MA

5c3b_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. PdiI is derived from Pseudomonas diminuta Mck 33-321, and XbaI is derived from Xanthomonas badrii. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0PdiI 1XbaI 2PdiI 3 4 5 6 7XbaI 8 9PdiI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PdiI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

6b12_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. AscI is derived from Arthrobacter species, and EheI is derived from Erwinia herbicola 9/5. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1AscI 2EheI 3AscI 4 5 6 7 8EheI 9 10AscI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AscI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

4df1_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. ChaI is derived from Corynebacterium halofytica, and TagI is derived from Thermocrispum agreste. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0ChaI 1TagI 2 3ChaI 4TagI 5 6 7 8 9ChaI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ChaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

6e7d_3635

Restriction enzymes are proteins that cut DNA at specific sequences. FspI is derived from Fischerella species, and SmaI is derived from Serratia marcescens Sb. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1FspI 2 3SmaI 4FspI 5SmaI 6 7 8FspI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with FspI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

a13a_3635

Restriction enzymes are proteins that cut DNA at specific sequences. NcoI is derived from Nocardia corallina, and AfeI is derived from Alcaligenes faecalis T2774. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2NcoI 3 4 5AfeI 6NcoI 7 8AfeI 9NcoI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NcoI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

b2da_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. ChaI is derived from Corynebacterium halofytica, and MvnI is derived from Methanococcus vannielii. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1ChaI 2 3 4 5MvnI 6ChaI 7MvnI 8 9ChaI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ChaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

38a1_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. PdiI is derived from Pseudomonas diminuta Mck 33-321, and AanI is derived from Arthrobacter aurescens RFL2. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1PdiI 2 3AanI 4PdiI 5 6 7AanI 8 9PdiI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PdiI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

d878_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. RgaI is derived from Rhizobium galegae, and PagI is derived from Pseudomonas alcaligenes Sau 14-027. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0RgaI 1 2PagI 3 4RgaI 5 6 7 8PagI 9RgaI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with RgaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

2ccc_3635

Restriction enzymes are proteins that cut DNA at specific sequences. SalI is derived from Streptomyces albus G, and PagI is derived from Pseudomonas alcaligenes Sau 14-027. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1SalI 2 3PagI 4SalI 5PagI 6 7 8SalI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SalI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

2661_3635

Restriction enzymes are proteins that cut DNA at specific sequences. FbaI is derived from Flavobacterium balustinum, and KroI is derived from Kocurea rosea 307. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3FbaI 4 5KroI 6FbaI 7KroI 8 9 10FbaI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with FbaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

fdc6_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. AfeI is derived from Alcaligenes faecalis T2774, and TaqI is derived from Thermus aquaticus YTI. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0AfeI 1TaqI 2 3 4 5AfeI 6 7 8TaqI 9AfeI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AfeI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

5078_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SrfI is derived from Streptomyces species Srf, and NheI is derived from Neisseria mucosa. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1SrfI 2NheI 3 4 5SrfI 6 7 8NheI 9 10SrfI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SrfI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

5efd_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. ApaI is derived from Acetobacter pasteurianus sub. pasteurianus, and RruI is derived from Rhodococcus ruber RFL5. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1ApaI 2RruI 3ApaI 4 5 6 7 8RruI 9 10ApaI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ApaI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

ca85_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. NdeI is derived from Neisseria denitrificans, and SfoI is derived from Serratia fonticola. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2NdeI 3 4 5 6SfoI 7NdeI 8SfoI 9 10NdeI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NdeI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

430c_3635

Restriction enzymes are proteins that cut DNA at specific sequences. RsaI is derived from Rhodopseudomonas sphaeroides, and NaeI is derived from Nocardia aerocolonigenes. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0RsaI 1NaeI 2RsaI 3NaeI 4 5 6RsaI 7 8 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with RsaI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Incorrect MA

8fc0_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. MlsI is derived from Micrococcus luteus Ng 16-122, and AclI is derived from Acinetobacter calcoaceticus M4. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0MlsI 1AclI 2 3MlsI 4AclI 5 6 7 8 9MlsI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MlsI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

95a9_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SplI is derived from Spirulina platensis, and VneI is derived from Vibrio nereis 18. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1SplI 2 3 4VneI 5 6SplI 7VneI 8SplI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SplI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

a080_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. MfeI is derived from Mycoplasma fermentans, and VspI is derived from Vibrio species 343. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0MfeI 1VspI 2 3MfeI 4VspI 5 6 7 8MfeI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MfeI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

884f_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SwaI is derived from Staphylococcus warneri, and VneI is derived from Vibrio nereis 18. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1SwaI 2 3VneI 4 5 6SwaI 7VneI 8SwaI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SwaI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

e3db_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. PauI is derived from Paracoccus alcaliphilus ZVK3-3, and DraI is derived from Deinococcus radiophilus. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3PauI 4 5 6DraI 7 8PauI 9DraI 10PauI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PauI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

5471_3635

Restriction enzymes are proteins that cut DNA at specific sequences. MseI is derived from Micrococcus species, and BsnI is derived from Bacillus species SJN7. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3 4MseI 5BsnI 6MseI 7 8BsnI 9MseI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MseI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect MA

2e74_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. XspI is derived from Xanthomonas species YK1, and TaqI is derived from Thermus aquaticus YTI. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0XspI 1TaqI 2 3XspI 4 5TaqI 6 7 8 9 10XspI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with XspI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

233b_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. FseI is derived from Frankia species Eul1b, and VspI is derived from Vibrio species 343. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1FseI 2 3 4VspI 5FseI 6 7 8 9VspI 10FseI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with FseI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

38fe_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. FbaI is derived from Flavobacterium balustinum, and MreI is derived from Micromonospora rhodorangea RFL1. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0FbaI 1MreI 2 3 4FbaI 5 6 7 8 9MreI 10FbaI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with FbaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

ce90_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. HhaI is derived from Haemophilus haemolyticus, and AcvI is derived from Aeromonas caviae. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2HhaI 3AcvI 4HhaI 5 6AcvI 7 8 9HhaI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with HhaI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

ed1b_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. ApaI is derived from Acetobacter pasteurianus sub. pasteurianus, and SacI is derived from Streptomyces achromogenes. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0ApaI 1 2SacI 3 4ApaI 5SacI 6 7 8 9 10ApaI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ApaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

f76d_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. ZrmI is derived from Zoogloea ramigera SCA, and PdiI is derived from Pseudomonas diminuta Mck 33-321. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1ZrmI 2 3 4 5PdiI 6 7ZrmI 8 9PdiI 10ZrmI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ZrmI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

e778_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. DinI is derived from Deinococcus indicus, and MroI is derived from Micrococcus roseus. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2DinI 3MroI 4 5DinI 6 7 8 9MroI 10DinI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with DinI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

e5ba_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. CfoI is derived from Clostridium formicoaceticum, and SlaI is derived from Streptomyces lavendulae. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0CfoI 1 2 3 4SlaI 5 6 7CfoI 8SlaI 9CfoI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with CfoI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

1a30_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. SlaI is derived from Streptomyces lavendulae, and MalI is derived from Marinococus albus I. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0SlaI 1 2 3 4MalI 5 6 7SlaI 8 9MalI 10SlaI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SlaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

530c_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. SpeI is derived from Sphaerotilus natans, and NaeI is derived from Nocardia aerocolonigenes. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1SpeI 2 3NaeI 4SpeI 5NaeI 6 7 8 9 10SpeI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SpeI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

1ead_3635

Restriction enzymes are proteins that cut DNA at specific sequences. NdeI is derived from Neisseria denitrificans, and MstI is derived from Microcoleus species. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2NdeI 3 4MstI 5 6NdeI 7MstI 8NdeI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NdeI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Incorrect MA

6724_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. AcvI is derived from Aeromonas caviae, and PagI is derived from Pseudomonas alcaligenes Sau 14-027. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0AcvI 1 2 3 4 5PagI 6 7AcvI 8 9PagI 10AcvI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AcvI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

f31d_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. BmtI is derived from Bacillus megaterium S2, and TagI is derived from Thermocrispum agreste. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0BmtI 1 2 3 4TagI 5 6BmtI 7TagI 8BmtI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BmtI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

dce6_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. AclI is derived from Acinetobacter calcoaceticus M4, and FseI is derived from Frankia species Eul1b. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0AclI 1FseI 2 3AclI 4FseI 5 6 7 8 9AclI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AclI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

e5e6_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. KasI is derived from Kluyvera ascorbata, and BsnI is derived from Bacillus species SJN7. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0KasI 1BsnI 2 3 4 5KasI 6 7 8BsnI 9KasI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with KasI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

d040_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. KpnI is derived from Klebsiella pneumoniae OK8, and MunI is derived from Mycoplasma species. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2KpnI 3 4 5 6MunI 7 8KpnI 9MunI 10KpnI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with KpnI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

9323_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. PagI is derived from Pseudomonas alcaligenes Sau 14-027, and MscI is derived from Micrococcus species. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2PagI 3 4MscI 5 6 7 8PagI 9MscI 10PagI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PagI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

3583_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. BfrI is derived from Bacteroides fragilis, and TaqI is derived from Thermus aquaticus YTI. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1BfrI 2 3 4 5TaqI 6BfrI 7TaqI 8BfrI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BfrI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

15fb_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. KroI is derived from Kocurea rosea 307, and XmaI is derived from Xanthomonas malvacearum. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0KroI 1XmaI 2 3 4 5 6KroI 7 8XmaI 9 10KroI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with KroI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

a75c_3635

Restriction enzymes are proteins that cut DNA at specific sequences. StuI is derived from Streptomyces tubercidicus, and KroI is derived from Kocurea rosea 307. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3StuI 4 5KroI 6StuI 7KroI 8StuI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with StuI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect MA

41fe_3635

Restriction enzymes are proteins that cut DNA at specific sequences. SfoI is derived from Serratia fonticola, and TagI is derived from Thermocrispum agreste. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1SfoI 2 3TagI 4SfoI 5 6 7TagI 8SfoI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SfoI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

c731_3635

Restriction enzymes are proteins that cut DNA at specific sequences. SwaI is derived from Staphylococcus warneri, and BalI is derived from Brevibacterium albidum. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3SwaI 4BalI 5 6SwaI 7BalI 8SwaI 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SwaI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect MA

0bce_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. AfaI is derived from Acidiphilium facilis 28H, and MroI is derived from Micrococcus roseus. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0AfaI 1 2 3 4MroI 5 6AfaI 7MroI 8 9AfaI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AfaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

ef10_3635

Restriction enzymes are proteins that cut DNA at specific sequences. PciI is derived from Planococcus citreus SE-F45, and NaeI is derived from Nocardia aerocolonigenes. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3PciI 4 5 6NaeI 7PciI 8NaeI 9 10PciI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PciI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

c3c9_3635

Restriction enzymes are proteins that cut DNA at specific sequences. MseI is derived from Micrococcus species, and SgfI is derived from Streptomyces griseoruber. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1MseI 2SgfI 3MseI 4 5SgfI 6MseI 7 8 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MseI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect MA

9afb_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SalI is derived from Streptomyces albus G, and BalI is derived from Brevibacterium albidum. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0SalI 1BalI 2 3 4SalI 5BalI 6 7 8 9SalI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SalI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

ea54_3635

Restriction enzymes are proteins that cut DNA at specific sequences. TaiI is derived from Thermus aquaticus cs1-331, and AluI is derived from Arthrobacter luteus. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3 4TaiI 5AluI 6TaiI 7AluI 8 9TaiI 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with TaiI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect MA

a164_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. GlaI is derived from Glacial ice bacterium GL29, and DinI is derived from Deinococcus indicus. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0GlaI 1DinI 2 3GlaI 4 5 6 7DinI 8 9 10GlaI 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with GlaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

bde4_3635

Restriction enzymes are proteins that cut DNA at specific sequences. AfaI is derived from Acidiphilium facilis 28H, and PmeI is derived from Pseudomonas mendocina. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2AfaI 3PmeI 4 5AfaI 6PmeI 7AfaI 8 9 10 11 12Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AfaI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect