T | G | , | A | C | G | , | T | T | T | – | | – | G | A | T | , | A | G | C | , | G | A | C | , | T | G | C | , | A | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 9e7c_3a7f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | G | G | A | , | T | G | C | , | G | T | A | , | T | C | T | – | | – | G | A | C | , | C | C | T | , | A | C | G | , | C | A | T | , | A | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f540_8853
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | T | , | A | G | A | , | G | G | A | , | A | A | G | , | A | C | T | – | | – | G | G | A | , | T | C | T | , | C | C | T | , | T | T | C | , | T | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 6512_30da
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | A | , | C | C | T | , | T | G | C | , | A | T | A | , | C | G | A | – | | – | G | C | T | , | G | G | A | , | A | C | G | , | T | A | T | , | G | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 9df8_b9ff
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | A | G | C | , | A | G | A | , | C | G | T | , | C | A | C | – | | – | T | A | C | , | T | C | G | , | T | C | T | , | G | C | A | , | G | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 06ff_ff18
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | A | C | G | , | T | G | G | , | A | G | C | , | T | A | T | – | | – | C | T | A | , | T | G | C | , | A | C | C | , | T | C | G | , | A | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1cc8_458e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | T | , | C | T | A | , | A | G | T | , | G | T | A | , | C | T | A | – | | – | C | G | A | , | G | A | T | , | T | C | A | , | C | A | T | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 39fd_4cba
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | C | , | A | G | G | , | T | T | C | , | A | G | T | , | G | C | G | – | | – | A | T | G | , | T | C | C | , | A | A | G | , | T | C | A | , | C | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 64f4_e50f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | A | C | A | , | T | G | T | , | C | A | T | , | T | G | A | – | | – | G | A | C | , | T | G | T | , | A | C | A | , | G | T | A | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect