MC

a613_12df

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Tropomyosin (Trop)5.135.0
Trypsin (Tryp)10.823.5

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Trop and Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Trop and Tryp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Trop will have a positive (+) charge and travel towards the negative (–) terminal
Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Trop will have a negative (–) charge and travel towards the positive (+) terminal
Tryp will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

8f02_3986

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Fumerase (Fum)7.648.5
Histone (His)10.815.0

Both protein samples are placed into a gel with a constant pH of 9.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.0?

Both Fum and His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Fum and His will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Fum will have a positive (+) charge and travel towards the negative (–) terminal
His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Fum will have a negative (–) charge and travel towards the positive (+) terminal
His will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

658c_1894

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Immunoglobulin (IgG)7.3145.0
Leghemoglobin (Leg)4.716.0

Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.5?

Both IgG and Leg will have a negative (–) charge and travel towards the positive (+) terminal Correct Both IgG and Leg will have a positive (+) charge and travel towards the negative (–) terminal Incorrect IgG will have a positive (+) charge and travel towards the negative (–) terminal
Leg will have a negative (–) charge and travel towards the positive (+) terminal Incorrect IgG will have a negative (–) charge and travel towards the positive (+) terminal
Leg will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

79f4_36c4

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Serum Albumin (Alb)4.966.2
Fumerase (Fum)7.648.5

Both protein samples are placed into a gel with a constant pH of 9.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.0?

Both Alb and Fum will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Alb and Fum will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Alb will have a positive (+) charge and travel towards the negative (–) terminal
Fum will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Alb will have a negative (–) charge and travel towards the positive (+) terminal
Fum will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

8788_26e8

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Cytochrome c (Cyt)10.213.0
Xylosidase (Xyl)5.0100.0

Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 11.5?

Both Cyt and Xyl will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Cyt and Xyl will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Cyt will have a positive (+) charge and travel towards the negative (–) terminal
Xyl will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cyt will have a negative (–) charge and travel towards the positive (+) terminal
Xyl will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

f742_de92

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Transferrin (Tran)5.980.0
G3P Dehydrogenase (GDH)8.336.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Tran and GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Tran and GDH will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Tran will have a positive (+) charge and travel towards the negative (–) terminal
GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Tran will have a negative (–) charge and travel towards the positive (+) terminal
GDH will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

0223_2149

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
DNA Ligase (Lig)6.060.0
Serine Protease (Ser)4.122.0

Both protein samples are placed into a gel with a constant pH of 5.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.0?

Both Lig and Ser will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lig and Ser will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lig will have a positive (+) charge and travel towards the negative (–) terminal
Ser will have a negative (–) charge and travel towards the positive (+) terminal Correct Lig will have a negative (–) charge and travel towards the positive (+) terminal
Ser will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

732c_70fc

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Urease A (Ure)4.926.5
Pepsin (Pep)1.034.5

Both protein samples are placed into a gel with a constant pH of 3.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.0?

Both Ure and Pep will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ure and Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ure will have a positive (+) charge and travel towards the negative (–) terminal
Pep will have a negative (–) charge and travel towards the positive (+) terminal Correct Ure will have a negative (–) charge and travel towards the positive (+) terminal
Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

2bfe_727a

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Myoglobin (Myo)7.018.0
Actin (Act)5.343.0

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Myo and Act will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Myo and Act will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Myo will have a positive (+) charge and travel towards the negative (–) terminal
Act will have a negative (–) charge and travel towards the positive (+) terminal Correct Myo will have a negative (–) charge and travel towards the positive (+) terminal
Act will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

606c_666e

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Salmine (Sal)12.13.0
Avidin (Av)10.516.9

Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 11.5?

Both Sal and Av will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Sal and Av will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Sal will have a positive (+) charge and travel towards the negative (–) terminal
Av will have a negative (–) charge and travel towards the positive (+) terminal Correct Sal will have a negative (–) charge and travel towards the positive (+) terminal
Av will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

ed78_0ca7

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lipase (Lip)4.740.0
γ-Globulin (Glob)6.657.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Lip and Glob will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lip and Glob will have a positive (+) charge and travel towards the negative (–) terminal Correct Lip will have a positive (+) charge and travel towards the negative (–) terminal
Glob will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lip will have a negative (–) charge and travel towards the positive (+) terminal
Glob will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

f04f_d176

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lipase (Lip)4.740.0
Aprotinin (Apr)9.26.5

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Lip and Apr will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lip and Apr will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lip will have a positive (+) charge and travel towards the negative (–) terminal
Apr will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lip will have a negative (–) charge and travel towards the positive (+) terminal
Apr will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

4a5c_77cb

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Pepsin (Pep)1.034.5
Enolase (Eno)8.442.5

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both Pep and Eno will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Pep and Eno will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Pep will have a positive (+) charge and travel towards the negative (–) terminal
Eno will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Pep will have a negative (–) charge and travel towards the positive (+) terminal
Eno will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

5cc9_ef99

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Avidin (Av)10.516.9
Transferrin (Tran)5.980.0

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both Av and Tran will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Av and Tran will have a positive (+) charge and travel towards the negative (–) terminal Correct Av will have a positive (+) charge and travel towards the negative (–) terminal
Tran will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Av will have a negative (–) charge and travel towards the positive (+) terminal
Tran will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

011e_f3e5

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ferritin (Fer)5.519.8
Hemoglobin (Hem)7.116.7

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both Fer and Hem will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Fer and Hem will have a positive (+) charge and travel towards the negative (–) terminal Correct Fer will have a positive (+) charge and travel towards the negative (–) terminal
Hem will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Fer will have a negative (–) charge and travel towards the positive (+) terminal
Hem will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

7469_1974

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Tropomyosin (Trop)5.135.0
Catalase (Cat)6.765.5

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Trop and Cat will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Trop and Cat will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Trop will have a positive (+) charge and travel towards the negative (–) terminal
Cat will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Trop will have a negative (–) charge and travel towards the positive (+) terminal
Cat will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

c1ae_c994

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Trypsin (Tryp)10.823.5
Casein (Cas)4.924.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Tryp and Cas will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Tryp and Cas will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Tryp will have a positive (+) charge and travel towards the negative (–) terminal
Cas will have a negative (–) charge and travel towards the positive (+) terminal Correct Tryp will have a negative (–) charge and travel towards the positive (+) terminal
Cas will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

e39c_58a5

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Serum Albumin (Alb)4.966.2
Lysozyme (Lys)11.014.4

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Alb and Lys will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Alb and Lys will have a positive (+) charge and travel towards the negative (–) terminal Correct Alb will have a positive (+) charge and travel towards the negative (–) terminal
Lys will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Alb will have a negative (–) charge and travel towards the positive (+) terminal
Lys will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

b7e7_0d4e

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
α-Amylase (Amy)5.961.0
Elastase II (Ela)8.526.5

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Amy and Ela will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Amy and Ela will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Amy will have a positive (+) charge and travel towards the negative (–) terminal
Ela will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Amy will have a negative (–) charge and travel towards the positive (+) terminal
Ela will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

de17_203c

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Urease A (Ure)4.926.5
Chymotrypsin (Chy)8.425.0

Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.5?

Both Ure and Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ure and Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ure will have a positive (+) charge and travel towards the negative (–) terminal
Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ure will have a negative (–) charge and travel towards the positive (+) terminal
Chy will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

e4a2_be8c

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lipase (Lip)4.740.0
G3P Dehydrogenase (GDH)8.336.0

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Lip and GDH will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Lip and GDH will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lip will have a positive (+) charge and travel towards the negative (–) terminal
GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lip will have a negative (–) charge and travel towards the positive (+) terminal
GDH will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

a2fa_992a

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Collagen (Col)6.6134.0
Salmine (Sal)12.13.0

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Col and Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Col and Sal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Col will have a positive (+) charge and travel towards the negative (–) terminal
Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Col will have a negative (–) charge and travel towards the positive (+) terminal
Sal will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

0753_ec0f

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Serine Protease (Ser)4.122.0
Fibrinogen (Fib)5.863.5

Both protein samples are placed into a gel with a constant pH of 3.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.0?

Both Ser and Fib will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ser and Fib will have a positive (+) charge and travel towards the negative (–) terminal Correct Ser will have a positive (+) charge and travel towards the negative (–) terminal
Fib will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ser will have a negative (–) charge and travel towards the positive (+) terminal
Fib will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

992a_fadf

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Epidermal Growth Factor (EGF)4.540.0
Collagen (Col)6.6134.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both EGF and Col will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both EGF and Col will have a positive (+) charge and travel towards the negative (–) terminal Incorrect EGF will have a positive (+) charge and travel towards the negative (–) terminal
Col will have a negative (–) charge and travel towards the positive (+) terminal Incorrect EGF will have a negative (–) charge and travel towards the positive (+) terminal
Col will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

346e_09f1

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Catalase (Cat)6.765.5
Epidermal Growth Factor (EGF)4.540.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Cat and EGF will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Cat and EGF will have a positive (+) charge and travel towards the negative (–) terminal Correct Cat will have a positive (+) charge and travel towards the negative (–) terminal
EGF will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cat will have a negative (–) charge and travel towards the positive (+) terminal
EGF will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

11e3_b28a

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
G3P Dehydrogenase (GDH)8.336.0
Hexokinase (Hex)5.8120.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both GDH and Hex will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both GDH and Hex will have a positive (+) charge and travel towards the negative (–) terminal Incorrect GDH will have a positive (+) charge and travel towards the negative (–) terminal
Hex will have a negative (–) charge and travel towards the positive (+) terminal Correct GDH will have a negative (–) charge and travel towards the positive (+) terminal
Hex will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

6e91_1b5d

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Elastase II (Ela)8.526.5
Ferritin (Fer)5.519.8

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Ela and Fer will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ela and Fer will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ela will have a positive (+) charge and travel towards the negative (–) terminal
Fer will have a negative (–) charge and travel towards the positive (+) terminal Correct Ela will have a negative (–) charge and travel towards the positive (+) terminal
Fer will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

f140_ecd4

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Luciferase (Luc)5.750.0
Elastase II (Ela)8.526.5

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both Luc and Ela will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Luc and Ela will have a positive (+) charge and travel towards the negative (–) terminal Correct Luc will have a positive (+) charge and travel towards the negative (–) terminal
Ela will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Luc will have a negative (–) charge and travel towards the positive (+) terminal
Ela will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

fde1_89f6

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Hexokinase (Hex)5.8120.0
Horseradish peroxidase (HP)9.034.0

Both protein samples are placed into a gel with a constant pH of 10.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.0?

Both Hex and HP will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Hex and HP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Hex will have a positive (+) charge and travel towards the negative (–) terminal
HP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Hex will have a negative (–) charge and travel towards the positive (+) terminal
HP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

999e_9d4e

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Leghemoglobin (Leg)4.716.0
Chymotrypsin (Chy)8.425.0

Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.5?

Both Leg and Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Leg and Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Leg will have a positive (+) charge and travel towards the negative (–) terminal
Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Leg will have a negative (–) charge and travel towards the positive (+) terminal
Chy will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

cef4_42f4

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ovalbumin (Ova)4.645.0
Hemoglobin (Hem)7.116.7

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Ova and Hem will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ova and Hem will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ova will have a positive (+) charge and travel towards the negative (–) terminal
Hem will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ova will have a negative (–) charge and travel towards the positive (+) terminal
Hem will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

c8b1_626c

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Cytochrome c (Cyt)10.213.0
β-Galactosidase (Gal)4.6175.0

Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 11.5?

Both Cyt and Gal will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Cyt and Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Cyt will have a positive (+) charge and travel towards the negative (–) terminal
Gal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cyt will have a negative (–) charge and travel towards the positive (+) terminal
Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

ecf0_a685

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ferritin (Fer)5.519.8
Phospholypase (Pho)10.519.5

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Fer and Pho will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Fer and Pho will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Fer will have a positive (+) charge and travel towards the negative (–) terminal
Pho will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Fer will have a negative (–) charge and travel towards the positive (+) terminal
Pho will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

7a0c_ea9a

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lysozyme (Lys)11.014.4
Chymotrypsin (Chy)8.425.0

Both protein samples are placed into a gel with a constant pH of 12.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 12.0?

Both Lys and Chy will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Lys and Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lys will have a positive (+) charge and travel towards the negative (–) terminal
Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lys will have a negative (–) charge and travel towards the positive (+) terminal
Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

ba22_165c

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Luciferase (Luc)5.750.0
Myelin Basic Protein (MBP)9.518.0

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both Luc and MBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Luc and MBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Luc will have a positive (+) charge and travel towards the negative (–) terminal
MBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Luc will have a negative (–) charge and travel towards the positive (+) terminal
MBP will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

c58b_1556

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Neuraminidase (Neu)5.250.0
Lysozyme (Lys)11.014.4

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Neu and Lys will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Neu and Lys will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Neu will have a positive (+) charge and travel towards the negative (–) terminal
Lys will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Neu will have a negative (–) charge and travel towards the positive (+) terminal
Lys will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

a5d3_7cf3

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Sucrase (Suc)6.551.0
Epidermal Growth Factor (EGF)4.540.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both Suc and EGF will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Suc and EGF will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Suc will have a positive (+) charge and travel towards the negative (–) terminal
EGF will have a negative (–) charge and travel towards the positive (+) terminal Correct Suc will have a negative (–) charge and travel towards the positive (+) terminal
EGF will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

908a_1123

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Salmine (Sal)12.13.0
Phospholypase (Pho)10.519.5

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Sal and Pho will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Sal and Pho will have a positive (+) charge and travel towards the negative (–) terminal Correct Sal will have a positive (+) charge and travel towards the negative (–) terminal
Pho will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Sal will have a negative (–) charge and travel towards the positive (+) terminal
Pho will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

1ae8_610f

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lipase (Lip)4.740.0
Aldolase (Aldo)8.547.5

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Lip and Aldo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lip and Aldo will have a positive (+) charge and travel towards the negative (–) terminal Correct Lip will have a positive (+) charge and travel towards the negative (–) terminal
Aldo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lip will have a negative (–) charge and travel towards the positive (+) terminal
Aldo will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

164f_668c

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Cobra Venom Factor (CVF)5.2149.0
Trypsin (Tryp)10.823.5

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both CVF and Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both CVF and Tryp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect CVF will have a positive (+) charge and travel towards the negative (–) terminal
Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect CVF will have a negative (–) charge and travel towards the positive (+) terminal
Tryp will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

bc0e_6313

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lysozyme (Lys)11.014.4
Tropomyosin (Trop)5.135.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Lys and Trop will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lys and Trop will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lys will have a positive (+) charge and travel towards the negative (–) terminal
Trop will have a negative (–) charge and travel towards the positive (+) terminal Correct Lys will have a negative (–) charge and travel towards the positive (+) terminal
Trop will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

14b8_f3fd

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Insulin (Ins)5.45.8
Ribonuclease A (RibA)9.313.7

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both Ins and RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ins and RibA will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ins will have a positive (+) charge and travel towards the negative (–) terminal
RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ins will have a negative (–) charge and travel towards the positive (+) terminal
RibA will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

281e_c0cb

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Xylosidase (Xyl)5.0100.0
Myoglobin (Myo)7.018.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Xyl and Myo will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Xyl and Myo will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Xyl will have a positive (+) charge and travel towards the negative (–) terminal
Myo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Xyl will have a negative (–) charge and travel towards the positive (+) terminal
Myo will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

65ce_5a54

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Succinate Ligase (SL)6.620.9
Chymotrypsin (Chy)8.425.0

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both SL and Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both SL and Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect SL will have a positive (+) charge and travel towards the negative (–) terminal
Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect SL will have a negative (–) charge and travel towards the positive (+) terminal
Chy will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

a648_dc28

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Pepsin (Pep)1.034.5
Phospholypase (Pho)10.519.5

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Pep and Pho will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Pep and Pho will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Pep will have a positive (+) charge and travel towards the negative (–) terminal
Pho will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Pep will have a negative (–) charge and travel towards the positive (+) terminal
Pho will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

c739_d1d3

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
SARS-CoV-2 Spike Protein (CoV)6.2149.9
Pepsin (Pep)1.034.5

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both CoV and Pep will have a negative (–) charge and travel towards the positive (+) terminal Correct Both CoV and Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect CoV will have a positive (+) charge and travel towards the negative (–) terminal
Pep will have a negative (–) charge and travel towards the positive (+) terminal Incorrect CoV will have a negative (–) charge and travel towards the positive (+) terminal
Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

c60f_8a3a

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Green Fluorescent Protein (GFP)6.228.0
Lipase (Lip)4.740.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both GFP and Lip will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both GFP and Lip will have a positive (+) charge and travel towards the negative (–) terminal Correct GFP will have a positive (+) charge and travel towards the negative (–) terminal
Lip will have a negative (–) charge and travel towards the positive (+) terminal Incorrect GFP will have a negative (–) charge and travel towards the positive (+) terminal
Lip will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

7bf5_2ae1

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Neuraminidase (Neu)5.250.0
Cytochrome c (Cyt)10.213.0

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both Neu and Cyt will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Neu and Cyt will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Neu will have a positive (+) charge and travel towards the negative (–) terminal
Cyt will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Neu will have a negative (–) charge and travel towards the positive (+) terminal
Cyt will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

986d_b285

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ovalbumin (Ova)4.645.0
Collagen (Col)6.6134.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both Ova and Col will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ova and Col will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ova will have a positive (+) charge and travel towards the negative (–) terminal
Col will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ova will have a negative (–) charge and travel towards the positive (+) terminal
Col will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

0ee5_38bd

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Neuraminidase (Neu)5.250.0
Chymotrypsin (Chy)8.425.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Neu and Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Neu and Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Neu will have a positive (+) charge and travel towards the negative (–) terminal
Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Neu will have a negative (–) charge and travel towards the positive (+) terminal
Chy will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

44ce_7339

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Pepsin (Pep)1.034.5
Heat Shock Protein (HSP)5.570.1

Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.5?

Both Pep and HSP will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Pep and HSP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Pep will have a positive (+) charge and travel towards the negative (–) terminal
HSP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Pep will have a negative (–) charge and travel towards the positive (+) terminal
HSP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

8691_44df

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Immunoglobulin (IgG)7.3145.0
Ribonuclease A (RibA)9.313.7

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both IgG and RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both IgG and RibA will have a positive (+) charge and travel towards the negative (–) terminal Correct IgG will have a positive (+) charge and travel towards the negative (–) terminal
RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect IgG will have a negative (–) charge and travel towards the positive (+) terminal
RibA will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

be55_3781

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Succinate Ligase (SL)6.620.9
Epidermal Growth Factor (EGF)4.540.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both SL and EGF will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both SL and EGF will have a positive (+) charge and travel towards the negative (–) terminal Correct SL will have a positive (+) charge and travel towards the negative (–) terminal
EGF will have a negative (–) charge and travel towards the positive (+) terminal Incorrect SL will have a negative (–) charge and travel towards the positive (+) terminal
EGF will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

468f_65b6

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ovalbumin (Ova)4.645.0
Sucrase (Suc)6.551.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Ova and Suc will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ova and Suc will have a positive (+) charge and travel towards the negative (–) terminal Correct Ova will have a positive (+) charge and travel towards the negative (–) terminal
Suc will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ova will have a negative (–) charge and travel towards the positive (+) terminal
Suc will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

182e_c0c4

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Insulin (Ins)5.45.8
Aprotinin (Apr)9.26.5

Both protein samples are placed into a gel with a constant pH of 4.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.0?

Both Ins and Apr will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ins and Apr will have a positive (+) charge and travel towards the negative (–) terminal Correct Ins will have a positive (+) charge and travel towards the negative (–) terminal
Apr will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ins will have a negative (–) charge and travel towards the positive (+) terminal
Apr will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

2f6d_01d8

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Aprotinin (Apr)9.26.5
Streptavidin (Stp)6.013.2

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both Apr and Stp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Apr and Stp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Apr will have a positive (+) charge and travel towards the negative (–) terminal
Stp will have a negative (–) charge and travel towards the positive (+) terminal Correct Apr will have a negative (–) charge and travel towards the positive (+) terminal
Stp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

6e02_9bee

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Trypsin (Tryp)10.823.5
Streptavidin (Stp)6.013.2

Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.5?

Both Tryp and Stp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Tryp and Stp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Tryp will have a positive (+) charge and travel towards the negative (–) terminal
Stp will have a negative (–) charge and travel towards the positive (+) terminal Correct Tryp will have a negative (–) charge and travel towards the positive (+) terminal
Stp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

886d_3ef0

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Cytochrome c (Cyt)10.213.0
Chymotrypsin (Chy)8.425.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Cyt and Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Cyt and Chy will have a positive (+) charge and travel towards the negative (–) terminal Correct Cyt will have a positive (+) charge and travel towards the negative (–) terminal
Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cyt will have a negative (–) charge and travel towards the positive (+) terminal
Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

2c36_258e

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Serum Albumin (Alb)4.966.2
Pepsin (Pep)1.034.5

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Alb and Pep will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Alb and Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Alb will have a positive (+) charge and travel towards the negative (–) terminal
Pep will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Alb will have a negative (–) charge and travel towards the positive (+) terminal
Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

2cf8_761d

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Sucrase (Suc)6.551.0
Ovalbumin (Ova)4.645.0

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both Suc and Ova will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Suc and Ova will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Suc will have a positive (+) charge and travel towards the negative (–) terminal
Ova will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Suc will have a negative (–) charge and travel towards the positive (+) terminal
Ova will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

9100_a99b

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Cytochrome c (Cyt)10.213.0
SARS-CoV-2 Spike Protein (CoV)6.2149.9

Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 11.5?

Both Cyt and CoV will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Cyt and CoV will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Cyt will have a positive (+) charge and travel towards the negative (–) terminal
CoV will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cyt will have a negative (–) charge and travel towards the positive (+) terminal
CoV will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

7771_6888

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Hexokinase (Hex)5.8120.0
Avidin (Av)10.516.9

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Hex and Av will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Hex and Av will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Hex will have a positive (+) charge and travel towards the negative (–) terminal
Av will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Hex will have a negative (–) charge and travel towards the positive (+) terminal
Av will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

25cb_78cb

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ferritin (Fer)5.519.8
Myoglobin (Myo)7.018.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Fer and Myo will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Fer and Myo will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Fer will have a positive (+) charge and travel towards the negative (–) terminal
Myo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Fer will have a negative (–) charge and travel towards the positive (+) terminal
Myo will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

9521_c7c0

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Pepsin (Pep)1.034.5
Fumerase (Fum)7.648.5

Both protein samples are placed into a gel with a constant pH of 9.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.0?

Both Pep and Fum will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Pep and Fum will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Pep will have a positive (+) charge and travel towards the negative (–) terminal
Fum will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Pep will have a negative (–) charge and travel towards the positive (+) terminal
Fum will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

868a_0cbc

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Succinate Ligase (SL)6.620.9
Lipase (Lip)4.740.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both SL and Lip will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both SL and Lip will have a positive (+) charge and travel towards the negative (–) terminal Incorrect SL will have a positive (+) charge and travel towards the negative (–) terminal
Lip will have a negative (–) charge and travel towards the positive (+) terminal Correct SL will have a negative (–) charge and travel towards the positive (+) terminal
Lip will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

7694_44df

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
γ-Globulin (Glob)6.657.0
Ribonuclease A (RibA)9.313.7

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Glob and RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Glob and RibA will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Glob will have a positive (+) charge and travel towards the negative (–) terminal
RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Glob will have a negative (–) charge and travel towards the positive (+) terminal
RibA will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

be1c_60df

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Neuraminidase (Neu)5.250.0
Phospholypase (Pho)10.519.5

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Neu and Pho will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Neu and Pho will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Neu will have a positive (+) charge and travel towards the negative (–) terminal
Pho will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Neu will have a negative (–) charge and travel towards the positive (+) terminal
Pho will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

696e_922b

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Serine Protease (Ser)4.122.0
Cytochrome c (Cyt)10.213.0

Both protein samples are placed into a gel with a constant pH of 3.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.0?

Both Ser and Cyt will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ser and Cyt will have a positive (+) charge and travel towards the negative (–) terminal Correct Ser will have a positive (+) charge and travel towards the negative (–) terminal
Cyt will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ser will have a negative (–) charge and travel towards the positive (+) terminal
Cyt will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

20ce_93ce

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Green Fluorescent Protein (GFP)6.228.0
Ribonuclease A (RibA)9.313.7

Both protein samples are placed into a gel with a constant pH of 5.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.0?

Both GFP and RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both GFP and RibA will have a positive (+) charge and travel towards the negative (–) terminal Correct GFP will have a positive (+) charge and travel towards the negative (–) terminal
RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect GFP will have a negative (–) charge and travel towards the positive (+) terminal
RibA will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

bf44_9d4e

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Leghemoglobin (Leg)4.716.0
Chymotrypsin (Chy)8.425.0

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Leg and Chy will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Leg and Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Leg will have a positive (+) charge and travel towards the negative (–) terminal
Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Leg will have a negative (–) charge and travel towards the positive (+) terminal
Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

ae03_14d3

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Chymotrypsin (Chy)8.425.0
β-Galactosidase (Gal)4.6175.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Chy and Gal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Chy and Gal will have a positive (+) charge and travel towards the negative (–) terminal Correct Chy will have a positive (+) charge and travel towards the negative (–) terminal
Gal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Chy will have a negative (–) charge and travel towards the positive (+) terminal
Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

10be_4d3e

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Collagen (Col)6.6134.0
Epidermal Growth Factor (EGF)4.540.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both Col and EGF will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Col and EGF will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Col will have a positive (+) charge and travel towards the negative (–) terminal
EGF will have a negative (–) charge and travel towards the positive (+) terminal Correct Col will have a negative (–) charge and travel towards the positive (+) terminal
EGF will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

2958_87dc

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Pepsin (Pep)1.034.5
G3P Dehydrogenase (GDH)8.336.0

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both Pep and GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Pep and GDH will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Pep will have a positive (+) charge and travel towards the negative (–) terminal
GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Pep will have a negative (–) charge and travel towards the positive (+) terminal
GDH will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

f504_fd71

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ferritin (Fer)5.519.8
G3P Dehydrogenase (GDH)8.336.0

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Fer and GDH will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Fer and GDH will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Fer will have a positive (+) charge and travel towards the negative (–) terminal
GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Fer will have a negative (–) charge and travel towards the positive (+) terminal
GDH will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

d8b1_8285

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
SARS-CoV-2 Spike Protein (CoV)6.2149.9
G3P Dehydrogenase (GDH)8.336.0

Both protein samples are placed into a gel with a constant pH of 5.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.0?

Both CoV and GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both CoV and GDH will have a positive (+) charge and travel towards the negative (–) terminal Correct CoV will have a positive (+) charge and travel towards the negative (–) terminal
GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect CoV will have a negative (–) charge and travel towards the positive (+) terminal
GDH will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

ecab_94ed

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Salmine (Sal)12.13.0
γ-Globulin (Glob)6.657.0

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Sal and Glob will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Sal and Glob will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Sal will have a positive (+) charge and travel towards the negative (–) terminal
Glob will have a negative (–) charge and travel towards the positive (+) terminal Correct Sal will have a negative (–) charge and travel towards the positive (+) terminal
Glob will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

3879_b7bf

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ribonuclease A (RibA)9.313.7
Heat Shock Protein (HSP)5.570.1

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both RibA and HSP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both RibA and HSP will have a positive (+) charge and travel towards the negative (–) terminal Correct RibA will have a positive (+) charge and travel towards the negative (–) terminal
HSP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect RibA will have a negative (–) charge and travel towards the positive (+) terminal
HSP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

037d_c5c2

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
γ-Globulin (Glob)6.657.0
Horseradish peroxidase (HP)9.034.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both Glob and HP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Glob and HP will have a positive (+) charge and travel towards the negative (–) terminal Correct Glob will have a positive (+) charge and travel towards the negative (–) terminal
HP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Glob will have a negative (–) charge and travel towards the positive (+) terminal
HP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

a6c7_1047

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Elastase II (Ela)8.526.5
Serum Albumin (Alb)4.966.2

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Ela and Alb will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ela and Alb will have a positive (+) charge and travel towards the negative (–) terminal Correct Ela will have a positive (+) charge and travel towards the negative (–) terminal
Alb will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ela will have a negative (–) charge and travel towards the positive (+) terminal
Alb will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

41ef_a8c6

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ribonuclease A (RibA)9.313.7
Tropomyosin (Trop)5.135.0

Both protein samples are placed into a gel with a constant pH of 10.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.5?

Both RibA and Trop will have a negative (–) charge and travel towards the positive (+) terminal Correct Both RibA and Trop will have a positive (+) charge and travel towards the negative (–) terminal Incorrect RibA will have a positive (+) charge and travel towards the negative (–) terminal
Trop will have a negative (–) charge and travel towards the positive (+) terminal Incorrect RibA will have a negative (–) charge and travel towards the positive (+) terminal
Trop will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

e8c3_ed5b

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Insulin (Ins)5.45.8
Myelin Basic Protein (MBP)9.518.0

Both protein samples are placed into a gel with a constant pH of 4.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.0?

Both Ins and MBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ins and MBP will have a positive (+) charge and travel towards the negative (–) terminal Correct Ins will have a positive (+) charge and travel towards the negative (–) terminal
MBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ins will have a negative (–) charge and travel towards the positive (+) terminal
MBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

84ab_fed2

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ferritin (Fer)5.519.8
Salmine (Sal)12.13.0

Both protein samples are placed into a gel with a constant pH of 9.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.0?

Both Fer and Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Fer and Sal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Fer will have a positive (+) charge and travel towards the negative (–) terminal
Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Fer will have a negative (–) charge and travel towards the positive (+) terminal
Sal will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

273d_0901

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Leghemoglobin (Leg)4.716.0
γ-Globulin (Glob)6.657.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both Leg and Glob will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Leg and Glob will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Leg will have a positive (+) charge and travel towards the negative (–) terminal
Glob will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Leg will have a negative (–) charge and travel towards the positive (+) terminal
Glob will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

fd8f_cb6f

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Cytochrome c (Cyt)10.213.0
Urease A (Ure)4.926.5

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both Cyt and Ure will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Cyt and Ure will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Cyt will have a positive (+) charge and travel towards the negative (–) terminal
Ure will have a negative (–) charge and travel towards the positive (+) terminal Correct Cyt will have a negative (–) charge and travel towards the positive (+) terminal
Ure will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

d437_e458

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Agglutinin (Agg)4.822.0
Collagen (Col)6.6134.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both Agg and Col will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Agg and Col will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Agg will have a positive (+) charge and travel towards the negative (–) terminal
Col will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Agg will have a negative (–) charge and travel towards the positive (+) terminal
Col will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

c48b_82cb

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
β-Galactosidase (Gal)4.6175.0
Immunoglobulin (IgG)7.3145.0

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Gal and IgG will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Gal and IgG will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Gal will have a positive (+) charge and travel towards the negative (–) terminal
IgG will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Gal will have a negative (–) charge and travel towards the positive (+) terminal
IgG will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

6be0_0d53

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Heat Shock Protein (HSP)5.570.1
G3P Dehydrogenase (GDH)8.336.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both HSP and GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both HSP and GDH will have a positive (+) charge and travel towards the negative (–) terminal Incorrect HSP will have a positive (+) charge and travel towards the negative (–) terminal
GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect HSP will have a negative (–) charge and travel towards the positive (+) terminal
GDH will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

c270_097f

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Chymotrypsin (Chy)8.425.0
Transferrin (Tran)5.980.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Chy and Tran will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Chy and Tran will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Chy will have a positive (+) charge and travel towards the negative (–) terminal
Tran will have a negative (–) charge and travel towards the positive (+) terminal Correct Chy will have a negative (–) charge and travel towards the positive (+) terminal
Tran will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

c4dc_9c23

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Catalase (Cat)6.765.5
Myelin Basic Protein (MBP)9.518.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Cat and MBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Cat and MBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Cat will have a positive (+) charge and travel towards the negative (–) terminal
MBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cat will have a negative (–) charge and travel towards the positive (+) terminal
MBP will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

5c26_d757

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
G3P Dehydrogenase (GDH)8.336.0
Neuraminidase (Neu)5.250.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both GDH and Neu will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both GDH and Neu will have a positive (+) charge and travel towards the negative (–) terminal Incorrect GDH will have a positive (+) charge and travel towards the negative (–) terminal
Neu will have a negative (–) charge and travel towards the positive (+) terminal Correct GDH will have a negative (–) charge and travel towards the positive (+) terminal
Neu will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

4a0c_b5a3

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
α-Amylase (Amy)5.961.0
G3P Dehydrogenase (GDH)8.336.0

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both Amy and GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Amy and GDH will have a positive (+) charge and travel towards the negative (–) terminal Correct Amy will have a positive (+) charge and travel towards the negative (–) terminal
GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Amy will have a negative (–) charge and travel towards the positive (+) terminal
GDH will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

6306_4fc3

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ovalbumin (Ova)4.645.0
Salmine (Sal)12.13.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Ova and Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ova and Sal will have a positive (+) charge and travel towards the negative (–) terminal Correct Ova will have a positive (+) charge and travel towards the negative (–) terminal
Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ova will have a negative (–) charge and travel towards the positive (+) terminal
Sal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

7c2c_2ca3

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Myelin Basic Protein (MBP)9.518.0
β-Galactosidase (Gal)4.6175.0

Both protein samples are placed into a gel with a constant pH of 10.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.5?

Both MBP and Gal will have a negative (–) charge and travel towards the positive (+) terminal Correct Both MBP and Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MBP will have a positive (+) charge and travel towards the negative (–) terminal
Gal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect MBP will have a negative (–) charge and travel towards the positive (+) terminal
Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

0d1c_833f

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ribonuclease A (RibA)9.313.7
Histone (His)10.815.0

Both protein samples are placed into a gel with a constant pH of 10.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.0?

Both RibA and His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both RibA and His will have a positive (+) charge and travel towards the negative (–) terminal Incorrect RibA will have a positive (+) charge and travel towards the negative (–) terminal
His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect RibA will have a negative (–) charge and travel towards the positive (+) terminal
His will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

7a4e_88c8

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Aprotinin (Apr)9.26.5
Collagen (Col)6.6134.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both Apr and Col will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Apr and Col will have a positive (+) charge and travel towards the negative (–) terminal Correct Apr will have a positive (+) charge and travel towards the negative (–) terminal
Col will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Apr will have a negative (–) charge and travel towards the positive (+) terminal
Col will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

c1c7_257b

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Aprotinin (Apr)9.26.5
Pepsin (Pep)1.034.5

Both protein samples are placed into a gel with a constant pH of 5.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.0?

Both Apr and Pep will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Apr and Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Apr will have a positive (+) charge and travel towards the negative (–) terminal
Pep will have a negative (–) charge and travel towards the positive (+) terminal Correct Apr will have a negative (–) charge and travel towards the positive (+) terminal
Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

483b_1de9

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lysozyme (Lys)11.014.4
Heat Shock Protein (HSP)5.570.1

Both protein samples are placed into a gel with a constant pH of 12.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 12.0?

Both Lys and HSP will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Lys and HSP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lys will have a positive (+) charge and travel towards the negative (–) terminal
HSP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lys will have a negative (–) charge and travel towards the positive (+) terminal
HSP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

db2f_6b30

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Horseradish peroxidase (HP)9.034.0
Agglutinin (Agg)4.822.0

Both protein samples are placed into a gel with a constant pH of 10.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.0?

Both HP and Agg will have a negative (–) charge and travel towards the positive (+) terminal Correct Both HP and Agg will have a positive (+) charge and travel towards the negative (–) terminal Incorrect HP will have a positive (+) charge and travel towards the negative (–) terminal
Agg will have a negative (–) charge and travel towards the positive (+) terminal Incorrect HP will have a negative (–) charge and travel towards the positive (+) terminal
Agg will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

2af3_90cd

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Fumerase (Fum)7.648.5
Aprotinin (Apr)9.26.5

Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.5?

Both Fum and Apr will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Fum and Apr will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Fum will have a positive (+) charge and travel towards the negative (–) terminal
Apr will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Fum will have a negative (–) charge and travel towards the positive (+) terminal
Apr will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

2ac5_f03b

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Catalase (Cat)6.765.5
Lysozyme (Lys)11.014.4

Both protein samples are placed into a gel with a constant pH of 12.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 12.0?

Both Cat and Lys will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Cat and Lys will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Cat will have a positive (+) charge and travel towards the negative (–) terminal
Lys will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cat will have a negative (–) charge and travel towards the positive (+) terminal
Lys will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

b215_ad3f

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Serum Albumin (Alb)4.966.2
Immunoglobulin (IgG)7.3145.0

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Alb and IgG will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Alb and IgG will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Alb will have a positive (+) charge and travel towards the negative (–) terminal
IgG will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Alb will have a negative (–) charge and travel towards the positive (+) terminal
IgG will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

8565_4a05

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
SARS-CoV-2 Spike Protein (CoV)6.2149.9
Avidin (Av)10.516.9

Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 11.5?

Both CoV and Av will have a negative (–) charge and travel towards the positive (+) terminal Correct Both CoV and Av will have a positive (+) charge and travel towards the negative (–) terminal Incorrect CoV will have a positive (+) charge and travel towards the negative (–) terminal
Av will have a negative (–) charge and travel towards the positive (+) terminal Incorrect CoV will have a negative (–) charge and travel towards the positive (+) terminal
Av will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

5395_8342

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Pyruvate Kinase (PK)5.660.0
Salmine (Sal)12.13.0

Both protein samples are placed into a gel with a constant pH of 9.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.0?

Both PK and Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both PK and Sal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect PK will have a positive (+) charge and travel towards the negative (–) terminal
Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect PK will have a negative (–) charge and travel towards the positive (+) terminal
Sal will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

4023_ae18

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Insulin (Ins)5.45.8
Aldolase (Aldo)8.547.5

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Ins and Aldo will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Ins and Aldo will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ins will have a positive (+) charge and travel towards the negative (–) terminal
Aldo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ins will have a negative (–) charge and travel towards the positive (+) terminal
Aldo will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

f50c_6394

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Estrogen Binding Protein (EBP)4.9115.0
Avidin (Av)10.516.9

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both EBP and Av will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both EBP and Av will have a positive (+) charge and travel towards the negative (–) terminal Correct EBP will have a positive (+) charge and travel towards the negative (–) terminal
Av will have a negative (–) charge and travel towards the positive (+) terminal Incorrect EBP will have a negative (–) charge and travel towards the positive (+) terminal
Av will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

bd5c_b108

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Succinate Ligase (SL)6.620.9
Estrogen Binding Protein (EBP)4.9115.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both SL and EBP will have a negative (–) charge and travel towards the positive (+) terminal Correct Both SL and EBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect SL will have a positive (+) charge and travel towards the negative (–) terminal
EBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect SL will have a negative (–) charge and travel towards the positive (+) terminal
EBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

975f_2d3e

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Urease A (Ure)4.926.5
Hemoglobin (Hem)7.116.7

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Ure and Hem will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ure and Hem will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ure will have a positive (+) charge and travel towards the negative (–) terminal
Hem will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ure will have a negative (–) charge and travel towards the positive (+) terminal
Hem will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

d4a0_ae81

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Transferrin (Tran)5.980.0
Elastase II (Ela)8.526.5

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Tran and Ela will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Tran and Ela will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Tran will have a positive (+) charge and travel towards the negative (–) terminal
Ela will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Tran will have a negative (–) charge and travel towards the positive (+) terminal
Ela will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

2fa6_3199

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Hemoglobin (Hem)7.116.7
Lipase (Lip)4.740.0

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Hem and Lip will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Hem and Lip will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Hem will have a positive (+) charge and travel towards the negative (–) terminal
Lip will have a negative (–) charge and travel towards the positive (+) terminal Correct Hem will have a negative (–) charge and travel towards the positive (+) terminal
Lip will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

b29b_9ba0

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Chymotrypsin (Chy)8.425.0
Leghemoglobin (Leg)4.716.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Chy and Leg will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Chy and Leg will have a positive (+) charge and travel towards the negative (–) terminal Correct Chy will have a positive (+) charge and travel towards the negative (–) terminal
Leg will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Chy will have a negative (–) charge and travel towards the positive (+) terminal
Leg will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

0636_a722

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Immunoglobulin (IgG)7.3145.0
Aldehyde Dehydrogenase (Alde)5.353.0

Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.5?

Both IgG and Alde will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both IgG and Alde will have a positive (+) charge and travel towards the negative (–) terminal Incorrect IgG will have a positive (+) charge and travel towards the negative (–) terminal
Alde will have a negative (–) charge and travel towards the positive (+) terminal Correct IgG will have a negative (–) charge and travel towards the positive (+) terminal
Alde will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

e4ee_5ac1

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Urease A (Ure)4.926.5
Succinate Ligase (SL)6.620.9

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Ure and SL will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Ure and SL will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ure will have a positive (+) charge and travel towards the negative (–) terminal
SL will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ure will have a negative (–) charge and travel towards the positive (+) terminal
SL will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

10ea_9b47

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Urease A (Ure)4.926.5
Elastase II (Ela)8.526.5

Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.5?

Both Ure and Ela will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ure and Ela will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ure will have a positive (+) charge and travel towards the negative (–) terminal
Ela will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ure will have a negative (–) charge and travel towards the positive (+) terminal
Ela will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

07fb_b048

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ovalbumin (Ova)4.645.0
Horseradish peroxidase (HP)9.034.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Ova and HP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ova and HP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ova will have a positive (+) charge and travel towards the negative (–) terminal
HP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ova will have a negative (–) charge and travel towards the positive (+) terminal
HP will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

e122_68c6

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Histone (His)10.815.0
Agglutinin (Agg)4.822.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both His and Agg will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both His and Agg will have a positive (+) charge and travel towards the negative (–) terminal Incorrect His will have a positive (+) charge and travel towards the negative (–) terminal
Agg will have a negative (–) charge and travel towards the positive (+) terminal Correct His will have a negative (–) charge and travel towards the positive (+) terminal
Agg will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

530e_9e2b

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Cobra Venom Factor (CVF)5.2149.0
Pepsin (Pep)1.034.5

Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.5?

Both CVF and Pep will have a negative (–) charge and travel towards the positive (+) terminal Correct Both CVF and Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect CVF will have a positive (+) charge and travel towards the negative (–) terminal
Pep will have a negative (–) charge and travel towards the positive (+) terminal Incorrect CVF will have a negative (–) charge and travel towards the positive (+) terminal
Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

390a_4262

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Hexokinase (Hex)5.8120.0
Histone (His)10.815.0

Both protein samples are placed into a gel with a constant pH of 12.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 12.0?

Both Hex and His will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Hex and His will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Hex will have a positive (+) charge and travel towards the negative (–) terminal
His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Hex will have a negative (–) charge and travel towards the positive (+) terminal
His will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

c515_7b67

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Horseradish peroxidase (HP)9.034.0
Salmine (Sal)12.13.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both HP and Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both HP and Sal will have a positive (+) charge and travel towards the negative (–) terminal Correct HP will have a positive (+) charge and travel towards the negative (–) terminal
Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect HP will have a negative (–) charge and travel towards the positive (+) terminal
Sal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

acc2_2437

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Chymotrypsin (Chy)8.425.0
Fibrinogen (Fib)5.863.5

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Chy and Fib will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Chy and Fib will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Chy will have a positive (+) charge and travel towards the negative (–) terminal
Fib will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Chy will have a negative (–) charge and travel towards the positive (+) terminal
Fib will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

9489_be91

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Neuraminidase (Neu)5.250.0
Myoglobin (Myo)7.018.0

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Neu and Myo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Neu and Myo will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Neu will have a positive (+) charge and travel towards the negative (–) terminal
Myo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Neu will have a negative (–) charge and travel towards the positive (+) terminal
Myo will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

9129_8a88

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Immunoglobulin (IgG)7.3145.0
Myelin Basic Protein (MBP)9.518.0

Both protein samples are placed into a gel with a constant pH of 10.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.5?

Both IgG and MBP will have a negative (–) charge and travel towards the positive (+) terminal Correct Both IgG and MBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect IgG will have a positive (+) charge and travel towards the negative (–) terminal
MBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect IgG will have a negative (–) charge and travel towards the positive (+) terminal
MBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

c3dd_920c

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Immunoglobulin (IgG)7.3145.0
Hexokinase (Hex)5.8120.0

Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.5?

Both IgG and Hex will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both IgG and Hex will have a positive (+) charge and travel towards the negative (–) terminal Incorrect IgG will have a positive (+) charge and travel towards the negative (–) terminal
Hex will have a negative (–) charge and travel towards the positive (+) terminal Correct IgG will have a negative (–) charge and travel towards the positive (+) terminal
Hex will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

8e36_efb1

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Horseradish peroxidase (HP)9.034.0
Ovalbumin (Ova)4.645.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both HP and Ova will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both HP and Ova will have a positive (+) charge and travel towards the negative (–) terminal Incorrect HP will have a positive (+) charge and travel towards the negative (–) terminal
Ova will have a negative (–) charge and travel towards the positive (+) terminal Correct HP will have a negative (–) charge and travel towards the positive (+) terminal
Ova will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

40b0_b3ed

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lysozyme (Lys)11.014.4
Hexokinase (Hex)5.8120.0

Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.5?

Both Lys and Hex will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lys and Hex will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lys will have a positive (+) charge and travel towards the negative (–) terminal
Hex will have a negative (–) charge and travel towards the positive (+) terminal Correct Lys will have a negative (–) charge and travel towards the positive (+) terminal
Hex will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

ae08_9bde

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
α-Amylase (Amy)5.961.0
Lysozyme (Lys)11.014.4

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both Amy and Lys will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Amy and Lys will have a positive (+) charge and travel towards the negative (–) terminal Correct Amy will have a positive (+) charge and travel towards the negative (–) terminal
Lys will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Amy will have a negative (–) charge and travel towards the positive (+) terminal
Lys will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

e313_b17f

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Aldolase (Aldo)8.547.5
Ovalbumin (Ova)4.645.0

Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.5?

Both Aldo and Ova will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Aldo and Ova will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Aldo will have a positive (+) charge and travel towards the negative (–) terminal
Ova will have a negative (–) charge and travel towards the positive (+) terminal Correct Aldo will have a negative (–) charge and travel towards the positive (+) terminal
Ova will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

b5ca_b2c8

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
γ-Globulin (Glob)6.657.0
Chymotrypsin (Chy)8.425.0

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Glob and Chy will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Glob and Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Glob will have a positive (+) charge and travel towards the negative (–) terminal
Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Glob will have a negative (–) charge and travel towards the positive (+) terminal
Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

72af_eae1

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Salmine (Sal)12.13.0
Cobra Venom Factor (CVF)5.2149.0

Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.5?

Both Sal and CVF will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Sal and CVF will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Sal will have a positive (+) charge and travel towards the negative (–) terminal
CVF will have a negative (–) charge and travel towards the positive (+) terminal Correct Sal will have a negative (–) charge and travel towards the positive (+) terminal
CVF will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

4623_92cc

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Horseradish peroxidase (HP)9.034.0
Succinate Ligase (SL)6.620.9

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both HP and SL will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both HP and SL will have a positive (+) charge and travel towards the negative (–) terminal Correct HP will have a positive (+) charge and travel towards the negative (–) terminal
SL will have a negative (–) charge and travel towards the positive (+) terminal Incorrect HP will have a negative (–) charge and travel towards the positive (+) terminal
SL will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

37e6_2254

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Horseradish peroxidase (HP)9.034.0
Heat Shock Protein (HSP)5.570.1

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both HP and HSP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both HP and HSP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect HP will have a positive (+) charge and travel towards the negative (–) terminal
HSP will have a negative (–) charge and travel towards the positive (+) terminal Correct HP will have a negative (–) charge and travel towards the positive (+) terminal
HSP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

9623_63b6

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Urease A (Ure)4.926.5
Fumerase (Fum)7.648.5

Both protein samples are placed into a gel with a constant pH of 9.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.0?

Both Ure and Fum will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Ure and Fum will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ure will have a positive (+) charge and travel towards the negative (–) terminal
Fum will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ure will have a negative (–) charge and travel towards the positive (+) terminal
Fum will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

49dd_9ace

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Elastase II (Ela)8.526.5
Actin (Act)5.343.0

Both protein samples are placed into a gel with a constant pH of 4.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.0?

Both Ela and Act will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ela and Act will have a positive (+) charge and travel towards the negative (–) terminal Correct Ela will have a positive (+) charge and travel towards the negative (–) terminal
Act will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ela will have a negative (–) charge and travel towards the positive (+) terminal
Act will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

1e27_56b6

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Agglutinin (Agg)4.822.0
Avidin (Av)10.516.9

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both Agg and Av will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Agg and Av will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Agg will have a positive (+) charge and travel towards the negative (–) terminal
Av will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Agg will have a negative (–) charge and travel towards the positive (+) terminal
Av will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

9569_acbf

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Transferrin (Tran)5.980.0
Avidin (Av)10.516.9

Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 11.5?

Both Tran and Av will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Tran and Av will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Tran will have a positive (+) charge and travel towards the negative (–) terminal
Av will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Tran will have a negative (–) charge and travel towards the positive (+) terminal
Av will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

204a_66ae

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ribonuclease A (RibA)9.313.7
Serine Protease (Ser)4.122.0

Both protein samples are placed into a gel with a constant pH of 10.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.5?

Both RibA and Ser will have a negative (–) charge and travel towards the positive (+) terminal Correct Both RibA and Ser will have a positive (+) charge and travel towards the negative (–) terminal Incorrect RibA will have a positive (+) charge and travel towards the negative (–) terminal
Ser will have a negative (–) charge and travel towards the positive (+) terminal Incorrect RibA will have a negative (–) charge and travel towards the positive (+) terminal
Ser will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

8efb_86f5

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Fibrinogen (Fib)5.863.5
Histone (His)10.815.0

Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.5?

Both Fib and His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Fib and His will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Fib will have a positive (+) charge and travel towards the negative (–) terminal
His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Fib will have a negative (–) charge and travel towards the positive (+) terminal
His will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

e07e_46ee

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Pyruvate Kinase (PK)5.660.0
Pepsin (Pep)1.034.5

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both PK and Pep will have a negative (–) charge and travel towards the positive (+) terminal Correct Both PK and Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect PK will have a positive (+) charge and travel towards the negative (–) terminal
Pep will have a negative (–) charge and travel towards the positive (+) terminal Incorrect PK will have a negative (–) charge and travel towards the positive (+) terminal
Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

87c4_819b

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Leghemoglobin (Leg)4.716.0
Catalase (Cat)6.765.5

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both Leg and Cat will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Leg and Cat will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Leg will have a positive (+) charge and travel towards the negative (–) terminal
Cat will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Leg will have a negative (–) charge and travel towards the positive (+) terminal
Cat will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

df02_d0ac

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ovalbumin (Ova)4.645.0
Ribonuclease A (RibA)9.313.7

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Ova and RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ova and RibA will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ova will have a positive (+) charge and travel towards the negative (–) terminal
RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ova will have a negative (–) charge and travel towards the positive (+) terminal
RibA will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

38cd_2275

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Prostate-Specific Antigen (PSA)6.430.0
Lipase (Lip)4.740.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both PSA and Lip will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both PSA and Lip will have a positive (+) charge and travel towards the negative (–) terminal Incorrect PSA will have a positive (+) charge and travel towards the negative (–) terminal
Lip will have a negative (–) charge and travel towards the positive (+) terminal Correct PSA will have a negative (–) charge and travel towards the positive (+) terminal
Lip will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

3ac3_c383

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Cytochrome c (Cyt)10.213.0
Collagen (Col)6.6134.0

Both protein samples are placed into a gel with a constant pH of 11.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 11.5?

Both Cyt and Col will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Cyt and Col will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Cyt will have a positive (+) charge and travel towards the negative (–) terminal
Col will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cyt will have a negative (–) charge and travel towards the positive (+) terminal
Col will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

217a_4707

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
α-Amylase (Amy)5.961.0
Horseradish peroxidase (HP)9.034.0

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both Amy and HP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Amy and HP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Amy will have a positive (+) charge and travel towards the negative (–) terminal
HP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Amy will have a negative (–) charge and travel towards the positive (+) terminal
HP will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

7036_f48d

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ovalbumin (Ova)4.645.0
Elastase II (Ela)8.526.5

Both protein samples are placed into a gel with a constant pH of 6.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.5?

Both Ova and Ela will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ova and Ela will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ova will have a positive (+) charge and travel towards the negative (–) terminal
Ela will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ova will have a negative (–) charge and travel towards the positive (+) terminal
Ela will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

b817_783d

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lipase (Lip)4.740.0
Histone (His)10.815.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Lip and His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lip and His will have a positive (+) charge and travel towards the negative (–) terminal Correct Lip will have a positive (+) charge and travel towards the negative (–) terminal
His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lip will have a negative (–) charge and travel towards the positive (+) terminal
His will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

ed1a_6827

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Myelin Basic Protein (MBP)9.518.0
Xylosidase (Xyl)5.0100.0

Both protein samples are placed into a gel with a constant pH of 10.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.5?

Both MBP and Xyl will have a negative (–) charge and travel towards the positive (+) terminal Correct Both MBP and Xyl will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MBP will have a positive (+) charge and travel towards the negative (–) terminal
Xyl will have a negative (–) charge and travel towards the positive (+) terminal Incorrect MBP will have a negative (–) charge and travel towards the positive (+) terminal
Xyl will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

d9f3_1de9

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lysozyme (Lys)11.014.4
Heat Shock Protein (HSP)5.570.1

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Lys and HSP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lys and HSP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lys will have a positive (+) charge and travel towards the negative (–) terminal
HSP will have a negative (–) charge and travel towards the positive (+) terminal Correct Lys will have a negative (–) charge and travel towards the positive (+) terminal
HSP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

45c6_18ce

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Aldolase (Aldo)8.547.5
Ferritin (Fer)5.519.8

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Aldo and Fer will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Aldo and Fer will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Aldo will have a positive (+) charge and travel towards the negative (–) terminal
Fer will have a negative (–) charge and travel towards the positive (+) terminal Correct Aldo will have a negative (–) charge and travel towards the positive (+) terminal
Fer will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

48b7_4084

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Casein (Cas)4.924.0
Salmine (Sal)12.13.0

Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.5?

Both Cas and Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Cas and Sal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Cas will have a positive (+) charge and travel towards the negative (–) terminal
Sal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cas will have a negative (–) charge and travel towards the positive (+) terminal
Sal will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

1036_85eb

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Streptavidin (Stp)6.013.2
Chymotrypsin (Chy)8.425.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Stp and Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Stp and Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Stp will have a positive (+) charge and travel towards the negative (–) terminal
Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Stp will have a negative (–) charge and travel towards the positive (+) terminal
Chy will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

20a9_e732

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ribonuclease A (RibA)9.313.7
Trypsin (Tryp)10.823.5

Both protein samples are placed into a gel with a constant pH of 10.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.0?

Both RibA and Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both RibA and Tryp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect RibA will have a positive (+) charge and travel towards the negative (–) terminal
Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect RibA will have a negative (–) charge and travel towards the positive (+) terminal
Tryp will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

80c3_e669

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Myelin Basic Protein (MBP)9.518.0
Estrogen Binding Protein (EBP)4.9115.0

Both protein samples are placed into a gel with a constant pH of 10.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.5?

Both MBP and EBP will have a negative (–) charge and travel towards the positive (+) terminal Correct Both MBP and EBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MBP will have a positive (+) charge and travel towards the negative (–) terminal
EBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect MBP will have a negative (–) charge and travel towards the positive (+) terminal
EBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

7001_8d61

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Cobra Venom Factor (CVF)5.2149.0
Fumerase (Fum)7.648.5

Both protein samples are placed into a gel with a constant pH of 4.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.0?

Both CVF and Fum will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both CVF and Fum will have a positive (+) charge and travel towards the negative (–) terminal Correct CVF will have a positive (+) charge and travel towards the negative (–) terminal
Fum will have a negative (–) charge and travel towards the positive (+) terminal Incorrect CVF will have a negative (–) charge and travel towards the positive (+) terminal
Fum will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

964b_ac61

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Fumerase (Fum)7.648.5
Pepsin (Pep)1.034.5

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both Fum and Pep will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Fum and Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Fum will have a positive (+) charge and travel towards the negative (–) terminal
Pep will have a negative (–) charge and travel towards the positive (+) terminal Correct Fum will have a negative (–) charge and travel towards the positive (+) terminal
Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

b8ca_8c17

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Phospholypase (Pho)10.519.5
Serine Protease (Ser)4.122.0

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both Pho and Ser will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Pho and Ser will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Pho will have a positive (+) charge and travel towards the negative (–) terminal
Ser will have a negative (–) charge and travel towards the positive (+) terminal Correct Pho will have a negative (–) charge and travel towards the positive (+) terminal
Ser will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

3296_e802

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Chymotrypsin (Chy)8.425.0
Actin (Act)5.343.0

Both protein samples are placed into a gel with a constant pH of 4.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.0?

Both Chy and Act will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Chy and Act will have a positive (+) charge and travel towards the negative (–) terminal Correct Chy will have a positive (+) charge and travel towards the negative (–) terminal
Act will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Chy will have a negative (–) charge and travel towards the positive (+) terminal
Act will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

548d_b606

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lysozyme (Lys)11.014.4
β-Galactosidase (Gal)4.6175.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Lys and Gal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lys and Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lys will have a positive (+) charge and travel towards the negative (–) terminal
Gal will have a negative (–) charge and travel towards the positive (+) terminal Correct Lys will have a negative (–) charge and travel towards the positive (+) terminal
Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

0c09_f9a1

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
G3P Dehydrogenase (GDH)8.336.0
Cytochrome c (Cyt)10.213.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both GDH and Cyt will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both GDH and Cyt will have a positive (+) charge and travel towards the negative (–) terminal Correct GDH will have a positive (+) charge and travel towards the negative (–) terminal
Cyt will have a negative (–) charge and travel towards the positive (+) terminal Incorrect GDH will have a negative (–) charge and travel towards the positive (+) terminal
Cyt will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

2727_b9db

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Tropomyosin (Trop)5.135.0
Elastase II (Ela)8.526.5

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Trop and Ela will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Trop and Ela will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Trop will have a positive (+) charge and travel towards the negative (–) terminal
Ela will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Trop will have a negative (–) charge and travel towards the positive (+) terminal
Ela will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

4482_1c8e

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
G3P Dehydrogenase (GDH)8.336.0
Heat Shock Protein (HSP)5.570.1

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both GDH and HSP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both GDH and HSP will have a positive (+) charge and travel towards the negative (–) terminal Correct GDH will have a positive (+) charge and travel towards the negative (–) terminal
HSP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect GDH will have a negative (–) charge and travel towards the positive (+) terminal
HSP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

3830_5159

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Salmine (Sal)12.13.0
Xylosidase (Xyl)5.0100.0

Both protein samples are placed into a gel with a constant pH of 4.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.0?

Both Sal and Xyl will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Sal and Xyl will have a positive (+) charge and travel towards the negative (–) terminal Correct Sal will have a positive (+) charge and travel towards the negative (–) terminal
Xyl will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Sal will have a negative (–) charge and travel towards the positive (+) terminal
Xyl will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

1b28_6083

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
SARS-CoV-2 Spike Protein (CoV)6.2149.9
Lactalbumin (Lac)4.313.0

Both protein samples are placed into a gel with a constant pH of 5.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.0?

Both CoV and Lac will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both CoV and Lac will have a positive (+) charge and travel towards the negative (–) terminal Incorrect CoV will have a positive (+) charge and travel towards the negative (–) terminal
Lac will have a negative (–) charge and travel towards the positive (+) terminal Correct CoV will have a negative (–) charge and travel towards the positive (+) terminal
Lac will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

be6b_73f6

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Serine Protease (Ser)4.122.0
Enolase (Eno)8.442.5

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Ser and Eno will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ser and Eno will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ser will have a positive (+) charge and travel towards the negative (–) terminal
Eno will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ser will have a negative (–) charge and travel towards the positive (+) terminal
Eno will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

9e68_f089

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Pyruvate Kinase (PK)5.660.0
G3P Dehydrogenase (GDH)8.336.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both PK and GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both PK and GDH will have a positive (+) charge and travel towards the negative (–) terminal Incorrect PK will have a positive (+) charge and travel towards the negative (–) terminal
GDH will have a negative (–) charge and travel towards the positive (+) terminal Incorrect PK will have a negative (–) charge and travel towards the positive (+) terminal
GDH will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

4766_4461

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Myoglobin (Myo)7.018.0
Estrogen Binding Protein (EBP)4.9115.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Myo and EBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Myo and EBP will have a positive (+) charge and travel towards the negative (–) terminal Correct Myo will have a positive (+) charge and travel towards the negative (–) terminal
EBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Myo will have a negative (–) charge and travel towards the positive (+) terminal
EBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

c56a_a1aa

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Insulin (Ins)5.45.8
Hemoglobin (Hem)7.116.7

Both protein samples are placed into a gel with a constant pH of 4.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.0?

Both Ins and Hem will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ins and Hem will have a positive (+) charge and travel towards the negative (–) terminal Correct Ins will have a positive (+) charge and travel towards the negative (–) terminal
Hem will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ins will have a negative (–) charge and travel towards the positive (+) terminal
Hem will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

42d4_56a7

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Heat Shock Protein (HSP)5.570.1
Phospholypase (Pho)10.519.5

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both HSP and Pho will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both HSP and Pho will have a positive (+) charge and travel towards the negative (–) terminal Incorrect HSP will have a positive (+) charge and travel towards the negative (–) terminal
Pho will have a negative (–) charge and travel towards the positive (+) terminal Incorrect HSP will have a negative (–) charge and travel towards the positive (+) terminal
Pho will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

5b85_dccc

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Serine Protease (Ser)4.122.0
DNA Ligase (Lig)6.060.0

Both protein samples are placed into a gel with a constant pH of 3.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.0?

Both Ser and Lig will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ser and Lig will have a positive (+) charge and travel towards the negative (–) terminal Correct Ser will have a positive (+) charge and travel towards the negative (–) terminal
Lig will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Ser will have a negative (–) charge and travel towards the positive (+) terminal
Lig will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

2c49_784c

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Serum Albumin (Alb)4.966.2
Hemoglobin (Hem)7.116.7

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Alb and Hem will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Alb and Hem will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Alb will have a positive (+) charge and travel towards the negative (–) terminal
Hem will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Alb will have a negative (–) charge and travel towards the positive (+) terminal
Hem will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

71aa_d819

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
SARS-CoV-2 Spike Protein (CoV)6.2149.9
Trypsin (Tryp)10.823.5

Both protein samples are placed into a gel with a constant pH of 5.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.0?

Both CoV and Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both CoV and Tryp will have a positive (+) charge and travel towards the negative (–) terminal Correct CoV will have a positive (+) charge and travel towards the negative (–) terminal
Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect CoV will have a negative (–) charge and travel towards the positive (+) terminal
Tryp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

8ed7_3b36

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lipase (Lip)4.740.0
Myoglobin (Myo)7.018.0

Both protein samples are placed into a gel with a constant pH of 6.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 6.0?

Both Lip and Myo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lip and Myo will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lip will have a positive (+) charge and travel towards the negative (–) terminal
Myo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lip will have a negative (–) charge and travel towards the positive (+) terminal
Myo will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

b46c_95df

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lactalbumin (Lac)4.313.0
Pepsin (Pep)1.034.5

Both protein samples are placed into a gel with a constant pH of 2.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 2.5?

Both Lac and Pep will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lac and Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lac will have a positive (+) charge and travel towards the negative (–) terminal
Pep will have a negative (–) charge and travel towards the positive (+) terminal Correct Lac will have a negative (–) charge and travel towards the positive (+) terminal
Pep will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

b3aa_3e96

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Trypsin (Tryp)10.823.5
Estrogen Binding Protein (EBP)4.9115.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Tryp and EBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Tryp and EBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Tryp will have a positive (+) charge and travel towards the negative (–) terminal
EBP will have a negative (–) charge and travel towards the positive (+) terminal Correct Tryp will have a negative (–) charge and travel towards the positive (+) terminal
EBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

fbd3_f7d1

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Immunoglobulin (IgG)7.3145.0
Neuraminidase (Neu)5.250.0

Both protein samples are placed into a gel with a constant pH of 4.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.0?

Both IgG and Neu will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both IgG and Neu will have a positive (+) charge and travel towards the negative (–) terminal Correct IgG will have a positive (+) charge and travel towards the negative (–) terminal
Neu will have a negative (–) charge and travel towards the positive (+) terminal Incorrect IgG will have a negative (–) charge and travel towards the positive (+) terminal
Neu will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

65fa_4149

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Immunoglobulin (IgG)7.3145.0
Horseradish peroxidase (HP)9.034.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both IgG and HP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both IgG and HP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect IgG will have a positive (+) charge and travel towards the negative (–) terminal
HP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect IgG will have a negative (–) charge and travel towards the positive (+) terminal
HP will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

512e_af54

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
DNA Ligase (Lig)6.060.0
Fumerase (Fum)7.648.5

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Lig and Fum will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lig and Fum will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lig will have a positive (+) charge and travel towards the negative (–) terminal
Fum will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lig will have a negative (–) charge and travel towards the positive (+) terminal
Fum will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

3db2_254d

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
α-Amylase (Amy)5.961.0
Trypsin (Tryp)10.823.5

Both protein samples are placed into a gel with a constant pH of 12.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 12.0?

Both Amy and Tryp will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Amy and Tryp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Amy will have a positive (+) charge and travel towards the negative (–) terminal
Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Amy will have a negative (–) charge and travel towards the positive (+) terminal
Tryp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

6c50_9495

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Cobra Venom Factor (CVF)5.2149.0
Horseradish peroxidase (HP)9.034.0

Both protein samples are placed into a gel with a constant pH of 10.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.0?

Both CVF and HP will have a negative (–) charge and travel towards the positive (+) terminal Correct Both CVF and HP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect CVF will have a positive (+) charge and travel towards the negative (–) terminal
HP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect CVF will have a negative (–) charge and travel towards the positive (+) terminal
HP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

a8fc_3c9e

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lysozyme (Lys)11.014.4
Casein (Cas)4.924.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Lys and Cas will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lys and Cas will have a positive (+) charge and travel towards the negative (–) terminal Correct Lys will have a positive (+) charge and travel towards the negative (–) terminal
Cas will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lys will have a negative (–) charge and travel towards the positive (+) terminal
Cas will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

abec_29f9

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
DNA Ligase (Lig)6.060.0
Histone (His)10.815.0

Both protein samples are placed into a gel with a constant pH of 8.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.5?

Both Lig and His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lig and His will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Lig will have a positive (+) charge and travel towards the negative (–) terminal
His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lig will have a negative (–) charge and travel towards the positive (+) terminal
His will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

7b14_d1a7

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Succinate Ligase (SL)6.620.9
Aprotinin (Apr)9.26.5

Both protein samples are placed into a gel with a constant pH of 10.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 10.5?

Both SL and Apr will have a negative (–) charge and travel towards the positive (+) terminal Correct Both SL and Apr will have a positive (+) charge and travel towards the negative (–) terminal Incorrect SL will have a positive (+) charge and travel towards the negative (–) terminal
Apr will have a negative (–) charge and travel towards the positive (+) terminal Incorrect SL will have a negative (–) charge and travel towards the positive (+) terminal
Apr will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

041f_0b93

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Ribonuclease A (RibA)9.313.7
Streptavidin (Stp)6.013.2

Both protein samples are placed into a gel with a constant pH of 5.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.0?

Both RibA and Stp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both RibA and Stp will have a positive (+) charge and travel towards the negative (–) terminal Correct RibA will have a positive (+) charge and travel towards the negative (–) terminal
Stp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect RibA will have a negative (–) charge and travel towards the positive (+) terminal
Stp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

c154_229b

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Elastase II (Ela)8.526.5
Xylosidase (Xyl)5.0100.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Ela and Xyl will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Ela and Xyl will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Ela will have a positive (+) charge and travel towards the negative (–) terminal
Xyl will have a negative (–) charge and travel towards the positive (+) terminal Correct Ela will have a negative (–) charge and travel towards the positive (+) terminal
Xyl will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

ab1d_18f7

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Catalase (Cat)6.765.5
Histone (His)10.815.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both Cat and His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Cat and His will have a positive (+) charge and travel towards the negative (–) terminal Correct Cat will have a positive (+) charge and travel towards the negative (–) terminal
His will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cat will have a negative (–) charge and travel towards the positive (+) terminal
His will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

580f_9eaf

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Histone (His)10.815.0
Fibrinogen (Fib)5.863.5

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both His and Fib will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both His and Fib will have a positive (+) charge and travel towards the negative (–) terminal Correct His will have a positive (+) charge and travel towards the negative (–) terminal
Fib will have a negative (–) charge and travel towards the positive (+) terminal Incorrect His will have a negative (–) charge and travel towards the positive (+) terminal
Fib will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

54ba_de2a

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Salmine (Sal)12.13.0
SARS-CoV-2 Spike Protein (CoV)6.2149.9

Both protein samples are placed into a gel with a constant pH of 9.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.0?

Both Sal and CoV will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Sal and CoV will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Sal will have a positive (+) charge and travel towards the negative (–) terminal
CoV will have a negative (–) charge and travel towards the positive (+) terminal Correct Sal will have a negative (–) charge and travel towards the positive (+) terminal
CoV will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

a176_ed7f

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
SARS-CoV-2 Spike Protein (CoV)6.2149.9
Aprotinin (Apr)9.26.5

Both protein samples are placed into a gel with a constant pH of 7.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.5?

Both CoV and Apr will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both CoV and Apr will have a positive (+) charge and travel towards the negative (–) terminal Incorrect CoV will have a positive (+) charge and travel towards the negative (–) terminal
Apr will have a negative (–) charge and travel towards the positive (+) terminal Incorrect CoV will have a negative (–) charge and travel towards the positive (+) terminal
Apr will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

34a2_3c9d

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Fibrinogen (Fib)5.863.5
Ribonuclease A (RibA)9.313.7

Both protein samples are placed into a gel with a constant pH of 4.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 4.5?

Both Fib and RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Fib and RibA will have a positive (+) charge and travel towards the negative (–) terminal Correct Fib will have a positive (+) charge and travel towards the negative (–) terminal
RibA will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Fib will have a negative (–) charge and travel towards the positive (+) terminal
RibA will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

9a68_6af7

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Estrogen Binding Protein (EBP)4.9115.0
Myelin Basic Protein (MBP)9.518.0

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both EBP and MBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both EBP and MBP will have a positive (+) charge and travel towards the negative (–) terminal Correct EBP will have a positive (+) charge and travel towards the negative (–) terminal
MBP will have a negative (–) charge and travel towards the positive (+) terminal Incorrect EBP will have a negative (–) charge and travel towards the positive (+) terminal
MBP will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

b455_b883

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Casein (Cas)4.924.0
Trypsin (Tryp)10.823.5

Both protein samples are placed into a gel with a constant pH of 3.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.5?

Both Cas and Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Cas and Tryp will have a positive (+) charge and travel towards the negative (–) terminal Correct Cas will have a positive (+) charge and travel towards the negative (–) terminal
Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cas will have a negative (–) charge and travel towards the positive (+) terminal
Tryp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

a531_814c

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Catalase (Cat)6.765.5
β-Galactosidase (Gal)4.6175.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Cat and Gal will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Cat and Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Cat will have a positive (+) charge and travel towards the negative (–) terminal
Gal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cat will have a negative (–) charge and travel towards the positive (+) terminal
Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

ea58_868e

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Tropomyosin (Trop)5.135.0
γ-Globulin (Glob)6.657.0

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Trop and Glob will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Trop and Glob will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Trop will have a positive (+) charge and travel towards the negative (–) terminal
Glob will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Trop will have a negative (–) charge and travel towards the positive (+) terminal
Glob will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

dfe0_6748

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Catalase (Cat)6.765.5
Trypsin (Tryp)10.823.5

Both protein samples are placed into a gel with a constant pH of 9.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.0?

Both Cat and Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Cat and Tryp will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Cat will have a positive (+) charge and travel towards the negative (–) terminal
Tryp will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Cat will have a negative (–) charge and travel towards the positive (+) terminal
Tryp will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

a64e_70fa

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
β-Galactosidase (Gal)4.6175.0
Sucrase (Suc)6.551.0

Both protein samples are placed into a gel with a constant pH of 5.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 5.5?

Both Gal and Suc will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Gal and Suc will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Gal will have a positive (+) charge and travel towards the negative (–) terminal
Suc will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Gal will have a negative (–) charge and travel towards the positive (+) terminal
Suc will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

6570_14d3

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Chymotrypsin (Chy)8.425.0
β-Galactosidase (Gal)4.6175.0

Both protein samples are placed into a gel with a constant pH of 9.5. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 9.5?

Both Chy and Gal will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Chy and Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Chy will have a positive (+) charge and travel towards the negative (–) terminal
Gal will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Chy will have a negative (–) charge and travel towards the positive (+) terminal
Gal will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

ed11_a967

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Lactalbumin (Lac)4.313.0
Fibrinogen (Fib)5.863.5

Both protein samples are placed into a gel with a constant pH of 3.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 3.0?

Both Lac and Fib will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Lac and Fib will have a positive (+) charge and travel towards the negative (–) terminal Correct Lac will have a positive (+) charge and travel towards the negative (–) terminal
Fib will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Lac will have a negative (–) charge and travel towards the positive (+) terminal
Fib will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

c2b3_9ea0

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Trypsin (Tryp)10.823.5
Insulin (Ins)5.45.8

Both protein samples are placed into a gel with a constant pH of 8.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 8.0?

Both Tryp and Ins will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Tryp and Ins will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Tryp will have a positive (+) charge and travel towards the negative (–) terminal
Ins will have a negative (–) charge and travel towards the positive (+) terminal Correct Tryp will have a negative (–) charge and travel towards the positive (+) terminal
Ins will have a positive (+) charge and travel towards the negative (–) terminal Incorrect MC

a8f5_d84a

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Pyruvate Kinase (PK)5.660.0
Chymotrypsin (Chy)8.425.0

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both PK and Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both PK and Chy will have a positive (+) charge and travel towards the negative (–) terminal Incorrect PK will have a positive (+) charge and travel towards the negative (–) terminal
Chy will have a negative (–) charge and travel towards the positive (+) terminal Incorrect PK will have a negative (–) charge and travel towards the positive (+) terminal
Chy will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

9142_3592

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Luciferase (Luc)5.750.0
Aldolase (Aldo)8.547.5

Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 7.0?

Both Luc and Aldo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Both Luc and Aldo will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Luc will have a positive (+) charge and travel towards the negative (–) terminal
Aldo will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Luc will have a negative (–) charge and travel towards the positive (+) terminal
Aldo will have a positive (+) charge and travel towards the negative (–) terminal Correct MC

2181_48ea

Isoelectric Point Problem

A protein's isoelectric point (pI) is the pH at which it carries no net charge. When placed in a pH environment different from its pI, the protein will acquire a net charge and migrate in an electric field accordingly.

A mixture of two proteins are to be separated by isoelectric focusing.

Protein
Name
Isoelectric
Point (pI)
Molecular
Weight
Hemoglobin (Hem)7.116.7
Lysozyme (Lys)11.014.4

Both protein samples are placed into a gel with a constant pH of 12.0. The gel is then placed into an electric field.

In which direction will each protein in the table migrate at pH 12.0?

Both Hem and Lys will have a negative (–) charge and travel towards the positive (+) terminal Correct Both Hem and Lys will have a positive (+) charge and travel towards the negative (–) terminal Incorrect Hem will have a positive (+) charge and travel towards the negative (–) terminal
Lys will have a negative (–) charge and travel towards the positive (+) terminal Incorrect Hem will have a negative (–) charge and travel towards the positive (+) terminal
Lys will have a positive (+) charge and travel towards the negative (–) terminal Incorrect