MA

2d8e_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. KspI is derived from Kluyvera species, and PscI is derived from Paracoccus species RFL1. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0KspI 1 2PscI 3 4 5 6KspI 7PscI 8KspI 9 10PscI 11 12 13 14KspI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with KspI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

96d1_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SmiI is derived from Streptococcus milleri S, and MluI is derived from Micrococcus luteus. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2SmiI 3 4MluI 5 6 7SmiI 8 9MluI 10SmiI 11MluI 12 13SmiI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SmiI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

ac95_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. AbsI is derived from Arthrobacter species 7M06, and VspI is derived from Vibrio species 343. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2AbsI 3 4VspI 5 6 7 8 9AbsI 10VspI 11AbsI 12VspI 13AbsI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AbsI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

3433_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SacI is derived from Streptomyces achromogenes, and PauI is derived from Paracoccus alcaliphilus ZVK3-3. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1SacI 2PauI 3 4 5SacI 6 7 8 9PauI 10SacI 11PauI 12 13 14SacI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SacI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

53bd_3635

Restriction enzymes are proteins that cut DNA at specific sequences. ChaI is derived from Corynebacterium halofytica, and EagI is derived from Enterobacter agglomerans. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3 4ChaI 5EagI 6ChaI 7 8 9EagI 10ChaI 11 12EagI 13 14ChaI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ChaI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Incorrect MA

0c4f_3635

Restriction enzymes are proteins that cut DNA at specific sequences. MssI is derived from Methylobacterium species Dd 5-732, and SdaI is derived from Streptomyces diastaticus Ng7-324. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0MssI 1SdaI 2 3MssI 4SdaI 5 6 7MssI 8 9 10SdaI 11MssI 12 13 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MssI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

e44a_3635

Restriction enzymes are proteins that cut DNA at specific sequences. AseI is derived from Aquaspirillum serpens, and FaeI is derived from Flavobacterium aquatile N3. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3AseI 4 5 6FaeI 7AseI 8 9FaeI 10AseI 11 12FaeI 13 14AseI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AseI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

8a80_d233

Restriction enzymes are proteins that cut DNA at specific sequences. AscI is derived from Arthrobacter species, and PauI is derived from Paracoccus alcaliphilus ZVK3-3. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0AscI 1PauI 2 3AscI 4 5 6PauI 7 8 9 10 11AscI 12 13PauI 14AscI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AscI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Incorrect 8 kb Correct 9 kb Incorrect MA

4a52_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. AgeI is derived from Agrobacterium gelatinovorum, and SciI is derived from Streptoverticillium cinnamoneum. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0AgeI 1SciI 2AgeI 3 4SciI 5 6 7 8AgeI 9 10 11 12 13SciI 14AgeI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AgeI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

d7f2_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. PmeI is derived from Pseudomonas mendocina, and MstI is derived from Microcoleus species. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1PmeI 2 3 4 5MstI 6PmeI 7 8 9MstI 10 11PmeI 12MstI 13 14PmeI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PmeI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

2f1c_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. SfoI is derived from Serratia fonticola, and DinI is derived from Deinococcus indicus. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0SfoI 1 2 3DinI 4SfoI 5DinI 6 7 8 9 10SfoI 11 12DinI 13 14SfoI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SfoI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

df10_3635

Restriction enzymes are proteins that cut DNA at specific sequences. AcvI is derived from Aeromonas caviae, and MvnI is derived from Methanococcus vannielii. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2AcvI 3MvnI 4 5 6AcvI 7MvnI 8AcvI 9 10MvnI 11 12AcvI 13 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AcvI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Incorrect MA

e9a0_3635

Restriction enzymes are proteins that cut DNA at specific sequences. AclI is derived from Acinetobacter calcoaceticus M4, and RgaI is derived from Rhizobium galegae. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3 4 5AclI 6RgaI 7 8AclI 9RgaI 10AclI 11 12RgaI 13AclI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AclI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect MA

54a0_d233

Restriction enzymes are proteins that cut DNA at specific sequences. NotI is derived from Nocardia otitidis-caviarum, and ChaI is derived from Corynebacterium halofytica. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0NotI 1ChaI 2 3 4 5 6 7 8NotI 9 10ChaI 11NotI 12ChaI 13 14NotI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NotI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Incorrect 8 kb Correct 9 kb Incorrect MA

b0ad_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. BsnI is derived from Bacillus species SJN7, and FspI is derived from Fischerella species. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1BsnI 2 3 4 5 6 7FspI 8BsnI 9FspI 10 11BsnI 12FspI 13 14BsnI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BsnI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

e851_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. EgeI is derived from Enterobacter gergoviae NA, and MluI is derived from Micrococcus luteus. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1EgeI 2MluI 3 4EgeI 5MluI 6 7EgeI 8 9 10 11MluI 12 13 14EgeI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with EgeI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

21bb_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. CfoI is derived from Clostridium formicoaceticum, and FaeI is derived from Flavobacterium aquatile N3. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0CfoI 1FaeI 2 3 4 5CfoI 6 7 8FaeI 9 10CfoI 11 12 13FaeI 14CfoI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with CfoI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

346e_3635

Restriction enzymes are proteins that cut DNA at specific sequences. MstI is derived from Microcoleus species, and KspI is derived from Kluyvera species. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3MstI 4KspI 5 6MstI 7 8KspI 9MstI 10KspI 11 12 13MstI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MstI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

2d8d_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. MstI is derived from Microcoleus species, and VneI is derived from Vibrio nereis 18. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1MstI 2 3VneI 4 5 6MstI 7VneI 8 9MstI 10 11VneI 12 13 14MstI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MstI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

7821_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. KspI is derived from Kluyvera species, and MscI is derived from Micrococcus species. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1KspI 2 3 4MscI 5 6KspI 7 8 9MscI 10 11KspI 12 13MscI 14KspI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with KspI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

897e_3635

Restriction enzymes are proteins that cut DNA at specific sequences. EagI is derived from Enterobacter agglomerans, and KspI is derived from Kluyvera species. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3EagI 4 5KspI 6EagI 7KspI 8 9 10EagI 11 12KspI 13EagI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with EagI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

0b1a_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. AcvI is derived from Aeromonas caviae, and NaeI is derived from Nocardia aerocolonigenes. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1AcvI 2 3NaeI 4 5 6 7AcvI 8NaeI 9 10AcvI 11NaeI 12 13AcvI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AcvI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

d639_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. KasI is derived from Kluyvera ascorbata, and MssI is derived from Methylobacterium species Dd 5-732. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0KasI 1 2 3MssI 4 5KasI 6 7 8MssI 9 10KasI 11MssI 12KasI 13 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with KasI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

50c3_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. RgaI is derived from Rhizobium galegae, and VspI is derived from Vibrio species 343. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1RgaI 2 3 4 5VspI 6RgaI 7 8VspI 9 10RgaI 11 12VspI 13 14RgaI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with RgaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

417b_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. PabI is derived from Pyrococcus abyssi, and MaeI is derived from Methanococcus aeolicus PL-15/H. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0PabI 1 2 3MaeI 4 5PabI 6MaeI 7PabI 8MaeI 9PabI 10 11 12 13 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PabI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

287e_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. FaeI is derived from Flavobacterium aquatile N3, and SstI is derived from Streptomyces stanford. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2FaeI 3 4 5SstI 6 7 8 9FaeI 10SstI 11FaeI 12SstI 13FaeI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with FaeI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

e315_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. RigI is derived from Rhizobium yangligense, and KroI is derived from Kocurea rosea 307. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2RigI 3KroI 4 5 6 7RigI 8 9KroI 10 11 12RigI 13KroI 14RigI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with RigI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

e1ef_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. MscI is derived from Micrococcus species, and AhlI is derived from Alteromonas haloplanktis SP. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2MscI 3AhlI 4MscI 5AhlI 6MscI 7 8AhlI 9 10 11MscI 12 13 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MscI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

13f3_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SgfI is derived from Streptomyces griseoruber, and AfeI is derived from Alcaligenes faecalis T2774. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3 4 5SgfI 6 7 8AfeI 9 10SgfI 11AfeI 12SgfI 13AfeI 14SgfI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SgfI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

27dd_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. HpaI is derived from Haemophilus parainfluenzae, and AseI is derived from Aquaspirillum serpens. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2HpaI 3 4 5 6AseI 7 8HpaI 9 10AseI 11HpaI 12 13AseI 14HpaI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with HpaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

3741_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. SpeI is derived from Sphaerotilus natans, and MreI is derived from Micromonospora rhodorangea RFL1. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0SpeI 1MreI 2 3 4SpeI 5 6 7MreI 8 9 10SpeI 11 12 13MreI 14SpeI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SpeI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

29f7_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. EgeI is derived from Enterobacter gergoviae NA, and HhaI is derived from Haemophilus haemolyticus. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0EgeI 1HhaI 2 3EgeI 4 5HhaI 6EgeI 7 8 9 10HhaI 11EgeI 12 13 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with EgeI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

438d_3635

Restriction enzymes are proteins that cut DNA at specific sequences. NheI is derived from Neisseria mucosa, and StuI is derived from Streptomyces tubercidicus. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3NheI 4StuI 5 6NheI 7 8 9StuI 10NheI 11 12 13StuI 14NheI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NheI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

6396_3635

Restriction enzymes are proteins that cut DNA at specific sequences. ZraI is derived from Zoogloea ramigera 11, and PstI is derived from Providencia stuartii 164. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3 4ZraI 5PstI 6ZraI 7 8 9PstI 10ZraI 11 12PstI 13 14ZraI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ZraI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Incorrect MA

f6c1_1413

Restriction enzymes are proteins that cut DNA at specific sequences. AhlI is derived from Alteromonas haloplanktis SP, and BalI is derived from Brevibacterium albidum. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0AhlI 1BalI 2AhlI 3BalI 4AhlI 5BalI 6 7 8 9 10 11 12 13AhlI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AhlI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Incorrect 8 kb Incorrect 9 kb Correct 10 kb Incorrect MA

e3b7_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. EgeI is derived from Enterobacter gergoviae NA, and XmaI is derived from Xanthomonas malvacearum. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2EgeI 3XmaI 4EgeI 5XmaI 6EgeI 7 8 9XmaI 10 11 12 13EgeI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with EgeI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

0c90_3635

Restriction enzymes are proteins that cut DNA at specific sequences. MalI is derived from Marinococus albus I, and PabI is derived from Pyrococcus abyssi. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1MalI 2PabI 3 4MalI 5 6 7PabI 8MalI 9PabI 10 11 12MalI 13 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MalI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

2fb1_3635

Restriction enzymes are proteins that cut DNA at specific sequences. SphI is derived from Streptomyces phaeochromogenes, and EgeI is derived from Enterobacter gergoviae NA. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1SphI 2 3EgeI 4SphI 5EgeI 6 7 8SphI 9 10EgeI 11SphI 12 13 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SphI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

1bee_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. ChaI is derived from Corynebacterium halofytica, and BfrI is derived from Bacteroides fragilis. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1ChaI 2 3BfrI 4 5ChaI 6 7BfrI 8 9 10ChaI 11 12 13BfrI 14ChaI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ChaI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

083c_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. PaeI is derived from Pseudomonas aeruginosa, and XspI is derived from Xanthomonas species YK1. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0PaeI 1 2XspI 3 4 5 6PaeI 7XspI 8PaeI 9 10XspI 11 12 13 14PaeI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PaeI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

348f_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. MreI is derived from Micromonospora rhodorangea RFL1, and BcuI is derived from Bacillus coagulans Vs 29-022. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0MreI 1 2 3BcuI 4 5MreI 6 7 8 9BcuI 10MreI 11 12 13BcuI 14MreI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MreI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

0eb5_3635

Restriction enzymes are proteins that cut DNA at specific sequences. NsbI is derived from Neisseria subflava Va-1, and XbaI is derived from Xanthomonas badrii. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3 4NsbI 5XbaI 6 7NsbI 8XbaI 9NsbI 10 11XbaI 12NsbI 13 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NsbI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect MA

dc89_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. NruI is derived from Nocardia rubra ATCC 15906, and AclI is derived from Acinetobacter calcoaceticus M4. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0NruI 1AclI 2 3 4 5 6NruI 7 8AclI 9 10NruI 11 12 13AclI 14NruI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NruI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

9b33_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SciI is derived from Streptoverticillium cinnamoneum, and ZrmI is derived from Zoogloea ramigera SCA. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2SciI 3 4 5 6ZrmI 7SciI 8ZrmI 9 10SciI 11 12ZrmI 13SciI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SciI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

3681_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. SplI is derived from Spirulina platensis, and KasI is derived from Kluyvera ascorbata. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0SplI 1 2 3 4KasI 5 6SplI 7KasI 8 9 10SplI 11 12 13KasI 14SplI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SplI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

53dc_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. AcvI is derived from Aeromonas caviae, and BlnI is derived from Brevibacterium linens. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0AcvI 1 2 3BlnI 4 5AcvI 6BlnI 7 8 9AcvI 10 11 12BlnI 13AcvI 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AcvI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

d2e2_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. TaiI is derived from Thermus aquaticus cs1-331, and SfoI is derived from Serratia fonticola. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0TaiI 1 2SfoI 3 4 5 6TaiI 7 8 9SfoI 10 11 12TaiI 13SfoI 14TaiI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with TaiI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

856b_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SwaI is derived from Staphylococcus warneri, and MlsI is derived from Micrococcus luteus Ng 16-122. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0SwaI 1MlsI 2SwaI 3 4MlsI 5 6 7SwaI 8MlsI 9 10 11 12SwaI 13 14 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SwaI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

1701_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. HpaI is derived from Haemophilus parainfluenzae, and XspI is derived from Xanthomonas species YK1. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3HpaI 4XspI 5HpaI 6XspI 7HpaI 8 9XspI 10 11 12 13 14HpaI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with HpaI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

a410_3635

Restriction enzymes are proteins that cut DNA at specific sequences. SspI is derived from Sphaerotilus species, and CfoI is derived from Clostridium formicoaceticum. Both enzymes are labeled on the map.

DNA Strand Question:

Examine the DNA strand shown below. The table highlights a specific portion of a much longer DNA molecule to focus on the region containing restriction sites.

              0 1 2 3SspI 4CfoI 5 6 7SspI 8 9CfoI 10 11SspI 12 13CfoI 14SspI 15 16Scale in kb

Note: The dashes at both ends of the strand indicate that the next restriction site is far away and outside the visible region. Because this segment is part of a much larger molecule, any uncut or very large fragments will not travel into the gel and will appear to be stuck in the well.

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SspI only. Select all that apply.
1 kb Incorrect 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect