MC 5b31_2e2a
| 5′– | T | C | A | , | A | G | G | – | | 3′– | A | G | T | , | T | C | C | – | | | | – | T | T | G | , | A | C | G | , | A | G | A | , | A | A | G | , | G | A | T | , | T | G | T | , | G | T | T | , | C | A | G | – | | – | A | A | C | , | T | G | C | , | T | C | T | , | T | T | C | , | C | T | A | , | A | C | A | , | C | A | A | , | G | T | C | – | | | | – | C | C | T | , | G | C | A | –3′ | | – | G | G | A | , | C | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAAGG-3′ and 5′-TGCAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5935_2375
| 5′– | A | G | G | , | T | A | G | – | | 3′– | T | C | C | , | A | T | C | – | | | | – | A | G | T | , | G | C | T | , | G | T | G | , | T | C | C | , | T | C | T | , | T | T | C | , | T | G | A | , | C | G | G | – | | – | T | C | A | , | C | G | A | , | C | A | C | , | A | G | G | , | A | G | A | , | A | A | G | , | A | C | T | , | G | C | C | – | | | | – | G | C | C | , | T | T | A | –3′ | | – | C | G | G | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTAG-3′ and 5′-TAAGGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 519b_d85c
| 5′– | A | T | T | , | G | G | C | – | | 3′– | T | A | A | , | C | C | G | – | | | | – | G | C | C | , | A | T | A | , | C | A | C | , | A | C | C | , | G | A | A | , | T | C | A | , | C | T | G | , | T | T | A | – | | – | C | G | G | , | T | A | T | , | G | T | G | , | T | G | G | , | C | T | T | , | A | G | T | , | G | A | C | , | A | A | T | – | | | | – | G | A | T | , | C | A | A | –3′ | | – | C | T | A | , | G | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATTGGC-3′ and 5′-TTGATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7571_38d0
| 5′– | G | G | A | , | C | T | T | – | | 3′– | C | C | T | , | G | A | A | – | | | | – | C | T | A | , | T | G | G | , | T | C | C | , | A | A | A | , | G | C | A | , | A | G | C | , | A | C | T | , | G | G | A | – | | – | G | A | T | , | A | C | C | , | A | G | G | , | T | T | T | , | C | G | T | , | T | C | G | , | T | G | A | , | C | C | T | – | | | | – | A | G | G | , | G | T | C | –3′ | | – | T | C | C | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGACTT-3′ and 5′-GACCCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a7c7_dd94
| 5′– | A | T | G | , | T | T | G | – | | 3′– | T | A | C | , | A | A | C | – | | | | – | A | A | C | , | T | G | T | , | A | T | C | , | G | A | A | , | C | A | C | , | T | A | A | , | G | T | A | , | C | C | G | – | | – | T | T | G | , | A | C | A | , | T | A | G | , | C | T | T | , | G | T | G | , | A | T | T | , | C | A | T | , | G | G | C | – | | | | – | A | T | G | , | T | G | G | –3′ | | – | T | A | C | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGTTG-3′ and 5′-CCACAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 2a80_71ba
| 5′– | C | G | G | , | A | T | T | – | | 3′– | G | C | C | , | T | A | A | – | | | | – | C | C | G | , | A | A | T | , | G | A | G | , | A | A | A | , | G | A | C | , | C | T | T | , | C | A | C | , | G | T | T | – | | – | G | G | C | , | T | T | A | , | C | T | C | , | T | T | T | , | C | T | G | , | G | A | A | , | G | T | G | , | C | A | A | – | | | | – | G | C | C | , | A | T | T | –3′ | | – | C | G | G | , | T | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGGATT-3′ and 5′-AATGGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6881_6fd7
| 5′– | C | A | T | , | G | T | T | – | | 3′– | G | T | A | , | C | A | A | – | | | | – | G | T | C | , | A | G | T | , | G | A | T | , | C | A | G | , | C | C | A | , | C | A | C | , | A | T | T | , | C | G | A | – | | – | C | A | G | , | T | C | A | , | C | T | A | , | G | T | C | , | G | G | T | , | G | T | G | , | T | A | A | , | G | C | T | – | | | | – | C | C | A | , | G | T | T | –3′ | | – | G | G | T | , | C | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATGTT-3′ and 5′-AACTGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 455a_0a69
| 5′– | C | A | T | , | T | C | A | – | | 3′– | G | T | A | , | A | G | T | – | | | | – | T | T | T | , | C | G | A | , | G | G | C | , | T | T | C | , | T | A | C | , | A | C | C | , | T | A | C | , | G | A | G | – | | – | A | A | A | , | G | C | T | , | C | C | G | , | A | A | G | , | A | T | G | , | T | G | G | , | A | T | G | , | C | T | C | – | | | | – | T | T | A | , | G | C | C | –3′ | | – | A | A | T | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATTCA-3′ and 5′-GGCTAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 4577_7959
| 5′– | T | C | C | , | A | C | C | – | | 3′– | A | G | G | , | T | G | G | – | | | | – | T | T | A | , | G | T | C | , | T | G | T | , | T | G | C | , | C | A | C | , | A | G | A | , | A | G | C | , | T | G | G | – | | – | A | A | T | , | C | A | G | , | A | C | A | , | A | C | G | , | G | T | G | , | T | C | T | , | T | C | G | , | A | C | C | – | | | | – | A | C | C | , | T | A | G | –3′ | | – | T | G | G | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCCACC-3′ and 5′-CTAGGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 4bde_ca56
| 5′– | A | T | C | , | T | G | G | – | | 3′– | T | A | G | , | A | C | C | – | | | | – | T | G | C | , | A | T | C | , | C | C | A | , | T | C | G | , | A | T | G | , | T | A | C | , | G | A | G | , | A | T | C | – | | – | A | C | G | , | T | A | G | , | G | G | T | , | A | G | C | , | T | A | C | , | A | T | G | , | C | T | C | , | T | A | G | – | | | | – | T | C | A | , | G | A | C | –3′ | | – | A | G | T | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCTGG-3′ and 5′-GTCTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ff35_a801
| 5′– | C | C | T | , | C | G | A | – | | 3′– | G | G | A | , | G | C | T | – | | | | – | C | T | A | , | C | C | G | , | T | T | C | , | T | C | C | , | G | T | G | , | A | T | A | , | C | A | G | , | A | C | T | – | | – | G | A | T | , | G | G | C | , | A | A | G | , | A | G | G | , | C | A | C | , | T | A | T | , | G | T | C | , | T | G | A | – | | | | – | G | A | T | , | C | C | A | –3′ | | – | C | T | A | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTCGA-3′ and 5′-TGGATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC e72a_e860
| 5′– | T | G | G | , | A | G | C | – | | 3′– | A | C | C | , | T | C | G | – | | | | – | G | T | A | , | C | A | C | , | C | T | C | , | G | G | T | , | C | A | G | , | T | T | G | , | C | T | A | , | T | G | T | – | | – | C | A | T | , | G | T | G | , | G | A | G | , | C | C | A | , | G | T | C | , | A | A | C | , | G | A | T | , | A | C | A | – | | | | – | C | A | G | , | T | C | A | –3′ | | – | G | T | C | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGAGC-3′ and 5′-TGACTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC ab63_a881
| 5′– | C | A | A | , | G | T | A | – | | 3′– | G | T | T | , | C | A | T | – | | | | – | G | T | T | , | C | G | A | , | G | C | T | , | G | A | A | , | C | G | T | , | G | T | A | , | G | C | A | , | T | C | T | – | | – | C | A | A | , | G | C | T | , | C | G | A | , | C | T | T | , | G | C | A | , | C | A | T | , | C | G | T | , | A | G | A | – | | | | – | A | T | G | , | C | T | G | –3′ | | – | T | A | C | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAAGTA-3′ and 5′-CAGCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 95d8_e0f7
| 5′– | T | C | G | , | A | G | G | – | | 3′– | A | G | C | , | T | C | C | – | | | | – | A | G | G | , | T | G | C | , | T | T | T | , | G | A | C | , | G | G | T | , | C | A | G | , | A | T | G | , | C | A | C | – | | – | T | C | C | , | A | C | G | , | A | A | A | , | C | T | G | , | C | C | A | , | G | T | C | , | T | A | C | , | G | T | G | – | | | | – | T | A | C | , | T | T | G | –3′ | | – | A | T | G | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCGAGG-3′ and 5′-CAAGTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 481f_a627
| 5′– | A | C | G | , | C | T | G | – | | 3′– | T | G | C | , | G | A | C | – | | | | – | G | A | C | , | C | G | T | , | C | C | T | , | C | C | T | , | C | T | C | , | T | A | G | , | T | C | A | , | G | A | T | – | | – | C | T | G | , | G | C | A | , | G | G | A | , | G | G | A | , | G | A | G | , | A | T | C | , | A | G | T | , | C | T | A | – | | | | – | C | A | G | , | G | A | T | –3′ | | – | G | T | C | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACGCTG-3′ and 5′-ATCCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC eb4d_c858
| 5′– | C | A | G | , | T | G | A | – | | 3′– | G | T | C | , | A | C | T | – | | | | – | T | T | C | , | G | G | A | , | T | T | C | , | A | C | G | , | G | T | A | , | C | T | G | , | C | G | A | , | A | T | C | – | | – | A | A | G | , | C | C | T | , | A | A | G | , | T | G | C | , | C | A | T | , | G | A | C | , | G | C | T | , | T | A | G | – | | | | – | A | G | C | , | T | T | G | –3′ | | – | T | C | G | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGTGA-3′ and 5′-CAAGCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 6c30_358a
| 5′– | T | A | C | , | C | T | G | – | | 3′– | A | T | G | , | G | A | C | – | | | | – | A | T | G | , | T | T | C | , | T | C | T | , | C | G | A | , | C | A | T | , | C | T | C | , | T | T | A | , | G | T | C | – | | – | T | A | C | , | A | A | G | , | A | G | A | , | G | C | T | , | G | T | A | , | G | A | G | , | A | A | T | , | C | A | G | – | | | | – | C | T | A | , | C | C | T | –3′ | | – | G | A | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TACCTG-3′ and 5′-AGGTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 5370_d50c
| 5′– | A | A | C | , | T | A | C | – | | 3′– | T | T | G | , | A | T | G | – | | | | – | C | A | T | , | G | G | A | , | C | C | T | , | A | A | C | , | C | A | G | , | C | T | G | , | T | G | C | , | A | T | A | – | | – | G | T | A | , | C | C | T | , | G | G | A | , | T | T | G | , | G | T | C | , | G | A | C | , | A | C | G | , | T | A | T | – | | | | – | G | T | C | , | A | T | T | –3′ | | – | C | A | G | , | T | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACTAC-3′ and 5′-AATGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7eac_e59f
| 5′– | C | T | G | , | T | A | A | – | | 3′– | G | A | C | , | A | T | T | – | | | | – | A | T | C | , | A | C | G | , | T | A | C | , | C | T | T | , | A | C | T | , | T | G | A | , | T | G | A | , | T | C | C | – | | – | T | A | G | , | T | G | C | , | A | T | G | , | G | A | A | , | T | G | A | , | A | C | T | , | A | C | T | , | A | G | G | – | | | | – | T | T | G | , | G | A | C | –3′ | | – | A | A | C | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGTAA-3′ and 5′-GTCCAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 60b5_aef3
| 5′– | C | A | G | , | C | A | T | – | | 3′– | G | T | C | , | G | T | A | – | | | | – | T | A | G | , | C | C | T | , | C | T | T | , | C | C | G | , | A | T | A | , | G | A | C | , | T | C | A | , | A | C | G | – | | – | A | T | C | , | G | G | A | , | G | A | A | , | G | G | C | , | T | A | T | , | C | T | G | , | A | G | T | , | T | G | C | – | | | | – | A | C | T | , | T | A | G | –3′ | | – | T | G | A | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGCAT-3′ and 5′-CTAAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7ab5_b9ac
| 5′– | G | A | T | , | T | G | A | – | | 3′– | C | T | A | , | A | C | T | – | | | | – | T | T | C | , | C | A | G | , | G | G | A | , | C | A | T | , | A | G | G | , | A | A | A | , | G | A | T | , | C | G | C | – | | – | A | A | G | , | G | T | C | , | C | C | T | , | G | T | A | , | T | C | C | , | T | T | T | , | C | T | A | , | G | C | G | – | | | | – | A | G | T | , | G | C | C | –3′ | | – | T | C | A | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATTGA-3′ and 5′-GGCACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC ee6b_f23a
| 5′– | T | T | C | , | A | G | G | – | | 3′– | A | A | G | , | T | C | C | – | | | | – | G | C | T | , | C | C | A | , | G | A | A | , | T | C | T | , | T | G | G | , | T | T | C | , | G | T | G | , | C | A | T | – | | – | C | G | A | , | G | G | T | , | C | T | T | , | A | G | A | , | A | C | C | , | A | A | G | , | C | A | C | , | G | T | A | – | | | | – | A | G | T | , | A | G | C | –3′ | | – | T | C | A | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCAGG-3′ and 5′-GCTACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 480c_50a8
| 5′– | C | C | T | , | A | C | T | – | | 3′– | G | G | A | , | T | G | A | – | | | | – | T | C | A | , | G | G | A | , | A | G | T | , | G | T | A | , | A | C | T | , | C | C | T | , | T | C | A | , | C | C | G | – | | – | A | G | T | , | C | C | T | , | T | C | A | , | C | A | T | , | T | G | A | , | G | G | A | , | A | G | T | , | G | G | C | – | | | | – | G | A | A | , | A | G | T | –3′ | | – | C | T | T | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTACT-3′ and 5′-ACTTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC bc35_99b4
| 5′– | G | T | T | , | G | G | A | – | | 3′– | C | A | A | , | C | C | T | – | | | | – | C | A | C | , | T | G | T | , | C | A | G | , | C | A | G | , | G | C | A | , | C | G | A | , | T | C | T | , | G | T | A | – | | – | G | T | G | , | A | C | A | , | G | T | C | , | G | T | C | , | C | G | T | , | G | C | T | , | A | G | A | , | C | A | T | – | | | | – | A | G | T | , | A | C | C | –3′ | | – | T | C | A | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTGGA-3′ and 5′-GGTACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 704d_cb19
| 5′– | T | C | A | , | A | C | C | – | | 3′– | A | G | T | , | T | G | G | – | | | | – | A | G | C | , | C | T | C | , | T | A | G | , | T | T | T | , | C | C | T | , | G | T | T | , | G | T | A | , | C | T | G | – | | – | T | C | G | , | G | A | G | , | A | T | C | , | A | A | A | , | G | G | A | , | C | A | A | , | C | A | T | , | G | A | C | – | | | | – | T | A | G | , | A | C | C | –3′ | | – | A | T | C | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAACC-3′ and 5′-GGTCTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 4850_5aa6
| 5′– | C | G | G | , | A | G | T | – | | 3′– | G | C | C | , | T | C | A | – | | | | – | A | A | G | , | T | G | C | , | T | T | C | , | C | T | T | , | T | G | T | , | A | C | A | , | C | C | G | , | T | A | C | – | | – | T | T | C | , | A | C | G | , | A | A | G | , | G | A | A | , | A | C | A | , | T | G | T | , | G | G | C | , | A | T | G | – | | | | – | T | C | A | , | T | T | C | –3′ | | – | A | G | T | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGGAGT-3′ and 5′-GAATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4b16_7aa6
| 5′– | T | A | G | , | T | T | C | – | | 3′– | A | T | C | , | A | A | G | – | | | | – | C | G | C | , | A | T | T | , | C | C | C | , | T | A | G | , | A | G | A | , | C | A | A | , | C | T | G | , | G | C | A | – | | – | G | C | G | , | T | A | A | , | G | G | G | , | A | T | C | , | T | C | T | , | G | T | T | , | G | A | C | , | C | G | T | – | | | | – | A | T | G | , | C | T | G | –3′ | | – | T | A | C | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGTTC-3′ and 5′-CAGCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 181f_1a0a
| 5′– | A | G | T | , | G | T | C | – | | 3′– | T | C | A | , | C | A | G | – | | | | – | G | G | G | , | A | T | C | , | G | T | C | , | C | A | C | , | A | C | C | , | A | A | A | , | G | C | C | , | A | C | T | – | | – | C | C | C | , | T | A | G | , | C | A | G | , | G | T | G | , | T | G | G | , | T | T | T | , | C | G | G | , | T | G | A | – | | | | – | G | G | T | , | A | G | T | –3′ | | – | C | C | A | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTGTC-3′ and 5′-ACTACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0306_43db
| 5′– | T | G | A | , | C | G | G | – | | 3′– | A | C | T | , | G | C | C | – | | | | – | A | G | A | , | G | C | T | , | G | A | A | , | G | T | G | , | C | G | A | , | T | C | T | , | T | C | A | , | T | G | C | – | | – | T | C | T | , | C | G | A | , | C | T | T | , | C | A | C | , | G | C | T | , | A | G | A | , | A | G | T | , | A | C | G | – | | | | – | C | A | T | , | T | G | A | –3′ | | – | G | T | A | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGACGG-3′ and 5′-TCAATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3360_c3f2
| 5′– | C | T | A | , | C | A | A | – | | 3′– | G | A | T | , | G | T | T | – | | | | – | C | C | T | , | C | G | A | , | T | T | G | , | C | C | A | , | T | C | C | , | C | T | C | , | G | T | C | , | T | A | A | – | | – | G | G | A | , | G | C | T | , | A | A | C | , | G | G | T | , | A | G | G | , | G | A | G | , | C | A | G | , | A | T | T | – | | | | – | G | A | A | , | C | C | T | –3′ | | – | C | T | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTACAA-3′ and 5′-AGGTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC fb80_dedb
| 5′– | T | A | C | , | A | C | G | – | | 3′– | A | T | G | , | T | G | C | – | | | | – | T | T | C | , | G | A | G | , | C | C | T | , | C | A | G | , | G | A | A | , | A | C | T | , | A | C | G | , | T | A | G | – | | – | A | A | G | , | C | T | C | , | G | G | A | , | G | T | C | , | C | T | T | , | T | G | A | , | T | G | C | , | A | T | C | – | | | | – | T | G | G | , | A | A | G | –3′ | | – | A | C | C | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TACACG-3′ and 5′-CTTCCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC a19e_a822
| 5′– | C | G | T | , | A | C | T | – | | 3′– | G | C | A | , | T | G | A | – | | | | – | C | G | T | , | T | A | C | , | C | A | G | , | G | G | T | , | T | C | G | , | T | T | C | , | G | A | T | , | G | C | A | – | | – | G | C | A | , | A | T | G | , | G | T | C | , | C | C | A | , | A | G | C | , | A | A | G | , | C | T | A | , | C | G | T | – | | | | – | A | A | C | , | A | T | C | –3′ | | – | T | T | G | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTACT-3′ and 5′-GATGTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2719_3584
| 5′– | T | C | G | , | G | A | C | – | | 3′– | A | G | C | , | C | T | G | – | | | | – | G | A | C | , | G | G | T | , | C | A | A | , | T | C | G | , | T | T | C | , | C | T | C | , | A | G | C | , | T | T | A | – | | – | C | T | G | , | C | C | A | , | G | T | T | , | A | G | C | , | A | A | G | , | G | A | G | , | T | C | G | , | A | A | T | – | | | | – | A | T | G | , | A | C | C | –3′ | | – | T | A | C | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCGGAC-3′ and 5′-GGTCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC eba1_ad87
| 5′– | C | G | G | , | A | T | T | – | | 3′– | G | C | C | , | T | A | A | – | | | | – | A | T | A | , | C | G | C | , | T | A | C | , | T | G | A | , | G | C | A | , | T | G | G | , | A | G | T | , | C | C | C | – | | – | T | A | T | , | G | C | G | , | A | T | G | , | A | C | T | , | C | G | T | , | A | C | C | , | T | C | A | , | G | G | G | – | | | | – | G | C | T | , | A | G | T | –3′ | | – | C | G | A | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGGATT-3′ and 5′-ACTAGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ab3a_3f19
| 5′– | G | T | A | , | A | G | T | – | | 3′– | C | A | T | , | T | C | A | – | | | | – | C | A | T | , | G | T | C | , | C | T | A | , | C | T | T | , | T | C | T | , | C | T | C | , | T | C | G | , | C | A | A | – | | – | G | T | A | , | C | A | G | , | G | A | T | , | G | A | A | , | A | G | A | , | G | A | G | , | A | G | C | , | G | T | T | – | | | | – | G | C | C | , | A | C | T | –3′ | | – | C | G | G | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTAAGT-3′ and 5′-AGTGGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1e3b_4b7f
| 5′– | T | C | G | , | A | C | G | – | | 3′– | A | G | C | , | T | G | C | – | | | | – | A | A | T | , | G | A | C | , | A | T | C | , | A | A | G | , | T | G | G | , | C | T | C | , | A | C | T | , | A | G | G | – | | – | T | T | A | , | C | T | G | , | T | A | G | , | T | T | C | , | A | C | C | , | G | A | G | , | T | G | A | , | T | C | C | – | | | | – | T | T | C | , | T | A | G | –3′ | | – | A | A | G | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCGACG-3′ and 5′-CTAGAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 5c24_3601
| 5′– | C | T | A | , | G | A | A | – | | 3′– | G | A | T | , | C | T | T | – | | | | – | A | T | C | , | T | T | G | , | C | T | T | , | C | A | C | , | G | A | C | , | C | T | G | , | G | G | T | , | C | A | C | – | | – | T | A | G | , | A | A | C | , | G | A | A | , | G | T | G | , | C | T | G | , | G | A | C | , | C | C | A | , | G | T | G | – | | | | – | T | C | A | , | T | G | G | –3′ | | – | A | G | T | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTAGAA-3′ and 5′-CCATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 8f15_e5f9
| 5′– | A | A | C | , | A | T | C | – | | 3′– | T | T | G | , | T | A | G | – | | | | – | T | G | C | , | G | A | A | , | T | G | T | , | A | T | G | , | G | A | C | , | T | T | C | , | G | T | G | , | A | G | C | – | | – | A | C | G | , | C | T | T | , | A | C | A | , | T | A | C | , | C | T | G | , | A | A | G | , | C | A | C | , | T | C | G | – | | | | – | A | T | T | , | C | T | G | –3′ | | – | T | A | A | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACATC-3′ and 5′-CAGAAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4411_6bd8
| 5′– | C | A | T | , | T | C | A | – | | 3′– | G | T | A | , | A | G | T | – | | | | – | C | T | A | , | G | T | T | , | A | G | A | , | A | A | G | , | G | G | A | , | A | C | G | , | A | C | T | , | C | G | T | – | | – | G | A | T | , | C | A | A | , | T | C | T | , | T | T | C | , | C | C | T | , | T | G | C | , | T | G | A | , | G | C | A | – | | | | – | T | A | C | , | T | A | G | –3′ | | – | A | T | G | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATTCA-3′ and 5′-CTAGTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 53ac_e46f
| 5′– | C | T | G | , | G | A | T | – | | 3′– | G | A | C | , | C | T | A | – | | | | – | G | T | T | , | A | C | T | , | C | A | T | , | A | G | G | , | C | A | C | , | G | C | T | , | T | G | T | , | C | G | A | – | | – | C | A | A | , | T | G | A | , | G | T | A | , | T | C | C | , | G | T | G | , | C | G | A | , | A | C | A | , | G | C | T | – | | | | – | G | A | C | , | T | T | A | –3′ | | – | C | T | G | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGGAT-3′ and 5′-TAAGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c044_ac4c
| 5′– | A | C | C | , | T | G | C | – | | 3′– | T | G | G | , | A | C | G | – | | | | – | T | T | C | , | C | G | A | , | T | C | A | , | C | T | T | , | A | C | A | , | C | G | A | , | A | T | C | , | T | G | C | – | | – | A | A | G | , | G | C | T | , | A | G | T | , | G | A | A | , | T | G | T | , | G | C | T | , | T | A | G | , | A | C | G | – | | | | – | C | C | A | , | C | G | T | –3′ | | – | G | G | T | , | G | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACCTGC-3′ and 5′-ACGTGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC cef2_bfe0
| 5′– | T | A | G | , | A | G | G | – | | 3′– | A | T | C | , | T | C | C | – | | | | – | A | G | T | , | C | T | T | , | C | C | C | , | A | T | T | , | C | C | T | , | A | G | C | , | A | G | T | , | C | A | G | – | | – | T | C | A | , | G | A | A | , | G | G | G | , | T | A | A | , | G | G | A | , | T | C | G | , | T | C | A | , | G | T | C | – | | | | – | C | G | G | , | T | A | A | –3′ | | – | G | C | C | , | A | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGAGG-3′ and 5′-TTACCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 89e7_5c37
| 5′– | C | A | A | , | C | C | T | – | | 3′– | G | T | T | , | G | G | A | – | | | | – | T | A | C | , | T | A | G | , | T | C | T | , | G | T | T | , | C | T | G | , | C | T | C | , | T | G | C | , | T | A | G | – | | – | A | T | G | , | A | T | C | , | A | G | A | , | C | A | A | , | G | A | C | , | G | A | G | , | A | C | G | , | A | T | C | – | | | | – | A | T | G | , | A | C | C | –3′ | | – | T | A | C | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAACCT-3′ and 5′-GGTCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5e5b_90b5
| 5′– | G | C | A | , | G | T | A | – | | 3′– | C | G | T | , | C | A | T | – | | | | – | C | G | C | , | A | G | T | , | C | C | T | , | C | C | T | , | G | C | C | , | T | G | G | , | C | T | G | , | G | A | T | – | | – | G | C | G | , | T | C | A | , | G | G | A | , | G | G | A | , | C | G | G | , | A | C | C | , | G | A | C | , | C | T | A | – | | | | – | A | A | C | , | T | C | G | –3′ | | – | T | T | G | , | A | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCAGTA-3′ and 5′-CGAGTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0bc0_ef7e
| 5′– | G | T | C | , | A | G | T | – | | 3′– | C | A | G | , | T | C | A | – | | | | – | A | A | T | , | G | C | C | , | A | T | G | , | C | A | A | , | G | T | G | , | T | T | C | , | A | C | C | , | T | A | G | – | | – | T | T | A | , | C | G | G | , | T | A | C | , | G | T | T | , | C | A | C | , | A | A | G | , | T | G | G | , | A | T | C | – | | | | – | C | G | A | , | A | C | T | –3′ | | – | G | C | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCAGT-3′ and 5′-AGTTCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 672e_1dda
| 5′– | A | T | C | , | A | C | C | – | | 3′– | T | A | G | , | T | G | G | – | | | | – | T | C | G | , | A | T | T | , | G | G | A | , | C | T | G | , | C | A | A | , | A | C | A | , | A | C | G | , | G | T | G | – | | – | A | G | C | , | T | A | A | , | C | C | T | , | G | A | C | , | G | T | T | , | T | G | T | , | T | G | C | , | C | A | C | – | | | | – | G | G | C | , | A | A | T | –3′ | | – | C | C | G | , | T | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCACC-3′ and 5′-ATTGCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 7da5_6644
| 5′– | T | A | A | , | C | T | G | – | | 3′– | A | T | T | , | G | A | C | – | | | | – | T | A | C | , | C | G | T | , | C | A | T | , | T | G | T | , | T | A | C | , | G | C | A | , | T | A | C | , | T | G | C | – | | – | A | T | G | , | G | C | A | , | G | T | A | , | A | C | A | , | A | T | G | , | C | G | T | , | A | T | G | , | A | C | G | – | | | | – | T | G | C | , | G | A | C | –3′ | | – | A | C | G | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAACTG-3′ and 5′-GTCGCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 9bb6_ae09
| 5′– | T | C | A | , | A | T | C | – | | 3′– | A | G | T | , | T | A | G | – | | | | – | G | T | C | , | T | G | A | , | C | T | A | , | A | G | T | , | G | T | T | , | A | G | G | , | T | G | G | , | C | A | T | – | | – | C | A | G | , | A | C | T | , | G | A | T | , | T | C | A | , | C | A | A | , | T | C | C | , | A | C | C | , | G | T | A | – | | | | – | A | A | C | , | T | A | C | –3′ | | – | T | T | G | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAATC-3′ and 5′-GTAGTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 547a_8b1f
| 5′– | G | T | C | , | A | T | T | – | | 3′– | C | A | G | , | T | A | A | – | | | | – | G | A | A | , | T | C | A | , | C | T | T | , | T | C | G | , | A | G | T | , | G | G | G | , | T | G | T | , | C | A | A | – | | – | C | T | T | , | A | G | T | , | G | A | A | , | A | G | C | , | T | C | A | , | C | C | C | , | A | C | A | , | G | T | T | – | | | | – | C | C | A | , | C | C | T | –3′ | | – | G | G | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCATT-3′ and 5′-AGGTGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4e2a_a291
| 5′– | A | T | C | , | C | T | G | – | | 3′– | T | A | G | , | G | A | C | – | | | | – | T | A | G | , | T | G | C | , | T | G | A | , | G | T | T | , | C | A | T | , | G | T | G | , | G | C | T | , | C | A | G | – | | – | A | T | C | , | A | C | G | , | A | C | T | , | C | A | A | , | G | T | A | , | C | A | C | , | C | G | A | , | G | T | C | – | | | | – | C | A | A | , | T | G | A | –3′ | | – | G | T | T | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCCTG-3′ and 5′-TCATTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC e234_cbba
| 5′– | T | G | A | , | A | A | C | – | | 3′– | A | C | T | , | T | T | G | – | | | | – | A | T | A | , | G | T | C | , | T | G | G | , | T | T | C | , | C | A | G | , | G | T | C | , | T | A | G | , | G | A | C | – | | – | T | A | T | , | C | A | G | , | A | C | C | , | A | A | G | , | G | T | C | , | C | A | G | , | A | T | C | , | C | T | G | – | | | | – | G | C | C | , | T | T | A | –3′ | | – | C | G | G | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAAAC-3′ and 5′-TAAGGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 7a90_836c
| 5′– | A | T | G | , | A | A | C | – | | 3′– | T | A | C | , | T | T | G | – | | | | – | G | C | A | , | G | C | T | , | C | C | G | , | A | A | A | , | G | A | A | , | G | T | T | , | A | G | A | , | C | T | T | – | | – | C | G | T | , | C | G | A | , | G | G | C | , | T | T | T | , | C | T | T | , | C | A | A | , | T | C | T | , | G | A | A | – | | | | – | G | G | T | , | G | A | A | –3′ | | – | C | C | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGAAC-3′ and 5′-TTCACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 1707_1845
| 5′– | A | C | T | , | A | T | G | – | | 3′– | T | G | A | , | T | A | C | – | | | | – | C | G | C | , | T | C | A | , | A | C | G | , | A | T | G | , | A | G | C | , | T | C | C | , | G | T | A | , | A | C | A | – | | – | G | C | G | , | A | G | T | , | T | G | C | , | T | A | C | , | T | C | G | , | A | G | G | , | C | A | T | , | T | G | T | – | | | | – | G | A | T | , | G | G | A | –3′ | | – | C | T | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTATG-3′ and 5′-TCCATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 876c_43f2
| 5′– | A | T | T | , | C | T | G | – | | 3′– | T | A | A | , | G | A | C | – | | | | – | C | A | T | , | T | C | G | , | T | A | A | , | C | A | A | , | C | T | A | , | C | C | A | , | C | C | T | , | G | A | A | – | | – | G | T | A | , | A | G | C | , | A | T | T | , | G | T | T | , | G | A | T | , | G | G | T | , | G | G | A | , | C | T | T | – | | | | – | G | T | A | , | C | G | T | –3′ | | – | C | A | T | , | G | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATTCTG-3′ and 5′-ACGTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 31c8_2922
| 5′– | C | G | A | , | C | A | T | – | | 3′– | G | C | T | , | G | T | A | – | | | | – | C | T | G | , | T | C | A | , | G | T | T | , | G | A | A | , | G | A | G | , | T | T | A | , | C | T | C | , | G | G | A | – | | – | G | A | C | , | A | G | T | , | C | A | A | , | C | T | T | , | C | T | C | , | A | A | T | , | G | A | G | , | C | C | T | – | | | | – | G | A | A | , | A | G | T | –3′ | | – | C | T | T | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGACAT-3′ and 5′-ACTTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC a944_c9ed
| 5′– | G | C | A | , | T | G | A | – | | 3′– | C | G | T | , | A | C | T | – | | | | – | T | A | A | , | G | C | T | , | C | C | T | , | T | A | C | , | T | G | A | , | C | T | T | , | G | T | T | , | A | G | C | – | | – | A | T | T | , | C | G | A | , | G | G | A | , | A | T | G | , | A | C | T | , | G | A | A | , | C | A | A | , | T | C | G | – | | | | – | T | G | C | , | T | A | C | –3′ | | – | A | C | G | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCATGA-3′ and 5′-GTAGCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC ccf4_0134
| 5′– | G | C | T | , | A | C | T | – | | 3′– | C | G | A | , | T | G | A | – | | | | – | T | C | G | , | T | C | A | , | T | C | G | , | A | G | C | , | A | C | C | , | C | A | T | , | G | C | A | , | G | T | C | – | | – | A | G | C | , | A | G | T | , | A | G | C | , | T | C | G | , | T | G | G | , | G | T | A | , | C | G | T | , | C | A | G | – | | | | – | T | G | A | , | T | T | C | –3′ | | – | A | C | T | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTACT-3′ and 5′-GAATCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 53c6_597c
| 5′– | G | G | A | , | G | C | T | – | | 3′– | C | C | T | , | C | G | A | – | | | | – | G | A | G | , | C | G | T | , | T | T | G | , | G | T | C | , | G | A | G | , | G | T | A | , | G | A | T | , | A | C | A | – | | – | C | T | C | , | G | C | A | , | A | A | C | , | C | A | G | , | C | T | C | , | C | A | T | , | C | T | A | , | T | G | T | – | | | | – | C | C | A | , | C | T | T | –3′ | | – | G | G | T | , | G | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGAGCT-3′ and 5′-AAGTGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 097c_d022
| 5′– | G | A | T | , | T | G | A | – | | 3′– | C | T | A | , | A | C | T | – | | | | – | A | T | G | , | C | G | A | , | G | A | C | , | A | G | T | , | G | G | T | , | T | A | C | , | A | T | G | , | G | T | C | – | | – | T | A | C | , | G | C | T | , | C | T | G | , | T | C | A | , | C | C | A | , | A | T | G | , | T | A | C | , | C | A | G | – | | | | – | A | T | C | , | T | G | C | –3′ | | – | T | A | G | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATTGA-3′ and 5′-GCAGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 8406_e022
| 5′– | A | T | T | , | C | T | G | – | | 3′– | T | A | A | , | G | A | C | – | | | | – | G | T | A | , | T | C | T | , | C | G | C | , | A | C | G | , | C | T | T | , | G | C | A | , | G | G | A | , | C | G | T | – | | – | C | A | T | , | A | G | A | , | G | C | G | , | T | G | C | , | G | A | A | , | C | G | T | , | C | C | T | , | G | C | A | – | | | | – | C | T | A | , | A | G | T | –3′ | | – | G | A | T | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATTCTG-3′ and 5′-ACTTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC d461_5a71
| 5′– | A | G | C | , | T | C | C | – | | 3′– | T | C | G | , | A | G | G | – | | | | – | A | G | T | , | C | G | C | , | A | C | T | , | T | C | G | , | T | A | C | , | G | C | T | , | T | A | C | , | T | A | G | – | | – | T | C | A | , | G | C | G | , | T | G | A | , | A | G | C | , | A | T | G | , | C | G | A | , | A | T | G | , | A | T | C | – | | | | – | T | G | A | , | G | C | C | –3′ | | – | A | C | T | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGCTCC-3′ and 5′-GGCTCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5e80_0c1a
| 5′– | A | T | G | , | C | T | G | – | | 3′– | T | A | C | , | G | A | C | – | | | | – | A | G | A | , | T | A | C | , | T | T | G | , | G | A | A | , | T | C | T | , | A | G | A | , | G | A | C | , | T | G | G | – | | – | T | C | T | , | A | T | G | , | A | A | C | , | C | T | T | , | A | G | A | , | T | C | T | , | C | T | G | , | A | C | C | – | | | | – | C | A | T | , | G | G | T | –3′ | | – | G | T | A | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGCTG-3′ and 5′-ACCATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC e0a2_d98e
| 5′– | C | G | A | , | C | T | T | – | | 3′– | G | C | T | , | G | A | A | – | | | | – | G | G | A | , | A | C | T | , | C | G | T | , | G | T | A | , | T | C | C | , | C | A | C | , | C | A | G | , | T | T | A | – | | – | C | C | T | , | T | G | A | , | G | C | A | , | C | A | T | , | A | G | G | , | G | T | G | , | G | T | C | , | A | A | T | – | | | | – | T | G | A | , | G | T | C | –3′ | | – | A | C | T | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGACTT-3′ and 5′-GACTCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 8af6_d708
| 5′– | G | G | A | , | A | C | T | – | | 3′– | C | C | T | , | T | G | A | – | | | | – | A | A | G | , | A | T | C | , | C | C | T | , | C | G | A | , | G | A | G | , | T | C | G | , | G | T | C | , | T | A | C | – | | – | T | T | C | , | T | A | G | , | G | G | A | , | G | C | T | , | C | T | C | , | A | G | C | , | C | A | G | , | A | T | G | – | | | | – | G | T | C | , | T | G | A | –3′ | | – | C | A | G | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGAACT-3′ and 5′-TCAGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 66ec_e408
| 5′– | G | T | A | , | G | A | T | – | | 3′– | C | A | T | , | C | T | A | – | | | | – | A | A | A | , | C | G | T | , | T | A | G | , | T | G | G | , | G | A | A | , | C | C | C | , | A | T | T | , | C | G | G | – | | – | T | T | T | , | G | C | A | , | A | T | C | , | A | C | C | , | C | T | T | , | G | G | G | , | T | A | A | , | G | C | C | – | | | | – | C | T | G | , | A | A | T | –3′ | | – | G | A | C | , | T | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTAGAT-3′ and 5′-ATTCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 52c9_db8b
| 5′– | G | T | T | , | G | A | T | – | | 3′– | C | A | A | , | C | T | A | – | | | | – | C | G | T | , | G | A | A | , | C | C | C | , | A | G | A | , | C | A | A | , | A | G | T | , | T | A | G | , | A | C | T | – | | – | G | C | A | , | C | T | T | , | G | G | G | , | T | C | T | , | G | T | T | , | T | C | A | , | A | T | C | , | T | G | A | – | | | | – | T | A | G | , | T | G | C | –3′ | | – | A | T | C | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTGAT-3′ and 5′-GCACTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC d8bb_bfd7
| 5′– | A | A | C | , | C | T | G | – | | 3′– | T | T | G | , | G | A | C | – | | | | – | A | A | C | , | C | T | G | , | A | G | G | , | T | G | A | , | G | T | G | , | A | A | G | , | G | A | G | , | A | T | C | – | | – | T | T | G | , | G | A | C | , | T | C | C | , | A | C | T | , | C | A | C | , | T | T | C | , | C | T | C | , | T | A | G | – | | | | – | T | G | G | , | A | G | C | –3′ | | – | A | C | C | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACCTG-3′ and 5′-GCTCCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC cc55_83ec
| 5′– | T | A | C | , | G | A | C | – | | 3′– | A | T | G | , | C | T | G | – | | | | – | A | G | C | , | G | T | T | , | A | G | A | , | G | A | C | , | T | A | C | , | G | A | C | , | A | A | T | , | C | G | G | – | | – | T | C | G | , | C | A | A | , | T | C | T | , | C | T | G | , | A | T | G | , | C | T | G | , | T | T | A | , | G | C | C | – | | | | – | C | C | A | , | C | A | T | –3′ | | – | G | G | T | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TACGAC-3′ and 5′-ATGTGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1053_8803
| 5′– | G | C | C | , | T | T | A | – | | 3′– | C | G | G | , | A | A | T | – | | | | – | A | A | T | , | G | C | T | , | C | C | A | , | T | G | G | , | A | A | G | , | C | A | G | , | A | T | C | , | A | C | G | – | | – | T | T | A | , | C | G | A | , | G | G | T | , | A | C | C | , | T | T | C | , | G | T | C | , | T | A | G | , | T | G | C | – | | | | – | A | C | T | , | T | T | C | –3′ | | – | T | G | A | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCCTTA-3′ and 5′-GAAAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 8838_a141
| 5′– | A | G | C | , | T | G | C | – | | 3′– | T | C | G | , | A | C | G | – | | | | – | G | C | G | , | T | G | A | , | A | C | T | , | C | G | T | , | T | G | A | , | G | T | G | , | T | C | T | , | G | A | T | – | | – | C | G | C | , | A | C | T | , | T | G | A | , | G | C | A | , | A | C | T | , | C | A | C | , | A | G | A | , | C | T | A | – | | | | – | C | G | G | , | A | G | T | –3′ | | – | G | C | C | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGCTGC-3′ and 5′-ACTCCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC f7a4_dc6b
| 5′– | T | T | C | , | G | A | C | – | | 3′– | A | A | G | , | C | T | G | – | | | | – | T | A | G | , | A | C | G | , | T | G | A | , | T | C | C | , | C | A | C | , | A | A | G | , | T | G | C | , | A | G | C | – | | – | A | T | C | , | T | G | C | , | A | C | T | , | A | G | G | , | G | T | G | , | T | T | C | , | A | C | G | , | T | C | G | – | | | | – | C | T | G | , | G | G | A | –3′ | | – | G | A | C | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCGAC-3′ and 5′-TCCCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b96f_fc1a
| 5′– | T | C | A | , | A | G | G | – | | 3′– | A | G | T | , | T | C | C | – | | | | – | T | C | C | , | T | G | A | , | A | G | A | , | C | C | T | , | C | G | T | , | C | C | T | , | A | C | T | , | C | C | G | – | | – | A | G | G | , | A | C | T | , | T | C | T | , | G | G | A | , | G | C | A | , | G | G | A | , | T | G | A | , | G | G | C | – | | | | – | A | T | C | , | A | A | C | –3′ | | – | T | A | G | , | T | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAAGG-3′ and 5′-GTTGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3e50_70c6
| 5′– | T | C | A | , | T | T | C | – | | 3′– | A | G | T | , | A | A | G | – | | | | – | T | C | C | , | G | T | A | , | T | C | A | , | C | G | T | , | T | C | G | , | A | C | C | , | A | G | G | , | G | T | C | – | | – | A | G | G | , | C | A | T | , | A | G | T | , | G | C | A | , | A | G | C | , | T | G | G | , | T | C | C | , | C | A | G | – | | | | – | C | T | A | , | G | G | A | –3′ | | – | G | A | T | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCATTC-3′ and 5′-TCCTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 6bfa_21b9
| 5′– | T | G | G | , | C | A | G | – | | 3′– | A | C | C | , | G | T | C | – | | | | – | A | C | T | , | C | G | G | , | T | T | A | , | C | A | T | , | A | G | G | , | A | T | C | , | C | G | T | , | G | A | C | – | | – | T | G | A | , | G | C | C | , | A | A | T | , | G | T | A | , | T | C | C | , | T | A | G | , | G | C | A | , | C | T | G | – | | | | – | A | T | C | , | A | A | G | –3′ | | – | T | A | G | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGCAG-3′ and 5′-CTTGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC af9e_5562
| 5′– | A | T | C | , | T | G | C | – | | 3′– | T | A | G | , | A | C | G | – | | | | – | C | T | A | , | G | G | A | , | G | A | A | , | G | C | T | , | G | G | T | , | G | C | G | , | A | G | C | , | G | T | A | – | | – | G | A | T | , | C | C | T | , | C | T | T | , | C | G | A | , | C | C | A | , | C | G | C | , | T | C | G | , | C | A | T | – | | | | – | T | A | A | , | C | G | G | –3′ | | – | A | T | T | , | G | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCTGC-3′ and 5′-CCGTTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5844_5ec7
| 5′– | T | G | G | , | T | A | C | – | | 3′– | A | C | C | , | A | T | G | – | | | | – | A | G | A | , | A | T | C | , | A | A | A | , | C | T | T | , | C | C | T | , | T | T | C | , | T | T | C | , | A | G | G | – | | – | T | C | T | , | T | A | G | , | T | T | T | , | G | A | A | , | G | G | A | , | A | A | G | , | A | A | G | , | T | C | C | – | | | | – | A | T | G | , | A | T | C | –3′ | | – | T | A | C | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGTAC-3′ and 5′-GATCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ba88_ec52
| 5′– | A | G | G | , | T | G | C | – | | 3′– | T | C | C | , | A | C | G | – | | | | – | G | C | A | , | C | T | G | , | G | A | A | , | G | C | A | , | A | T | G | , | T | T | A | , | G | T | A | , | C | G | T | – | | – | C | G | T | , | G | A | C | , | C | T | T | , | C | G | T | , | T | A | C | , | A | A | T | , | C | A | T | , | G | C | A | – | | | | – | C | T | T | , | G | T | A | –3′ | | – | G | A | A | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTGC-3′ and 5′-TACAAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC efd7_9e5b
| 5′– | A | A | C | , | T | A | C | – | | 3′– | T | T | G | , | A | T | G | – | | | | – | A | A | C | , | C | T | G | , | C | A | G | , | G | T | G | , | C | A | C | , | A | T | G | , | T | A | A | , | G | G | C | – | | – | T | T | G | , | G | A | C | , | G | T | C | , | C | A | C | , | G | T | G | , | T | A | C | , | A | T | T | , | C | C | G | – | | | | – | T | G | A | , | G | T | C | –3′ | | – | A | C | T | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACTAC-3′ and 5′-GACTCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2b9f_c75e
| 5′– | C | C | G | , | T | C | A | – | | 3′– | G | G | C | , | A | G | T | – | | | | – | T | G | C | , | A | A | C | , | G | T | G | , | T | T | C | , | G | A | A | , | C | C | T | , | C | A | A | , | C | T | G | – | | – | A | C | G | , | T | T | G | , | C | A | C | , | A | A | G | , | C | T | T | , | G | G | A | , | G | T | T | , | G | A | C | – | | | | – | T | C | A | , | T | A | G | –3′ | | – | A | G | T | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCGTCA-3′ and 5′-CTATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 7a79_a537
| 5′– | A | C | T | , | C | T | G | – | | 3′– | T | G | A | , | G | A | C | – | | | | – | C | A | C | , | C | G | T | , | A | C | G | , | A | C | T | , | G | C | A | , | G | G | T | , | G | A | C | , | A | G | T | – | | – | G | T | G | , | G | C | A | , | T | G | C | , | T | G | A | , | C | G | T | , | C | C | A | , | C | T | G | , | T | C | A | – | | | | – | A | T | G | , | C | T | G | –3′ | | – | T | A | C | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTCTG-3′ and 5′-CAGCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b48d_b006
| 5′– | T | G | G | , | A | C | G | – | | 3′– | A | C | C | , | T | G | C | – | | | | – | T | A | C | , | G | G | A | , | T | G | C | , | A | G | C | , | C | A | A | , | C | T | A | , | T | G | G | , | A | C | C | – | | – | A | T | G | , | C | C | T | , | A | C | G | , | T | C | G | , | G | T | T | , | G | A | T | , | A | C | C | , | T | G | G | – | | | | – | G | C | C | , | A | G | T | –3′ | | – | C | G | G | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGACG-3′ and 5′-ACTGGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6b3d_a91b
| 5′– | C | G | G | , | A | C | T | – | | 3′– | G | C | C | , | T | G | A | – | | | | – | C | C | A | , | G | T | C | , | G | A | C | , | C | C | A | , | C | G | G | , | A | A | G | , | A | C | G | , | C | T | A | – | | – | G | G | T | , | C | A | G | , | C | T | G | , | G | G | T | , | G | C | C | , | T | T | C | , | T | G | C | , | G | A | T | – | | | | – | A | G | T | , | T | A | G | –3′ | | – | T | C | A | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGGACT-3′ and 5′-CTAACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC d98b_6570
| 5′– | G | G | C | , | A | A | T | – | | 3′– | C | C | G | , | T | T | A | – | | | | – | T | C | T | , | T | A | G | , | A | C | A | , | A | C | G | , | C | A | T | , | C | A | G | , | G | T | A | , | T | C | G | – | | – | A | G | A | , | A | T | C | , | T | G | T | , | T | G | C | , | G | T | A | , | G | T | C | , | C | A | T | , | A | G | C | – | | | | – | C | A | A | , | C | C | T | –3′ | | – | G | T | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGCAAT-3′ and 5′-AGGTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 09da_9cdc
| 5′– | G | A | T | , | A | G | T | – | | 3′– | C | T | A | , | T | C | A | – | | | | – | A | G | T | , | G | C | G | , | T | C | C | , | T | G | C | , | G | T | G | , | C | A | C | , | C | G | T | , | G | A | G | – | | – | T | C | A | , | C | G | C | , | A | G | G | , | A | C | G | , | C | A | C | , | G | T | G | , | G | C | A | , | C | T | C | – | | | | – | G | T | T | , | G | A | A | –3′ | | – | C | A | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATAGT-3′ and 5′-TTCAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC d656_f8ac
| 5′– | T | A | G | , | G | A | C | – | | 3′– | A | T | C | , | C | T | G | – | | | | – | T | C | T | , | G | A | A | , | A | G | A | , | G | A | G | , | A | A | G | , | A | C | C | , | T | A | A | , | C | T | G | – | | – | A | G | A | , | C | T | T | , | T | C | T | , | C | T | C | , | T | T | C | , | T | G | G | , | A | T | T | , | G | A | C | – | | | | – | C | A | G | , | C | G | T | –3′ | | – | G | T | C | , | G | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGGAC-3′ and 5′-ACGCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 79dd_39ed
| 5′– | A | C | T | , | A | A | C | – | | 3′– | T | G | A | , | T | T | G | – | | | | – | A | T | C | , | A | G | T | , | G | G | T | , | C | C | A | , | C | A | T | , | T | C | G | , | A | G | A | , | A | T | C | – | | – | T | A | G | , | T | C | A | , | C | C | A | , | G | G | T | , | G | T | A | , | A | G | C | , | T | C | T | , | T | A | G | – | | | | – | G | C | A | , | T | G | A | –3′ | | – | C | G | T | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTAAC-3′ and 5′-TCATGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC cca2_fe69
| 5′– | A | A | C | , | C | T | G | – | | 3′– | T | T | G | , | G | A | C | – | | | | – | T | C | G | , | C | A | G | , | G | A | A | , | A | G | T | , | A | C | G | , | A | G | T | , | C | T | C | , | A | C | G | – | | – | A | G | C | , | G | T | C | , | C | T | T | , | T | C | A | , | T | G | C | , | T | C | A | , | G | A | G | , | T | G | C | – | | | | – | C | A | G | , | G | T | A | –3′ | | – | G | T | C | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACCTG-3′ and 5′-TACCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c11a_a528
| 5′– | C | T | A | , | G | A | A | – | | 3′– | G | A | T | , | C | T | T | – | | | | – | A | G | C | , | C | T | G | , | T | G | G | , | C | T | C | , | T | C | A | , | A | A | C | , | G | T | T | , | C | A | G | – | | – | T | C | G | , | G | A | C | , | A | C | C | , | G | A | G | , | A | G | T | , | T | T | G | , | C | A | A | , | G | T | C | – | | | | – | G | C | T | , | G | A | T | –3′ | | – | C | G | A | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTAGAA-3′ and 5′-ATCAGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 13a9_f5fe
| 5′– | T | A | G | , | T | C | G | – | | 3′– | A | T | C | , | A | G | C | – | | | | – | A | A | G | , | A | C | T | , | C | T | G | , | C | T | A | , | G | T | G | , | C | A | A | , | T | G | A | , | C | C | C | – | | – | T | T | C | , | T | G | A | , | G | A | C | , | G | A | T | , | C | A | C | , | G | T | T | , | A | C | T | , | G | G | G | – | | | | – | G | A | C | , | C | G | T | –3′ | | – | C | T | G | , | G | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGTCG-3′ and 5′-ACGGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC dfdb_1e6d
| 5′– | A | G | G | , | T | C | G | – | | 3′– | T | C | C | , | A | G | C | – | | | | – | C | T | C | , | G | C | A | , | C | A | A | , | C | T | T | , | A | C | T | , | G | A | C | , | T | C | G | , | G | T | A | – | | – | G | A | G | , | C | G | T | , | G | T | T | , | G | A | A | , | T | G | A | , | C | T | G | , | A | G | C | , | C | A | T | – | | | | – | A | T | G | , | A | G | C | –3′ | | – | T | A | C | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTCG-3′ and 5′-GCTCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC a19b_a056
| 5′– | A | A | G | , | T | C | C | – | | 3′– | T | T | C | , | A | G | G | – | | | | – | T | A | C | , | G | A | G | , | C | G | T | , | C | T | A | , | G | T | C | , | A | T | G | , | T | G | A | , | G | A | C | – | | – | A | T | G | , | C | T | C | , | G | C | A | , | G | A | T | , | C | A | G | , | T | A | C | , | A | C | T | , | C | T | G | – | | | | – | A | T | T | , | G | G | C | –3′ | | – | T | A | A | , | C | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AAGTCC-3′ and 5′-GCCAAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC a641_daad
| 5′– | T | G | A | , | A | C | C | – | | 3′– | A | C | T | , | T | G | G | – | | | | – | G | T | A | , | C | A | G | , | A | C | A | , | G | G | A | , | G | G | G | , | T | T | C | , | T | G | T | , | C | G | A | – | | – | C | A | T | , | G | T | C | , | T | G | T | , | C | C | T | , | C | C | C | , | A | A | G | , | A | C | A | , | G | C | T | – | | | | – | T | G | C | , | A | G | G | –3′ | | – | A | C | G | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAACC-3′ and 5′-CCTGCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 1331_26ef
| 5′– | C | G | A | , | G | T | A | – | | 3′– | G | C | T | , | C | A | T | – | | | | – | T | G | A | , | C | A | A | , | C | A | C | , | G | C | T | , | C | T | A | , | T | G | C | , | T | G | C | , | T | A | G | – | | – | A | C | T | , | G | T | T | , | G | T | G | , | C | G | A | , | G | A | T | , | A | C | G | , | A | C | G | , | A | T | C | – | | | | – | A | A | T | , | C | A | G | –3′ | | – | T | T | A | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGAGTA-3′ and 5′-CTGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 411e_3e4b
| 5′– | T | C | A | , | T | A | C | – | | 3′– | A | G | T | , | A | T | G | – | | | | – | C | A | G | , | T | A | C | , | T | G | G | , | C | T | T | , | C | C | T | , | A | G | G | , | T | G | C | , | A | A | T | – | | – | G | T | C | , | A | T | G | , | A | C | C | , | G | A | A | , | G | G | A | , | T | C | C | , | A | C | G | , | T | T | A | – | | | | – | T | A | A | , | C | T | G | –3′ | | – | A | T | T | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCATAC-3′ and 5′-CAGTTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b454_01cf
| 5′– | C | C | A | , | A | G | T | – | | 3′– | G | G | T | , | T | C | A | – | | | | – | C | G | T | , | G | G | A | , | G | G | A | , | T | T | G | , | C | G | T | , | G | T | G | , | T | C | A | , | G | T | A | – | | – | G | C | A | , | C | C | T | , | C | C | T | , | A | A | C | , | G | C | A | , | C | A | C | , | A | G | T | , | C | A | T | – | | | | – | C | C | G | , | T | T | A | –3′ | | – | G | G | C | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCAAGT-3′ and 5′-TAACGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC e0d8_207d
| 5′– | G | T | C | , | G | C | A | – | | 3′– | C | A | G | , | C | G | T | – | | | | – | T | C | C | , | C | A | G | , | C | C | T | , | G | T | T | , | G | T | C | , | A | A | C | , | G | T | G | , | A | G | C | – | | – | A | G | G | , | G | T | C | , | G | G | A | , | C | A | A | , | C | A | G | , | T | T | G | , | C | A | C | , | T | C | G | – | | | | – | G | G | C | , | A | T | T | –3′ | | – | C | C | G | , | T | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCGCA-3′ and 5′-AATGCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9c31_8069
| 5′– | T | C | A | , | C | A | G | – | | 3′– | A | G | T | , | G | T | C | – | | | | – | G | C | A | , | C | G | T | , | T | A | G | , | G | A | A | , | C | A | A | , | T | G | A | , | T | C | G | , | T | T | A | – | | – | C | G | T | , | G | C | A | , | A | T | C | , | C | T | T | , | G | T | T | , | A | C | T | , | A | G | C | , | A | A | T | – | | | | – | G | T | A | , | G | C | T | –3′ | | – | C | A | T | , | C | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCACAG-3′ and 5′-AGCTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 85f5_d911
| 5′– | C | C | G | , | A | T | T | – | | 3′– | G | G | C | , | T | A | A | – | | | | – | C | G | T | , | A | T | C | , | G | C | A | , | G | T | T | , | T | G | A | , | C | T | A | , | A | C | G | , | G | T | A | – | | – | G | C | A | , | T | A | G | , | C | G | T | , | C | A | A | , | A | C | T | , | G | A | T | , | T | G | C | , | C | A | T | – | | | | – | G | A | C | , | C | C | T | –3′ | | – | C | T | G | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCGATT-3′ and 5′-AGGGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 96f1_2bae
| 5′– | T | A | C | , | A | C | C | – | | 3′– | A | T | G | , | T | G | G | – | | | | – | T | T | C | , | T | G | A | , | T | T | G | , | T | G | C | , | G | A | A | , | C | A | C | , | A | T | G | , | A | A | C | – | | – | A | A | G | , | A | C | T | , | A | A | C | , | A | C | G | , | C | T | T | , | G | T | G | , | T | A | C | , | T | T | G | – | | | | – | C | T | A | , | G | G | T | –3′ | | – | G | A | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TACACC-3′ and 5′-ACCTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 46db_1422
| 5′– | G | T | A | , | C | C | T | – | | 3′– | C | A | T | , | G | G | A | – | | | | – | C | A | C | , | G | T | T | , | C | G | A | , | A | T | C | , | T | T | C | , | G | C | T | , | C | G | G | , | T | A | A | – | | – | G | T | G | , | C | A | A | , | G | C | T | , | T | A | G | , | A | A | G | , | C | G | A | , | G | C | C | , | A | T | T | – | | | | – | C | A | A | , | G | A | T | –3′ | | – | G | T | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTACCT-3′ and 5′-ATCTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 6839_2b80
| 5′– | A | C | T | , | T | C | C | – | | 3′– | T | G | A | , | A | G | G | – | | | | – | C | G | C | , | A | G | T | , | A | C | T | , | T | A | C | , | C | C | A | , | A | C | A | , | C | T | A | , | C | G | T | – | | – | G | C | G | , | T | C | A | , | T | G | A | , | A | T | G | , | G | G | T | , | T | G | T | , | G | A | T | , | G | C | A | – | | | | – | A | T | T | , | G | C | C | –3′ | | – | T | A | A | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTTCC-3′ and 5′-GGCAAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 75eb_f298
| 5′– | C | C | A | , | C | G | T | – | | 3′– | G | G | T | , | G | C | A | – | | | | – | G | C | T | , | G | T | A | , | G | C | A | , | A | T | G | , | A | T | G | , | A | T | T | , | G | A | T | , | G | C | A | – | | – | C | G | A | , | C | A | T | , | C | G | T | , | T | A | C | , | T | A | C | , | T | A | A | , | C | T | A | , | C | G | T | – | | | | – | G | A | A | , | G | A | T | –3′ | | – | C | T | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCACGT-3′ and 5′-ATCTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ec2b_2bb9
| 5′– | A | T | G | , | G | T | C | – | | 3′– | T | A | C | , | C | A | G | – | | | | – | C | A | T | , | G | A | G | , | G | A | A | , | A | C | A | , | T | G | A | , | G | A | C | , | T | G | C | , | A | A | T | – | | – | G | T | A | , | C | T | C | , | C | T | T | , | T | G | T | , | A | C | T | , | C | T | G | , | A | C | G | , | T | T | A | – | | | | – | C | T | G | , | G | A | T | –3′ | | – | G | A | C | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGGTC-3′ and 5′-ATCCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 00ef_3d51
| 5′– | C | T | T | , | A | C | T | – | | 3′– | G | A | A | , | T | G | A | – | | | | – | G | A | T | , | C | G | T | , | C | T | T | , | C | A | G | , | T | T | G | , | T | C | G | , | C | A | G | , | A | T | T | – | | – | C | T | A | , | G | C | A | , | G | A | A | , | G | T | C | , | A | A | C | , | A | G | C | , | G | T | C | , | T | A | A | – | | | | – | G | T | C | , | A | C | T | –3′ | | – | C | A | G | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTTACT-3′ and 5′-AGTGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC c8e6_51fa
| 5′– | T | G | A | , | A | A | C | – | | 3′– | A | C | T | , | T | T | G | – | | | | – | T | T | T | , | G | C | A | , | C | T | G | , | G | T | T | , | C | C | C | , | A | C | A | , | G | G | T | , | C | A | C | – | | – | A | A | A | , | C | G | T | , | G | A | C | , | C | A | A | , | G | G | G | , | T | G | T | , | C | C | A | , | G | T | G | – | | | | – | A | T | G | , | C | T | G | –3′ | | – | T | A | C | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAAAC-3′ and 5′-CAGCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 4289_80dd
| 5′– | T | G | A | , | G | G | C | – | | 3′– | A | C | T | , | C | C | G | – | | | | – | G | A | C | , | T | T | T | , | G | G | T | , | C | T | A | , | G | C | T | , | C | A | G | , | A | G | C | , | T | C | T | – | | – | C | T | G | , | A | A | A | , | C | C | A | , | G | A | T | , | C | G | A | , | G | T | C | , | T | C | G | , | A | G | A | – | | | | – | T | C | G | , | A | A | G | –3′ | | – | A | G | C | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAGGC-3′ and 5′-CTTCGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4005_46f0
| 5′– | T | C | A | , | G | G | C | – | | 3′– | A | G | T | , | C | C | G | – | | | | – | A | G | G | , | T | C | T | , | T | T | C | , | T | C | T | , | A | A | G | , | A | T | G | , | A | C | T | , | G | C | C | – | | – | T | C | C | , | A | G | A | , | A | A | G | , | A | G | A | , | T | T | C | , | T | A | C | , | T | G | A | , | C | G | G | – | | | | – | G | A | A | , | G | T | T | –3′ | | – | C | T | T | , | C | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAGGC-3′ and 5′-AACTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5a51_93d4
| 5′– | G | A | A | , | C | G | T | – | | 3′– | C | T | T | , | G | C | A | – | | | | – | T | C | C | , | A | G | A | , | G | T | T | , | A | C | G | , | C | A | T | , | G | T | C | , | A | G | C | , | T | C | C | – | | – | A | G | G | , | T | C | T | , | C | A | A | , | T | G | C | , | G | T | A | , | C | A | G | , | T | C | G | , | A | G | G | – | | | | – | C | A | G | , | T | T | A | –3′ | | – | G | T | C | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GAACGT-3′ and 5′-TAACTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC d300_fe2b
| 5′– | G | A | C | , | T | T | A | – | | 3′– | C | T | G | , | A | A | T | – | | | | – | G | A | G | , | T | C | A | , | C | A | T | , | A | C | A | , | G | C | T | , | A | T | G | , | A | A | A | , | C | G | T | – | | – | C | T | C | , | A | G | T | , | G | T | A | , | T | G | T | , | C | G | A | , | T | A | C | , | T | T | T | , | G | C | A | – | | | | – | A | T | G | , | T | C | C | –3′ | | – | T | A | C | , | A | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACTTA-3′ and 5′-GGACAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a160_43a1
| 5′– | G | G | A | , | A | G | T | – | | 3′– | C | C | T | , | T | C | A | – | | | | – | A | C | T | , | G | A | T | , | T | C | A | , | T | G | G | , | G | T | C | , | A | G | G | , | C | T | T | , | A | G | C | – | | – | T | G | A | , | C | T | A | , | A | G | T | , | A | C | C | , | C | A | G | , | T | C | C | , | G | A | A | , | T | C | G | – | | | | – | C | G | T | , | C | T | A | –3′ | | – | G | C | A | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGAAGT-3′ and 5′-TAGACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 64d1_d652
| 5′– | G | G | T | , | A | G | T | – | | 3′– | C | C | A | , | T | C | A | – | | | | – | T | G | G | , | C | A | C | , | C | T | A | , | G | C | A | , | T | C | C | , | T | C | C | , | A | C | G | , | A | T | G | – | | – | A | C | C | , | G | T | G | , | G | A | T | , | C | G | T | , | A | G | G | , | A | G | G | , | T | G | C | , | T | A | C | – | | | | – | A | C | T | , | G | A | C | –3′ | | – | T | G | A | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGTAGT-3′ and 5′-GTCAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8f64_05f0
| 5′– | C | A | G | , | C | G | T | – | | 3′– | G | T | C | , | G | C | A | – | | | | – | G | A | G | , | C | T | T | , | G | A | C | , | G | T | T | , | G | A | G | , | A | A | T | , | C | A | G | , | T | G | A | – | | – | C | T | C | , | G | A | A | , | C | T | G | , | C | A | A | , | C | T | C | , | T | T | A | , | G | T | C | , | A | C | T | – | | | | – | C | A | A | , | C | T | T | –3′ | | – | G | T | T | , | G | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGCGT-3′ and 5′-AAGTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 3c9e_7024
| 5′– | A | C | T | , | G | T | C | – | | 3′– | T | G | A | , | C | A | G | – | | | | – | T | G | G | , | T | C | A | , | C | T | T | , | T | C | G | , | A | G | C | , | A | A | A | , | G | A | T | , | C | C | G | – | | – | A | C | C | , | A | G | T | , | G | A | A | , | A | G | C | , | T | C | G | , | T | T | T | , | C | T | A | , | G | G | C | – | | | | – | T | C | G | , | T | A | C | –3′ | | – | A | G | C | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTGTC-3′ and 5′-GTACGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 9d85_498a
| 5′– | G | C | A | , | A | G | T | – | | 3′– | C | G | T | , | T | C | A | – | | | | – | G | A | C | , | T | A | G | , | T | G | T | , | T | C | G | , | C | A | A | , | A | C | T | , | G | A | C | , | A | A | T | – | | – | C | T | G | , | A | T | C | , | A | C | A | , | A | G | C | , | G | T | T | , | T | G | A | , | C | T | G | , | T | T | A | – | | | | – | T | C | A | , | T | C | C | –3′ | | – | A | G | T | , | A | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCAAGT-3′ and 5′-GGATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC cbbb_d0d7
| 5′– | C | C | A | , | A | G | T | – | | 3′– | G | G | T | , | T | C | A | – | | | | – | G | A | C | , | G | T | A | , | G | A | A | , | C | T | C | , | G | C | T | , | T | G | G | , | G | T | A | , | G | C | A | – | | – | C | T | G | , | C | A | T | , | C | T | T | , | G | A | G | , | C | G | A | , | A | C | C | , | C | A | T | , | C | G | T | – | | | | – | C | T | G | , | A | G | T | –3′ | | – | G | A | C | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCAAGT-3′ and 5′-ACTCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0afa_6b6a
| 5′– | G | C | T | , | C | C | A | – | | 3′– | C | G | A | , | G | G | T | – | | | | – | C | A | T | , | G | T | G | , | T | G | T | , | G | G | T | , | G | G | T | , | G | T | C | , | T | G | C | , | A | A | A | – | | – | G | T | A | , | C | A | C | , | A | C | A | , | C | C | A | , | C | C | A | , | C | A | G | , | A | C | G | , | T | T | T | – | | | | – | A | C | C | , | A | T | G | –3′ | | – | T | G | G | , | T | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTCCA-3′ and 5′-CATGGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 28b9_fb3c
| 5′– | C | C | A | , | C | A | T | – | | 3′– | G | G | T | , | G | T | A | – | | | | – | G | A | C | , | C | C | T | , | G | A | A | , | C | T | A | , | T | C | A | , | G | A | C | , | G | T | G | , | A | C | T | – | | – | C | T | G | , | G | G | A | , | C | T | T | , | G | A | T | , | A | G | T | , | C | T | G | , | C | A | C | , | T | G | A | – | | | | – | T | T | A | , | C | G | G | –3′ | | – | A | A | T | , | G | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCACAT-3′ and 5′-CCGTAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2b0d_d9c9
| 5′– | C | C | T | , | C | C | A | – | | 3′– | G | G | A | , | G | G | T | – | | | | – | C | T | C | , | A | G | A | , | C | G | A | , | C | T | G | , | C | G | T | , | T | G | A | , | C | A | G | , | T | C | A | – | | – | G | A | G | , | T | C | T | , | G | C | T | , | G | A | C | , | G | C | A | , | A | C | T | , | G | T | C | , | A | G | T | – | | | | – | G | T | T | , | T | G | A | –3′ | | – | C | A | A | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTCCA-3′ and 5′-TCAAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 026a_65ef
| 5′– | C | T | G | , | T | A | A | – | | 3′– | G | A | C | , | A | T | T | – | | | | – | G | T | C | , | T | A | G | , | G | T | T | , | C | A | G | , | G | T | T | , | C | T | G | , | G | A | C | , | A | A | T | – | | – | C | A | G | , | A | T | C | , | C | A | A | , | G | T | C | , | C | A | A | , | G | A | C | , | C | T | G | , | T | T | A | – | | | | – | G | A | A | , | C | A | T | –3′ | | – | C | T | T | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGTAA-3′ and 5′-ATGTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 5fb1_0fc7
| 5′– | G | A | T | , | T | C | A | – | | 3′– | C | T | A | , | A | G | T | – | | | | – | T | G | A | , | G | A | C | , | T | T | C | , | C | C | A | , | A | C | C | , | T | G | T | , | T | A | C | , | T | T | G | – | | – | A | C | T | , | C | T | G | , | A | A | G | , | G | G | T | , | T | G | G | , | A | C | A | , | A | T | G | , | A | A | C | – | | | | – | A | C | C | , | T | G | C | –3′ | | – | T | G | G | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATTCA-3′ and 5′-GCAGGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC d1b2_ae4d
| 5′– | C | G | T | , | T | C | A | – | | 3′– | G | C | A | , | A | G | T | – | | | | – | A | C | T | , | A | T | G | , | T | C | G | , | G | A | T | , | T | C | A | , | T | C | A | , | A | G | G | , | T | C | C | – | | – | T | G | A | , | T | A | C | , | A | G | C | , | C | T | A | , | A | G | T | , | A | G | T | , | T | C | C | , | A | G | G | – | | | | – | A | C | T | , | T | A | G | –3′ | | – | T | G | A | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTTCA-3′ and 5′-CTAAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC c9c2_3492
| 5′– | G | A | T | , | C | G | T | – | | 3′– | C | T | A | , | G | C | A | – | | | | – | A | T | C | , | T | C | G | , | T | T | T | , | C | G | T | , | C | T | C | , | T | C | A | , | C | C | T | , | A | T | G | – | | – | T | A | G | , | A | G | C | , | A | A | A | , | G | C | A | , | G | A | G | , | A | G | T | , | G | G | A | , | T | A | C | – | | | | – | C | A | T | , | A | G | T | –3′ | | – | G | T | A | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATCGT-3′ and 5′-ACTATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b9f8_58b8
| 5′– | C | A | G | , | A | G | T | – | | 3′– | G | T | C | , | T | C | A | – | | | | – | A | G | T | , | C | G | C | , | A | A | A | , | G | T | C | , | G | G | A | , | T | C | C | , | A | C | C | , | T | A | G | – | | – | T | C | A | , | G | C | G | , | T | T | T | , | C | A | G | , | C | C | T | , | A | G | G | , | T | G | G | , | A | T | C | – | | | | – | A | A | G | , | G | T | C | –3′ | | – | T | T | C | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGAGT-3′ and 5′-GACCTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC c29d_2996
| 5′– | C | C | A | , | C | G | T | – | | 3′– | G | G | T | , | G | C | A | – | | | | – | T | A | C | , | A | C | G | , | A | A | T | , | G | T | A | , | C | A | A | , | A | C | A | , | A | G | A | , | A | T | C | – | | – | A | T | G | , | T | G | C | , | T | T | A | , | C | A | T | , | G | T | T | , | T | G | T | , | T | C | T | , | T | A | G | – | | | | – | A | C | T | , | A | G | C | –3′ | | – | T | G | A | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCACGT-3′ and 5′-GCTAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 97e0_da22
| 5′– | T | T | A | , | C | G | G | – | | 3′– | A | A | T | , | G | C | C | – | | | | – | C | G | C | , | T | A | A | , | C | C | C | , | T | G | A | , | C | T | A | , | G | T | A | , | C | A | G | , | C | A | T | – | | – | G | C | G | , | A | T | T | , | G | G | G | , | A | C | T | , | G | A | T | , | C | A | T | , | G | T | C | , | G | T | A | – | | | | – | C | G | A | , | T | G | A | –3′ | | – | G | C | T | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTACGG-3′ and 5′-TCATCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 9c91_28a1
| 5′– | G | A | C | , | T | C | A | – | | 3′– | C | T | G | , | A | G | T | – | | | | – | A | G | A | , | G | C | T | , | C | G | T | , | C | T | G | , | A | T | G | , | T | A | G | , | C | C | A | , | G | T | C | – | | – | T | C | T | , | C | G | A | , | G | C | A | , | G | A | C | , | T | A | C | , | A | T | C | , | G | G | T | , | C | A | G | – | | | | – | C | G | G | , | T | G | A | –3′ | | – | G | C | C | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACTCA-3′ and 5′-TCACCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 081a_1ea9
| 5′– | A | T | C | , | A | G | G | – | | 3′– | T | A | G | , | T | C | C | – | | | | – | A | G | C | , | T | C | A | , | C | A | C | , | T | C | A | , | C | C | T | , | G | A | A | , | C | C | A | , | T | A | G | – | | – | T | C | G | , | A | G | T | , | G | T | G | , | A | G | T | , | G | G | A | , | C | T | T | , | G | G | T | , | A | T | C | – | | | | – | G | G | T | , | G | G | A | –3′ | | – | C | C | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCAGG-3′ and 5′-TCCACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 140f_7601
| 5′– | C | T | A | , | C | G | A | – | | 3′– | G | A | T | , | G | C | T | – | | | | – | A | C | G | , | T | A | G | , | G | C | A | , | C | A | T | , | G | G | A | , | G | C | C | , | A | G | C | , | C | T | C | – | | – | T | G | C | , | A | T | C | , | C | G | T | , | G | T | A | , | C | C | T | , | C | G | G | , | T | C | G | , | G | A | G | – | | | | – | C | T | A | , | C | G | A | –3′ | | – | G | A | T | , | G | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTACGA-3′ and 5′-TCGTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a6d7_efe8
| 5′– | T | C | G | , | A | G | G | – | | 3′– | A | G | C | , | T | C | C | – | | | | – | C | T | G | , | A | A | C | , | C | A | A | , | C | A | T | , | T | C | C | , | T | A | A | , | G | T | T | , | A | C | A | – | | – | G | A | C | , | T | T | G | , | G | T | T | , | G | T | A | , | A | G | G | , | A | T | T | , | C | A | A | , | T | G | T | – | | | | – | G | G | A | , | G | G | T | –3′ | | – | C | C | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCGAGG-3′ and 5′-ACCTCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC b998_0fe9
| 5′– | C | G | T | , | T | G | A | – | | 3′– | G | C | A | , | A | C | T | – | | | | – | T | T | C | , | G | G | A | , | C | C | T | , | T | T | C | , | G | G | A | , | C | A | G | , | T | C | A | , | C | A | G | – | | – | A | A | G | , | C | C | T | , | G | G | A | , | A | A | G | , | C | C | T | , | G | T | C | , | A | G | T | , | G | T | C | – | | | | – | A | A | T | , | C | A | G | –3′ | | – | T | T | A | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTTGA-3′ and 5′-CTGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b965_0acf
| 5′– | C | A | G | , | G | G | T | – | | 3′– | G | T | C | , | C | C | A | – | | | | – | G | A | T | , | C | T | G | , | G | G | T | , | G | T | A | , | A | G | T | , | A | T | C | , | C | G | T | , | A | C | T | – | | – | C | T | A | , | G | A | C | , | C | C | A | , | C | A | T | , | T | C | A | , | T | A | G | , | G | C | A | , | T | G | A | – | | | | – | C | G | G | , | T | C | A | –3′ | | – | G | C | C | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGGGT-3′ and 5′-TGACCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f0fc_de15
| 5′– | G | A | A | , | G | G | T | – | | 3′– | C | T | T | , | C | C | A | – | | | | – | T | C | C | , | A | T | G | , | A | G | C | , | C | T | T | , | A | C | G | , | A | C | G | , | A | G | C | , | G | T | C | – | | – | A | G | G | , | T | A | C | , | T | C | G | , | G | A | A | , | T | G | C | , | T | G | C | , | T | C | G | , | C | A | G | – | | | | – | G | G | T | , | C | T | A | –3′ | | – | C | C | A | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GAAGGT-3′ and 5′-TAGACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 0c01_7fbd
| 5′– | C | A | T | , | T | C | A | – | | 3′– | G | T | A | , | A | G | T | – | | | | – | T | G | A | , | C | G | A | , | A | G | T | , | G | C | A | , | G | T | T | , | G | T | A | , | C | A | T | , | G | A | C | – | | – | A | C | T | , | G | C | T | , | T | C | A | , | C | G | T | , | C | A | A | , | C | A | T | , | G | T | A | , | C | T | G | – | | | | – | G | T | C | , | A | A | T | –3′ | | – | C | A | G | , | T | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATTCA-3′ and 5′-ATTGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 7a7a_660d
| 5′– | G | T | A | , | T | G | A | – | | 3′– | C | A | T | , | A | C | T | – | | | | – | T | G | A | , | C | T | A | , | C | A | A | , | G | C | G | , | T | A | C | , | C | A | G | , | A | C | C | , | G | T | G | – | | – | A | C | T | , | G | A | T | , | G | T | T | , | C | G | C | , | A | T | G | , | G | T | C | , | T | G | G | , | C | A | C | – | | | | – | G | C | T | , | G | A | T | –3′ | | – | C | G | A | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTATGA-3′ and 5′-ATCAGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC d914_e47f
| 5′– | T | T | C | , | T | A | G | – | | 3′– | A | A | G | , | A | T | C | – | | | | – | T | C | C | , | G | A | G | , | A | C | A | , | T | T | G | , | A | T | G | , | T | G | A | , | T | C | G | , | A | G | G | – | | – | A | G | G | , | C | T | C | , | T | G | T | , | A | A | C | , | T | A | C | , | A | C | T | , | A | G | C | , | T | C | C | – | | | | – | G | C | C | , | A | C | T | –3′ | | – | C | G | G | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCTAG-3′ and 5′-AGTGGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC e10d_5c69
| 5′– | G | T | C | , | A | A | T | – | | 3′– | C | A | G | , | T | T | A | – | | | | – | C | T | A | , | G | A | G | , | G | T | T | , | G | C | T | , | T | C | G | , | A | T | G | , | C | A | T | , | G | T | A | – | | – | G | A | T | , | C | T | C | , | C | A | A | , | C | G | A | , | A | G | C | , | T | A | C | , | G | T | A | , | C | A | T | – | | | | – | A | T | T | , | C | A | G | –3′ | | – | T | A | A | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCAAT-3′ and 5′-CTGAAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC b804_9d31
| 5′– | T | T | G | , | A | A | G | – | | 3′– | A | A | C | , | T | T | C | – | | | | – | T | G | C | , | A | G | A | , | A | T | C | , | C | A | T | , | C | A | G | , | G | G | T | , | A | G | A | , | T | A | C | – | | – | A | C | G | , | T | C | T | , | T | A | G | , | G | T | A | , | G | T | C | , | C | C | A | , | T | C | T | , | A | T | G | – | | | | – | C | A | G | , | C | A | T | –3′ | | – | G | T | C | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTGAAG-3′ and 5′-ATGCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC b5ef_1904
| 5′– | T | G | C | , | A | A | G | – | | 3′– | A | C | G | , | T | T | C | – | | | | – | T | T | C | , | G | A | G | , | T | T | A | , | G | A | C | , | C | T | C | , | T | A | A | , | G | C | A | , | T | A | C | – | | – | A | A | G | , | C | T | C | , | A | A | T | , | C | T | G | , | G | A | G | , | A | T | T | , | C | G | T | , | A | T | G | – | | | | – | T | T | C | , | A | T | G | –3′ | | – | A | A | G | , | T | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCAAG-3′ and 5′-CATGAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f201_e2b6
| 5′– | G | G | A | , | T | G | A | – | | 3′– | C | C | T | , | A | C | T | – | | | | – | T | G | C | , | A | T | T | , | G | G | T | , | C | C | A | , | A | G | T | , | A | C | G | , | T | C | C | , | G | A | C | – | | – | A | C | G | , | T | A | A | , | C | C | A | , | G | G | T | , | T | C | A | , | T | G | C | , | A | G | G | , | C | T | G | – | | | | – | C | A | A | , | G | C | T | –3′ | | – | G | T | T | , | C | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGATGA-3′ and 5′-AGCTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9c0a_8132
| 5′– | G | C | T | , | C | A | A | – | | 3′– | C | G | A | , | G | T | T | – | | | | – | G | T | A | , | C | T | A | , | G | G | C | , | T | G | G | , | C | A | G | , | G | G | T | , | T | A | C | , | A | G | A | – | | – | C | A | T | , | G | A | T | , | C | C | G | , | A | C | C | , | G | T | C | , | C | C | A | , | A | T | G | , | T | C | T | – | | | | – | G | A | T | , | G | C | A | –3′ | | – | C | T | A | , | C | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTCAA-3′ and 5′-TGCATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC bbb7_ac02
| 5′– | C | T | G | , | C | A | T | – | | 3′– | G | A | C | , | G | T | A | – | | | | – | C | A | G | , | T | G | A | , | T | A | G | , | T | G | T | , | T | T | C | , | C | T | G | , | C | G | T | , | T | G | A | – | | – | G | T | C | , | A | C | T | , | A | T | C | , | A | C | A | , | A | A | G | , | G | A | C | , | G | C | A | , | A | C | T | – | | | | – | G | A | T | , | A | C | T | –3′ | | – | C | T | A | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGCAT-3′ and 5′-AGTATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC bdaf_b354
| 5′– | C | A | T | , | C | G | A | – | | 3′– | G | T | A | , | G | C | T | – | | | | – | T | C | A | , | C | T | G | , | T | T | A | , | G | A | G | , | A | G | A | , | T | C | A | , | T | A | G | , | A | T | C | – | | – | A | G | T | , | G | A | C | , | A | A | T | , | C | T | C | , | T | C | T | , | A | G | T | , | A | T | C | , | T | A | G | – | | | | – | C | C | T | , | C | T | A | –3′ | | – | G | G | A | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATCGA-3′ and 5′-TAGAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 959f_a442
| 5′– | C | A | A | , | C | T | T | – | | 3′– | G | T | T | , | G | A | A | – | | | | – | A | G | C | , | C | T | A | , | A | C | T | , | A | T | G | , | C | C | A | , | T | C | T | , | C | C | A | , | G | T | C | – | | – | T | C | G | , | G | A | T | , | T | G | A | , | T | A | C | , | G | G | T | , | A | G | A | , | G | G | T | , | C | A | G | – | | | | – | T | T | G | , | C | A | G | –3′ | | – | A | A | C | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAACTT-3′ and 5′-CTGCAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC c834_f343
| 5′– | G | C | T | , | C | T | A | – | | 3′– | C | G | A | , | G | A | T | – | | | | – | C | T | A | , | G | G | C | , | T | C | T | , | C | T | G | , | T | C | C | , | G | T | C | , | T | A | C | , | T | G | T | – | | – | G | A | T | , | C | C | G | , | A | G | A | , | G | A | C | , | A | G | G | , | C | A | G | , | A | T | G | , | A | C | A | – | | | | – | A | A | T | , | G | A | C | –3′ | | – | T | T | A | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTCTA-3′ and 5′-GTCATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 2ae0_78e7
| 5′– | A | G | T | , | G | C | C | – | | 3′– | T | C | A | , | C | G | G | – | | | | – | T | C | C | , | A | G | G | , | T | T | C | , | C | A | G | , | C | T | A | , | G | G | G | , | T | A | G | , | A | C | G | – | | – | A | G | G | , | T | C | C | , | A | A | G | , | G | T | C | , | G | A | T | , | C | C | C | , | A | T | C | , | T | G | C | – | | | | – | G | T | C | , | A | A | T | –3′ | | – | C | A | G | , | T | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTGCC-3′ and 5′-ATTGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC cdd3_0fc1
| 5′– | G | A | T | , | G | G | A | – | | 3′– | C | T | A | , | C | C | T | – | | | | – | T | G | A | , | A | T | C | , | G | A | C | , | C | A | A | , | T | C | T | , | A | C | T | , | C | A | C | , | T | G | G | – | | – | A | C | T | , | T | A | G | , | C | T | G | , | G | T | T | , | A | G | A | , | T | G | A | , | G | T | G | , | A | C | C | – | | | | – | A | A | C | , | T | G | G | –3′ | | – | T | T | G | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATGGA-3′ and 5′-CCAGTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC aa78_bb52
| 5′– | G | A | T | , | C | A | A | – | | 3′– | C | T | A | , | G | T | T | – | | | | – | G | A | C | , | G | T | A | , | C | C | T | , | T | C | A | , | G | C | T | , | A | T | C | , | C | G | A | , | G | T | A | – | | – | C | T | G | , | C | A | T | , | G | G | A | , | A | G | T | , | C | G | A | , | T | A | G | , | G | C | T | , | C | A | T | – | | | | – | C | A | G | , | A | A | T | –3′ | | – | G | T | C | , | T | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATCAA-3′ and 5′-ATTCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC d6f3_1c6c
| 5′– | G | A | C | , | A | T | T | – | | 3′– | C | T | G | , | T | A | A | – | | | | – | C | T | T | , | A | G | C | , | G | A | C | , | T | T | C | , | C | C | T | , | G | G | A | , | T | G | C | , | C | A | A | – | | – | G | A | A | , | T | C | G | , | C | T | G | , | A | A | G | , | G | G | A | , | C | C | T | , | A | C | G | , | G | T | T | – | | | | – | G | A | C | , | C | A | T | –3′ | | – | C | T | G | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACATT-3′ and 5′-ATGGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 7111_2074
| 5′– | T | G | A | , | A | G | G | – | | 3′– | A | C | T | , | T | C | C | – | | | | – | G | T | C | , | C | C | A | , | G | G | C | , | A | T | C | , | T | T | G | , | C | T | G | , | C | T | G | , | T | T | A | – | | – | C | A | G | , | G | G | T | , | C | C | G | , | T | A | G | , | A | A | C | , | G | A | C | , | G | A | C | , | A | A | T | – | | | | – | A | G | G | , | T | T | G | –3′ | | – | T | C | C | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAAGG-3′ and 5′-CAACCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 6029_3ce1
| 5′– | A | G | G | , | T | G | G | – | | 3′– | T | C | C | , | A | C | C | – | | | | – | G | A | A | , | G | C | T | , | T | C | T | , | T | C | A | , | A | G | G | , | T | C | T | , | G | T | A | , | C | T | T | – | | – | C | T | T | , | C | G | A | , | A | G | A | , | A | G | T | , | T | C | C | , | A | G | A | , | C | A | T | , | G | A | A | – | | | | – | T | G | G | , | A | T | G | –3′ | | – | A | C | C | , | T | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTGG-3′ and 5′-CATCCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 300c_9dc1
| 5′– | A | G | T | , | A | A | C | – | | 3′– | T | C | A | , | T | T | G | – | | | | – | G | C | A | , | C | T | T | , | C | C | A | , | G | G | C | , | T | T | C | , | A | A | T | , | G | A | A | , | G | C | T | – | | – | C | G | T | , | G | A | A | , | G | G | T | , | C | C | G | , | A | A | G | , | T | T | A | , | C | T | T | , | C | G | A | – | | | | – | T | A | G | , | G | T | C | –3′ | | – | A | T | C | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTAAC-3′ and 5′-GACCTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC dfa7_dad6
| 5′– | A | A | C | , | G | T | C | – | | 3′– | T | T | G | , | C | A | G | – | | | | – | A | A | T | , | G | C | C | , | A | G | T | , | T | T | G | , | G | T | T | , | C | A | A | , | T | G | C | , | A | A | G | – | | – | T | T | A | , | C | G | G | , | T | C | A | , | A | A | C | , | C | A | A | , | G | T | T | , | A | C | G | , | T | T | C | – | | | | – | T | G | C | , | T | A | G | –3′ | | – | A | C | G | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACGTC-3′ and 5′-CTAGCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC cf66_9a69
| 5′– | C | T | T | , | C | T | A | – | | 3′– | G | A | A | , | G | A | T | – | | | | – | T | A | C | , | C | G | T | , | A | C | T | , | T | A | C | , | G | A | C | , | T | G | T | , | C | T | T | , | C | A | G | – | | – | A | T | G | , | G | C | A | , | T | G | A | , | A | T | G | , | C | T | G | , | A | C | A | , | G | A | A | , | G | T | C | – | | | | – | C | T | A | , | G | T | T | –3′ | | – | G | A | T | , | C | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTTCTA-3′ and 5′-AACTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 06b1_e249
| 5′– | A | T | C | , | T | C | G | – | | 3′– | T | A | G | , | A | G | C | – | | | | – | C | A | C | , | T | G | G | , | A | G | G | , | T | T | C | , | T | C | A | , | C | T | C | , | G | T | T | , | C | T | A | – | | – | G | T | G | , | A | C | C | , | T | C | C | , | A | A | G | , | A | G | T | , | G | A | G | , | C | A | A | , | G | A | T | – | | | | – | T | C | A | , | T | T | C | –3′ | | – | A | G | T | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCTCG-3′ and 5′-GAATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 95de_1be3
| 5′– | A | G | C | , | G | T | C | – | | 3′– | T | C | G | , | C | A | G | – | | | | – | G | C | C | , | T | A | C | , | T | C | A | , | A | T | C | , | G | G | T | , | A | G | A | , | A | G | C | , | G | T | A | – | | – | C | G | G | , | A | T | G | , | A | G | T | , | T | A | G | , | C | C | A | , | T | C | T | , | T | C | G | , | C | A | T | – | | | | – | C | A | T | , | G | A | A | –3′ | | – | G | T | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGCGTC-3′ and 5′-TTCATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 15a9_4e84
| 5′– | A | C | T | , | A | A | G | – | | 3′– | T | G | A | , | T | T | C | – | | | | – | G | C | T | , | T | G | A | , | T | C | T | , | G | G | T | , | C | T | G | , | G | A | G | , | T | A | C | , | G | G | T | – | | – | C | G | A | , | A | C | T | , | A | G | A | , | C | C | A | , | G | A | C | , | C | T | C | , | A | T | G | , | C | C | A | – | | | | – | C | C | G | , | T | C | A | –3′ | | – | G | G | C | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTAAG-3′ and 5′-TGACGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 09da_9d67
| 5′– | A | T | C | , | A | G | G | – | | 3′– | T | A | G | , | T | C | C | – | | | | – | C | A | A | , | G | C | T | , | T | G | T | , | A | C | G | , | G | T | T | , | C | T | G | , | A | C | G | , | T | G | A | – | | – | G | T | T | , | C | G | A | , | A | C | A | , | T | G | C | , | C | A | A | , | G | A | C | , | T | G | C | , | A | C | T | – | | | | – | C | A | A | , | A | C | T | –3′ | | – | G | T | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCAGG-3′ and 5′-AGTTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 770d_55c9
| 5′– | G | T | A | , | C | T | A | – | | 3′– | C | A | T | , | G | A | T | – | | | | – | T | G | C | , | A | A | G | , | T | G | T | , | G | C | G | , | A | T | G | , | T | C | A | , | C | G | G | , | A | T | G | – | | – | A | C | G | , | T | T | C | , | A | C | A | , | C | G | C | , | T | A | C | , | A | G | T | , | G | C | C | , | T | A | C | – | | | | – | A | C | C | , | T | A | C | –3′ | | – | T | G | G | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTACTA-3′ and 5′-GTAGGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 6030_11d7
| 5′– | G | C | A | , | G | A | T | – | | 3′– | C | G | T | , | C | T | A | – | | | | – | C | G | A | , | C | T | G | , | C | A | A | , | C | G | T | , | G | T | A | , | T | G | T | , | A | C | T | , | G | G | A | – | | – | G | C | T | , | G | A | C | , | G | T | T | , | G | C | A | , | C | A | T | , | A | C | A | , | T | G | A | , | C | C | T | – | | | | – | A | C | T | , | G | C | C | –3′ | | – | T | G | A | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCAGAT-3′ and 5′-GGCAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 9a0d_8801
| 5′– | T | A | C | , | C | A | G | – | | 3′– | A | T | G | , | G | T | C | – | | | | – | A | C | T | , | T | T | G | , | T | G | G | , | C | T | C | , | G | A | T | , | C | A | A | , | G | A | T | , | C | A | G | – | | – | T | G | A | , | A | A | C | , | A | C | C | , | G | A | G | , | C | T | A | , | G | T | T | , | C | T | A | , | G | T | C | – | | | | – | A | C | G | , | A | T | C | –3′ | | – | T | G | C | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TACCAG-3′ and 5′-GATCGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0835_5d16
| 5′– | A | C | T | , | T | C | C | – | | 3′– | T | G | A | , | A | G | G | – | | | | – | T | A | C | , | G | T | C | , | T | A | T | , | C | T | C | , | T | G | C | , | T | G | G | , | T | C | T | , | A | C | G | – | | – | A | T | G | , | C | A | G | , | A | T | A | , | G | A | G | , | A | C | G | , | A | C | C | , | A | G | A | , | T | G | C | – | | | | – | T | T | G | , | A | G | G | –3′ | | – | A | A | C | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTTCC-3′ and 5′-CCTCAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5642_bfad
| 5′– | C | A | A | , | G | G | T | – | | 3′– | G | T | T | , | C | C | A | – | | | | – | T | G | T | , | T | A | C | , | C | G | T | , | A | T | G | , | A | C | C | , | A | T | T | , | G | A | A | , | C | T | G | – | | – | A | C | A | , | A | T | G | , | G | C | A | , | T | A | C | , | T | G | G | , | T | A | A | , | C | T | T | , | G | A | C | – | | | | – | C | T | A | , | G | T | T | –3′ | | – | G | A | T | , | C | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAAGGT-3′ and 5′-AACTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC dc08_3e70
| 5′– | T | G | A | , | C | G | G | – | | 3′– | A | C | T | , | G | C | C | – | | | | – | A | C | G | , | T | A | A | , | G | G | A | , | A | A | G | , | A | G | A | , | G | G | T | , | C | A | T | , | G | A | G | – | | – | T | G | C | , | A | T | T | , | C | C | T | , | T | T | C | , | T | C | T | , | C | C | A | , | G | T | A | , | C | T | C | – | | | | – | C | T | T | , | C | T | A | –3′ | | – | G | A | A | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGACGG-3′ and 5′-TAGAAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 6124_e11c
| 5′– | T | G | A | , | G | C | C | – | | 3′– | A | C | T | , | C | G | G | – | | | | – | T | A | T | , | G | C | A | , | A | A | C | , | C | T | T | , | A | G | A | , | G | C | T | , | A | G | G | , | C | T | C | – | | – | A | T | A | , | C | G | T | , | T | T | G | , | G | A | A | , | T | C | T | , | C | G | A | , | T | C | C | , | G | A | G | – | | | | – | T | T | A | , | G | A | C | –3′ | | – | A | A | T | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAGCC-3′ and 5′-GTCTAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC d87d_983a
| 5′– | A | A | C | , | T | C | G | – | | 3′– | T | T | G | , | A | G | C | – | | | | – | C | T | A | , | C | T | G | , | T | T | G | , | C | A | C | , | G | A | A | , | G | T | T | , | C | G | C | , | A | T | A | – | | – | G | A | T | , | G | A | C | , | A | A | C | , | G | T | G | , | C | T | T | , | C | A | A | , | G | C | G | , | T | A | T | – | | | | – | G | T | C | , | C | A | A | –3′ | | – | C | A | G | , | G | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACTCG-3′ and 5′-TTGGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 25ff_49c8
| 5′– | G | T | A | , | C | G | A | – | | 3′– | C | A | T | , | G | C | T | – | | | | – | G | C | T | , | T | A | C | , | G | T | A | , | A | C | A | , | G | G | C | , | T | A | T | , | G | T | A | , | T | C | A | – | | – | C | G | A | , | A | T | G | , | C | A | T | , | T | G | T | , | C | C | G | , | A | T | A | , | C | A | T | , | A | G | T | – | | | | – | A | A | T | , | C | T | G | –3′ | | – | T | T | A | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTACGA-3′ and 5′-CAGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c849_c771
| 5′– | T | G | A | , | C | A | G | – | | 3′– | A | C | T | , | G | T | C | – | | | | – | T | G | T | , | C | T | A | , | C | T | C | , | A | T | T | , | C | A | G | , | G | T | T | , | G | T | A | , | G | C | C | – | | – | A | C | A | , | G | A | T | , | G | A | G | , | T | A | A | , | G | T | C | , | C | A | A | , | C | A | T | , | C | G | G | – | | | | – | G | A | T | , | C | G | T | –3′ | | – | C | T | A | , | G | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGACAG-3′ and 5′-ACGATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7b35_09d0
| 5′– | A | T | G | , | A | C | C | – | | 3′– | T | A | C | , | T | G | G | – | | | | – | C | A | C | , | G | G | T | , | A | G | G | , | C | T | A | , | T | G | A | , | G | T | C | , | C | A | A | , | T | G | T | – | | – | G | T | G | , | C | C | A | , | T | C | C | , | G | A | T | , | A | C | T | , | C | A | G | , | G | T | T | , | A | C | A | – | | | | – | C | C | T | , | G | A | A | –3′ | | – | G | G | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGACC-3′ and 5′-TTCAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC af0c_f99a
| 5′– | A | C | T | , | A | A | G | – | | 3′– | T | G | A | , | T | T | C | – | | | | – | A | G | C | , | A | T | C | , | C | C | T | , | G | T | T | , | G | T | T | , | C | G | G | , | T | A | C | , | T | T | G | – | | – | T | C | G | , | T | A | G | , | G | G | A | , | C | A | A | , | C | A | A | , | G | C | C | , | A | T | G | , | A | A | C | – | | | | – | T | C | G | , | A | A | G | –3′ | | – | A | G | C | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTAAG-3′ and 5′-CTTCGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 73a6_c7b0
| 5′– | G | A | A | , | T | G | A | – | | 3′– | C | T | T | , | A | C | T | – | | | | – | G | A | C | , | T | T | G | , | G | A | C | , | C | A | G | , | A | T | C | , | C | C | T | , | C | C | C | , | A | G | T | – | | – | C | T | G | , | A | A | C | , | C | T | G | , | G | T | C | , | T | A | G | , | G | G | A | , | G | G | G | , | T | C | A | – | | | | – | T | T | A | , | G | C | C | –3′ | | – | A | A | T | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GAATGA-3′ and 5′-GGCTAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC babd_444a
| 5′– | T | A | G | , | C | T | G | – | | 3′– | A | T | C | , | G | A | C | – | | | | – | T | G | C | , | T | A | T | , | C | A | T | , | A | C | A | , | A | T | C | , | C | T | T | , | C | A | G | , | T | C | C | – | | – | A | C | G | , | A | T | A | , | G | T | A | , | T | G | T | , | T | A | G | , | G | A | A | , | G | T | C | , | A | G | G | – | | | | – | C | T | A | , | C | C | T | –3′ | | – | G | A | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGCTG-3′ and 5′-AGGTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC eceb_2be1
| 5′– | A | A | C | , | T | A | C | – | | 3′– | T | T | G | , | A | T | G | – | | | | – | G | A | C | , | T | A | G | , | G | T | G | , | T | C | A | , | T | T | G | , | T | C | T | , | G | C | A | , | C | C | T | – | | – | C | T | G | , | A | T | C | , | C | A | C | , | A | G | T | , | A | A | C | , | A | G | A | , | C | G | T | , | G | G | A | – | | | | – | A | A | T | , | G | G | C | –3′ | | – | T | T | A | , | C | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACTAC-3′ and 5′-GCCATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 478e_1cba
| 5′– | A | T | G | , | T | G | G | – | | 3′– | T | A | C | , | A | C | C | – | | | | – | G | G | T | , | A | A | C | , | A | G | C | , | A | T | T | , | G | C | A | , | C | T | T | , | G | A | A | , | A | C | T | – | | – | C | C | A | , | T | T | G | , | T | C | G | , | T | A | A | , | C | G | T | , | G | A | A | , | C | T | T | , | T | G | A | – | | | | – | A | C | T | , | T | T | G | –3′ | | – | T | G | A | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGTGG-3′ and 5′-CAAAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 6b70_7085
| 5′– | T | A | G | , | T | G | C | – | | 3′– | A | T | C | , | A | C | G | – | | | | – | C | C | C | , | A | T | G | , | C | A | T | , | C | A | A | , | A | G | G | , | G | A | C | , | T | T | G | , | C | G | A | – | | – | G | G | G | , | T | A | C | , | G | T | A | , | G | T | T | , | T | C | C | , | C | T | G | , | A | A | C | , | G | C | T | – | | | | – | C | A | G | , | A | G | T | –3′ | | – | G | T | C | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGTGC-3′ and 5′-ACTCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 0404_29fd
| 5′– | C | G | A | , | G | T | T | – | | 3′– | G | C | T | , | C | A | A | – | | | | – | A | G | C | , | T | A | T | , | C | C | A | , | C | A | C | , | A | G | T | , | A | C | C | , | C | T | A | , | A | C | G | – | | – | T | C | G | , | A | T | A | , | G | G | T | , | G | T | G | , | T | C | A | , | T | G | G | , | G | A | T | , | T | G | C | – | | | | – | C | C | T | , | A | C | T | –3′ | | – | G | G | A | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGAGTT-3′ and 5′-AGTAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 12dd_a970
| 5′– | G | C | T | , | T | C | A | – | | 3′– | C | G | A | , | A | G | T | – | | | | – | A | G | C | , | C | T | C | , | A | A | C | , | T | G | A | , | C | T | T | , | G | A | A | , | T | C | T | , | A | T | G | – | | – | T | C | G | , | G | A | G | , | T | T | G | , | A | C | T | , | G | A | A | , | C | T | T | , | A | G | A | , | T | A | C | – | | | | – | C | T | A | , | C | C | A | –3′ | | – | G | A | T | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTTCA-3′ and 5′-TGGTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 3638_b5e1
| 5′– | G | A | C | , | T | T | A | – | | 3′– | C | T | G | , | A | A | T | – | | | | – | G | C | A | , | C | C | T | , | T | A | G | , | T | T | T | , | C | T | A | , | T | C | A | , | A | A | G | , | A | C | T | – | | – | C | G | T | , | G | G | A | , | A | T | C | , | A | A | A | , | G | A | T | , | A | G | T | , | T | T | C | , | T | G | A | – | | | | – | C | A | A | , | T | C | A | –3′ | | – | G | T | T | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACTTA-3′ and 5′-TGATTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 8129_a3e9
| 5′– | C | A | G | , | A | C | T | – | | 3′– | G | T | C | , | T | G | A | – | | | | – | T | G | A | , | G | C | T | , | G | G | T | , | T | C | C | , | A | G | A | , | C | C | C | , | T | A | C | , | G | A | G | – | | – | A | C | T | , | C | G | A | , | C | C | A | , | A | G | G | , | T | C | T | , | G | G | G | , | A | T | G | , | C | T | C | – | | | | – | G | A | C | , | A | C | T | –3′ | | – | C | T | G | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGACT-3′ and 5′-AGTGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a253_a62d
| 5′– | C | A | T | , | C | G | A | – | | 3′– | G | T | A | , | G | C | T | – | | | | – | T | C | A | , | G | G | T | , | T | C | A | , | G | T | T | , | A | G | A | , | G | A | G | , | A | T | T | , | G | G | C | – | | – | A | G | T | , | C | C | A | , | A | G | T | , | C | A | A | , | T | C | T | , | C | T | C | , | T | A | A | , | C | C | G | – | | | | – | A | A | G | , | T | A | G | –3′ | | – | T | T | C | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATCGA-3′ and 5′-CTACTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5378_c56e
| 5′– | G | T | A | , | C | C | A | – | | 3′– | C | A | T | , | G | G | T | – | | | | – | G | A | G | , | A | T | C | , | A | A | G | , | G | A | G | , | T | C | C | , | A | G | C | , | A | G | C | , | T | C | A | – | | – | C | T | C | , | T | A | G | , | T | T | C | , | C | T | C | , | A | G | G | , | T | C | G | , | T | C | G | , | A | G | T | – | | | | – | G | T | A | , | C | T | T | –3′ | | – | C | A | T | , | G | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTACCA-3′ and 5′-AAGTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 4baa_3bbf
| 5′– | G | G | T | , | G | A | A | – | | 3′– | C | C | A | , | C | T | T | – | | | | – | G | T | C | , | A | G | A | , | G | A | A | , | C | C | G | , | A | A | A | , | G | C | T | , | C | G | T | , | A | C | A | – | | – | C | A | G | , | T | C | T | , | C | T | T | , | G | G | C | , | T | T | T | , | C | G | A | , | G | C | A | , | T | G | T | – | | | | – | G | T | C | , | C | A | A | –3′ | | – | C | A | G | , | G | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGTGAA-3′ and 5′-TTGGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC f528_eae2
| 5′– | G | A | C | , | C | T | T | – | | 3′– | C | T | G | , | G | A | A | – | | | | – | A | T | C | , | T | G | T | , | G | T | C | , | G | T | G | , | G | C | T | , | C | G | T | , | A | T | G | , | T | C | C | – | | – | T | A | G | , | A | C | A | , | C | A | G | , | C | A | C | , | C | G | A | , | G | C | A | , | T | A | C | , | A | G | G | – | | | | – | G | A | A | , | G | A | T | –3′ | | – | C | T | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACCTT-3′ and 5′-ATCTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 76db_0b53
| 5′– | C | C | A | , | C | G | T | – | | 3′– | G | G | T | , | G | C | A | – | | | | – | T | G | A | , | A | A | C | , | C | C | A | , | G | T | C | , | A | C | A | , | T | G | T | , | C | G | C | , | A | T | G | – | | – | A | C | T | , | T | T | G | , | G | G | T | , | C | A | G | , | T | G | T | , | A | C | A | , | G | C | G | , | T | A | C | – | | | | – | C | T | G | , | C | T | A | –3′ | | – | G | A | C | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCACGT-3′ and 5′-TAGCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC e21f_45fc
| 5′– | C | A | T | , | G | T | T | – | | 3′– | G | T | A | , | C | A | A | – | | | | – | G | T | G | , | A | C | T | , | C | G | C | , | T | C | A | , | T | C | A | , | C | G | A | , | G | G | A | , | A | C | T | – | | – | C | A | C | , | T | G | A | , | G | C | G | , | A | G | T | , | A | G | T | , | G | C | T | , | C | C | T | , | T | G | A | – | | | | – | T | A | G | , | C | T | G | –3′ | | – | A | T | C | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATGTT-3′ and 5′-CAGCTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8c18_a977
| 5′– | G | A | C | , | G | C | T | – | | 3′– | C | T | G | , | C | G | A | – | | | | – | G | T | C | , | G | A | A | , | C | A | A | , | C | G | T | , | C | A | A | , | A | G | A | , | C | G | C | , | T | T | A | – | | – | C | A | G | , | C | T | T | , | G | T | T | , | G | C | A | , | G | T | T | , | T | C | T | , | G | C | G | , | A | A | T | – | | | | – | T | A | A | , | C | T | G | –3′ | | – | A | T | T | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACGCT-3′ and 5′-CAGTTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2271_d497
| 5′– | T | C | G | , | A | G | G | – | | 3′– | A | G | C | , | T | C | C | – | | | | – | T | G | A | , | T | T | C | , | A | A | G | , | T | G | A | , | A | C | G | , | A | T | C | , | C | C | T | , | G | A | G | – | | – | A | C | T | , | A | A | G | , | T | T | C | , | A | C | T | , | T | G | C | , | T | A | G | , | G | G | A | , | C | T | C | – | | | | – | A | C | G | , | T | T | G | –3′ | | – | T | G | C | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCGAGG-3′ and 5′-CAACGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 5530_605b
| 5′– | A | T | G | , | A | G | G | – | | 3′– | T | A | C | , | T | C | C | – | | | | – | A | G | C | , | T | C | A | , | G | G | A | , | C | A | T | , | T | C | T | , | C | A | A | , | C | G | T | , | A | A | G | – | | – | T | C | G | , | A | G | T | , | C | C | T | , | G | T | A | , | A | G | A | , | G | T | T | , | G | C | A | , | T | T | C | – | | | | – | G | C | T | , | C | A | A | –3′ | | – | C | G | A | , | G | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGAGG-3′ and 5′-TTGAGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 92f3_d49f
| 5′– | T | C | A | , | A | C | G | – | | 3′– | A | G | T | , | T | G | C | – | | | | – | T | A | C | , | G | G | T | , | G | G | A | , | C | A | C | , | A | T | C | , | C | T | A | , | T | C | C | , | A | A | G | – | | – | A | T | G | , | C | C | A | , | C | C | T | , | G | T | G | , | T | A | G | , | G | A | T | , | A | G | G | , | T | T | C | – | | | | – | C | A | A | , | C | T | T | –3′ | | – | G | T | T | , | G | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAACG-3′ and 5′-AAGTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 9f4f_e407
| 5′– | G | A | C | , | C | A | T | – | | 3′– | C | T | G | , | G | T | A | – | | | | – | T | G | A | , | A | C | C | , | A | A | A | , | C | C | T | , | T | G | C | , | A | T | G | , | C | T | A | , | G | A | C | – | | – | A | C | T | , | T | G | G | , | T | T | T | , | G | G | A | , | A | C | G | , | T | A | C | , | G | A | T | , | C | T | G | – | | | | – | G | C | T | , | G | C | A | –3′ | | – | C | G | A | , | C | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACCAT-3′ and 5′-TGCAGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC aeb9_d417
| 5′– | A | C | T | , | A | G | G | – | | 3′– | T | G | A | , | T | C | C | – | | | | – | C | C | C | , | T | A | G | , | C | T | C | , | T | C | G | , | G | A | G | , | G | G | T | , | C | G | C | , | A | T | A | – | | – | G | G | G | , | A | T | C | , | G | A | G | , | A | G | C | , | C | T | C | , | C | C | A | , | G | C | G | , | T | A | T | – | | | | – | A | T | G | , | A | G | C | –3′ | | – | T | A | C | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTAGG-3′ and 5′-GCTCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC a332_01c8
| 5′– | G | G | C | , | A | C | T | – | | 3′– | C | C | G | , | T | G | A | – | | | | – | C | G | A | , | A | T | C | , | A | C | T | , | C | C | C | , | A | C | C | , | C | T | A | , | G | G | A | , | C | G | T | – | | – | G | C | T | , | T | A | G | , | T | G | A | , | G | G | G | , | T | G | G | , | G | A | T | , | C | C | T | , | G | C | A | – | | | | – | G | G | T | , | G | G | A | –3′ | | – | C | C | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGCACT-3′ and 5′-TCCACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 7082_8d37
| 5′– | T | G | C | , | A | C | C | – | | 3′– | A | C | G | , | T | G | G | – | | | | – | C | G | T | , | C | A | G | , | A | C | A | , | T | C | T | , | C | T | G | , | T | T | T | , | C | G | A | , | G | A | T | – | | – | G | C | A | , | G | T | C | , | T | G | T | , | A | G | A | , | G | A | C | , | A | A | A | , | G | C | T | , | C | T | A | – | | | | – | C | A | G | , | G | C | T | –3′ | | – | G | T | C | , | C | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCACC-3′ and 5′-AGCCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC c099_ab1b
| 5′– | A | A | C | , | T | G | G | – | | 3′– | T | T | G | , | A | C | C | – | | | | – | G | T | A | , | C | A | T | , | A | C | T | , | G | A | A | , | T | G | G | , | T | A | C | , | C | A | A | , | C | G | T | – | | – | C | A | T | , | G | T | A | , | T | G | A | , | C | T | T | , | A | C | C | , | A | T | G | , | G | T | T | , | G | C | A | – | | | | – | A | T | C | , | T | T | C | –3′ | | – | T | A | G | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACTGG-3′ and 5′-GAAGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC e996_7081
| 5′– | T | G | A | , | T | G | G | – | | 3′– | A | C | T | , | A | C | C | – | | | | – | C | A | G | , | T | C | G | , | C | A | G | , | G | A | G | , | G | C | A | , | T | T | G | , | A | G | C | , | C | A | T | – | | – | G | T | C | , | A | G | C | , | G | T | C | , | C | T | C | , | C | G | T | , | A | A | C | , | T | C | G | , | G | T | A | – | | | | – | A | C | T | , | C | G | G | –3′ | | – | T | G | A | , | G | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGATGG-3′ and 5′-CCGAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 9c49_aa9b
| 5′– | T | G | C | , | A | C | C | – | | 3′– | A | C | G | , | T | G | G | – | | | | – | C | A | C | , | A | G | T | , | A | C | A | , | A | G | G | , | A | C | T | , | T | T | G | , | A | G | A | , | G | C | T | – | | – | G | T | G | , | T | C | A | , | T | G | T | , | T | C | C | , | T | G | A | , | A | A | C | , | T | C | T | , | C | G | A | – | | | | – | A | A | G | , | A | T | G | –3′ | | – | T | T | C | , | T | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCACC-3′ and 5′-CATCTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 3fbc_217c
| 5′– | A | A | C | , | C | T | G | – | | 3′– | T | T | G | , | G | A | C | – | | | | – | A | T | A | , | G | C | C | , | A | C | C | , | C | T | G | , | A | T | G | , | A | T | G | , | G | C | A | , | T | A | C | – | | – | T | A | T | , | C | G | G | , | T | G | G | , | G | A | C | , | T | A | C | , | T | A | C | , | C | G | T | , | A | T | G | – | | | | – | G | C | A | , | G | A | T | –3′ | | – | C | G | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACCTG-3′ and 5′-ATCTGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 146e_a1ae
| 5′– | G | T | A | , | C | A | A | – | | 3′– | C | A | T | , | G | T | T | – | | | | – | A | G | C | , | A | G | T | , | G | T | G | , | G | A | T | , | G | A | G | , | G | C | A | , | A | T | C | , | A | C | G | – | | – | T | C | G | , | T | C | A | , | C | A | C | , | C | T | A | , | C | T | C | , | C | G | T | , | T | A | G | , | T | G | C | – | | | | – | A | T | G | , | T | T | C | –3′ | | – | T | A | C | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTACAA-3′ and 5′-GAACAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 49b1_1608
| 5′– | C | C | A | , | G | A | T | – | | 3′– | G | G | T | , | C | T | A | – | | | | – | T | C | T | , | T | A | G | , | C | A | T | , | A | C | G | , | T | T | C | , | C | A | G | , | G | C | T | , | A | T | G | – | | – | A | G | A | , | A | T | C | , | G | T | A | , | T | G | C | , | A | A | G | , | G | T | C | , | C | G | A | , | T | A | C | – | | | | – | A | T | C | , | A | G | G | –3′ | | – | T | A | G | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCAGAT-3′ and 5′-CCTGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC f595_a608
| 5′– | A | T | T | , | C | G | G | – | | 3′– | T | A | A | , | G | C | C | – | | | | – | A | T | T | , | G | A | C | , | G | C | A | , | C | A | G | , | C | C | A | , | A | C | G | , | A | C | A | , | T | C | G | – | | – | T | A | A | , | C | T | G | , | C | G | T | , | G | T | C | , | G | G | T | , | T | G | C | , | T | G | T | , | A | G | C | – | | | | – | C | G | T | , | C | G | A | –3′ | | – | G | C | A | , | G | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATTCGG-3′ and 5′-TCGACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect