MC

84fa_cdbb

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + a a a a
4,152 
+ + a a + + a a
984 
+ + a a a a + +
954 
a a + + + + a a
1,015 
a a + + a a + +
959 
a a a a + + + +
3,936 
TOTAL12,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene A and its centromere.

A. ½×(954 + 984 + 1,015)/12,000 = ½×2,953/12,000 = 0.1230 = 12.30 cM
Incorrect B. ½×(959 + 1,015 + 3,936)/12,000 = ½×5,910/12,000 = 0.2462 = 24.62 cM
Incorrect C. ½×(984 + 3,936 + 4,152)/12,000 = ½×9,072/12,000 = 0.3780 = 37.80 cM
Incorrect D. ½×(954 + 959 + 984 + 4,152)/12,000 = ½×7,049/12,000 = 0.2937 = 29.37 cM
Incorrect E. ½×(3,936 + 4,152)/12,000 = ½×8,088/12,000 = 0.3370 = 33.70 cM
Incorrect F. ½×(954 + 959 + 984 + 1,015)/12,000 = ½×3,912/12,000 = 0.1630 = 16.30 cM
Correct MC

11bc_890a

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + d d d d
779 
+ + d d + + d d
119 
+ + d d d d + +
104 
d d + + + + d d
117 
d d + + d d + +
112 
d d d d + + + +
769 
TOTAL2,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene D and its centromere.

A. ½×(104 + 112 + 117 + 119)/2,000 = ½×452/2,000 = 0.1130 = 11.30 cM
Correct B. ½×(769 + 779)/2,000 = ½×1,548/2,000 = 0.3870 = 38.70 cM
Incorrect C. ½×(104 + 112 + 119 + 769)/2,000 = ½×1,104/2,000 = 0.2760 = 27.60 cM
Incorrect D. ½×(112 + 117 + 769 + 779)/2,000 = ½×1,777/2,000 = 0.4442 = 44.42 cM
Incorrect E. ½×(104 + 769)/2,000 = ½×873/2,000 = 0.2182 = 21.82 cM
Incorrect F. ½×(112 + 117)/2,000 = ½×229/2,000 = 0.0573 = 5.73 cM
Incorrect MC

b102_9cd3

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + p p p p
6,305 
+ + p p + + p p
2,610 
+ + p p p p + +
2,653 
p p + + + + p p
2,720 
p p + + p p + +
2,574 
p p p p + + + +
6,138 
TOTAL23,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene P and its centromere.

A. ½×(2,574 + 2,653 + 2,720 + 6,305)/23,000 = ½×14,252/23,000 = 0.3098 = 30.98 cM
Incorrect B. ½×(2,720 + 6,138)/23,000 = ½×8,858/23,000 = 0.1926 = 19.26 cM
Incorrect C. ½×(2,574 + 2,653 + 6,138 + 6,305)/23,000 = ½×17,670/23,000 = 0.3841 = 38.41 cM
Incorrect D. ½×(2,653 + 2,720 + 6,138)/23,000 = ½×11,511/23,000 = 0.2502 = 25.02 cM
Incorrect E. ½×(6,138 + 6,305)/23,000 = ½×12,443/23,000 = 0.2705 = 27.05 cM
Incorrect F. ½×(2,574 + 2,610 + 2,653 + 2,720)/23,000 = ½×10,557/23,000 = 0.2295 = 22.95 cM
Correct MC

097a_76a6

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + e e e e
1,889 
+ + e e + + e e
535 
+ + e e e e + +
515 
e e + + + + e e
510 
e e + + e e + +
546 
e e e e + + + +
2,005 
TOTAL6,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene E and its centromere.

A. ½×(510 + 546 + 1,889 + 2,005)/6,000 = ½×4,950/6,000 = 0.4125 = 41.25 cM
Incorrect B. ½×(510 + 535 + 2,005)/6,000 = ½×3,050/6,000 = 0.2542 = 25.42 cM
Incorrect C. ½×(535 + 546 + 2,005)/6,000 = ½×3,086/6,000 = 0.2572 = 25.72 cM
Incorrect D. ½×(1,889 + 2,005)/6,000 = ½×3,894/6,000 = 0.3245 = 32.45 cM
Incorrect E. ½×(510 + 515 + 535 + 546)/6,000 = ½×2,106/6,000 = 0.1755 = 17.55 cM
Correct F. ½×(546 + 1,889)/6,000 = ½×2,435/6,000 = 0.2029 = 20.29 cM
Incorrect MC

d8c8_e008

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + a a a a
1,830 
+ + a a + + a a
440 
+ + a a a a + +
414 
a a + + + + a a
433 
a a + + a a + +
468 
a a a a + + + +
1,815 
TOTAL5,400
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene A and its centromere.

A. ½×(1,815 + 1,830)/5,400 = ½×3,645/5,400 = 0.3375 = 33.75 cM
Incorrect B. ½×(414 + 440 + 1,815)/5,400 = ½×2,669/5,400 = 0.2471 = 24.71 cM
Incorrect C. ½×(414 + 433)/5,400 = ½×847/5,400 = 0.0784 = 7.84 cM
Incorrect D. ½×(440 + 468 + 1,830)/5,400 = ½×2,738/5,400 = 0.2535 = 25.35 cM
Incorrect E. ½×(414 + 468)/5,400 = ½×882/5,400 = 0.0817 = 8.17 cM
Incorrect F. ½×(414 + 433 + 440 + 468)/5,400 = ½×1,755/5,400 = 0.1625 = 16.25 cM
Correct MC

461e_b4d0

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
1,703 
+ + t t + + t t
710 
+ + t t t t + +
651 
t t + + + + t t
619 
t t + + t t + +
648 
t t t t + + + +
1,669 
TOTAL6,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(1,669 + 1,703)/6,000 = ½×3,372/6,000 = 0.2810 = 28.10 cM
Incorrect B. ½×(619 + 648 + 651 + 710)/6,000 = ½×2,628/6,000 = 0.2190 = 21.90 cM
Correct C. ½×(710 + 1,669)/6,000 = ½×2,379/6,000 = 0.1983 = 19.82 cM
Incorrect D. ½×(619 + 1,703)/6,000 = ½×2,322/6,000 = 0.1935 = 19.35 cM
Incorrect E. ½×(648 + 710 + 1,669)/6,000 = ½×3,027/6,000 = 0.2522 = 25.22 cM
Incorrect F. ½×(648 + 651 + 710)/6,000 = ½×2,009/6,000 = 0.1674 = 16.74 cM
Incorrect MC

408d_2ae4

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + p p p p
767 
+ + p p + + p p
351 
+ + p p p p + +
342 
p p + + + + p p
305 
p p + + p p + +
365 
p p p p + + + +
770 
TOTAL2,900
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene P and its centromere.

A. ½×(365 + 770)/2,900 = ½×1,135/2,900 = 0.1957 = 19.57 cM
Incorrect B. ½×(305 + 342 + 351 + 365)/2,900 = ½×1,363/2,900 = 0.2350 = 23.50 cM
Correct C. ½×(351 + 365 + 767)/2,900 = ½×1,483/2,900 = 0.2557 = 25.57 cM
Incorrect D. ½×(351 + 365 + 767 + 770)/2,900 = ½×2,253/2,900 = 0.3884 = 38.84 cM
Incorrect E. ½×(767 + 770)/2,900 = ½×1,537/2,900 = 0.2650 = 26.50 cM
Incorrect F. ½×(305 + 342 + 351)/2,900 = ½×998/2,900 = 0.1721 = 17.21 cM
Incorrect MC

1148_3d71

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + j j j j
2,236 
+ + j j + + j j
275 
+ + j j j j + +
318 
j j + + + + j j
295 
j j + + j j + +
316 
j j j j + + + +
2,160 
TOTAL5,600
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene J and its centromere.

A. ½×(275 + 295 + 316 + 318)/5,600 = ½×1,204/5,600 = 0.1075 = 10.75 cM
Correct B. ½×(275 + 316 + 2,160)/5,600 = ½×2,751/5,600 = 0.2456 = 24.56 cM
Incorrect C. ½×(2,160 + 2,236)/5,600 = ½×4,396/5,600 = 0.3925 = 39.25 cM
Incorrect D. ½×(275 + 318)/5,600 = ½×593/5,600 = 0.0529 = 5.29 cM
Incorrect E. ½×(318 + 2,160)/5,600 = ½×2,478/5,600 = 0.2213 = 22.12 cM
Incorrect F. ½×(275 + 295 + 2,236)/5,600 = ½×2,806/5,600 = 0.2505 = 25.05 cM
Incorrect MC

371c_ed3c

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + m m m m
5,407 
+ + m m + + m m
924 
+ + m m m m + +
902 
m m + + + + m m
947 
m m + + m m + +
939 
m m m m + + + +
5,381 
TOTAL14,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene M and its centromere.

A. ½×(902 + 939 + 5,407)/14,500 = ½×7,248/14,500 = 0.2499 = 24.99 cM
Incorrect B. ½×(902 + 924 + 939 + 947)/14,500 = ½×3,712/14,500 = 0.1280 = 12.80 cM
Correct C. ½×(939 + 947 + 5,381 + 5,407)/14,500 = ½×12,674/14,500 = 0.4370 = 43.70 cM
Incorrect D. ½×(5,381 + 5,407)/14,500 = ½×10,788/14,500 = 0.3720 = 37.20 cM
Incorrect E. ½×(939 + 5,381 + 5,407)/14,500 = ½×11,727/14,500 = 0.4044 = 40.44 cM
Incorrect F. ½×(939 + 5,381)/14,500 = ½×6,320/14,500 = 0.2179 = 21.79 cM
Incorrect MC

8a6e_1e8d

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
7,575 
+ + t t + + t t
3,231 
+ + t t t t + +
3,172 
t t + + + + t t
3,089 
t t + + t t + +
3,192 
t t t t + + + +
7,741 
TOTAL28,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(3,089 + 3,231 + 7,575)/28,000 = ½×13,895/28,000 = 0.2481 = 24.81 cM
Incorrect B. ½×(3,231 + 7,741)/28,000 = ½×10,972/28,000 = 0.1959 = 19.59 cM
Incorrect C. ½×(3,172 + 3,192 + 3,231 + 7,741)/28,000 = ½×17,336/28,000 = 0.3096 = 30.96 cM
Incorrect D. ½×(3,089 + 3,172 + 3,231 + 7,575)/28,000 = ½×17,067/28,000 = 0.3048 = 30.48 cM
Incorrect E. ½×(3,089 + 3,172 + 3,192 + 3,231)/28,000 = ½×12,684/28,000 = 0.2265 = 22.65 cM
Correct F. ½×(7,575 + 7,741)/28,000 = ½×15,316/28,000 = 0.2735 = 27.35 cM
Incorrect MC

6b27_6f97

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + x x x x
1,729 
+ + x x + + x x
1,002 
+ + x x x x + +
1,031 
x x + + + + x x
1,016 
x x + + x x + +
956 
x x x x + + + +
1,766 
TOTAL7,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene X and its centromere.

A. ½×(956 + 1,002 + 1,031 + 1,729)/7,500 = ½×4,718/7,500 = 0.3145 = 31.45 cM
Incorrect B. ½×(956 + 1,002 + 1,729 + 1,766)/7,500 = ½×5,453/7,500 = 0.3635 = 36.35 cM
Incorrect C. ½×(1,016 + 1,729 + 1,766)/7,500 = ½×4,511/7,500 = 0.3007 = 30.07 cM
Incorrect D. ½×(1,729 + 1,766)/7,500 = ½×3,495/7,500 = 0.2330 = 23.30 cM
Incorrect E. ½×(956 + 1,016)/7,500 = ½×1,972/7,500 = 0.1315 = 13.15 cM
Incorrect F. ½×(956 + 1,002 + 1,016 + 1,031)/7,500 = ½×4,005/7,500 = 0.2670 = 26.70 cM
Correct MC

15e1_5fba

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + k k k k
8,663 
+ + k k + + k k
2,725 
+ + k k k k + +
2,695 
k k + + + + k k
2,586 
k k + + k k + +
2,662 
k k k k + + + +
8,669 
TOTAL28,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene K and its centromere.

A. ½×(8,663 + 8,669)/28,000 = ½×17,332/28,000 = 0.3095 = 30.95 cM
Incorrect B. ½×(2,586 + 2,662 + 2,695 + 2,725)/28,000 = ½×10,668/28,000 = 0.1905 = 19.05 cM
Correct C. ½×(2,695 + 2,725 + 8,663)/28,000 = ½×14,083/28,000 = 0.2515 = 25.15 cM
Incorrect D. ½×(2,586 + 8,669)/28,000 = ½×11,255/28,000 = 0.2010 = 20.10 cM
Incorrect E. ½×(2,662 + 2,695 + 8,663 + 8,669)/28,000 = ½×22,689/28,000 = 0.4052 = 40.52 cM
Incorrect F. ½×(2,662 + 2,695)/28,000 = ½×5,357/28,000 = 0.0957 = 9.57 cM
Incorrect MC

2d32_354f

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
2,764 
+ + t t + + t t
953 
+ + t t t t + +
1,030 
t t + + + + t t
1,014 
t t + + t t + +
1,031 
t t t t + + + +
2,708 
TOTAL9,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(2,708 + 2,764)/9,500 = ½×5,472/9,500 = 0.2880 = 28.80 cM
Incorrect B. ½×(953 + 1,031)/9,500 = ½×1,984/9,500 = 0.1044 = 10.44 cM
Incorrect C. ½×(953 + 1,014 + 1,030 + 1,031)/9,500 = ½×4,028/9,500 = 0.2120 = 21.20 cM
Correct D. ½×(953 + 1,030 + 2,764)/9,500 = ½×4,747/9,500 = 0.2498 = 24.98 cM
Incorrect E. ½×(953 + 1,030 + 1,031 + 2,708)/9,500 = ½×5,722/9,500 = 0.3012 = 30.12 cM
Incorrect F. ½×(1,031 + 2,708 + 2,764)/9,500 = ½×6,503/9,500 = 0.3423 = 34.23 cM
Incorrect MC

0f09_44c3

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + c c c c
946 
+ + c c + + c c
290 
+ + c c c c + +
301 
c c + + + + c c
284 
c c + + c c + +
307 
c c c c + + + +
872 
TOTAL3,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene C and its centromere.

A. ½×(284 + 290 + 301 + 307)/3,000 = ½×1,182/3,000 = 0.1970 = 19.70 cM
Correct B. ½×(301 + 307 + 872)/3,000 = ½×1,480/3,000 = 0.2467 = 24.67 cM
Incorrect C. ½×(290 + 307 + 872 + 946)/3,000 = ½×2,415/3,000 = 0.4025 = 40.25 cM
Incorrect D. ½×(284 + 290)/3,000 = ½×574/3,000 = 0.0957 = 9.57 cM
Incorrect E. ½×(284 + 290 + 946)/3,000 = ½×1,520/3,000 = 0.2533 = 25.33 cM
Incorrect F. ½×(872 + 946)/3,000 = ½×1,818/3,000 = 0.3030 = 30.30 cM
Incorrect MC

4453_a92d

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + p p p p
4,023 
+ + p p + + p p
1,280 
+ + p p p p + +
1,212 
p p + + + + p p
1,302 
p p + + p p + +
1,302 
p p p p + + + +
3,881 
TOTAL13,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene P and its centromere.

A. ½×(1,212 + 1,302)/13,000 = ½×2,514/13,000 = 0.0967 = 9.67 cM
Incorrect B. ½×(1,212 + 1,280 + 1,302 + 1,302)/13,000 = ½×5,096/13,000 = 0.1960 = 19.60 cM
Correct C. ½×(3,881 + 4,023)/13,000 = ½×7,904/13,000 = 0.3040 = 30.40 cM
Incorrect D. ½×(1,212 + 4,023)/13,000 = ½×5,235/13,000 = 0.2013 = 20.13 cM
Incorrect E. ½×(1,280 + 3,881)/13,000 = ½×5,161/13,000 = 0.1985 = 19.85 cM
Incorrect F. ½×(1,212 + 1,302 + 1,302)/13,000 = ½×3,816/13,000 = 0.1468 = 14.68 cM
Incorrect MC

0465_53c6

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + y y y y
3,412 
+ + y y + + y y
657 
+ + y y y y + +
657 
y y + + + + y y
638 
y y + + y y + +
670 
y y y y + + + +
3,466 
TOTAL9,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene Y and its centromere.

A. ½×(3,412 + 3,466)/9,500 = ½×6,878/9,500 = 0.3620 = 36.20 cM
Incorrect B. ½×(638 + 657 + 657 + 3,412)/9,500 = ½×5,364/9,500 = 0.2823 = 28.23 cM
Incorrect C. ½×(638 + 657 + 657 + 670)/9,500 = ½×2,622/9,500 = 0.1380 = 13.80 cM
Correct D. ½×(657 + 657 + 3,412 + 3,466)/9,500 = ½×8,192/9,500 = 0.4312 = 43.12 cM
Incorrect E. ½×(638 + 670 + 3,412 + 3,466)/9,500 = ½×8,186/9,500 = 0.4308 = 43.08 cM
Incorrect F. ½×(638 + 657 + 3,412 + 3,466)/9,500 = ½×8,173/9,500 = 0.4302 = 43.02 cM
Incorrect MC

1007_5053

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + n n n n
4,561 
+ + n n + + n n
1,160 
+ + n n n n + +
1,259 
n n + + + + n n
1,114 
n n + + n n + +
1,241 
n n n n + + + +
4,665 
TOTAL14,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene N and its centromere.

A. ½×(1,114 + 1,259)/14,000 = ½×2,373/14,000 = 0.0848 = 8.48 cM
Incorrect B. ½×(1,259 + 4,561)/14,000 = ½×5,820/14,000 = 0.2079 = 20.79 cM
Incorrect C. ½×(4,561 + 4,665)/14,000 = ½×9,226/14,000 = 0.3295 = 32.95 cM
Incorrect D. ½×(1,114 + 1,160 + 1,241 + 1,259)/14,000 = ½×4,774/14,000 = 0.1705 = 17.05 cM
Correct E. ½×(1,241 + 4,561)/14,000 = ½×5,802/14,000 = 0.2072 = 20.72 cM
Incorrect F. ½×(1,114 + 1,241 + 4,561)/14,000 = ½×6,916/14,000 = 0.2470 = 24.70 cM
Incorrect MC

402c_02c2

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + w w w w
9,115 
+ + w w + + w w
1,913 
+ + w w w w + +
1,980 
w w + + + + w w
1,944 
w w + + w w + +
1,885 
w w w w + + + +
9,163 
TOTAL26,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene W and its centromere.

A. ½×(1,885 + 1,913 + 9,115)/26,000 = ½×12,913/26,000 = 0.2483 = 24.83 cM
Incorrect B. ½×(9,115 + 9,163)/26,000 = ½×18,278/26,000 = 0.3515 = 35.15 cM
Incorrect C. ½×(1,885 + 1,944)/26,000 = ½×3,829/26,000 = 0.0736 = 7.36 cM
Incorrect D. ½×(1,885 + 1,913 + 1,944 + 1,980)/26,000 = ½×7,722/26,000 = 0.1485 = 14.85 cM
Correct E. ½×(1,980 + 9,115)/26,000 = ½×11,095/26,000 = 0.2134 = 21.34 cM
Incorrect F. ½×(1,885 + 1,913 + 1,944)/26,000 = ½×5,742/26,000 = 0.1104 = 11.04 cM
Incorrect MC

ca0f_7afa

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + n n n n
4,243 
+ + n n + + n n
1,465 
+ + n n n n + +
1,500 
n n + + + + n n
1,460 
n n + + n n + +
1,549 
n n n n + + + +
4,283 
TOTAL14,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene N and its centromere.

A. ½×(1,460 + 1,465 + 1,500 + 1,549)/14,500 = ½×5,974/14,500 = 0.2060 = 20.60 cM
Correct B. ½×(1,460 + 1,465 + 1,549)/14,500 = ½×4,474/14,500 = 0.1543 = 15.43 cM
Incorrect C. ½×(4,243 + 4,283)/14,500 = ½×8,526/14,500 = 0.2940 = 29.40 cM
Incorrect D. ½×(1,465 + 1,500 + 1,549)/14,500 = ½×4,514/14,500 = 0.1557 = 15.57 cM
Incorrect E. ½×(1,465 + 4,283)/14,500 = ½×5,748/14,500 = 0.1982 = 19.82 cM
Incorrect F. ½×(1,460 + 1,465 + 4,243)/14,500 = ½×7,168/14,500 = 0.2472 = 24.72 cM
Incorrect MC

dce4_74f5

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + k k k k
519 
+ + k k + + k k
236 
+ + k k k k + +
229 
k k + + + + k k
238 
k k + + k k + +
249 
k k k k + + + +
529 
TOTAL2,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene K and its centromere.

A. ½×(236 + 249)/2,000 = ½×485/2,000 = 0.1212 = 12.12 cM
Incorrect B. ½×(249 + 519 + 529)/2,000 = ½×1,297/2,000 = 0.3242 = 32.42 cM
Incorrect C. ½×(236 + 238 + 519 + 529)/2,000 = ½×1,522/2,000 = 0.3805 = 38.05 cM
Incorrect D. ½×(519 + 529)/2,000 = ½×1,048/2,000 = 0.2620 = 26.20 cM
Incorrect E. ½×(229 + 236)/2,000 = ½×465/2,000 = 0.1163 = 11.62 cM
Incorrect F. ½×(229 + 236 + 238 + 249)/2,000 = ½×952/2,000 = 0.2380 = 23.80 cM
Correct MC

d8f0_4bd9

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + k k k k
2,984 
+ + k k + + k k
747 
+ + k k k k + +
762 
k k + + + + k k
785 
k k + + k k + +
757 
k k k k + + + +
2,965 
TOTAL9,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene K and its centromere.

A. ½×(757 + 785 + 2,965)/9,000 = ½×4,507/9,000 = 0.2504 = 25.04 cM
Incorrect B. ½×(747 + 757 + 762 + 785)/9,000 = ½×3,051/9,000 = 0.1695 = 16.95 cM
Correct C. ½×(2,965 + 2,984)/9,000 = ½×5,949/9,000 = 0.3305 = 33.05 cM
Incorrect D. ½×(757 + 785)/9,000 = ½×1,542/9,000 = 0.0857 = 8.57 cM
Incorrect E. ½×(757 + 762 + 2,965)/9,000 = ½×4,484/9,000 = 0.2491 = 24.91 cM
Incorrect F. ½×(747 + 757 + 762 + 2,984)/9,000 = ½×5,250/9,000 = 0.2917 = 29.17 cM
Incorrect MC

ee4a_e71c

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + f f f f
4,193 
+ + f f + + f f
1,385 
+ + f f f f + +
1,327 
f f + + + + f f
1,381 
f f + + f f + +
1,339 
f f f f + + + +
4,375 
TOTAL14,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene F and its centromere.

A. ½×(1,339 + 1,385 + 4,375)/14,000 = ½×7,099/14,000 = 0.2535 = 25.35 cM
Incorrect B. ½×(1,327 + 1,339 + 1,381 + 4,375)/14,000 = ½×8,422/14,000 = 0.3008 = 30.08 cM
Incorrect C. ½×(4,193 + 4,375)/14,000 = ½×8,568/14,000 = 0.3060 = 30.60 cM
Incorrect D. ½×(1,327 + 1,381 + 4,375)/14,000 = ½×7,083/14,000 = 0.2530 = 25.30 cM
Incorrect E. ½×(1,327 + 1,339 + 1,381 + 1,385)/14,000 = ½×5,432/14,000 = 0.1940 = 19.40 cM
Correct F. ½×(1,339 + 1,385 + 4,193)/14,000 = ½×6,917/14,000 = 0.2470 = 24.70 cM
Incorrect MC

5899_d2cf

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + h h h h
2,320 
+ + h h + + h h
1,319 
+ + h h h h + +
1,372 
h h + + + + h h
1,318 
h h + + h h + +
1,381 
h h h h + + + +
2,290 
TOTAL10,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene H and its centromere.

A. ½×(2,290 + 2,320)/10,000 = ½×4,610/10,000 = 0.2305 = 23.05 cM
Incorrect B. ½×(1,318 + 1,319 + 1,372 + 1,381)/10,000 = ½×5,390/10,000 = 0.2695 = 26.95 cM
Correct C. ½×(1,319 + 1,372 + 2,290 + 2,320)/10,000 = ½×7,301/10,000 = 0.3650 = 36.50 cM
Incorrect D. ½×(1,372 + 2,290 + 2,320)/10,000 = ½×5,982/10,000 = 0.2991 = 29.91 cM
Incorrect E. ½×(1,318 + 1,372 + 1,381)/10,000 = ½×4,071/10,000 = 0.2036 = 20.36 cM
Incorrect F. ½×(1,372 + 1,381 + 2,320)/10,000 = ½×5,073/10,000 = 0.2536 = 25.36 cM
Incorrect MC

8a8d_2b86

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + c c c c
3,618 
+ + c c + + c c
1,699 
+ + c c c c + +
1,632 
c c + + + + c c
1,618 
c c + + c c + +
1,743 
c c c c + + + +
3,690 
TOTAL14,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene C and its centromere.

A. ½×(3,618 + 3,690)/14,000 = ½×7,308/14,000 = 0.2610 = 26.10 cM
Incorrect B. ½×(1,618 + 1,632 + 1,699 + 1,743)/14,000 = ½×6,692/14,000 = 0.2390 = 23.90 cM
Correct C. ½×(1,618 + 1,699 + 1,743 + 3,618)/14,000 = ½×8,678/14,000 = 0.3099 = 30.99 cM
Incorrect D. ½×(1,618 + 1,632 + 1,743)/14,000 = ½×4,993/14,000 = 0.1783 = 17.83 cM
Incorrect E. ½×(1,618 + 3,618 + 3,690)/14,000 = ½×8,926/14,000 = 0.3188 = 31.88 cM
Incorrect F. ½×(1,632 + 1,743)/14,000 = ½×3,375/14,000 = 0.1205 = 12.05 cM
Incorrect MC

cc8f_b0d0

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + w w w w
9,762 
+ + w w + + w w
1,940 
+ + w w w w + +
1,851 
w w + + + + w w
1,884 
w w + + w w + +
1,858 
w w w w + + + +
9,705 
TOTAL27,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene W and its centromere.

A. ½×(9,705 + 9,762)/27,000 = ½×19,467/27,000 = 0.3605 = 36.05 cM
Incorrect B. ½×(1,940 + 9,705)/27,000 = ½×11,645/27,000 = 0.2156 = 21.56 cM
Incorrect C. ½×(1,851 + 1,858 + 1,884 + 1,940)/27,000 = ½×7,533/27,000 = 0.1395 = 13.95 cM
Correct D. ½×(1,884 + 9,705)/27,000 = ½×11,589/27,000 = 0.2146 = 21.46 cM
Incorrect E. ½×(1,858 + 1,940 + 9,705)/27,000 = ½×13,503/27,000 = 0.2501 = 25.01 cM
Incorrect F. ½×(1,851 + 1,858 + 9,705)/27,000 = ½×13,414/27,000 = 0.2484 = 24.84 cM
Incorrect MC

599c_00a5

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + c c c c
4,593 
+ + c c + + c c
1,173 
+ + c c c c + +
1,220 
c c + + + + c c
1,192 
c c + + c c + +
1,189 
c c c c + + + +
4,633 
TOTAL14,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene C and its centromere.

A. ½×(1,173 + 1,192 + 4,633)/14,000 = ½×6,998/14,000 = 0.2499 = 24.99 cM
Incorrect B. ½×(4,593 + 4,633)/14,000 = ½×9,226/14,000 = 0.3295 = 32.95 cM
Incorrect C. ½×(1,173 + 1,192 + 4,593 + 4,633)/14,000 = ½×11,591/14,000 = 0.4140 = 41.40 cM
Incorrect D. ½×(1,173 + 1,189 + 1,192 + 1,220)/14,000 = ½×4,774/14,000 = 0.1705 = 17.05 cM
Correct E. ½×(1,173 + 1,189 + 1,192 + 4,593)/14,000 = ½×8,147/14,000 = 0.2910 = 29.10 cM
Incorrect F. ½×(1,189 + 1,192 + 1,220 + 4,633)/14,000 = ½×8,234/14,000 = 0.2941 = 29.41 cM
Incorrect MC

03c7_52ab

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + c c c c
5,187 
+ + c c + + c c
570 
+ + c c c c + +
593 
c c + + + + c c
628 
c c + + c c + +
601 
c c c c + + + +
5,421 
TOTAL13,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene C and its centromere.

A. ½×(570 + 593 + 601 + 628)/13,000 = ½×2,392/13,000 = 0.0920 = 9.20 cM
Correct B. ½×(5,187 + 5,421)/13,000 = ½×10,608/13,000 = 0.4080 = 40.80 cM
Incorrect C. ½×(593 + 5,421)/13,000 = ½×6,014/13,000 = 0.2313 = 23.13 cM
Incorrect D. ½×(570 + 5,421)/13,000 = ½×5,991/13,000 = 0.2304 = 23.04 cM
Incorrect E. ½×(593 + 601 + 5,187 + 5,421)/13,000 = ½×11,802/13,000 = 0.4539 = 45.39 cM
Incorrect F. ½×(593 + 601 + 628 + 5,421)/13,000 = ½×7,243/13,000 = 0.2786 = 27.86 cM
Incorrect MC

16d9_e14c

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + e e e e
409 
+ + e e + + e e
309 
+ + e e e e + +
239 
e e + + + + e e
256 
e e + + e e + +
279 
e e e e + + + +
408 
TOTAL1,900
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene E and its centromere.

A. ½×(408 + 409)/1,900 = ½×817/1,900 = 0.2150 = 21.50 cM
Incorrect B. ½×(256 + 279 + 408 + 409)/1,900 = ½×1,352/1,900 = 0.3558 = 35.58 cM
Incorrect C. ½×(239 + 279 + 409)/1,900 = ½×927/1,900 = 0.2439 = 24.39 cM
Incorrect D. ½×(239 + 256 + 279 + 309)/1,900 = ½×1,083/1,900 = 0.2850 = 28.50 cM
Correct E. ½×(239 + 256 + 309 + 409)/1,900 = ½×1,213/1,900 = 0.3192 = 31.92 cM
Incorrect F. ½×(279 + 408 + 409)/1,900 = ½×1,096/1,900 = 0.2884 = 28.84 cM
Incorrect MC

6bd4_17bc

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + b b b b
2,676 
+ + b b + + b b
1,635 
+ + b b b b + +
1,708 
b b + + + + b b
1,662 
b b + + b b + +
1,643 
b b b b + + + +
2,676 
TOTAL12,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene B and its centromere.

A. ½×(1,635 + 1,643 + 1,662 + 1,708)/12,000 = ½×6,648/12,000 = 0.2770 = 27.70 cM
Correct B. ½×(2,676 + 2,676)/12,000 = ½×5,352/12,000 = 0.2230 = 22.30 cM
Incorrect C. ½×(1,635 + 1,708)/12,000 = ½×3,343/12,000 = 0.1393 = 13.93 cM
Incorrect D. ½×(1,643 + 2,676)/12,000 = ½×4,319/12,000 = 0.1800 = 18.00 cM
Incorrect E. ½×(1,635 + 1,643 + 1,708 + 2,676)/12,000 = ½×7,662/12,000 = 0.3192 = 31.92 cM
Incorrect F. ½×(1,635 + 1,643)/12,000 = ½×3,278/12,000 = 0.1366 = 13.66 cM
Incorrect MC

56ed_cb3c

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + j j j j
2,083 
+ + j j + + j j
463 
+ + j j j j + +
446 
j j + + + + j j
460 
j j + + j j + +
455 
j j j j + + + +
2,093 
TOTAL6,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene J and its centromere.

A. ½×(446 + 455 + 460 + 463)/6,000 = ½×1,824/6,000 = 0.1520 = 15.20 cM
Correct B. ½×(455 + 460 + 2,083)/6,000 = ½×2,998/6,000 = 0.2498 = 24.98 cM
Incorrect C. ½×(446 + 460 + 463 + 2,083)/6,000 = ½×3,452/6,000 = 0.2877 = 28.77 cM
Incorrect D. ½×(460 + 2,083 + 2,093)/6,000 = ½×4,636/6,000 = 0.3863 = 38.63 cM
Incorrect E. ½×(446 + 455 + 460)/6,000 = ½×1,361/6,000 = 0.1134 = 11.34 cM
Incorrect F. ½×(2,083 + 2,093)/6,000 = ½×4,176/6,000 = 0.3480 = 34.80 cM
Incorrect MC

ab6e_46d1

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + y y y y
270 
+ + y y + + y y
64 
+ + y y y y + +
60 
y y + + + + y y
60 
y y + + y y + +
64 
y y y y + + + +
282 
TOTAL800
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene Y and its centromere.

A. ½×(270 + 282)/800 = ½×552/800 = 0.3450 = 34.50 cM
Incorrect B. ½×(60 + 60 + 64 + 64)/800 = ½×248/800 = 0.1550 = 15.50 cM
Correct C. ½×(60 + 64 + 270)/800 = ½×394/800 = 0.2462 = 24.62 cM
Incorrect D. ½×(60 + 64 + 282)/800 = ½×406/800 = 0.2537 = 25.37 cM
Incorrect E. ½×(64 + 64 + 270 + 282)/800 = ½×680/800 = 0.4250 = 42.50 cM
Incorrect F. ½×(60 + 64)/800 = ½×124/800 = 0.0775 = 7.75 cM
Incorrect MC

7f09_50a4

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + m m m m
1,047 
+ + m m + + m m
440 
+ + m m m m + +
480 
m m + + + + m m
487 
m m + + m m + +
549 
m m m m + + + +
997 
TOTAL4,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene M and its centromere.

A. ½×(440 + 549 + 997 + 1,047)/4,000 = ½×3,033/4,000 = 0.3791 = 37.91 cM
Incorrect B. ½×(440 + 487 + 549 + 1,047)/4,000 = ½×2,523/4,000 = 0.3154 = 31.54 cM
Incorrect C. ½×(440 + 487 + 1,047)/4,000 = ½×1,974/4,000 = 0.2467 = 24.68 cM
Incorrect D. ½×(440 + 487 + 997 + 1,047)/4,000 = ½×2,971/4,000 = 0.3714 = 37.14 cM
Incorrect E. ½×(440 + 480 + 487 + 549)/4,000 = ½×1,956/4,000 = 0.2445 = 24.45 cM
Correct F. ½×(997 + 1,047)/4,000 = ½×2,044/4,000 = 0.2555 = 25.55 cM
Incorrect MC

2c50_a7c4

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + r r r r
2,128 
+ + r r + + r r
1,196 
+ + r r r r + +
1,164 
r r + + + + r r
1,189 
r r + + r r + +
1,167 
r r r r + + + +
2,156 
TOTAL9,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene R and its centromere.

A. ½×(1,164 + 1,167 + 1,189 + 1,196)/9,000 = ½×4,716/9,000 = 0.2620 = 26.20 cM
Correct B. ½×(1,164 + 1,167 + 1,189 + 2,128)/9,000 = ½×5,648/9,000 = 0.3138 = 31.38 cM
Incorrect C. ½×(1,189 + 1,196 + 2,156)/9,000 = ½×4,541/9,000 = 0.2523 = 25.23 cM
Incorrect D. ½×(1,196 + 2,156)/9,000 = ½×3,352/9,000 = 0.1862 = 18.62 cM
Incorrect E. ½×(1,164 + 1,167 + 1,189 + 2,156)/9,000 = ½×5,676/9,000 = 0.3153 = 31.53 cM
Incorrect F. ½×(2,128 + 2,156)/9,000 = ½×4,284/9,000 = 0.2380 = 23.80 cM
Incorrect MC

3a0d_6f64

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
1,062 
+ + t t + + t t
546 
+ + t t t t + +
589 
t t + + + + t t
597 
t t + + t t + +
590 
t t t t + + + +
1,116 
TOTAL4,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(546 + 589 + 590 + 597)/4,500 = ½×2,322/4,500 = 0.2580 = 25.80 cM
Correct B. ½×(1,062 + 1,116)/4,500 = ½×2,178/4,500 = 0.2420 = 24.20 cM
Incorrect C. ½×(589 + 1,062 + 1,116)/4,500 = ½×2,767/4,500 = 0.3074 = 30.74 cM
Incorrect D. ½×(590 + 1,062)/4,500 = ½×1,652/4,500 = 0.1836 = 18.36 cM
Incorrect E. ½×(546 + 597 + 1,062)/4,500 = ½×2,205/4,500 = 0.2450 = 24.50 cM
Incorrect F. ½×(546 + 590 + 1,062 + 1,116)/4,500 = ½×3,314/4,500 = 0.3682 = 36.82 cM
Incorrect MC

94e0_b82b

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + r r r r
5,429 
+ + r r + + r r
714 
+ + r r r r + +
723 
r r + + + + r r
682 
r r + + r r + +
689 
r r r r + + + +
5,263 
TOTAL13,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene R and its centromere.

A. ½×(682 + 689 + 714 + 723)/13,500 = ½×2,808/13,500 = 0.1040 = 10.40 cM
Correct B. ½×(682 + 5,429)/13,500 = ½×6,111/13,500 = 0.2263 = 22.63 cM
Incorrect C. ½×(682 + 714 + 723 + 5,429)/13,500 = ½×7,548/13,500 = 0.2796 = 27.96 cM
Incorrect D. ½×(689 + 5,263 + 5,429)/13,500 = ½×11,381/13,500 = 0.4215 = 42.15 cM
Incorrect E. ½×(5,263 + 5,429)/13,500 = ½×10,692/13,500 = 0.3960 = 39.60 cM
Incorrect F. ½×(714 + 5,429)/13,500 = ½×6,143/13,500 = 0.2275 = 22.75 cM
Incorrect MC

dfae_9c98

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + d d d d
2,262 
+ + d d + + d d
1,339 
+ + d d d d + +
1,376 
d d + + + + d d
1,360 
d d + + d d + +
1,415 
d d d d + + + +
2,248 
TOTAL10,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene D and its centromere.

A. ½×(1,415 + 2,248 + 2,262)/10,000 = ½×5,925/10,000 = 0.2963 = 29.62 cM
Incorrect B. ½×(1,339 + 1,360 + 1,376 + 2,262)/10,000 = ½×6,337/10,000 = 0.3169 = 31.69 cM
Incorrect C. ½×(1,339 + 1,415 + 2,248)/10,000 = ½×5,002/10,000 = 0.2501 = 25.01 cM
Incorrect D. ½×(1,339 + 1,360 + 1,376 + 1,415)/10,000 = ½×5,490/10,000 = 0.2745 = 27.45 cM
Correct E. ½×(1,376 + 1,415 + 2,248 + 2,262)/10,000 = ½×7,301/10,000 = 0.3650 = 36.50 cM
Incorrect F. ½×(2,248 + 2,262)/10,000 = ½×4,510/10,000 = 0.2255 = 22.55 cM
Incorrect MC

0ca6_7da3

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
9,767 
+ + t t + + t t
1,109 
+ + t t t t + +
1,101 
t t + + + + t t
1,116 
t t + + t t + +
1,066 
t t t t + + + +
9,841 
TOTAL24,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(1,066 + 1,101)/24,000 = ½×2,167/24,000 = 0.0451 = 4.51 cM
Incorrect B. ½×(1,066 + 1,101 + 9,841)/24,000 = ½×12,008/24,000 = 0.2502 = 25.02 cM
Incorrect C. ½×(9,767 + 9,841)/24,000 = ½×19,608/24,000 = 0.4085 = 40.85 cM
Incorrect D. ½×(1,066 + 1,101 + 1,109 + 1,116)/24,000 = ½×4,392/24,000 = 0.0915 = 9.15 cM
Correct E. ½×(1,066 + 1,101 + 1,116 + 9,841)/24,000 = ½×13,124/24,000 = 0.2734 = 27.34 cM
Incorrect F. ½×(1,066 + 1,101 + 9,767 + 9,841)/24,000 = ½×21,775/24,000 = 0.4536 = 45.36 cM
Incorrect MC

b331_69f9

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + r r r r
512 
+ + r r + + r r
171 
+ + r r r r + +
177 
r r + + + + r r
156 
r r + + r r + +
176 
r r r r + + + +
508 
TOTAL1,700
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene R and its centromere.

A. ½×(176 + 508 + 512)/1,700 = ½×1,196/1,700 = 0.3518 = 35.18 cM
Incorrect B. ½×(508 + 512)/1,700 = ½×1,020/1,700 = 0.3000 = 30.00 cM
Incorrect C. ½×(171 + 176)/1,700 = ½×347/1,700 = 0.1021 = 10.21 cM
Incorrect D. ½×(171 + 176 + 177 + 508)/1,700 = ½×1,032/1,700 = 0.3035 = 30.35 cM
Incorrect E. ½×(156 + 512)/1,700 = ½×668/1,700 = 0.1965 = 19.65 cM
Incorrect F. ½×(156 + 171 + 176 + 177)/1,700 = ½×680/1,700 = 0.2000 = 20.00 cM
Correct MC

485a_29e4

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + p p p p
914 
+ + p p + + p p
394 
+ + p p p p + +
454 
p p + + + + p p
418 
p p + + p p + +
407 
p p p p + + + +
913 
TOTAL3,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene P and its centromere.

A. ½×(394 + 407 + 913)/3,500 = ½×1,714/3,500 = 0.2449 = 24.49 cM
Incorrect B. ½×(418 + 914)/3,500 = ½×1,332/3,500 = 0.1903 = 19.03 cM
Incorrect C. ½×(913 + 914)/3,500 = ½×1,827/3,500 = 0.2610 = 26.10 cM
Incorrect D. ½×(407 + 914)/3,500 = ½×1,321/3,500 = 0.1887 = 18.87 cM
Incorrect E. ½×(407 + 454)/3,500 = ½×861/3,500 = 0.1230 = 12.30 cM
Incorrect F. ½×(394 + 407 + 418 + 454)/3,500 = ½×1,673/3,500 = 0.2390 = 23.90 cM
Correct MC

9302_b0bf

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + r r r r
239 
+ + r r + + r r
59 
+ + r r r r + +
50 
r r + + + + r r
60 
r r + + r r + +
62 
r r r r + + + +
230 
TOTAL700
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene R and its centromere.

A. ½×(50 + 62 + 239)/700 = ½×351/700 = 0.2507 = 25.07 cM
Incorrect B. ½×(50 + 59 + 239)/700 = ½×348/700 = 0.2486 = 24.86 cM
Incorrect C. ½×(50 + 60 + 62)/700 = ½×172/700 = 0.1229 = 12.29 cM
Incorrect D. ½×(50 + 59 + 60 + 62)/700 = ½×231/700 = 0.1650 = 16.50 cM
Correct E. ½×(50 + 62 + 230 + 239)/700 = ½×581/700 = 0.4150 = 41.50 cM
Incorrect F. ½×(230 + 239)/700 = ½×469/700 = 0.3350 = 33.50 cM
Incorrect MC

e2fa_5ed3

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + c c c c
2,951 
+ + c c + + c c
1,056 
+ + c c c c + +
1,008 
c c + + + + c c
1,092 
c c + + c c + +
1,074 
c c c c + + + +
2,819 
TOTAL10,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene C and its centromere.

A. ½×(1,008 + 1,056 + 2,819 + 2,951)/10,000 = ½×7,834/10,000 = 0.3917 = 39.17 cM
Incorrect B. ½×(1,008 + 1,074 + 1,092 + 2,819)/10,000 = ½×5,993/10,000 = 0.2997 = 29.97 cM
Incorrect C. ½×(1,056 + 1,074 + 1,092 + 2,951)/10,000 = ½×6,173/10,000 = 0.3086 = 30.86 cM
Incorrect D. ½×(1,008 + 1,056 + 1,074 + 1,092)/10,000 = ½×4,230/10,000 = 0.2115 = 21.15 cM
Correct E. ½×(2,819 + 2,951)/10,000 = ½×5,770/10,000 = 0.2885 = 28.85 cM
Incorrect F. ½×(1,074 + 1,092)/10,000 = ½×2,166/10,000 = 0.1083 = 10.83 cM
Incorrect MC

6596_bc48

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + f f f f
3,983 
+ + f f + + f f
1,162 
+ + f f f f + +
1,111 
f f + + + + f f
1,157 
f f + + f f + +
1,145 
f f f f + + + +
3,942 
TOTAL12,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene F and its centromere.

A. ½×(1,157 + 1,162)/12,500 = ½×2,319/12,500 = 0.0928 = 9.28 cM
Incorrect B. ½×(1,111 + 1,145 + 1,157 + 1,162)/12,500 = ½×4,575/12,500 = 0.1830 = 18.30 cM
Correct C. ½×(1,111 + 1,145 + 1,162)/12,500 = ½×3,418/12,500 = 0.1367 = 13.67 cM
Incorrect D. ½×(3,942 + 3,983)/12,500 = ½×7,925/12,500 = 0.3170 = 31.70 cM
Incorrect E. ½×(1,145 + 1,157 + 1,162 + 3,983)/12,500 = ½×7,447/12,500 = 0.2979 = 29.79 cM
Incorrect F. ½×(1,111 + 1,157)/12,500 = ½×2,268/12,500 = 0.0907 = 9.07 cM
Incorrect MC

454f_072c

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + c c c c
1,521 
+ + c c + + c c
622 
+ + c c c c + +
619 
c c + + + + c c
615 
c c + + c c + +
597 
c c c c + + + +
1,526 
TOTAL5,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene C and its centromere.

A. ½×(1,521 + 1,526)/5,500 = ½×3,047/5,500 = 0.2770 = 27.70 cM
Incorrect B. ½×(597 + 619 + 1,526)/5,500 = ½×2,742/5,500 = 0.2493 = 24.93 cM
Incorrect C. ½×(597 + 615 + 619 + 622)/5,500 = ½×2,453/5,500 = 0.2230 = 22.30 cM
Correct D. ½×(619 + 1,521)/5,500 = ½×2,140/5,500 = 0.1945 = 19.45 cM
Incorrect E. ½×(597 + 615 + 619 + 1,526)/5,500 = ½×3,357/5,500 = 0.3052 = 30.52 cM
Incorrect F. ½×(597 + 619 + 1,521 + 1,526)/5,500 = ½×4,263/5,500 = 0.3875 = 38.75 cM
Incorrect MC

35b3_3112

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + b b b b
563 
+ + b b + + b b
113 
+ + b b b b + +
96 
b b + + + + b b
101 
b b + + b b + +
107 
b b b b + + + +
520 
TOTAL1,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene B and its centromere.

A. ½×(96 + 101 + 113 + 520)/1,500 = ½×830/1,500 = 0.2767 = 27.67 cM
Incorrect B. ½×(107 + 563)/1,500 = ½×670/1,500 = 0.2233 = 22.33 cM
Incorrect C. ½×(96 + 101 + 107 + 113)/1,500 = ½×417/1,500 = 0.1390 = 13.90 cM
Correct D. ½×(520 + 563)/1,500 = ½×1,083/1,500 = 0.3610 = 36.10 cM
Incorrect E. ½×(96 + 101 + 113)/1,500 = ½×310/1,500 = 0.1033 = 10.33 cM
Incorrect F. ½×(101 + 113 + 520)/1,500 = ½×734/1,500 = 0.2447 = 24.47 cM
Incorrect MC

4450_d4ff

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + x x x x
505 
+ + x x + + x x
236 
+ + x x x x + +
252 
x x + + + + x x
246 
x x + + x x + +
236 
x x x x + + + +
525 
TOTAL2,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene X and its centromere.

A. ½×(505 + 525)/2,000 = ½×1,030/2,000 = 0.2575 = 25.75 cM
Incorrect B. ½×(236 + 236 + 525)/2,000 = ½×997/2,000 = 0.2492 = 24.93 cM
Incorrect C. ½×(236 + 252 + 505 + 525)/2,000 = ½×1,518/2,000 = 0.3795 = 37.95 cM
Incorrect D. ½×(236 + 246 + 252 + 505)/2,000 = ½×1,239/2,000 = 0.3098 = 30.98 cM
Incorrect E. ½×(236 + 252)/2,000 = ½×488/2,000 = 0.1220 = 12.20 cM
Incorrect F. ½×(236 + 236 + 246 + 252)/2,000 = ½×970/2,000 = 0.2425 = 24.25 cM
Correct MC

e1ab_e109

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + k k k k
810 
+ + k k + + k k
102 
+ + k k k k + +
103 
k k + + + + k k
88 
k k + + k k + +
91 
k k k k + + + +
806 
TOTAL2,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene K and its centromere.

A. ½×(88 + 91 + 102 + 103)/2,000 = ½×384/2,000 = 0.0960 = 9.60 cM
Correct B. ½×(103 + 806 + 810)/2,000 = ½×1,719/2,000 = 0.4298 = 42.98 cM
Incorrect C. ½×(88 + 102 + 810)/2,000 = ½×1,000/2,000 = 0.2500 = 25.00 cM
Incorrect D. ½×(806 + 810)/2,000 = ½×1,616/2,000 = 0.4040 = 40.40 cM
Incorrect E. ½×(88 + 103 + 806 + 810)/2,000 = ½×1,807/2,000 = 0.4517 = 45.17 cM
Incorrect F. ½×(88 + 91)/2,000 = ½×179/2,000 = 0.0447 = 4.47 cM
Incorrect MC

a516_d40e

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + k k k k
3,877 
+ + k k + + k k
458 
+ + k k k k + +
431 
k k + + + + k k
439 
k k + + k k + +
439 
k k k k + + + +
3,856 
TOTAL9,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene K and its centromere.

A. ½×(431 + 439)/9,500 = ½×870/9,500 = 0.0458 = 4.58 cM
Incorrect B. ½×(431 + 439 + 439 + 458)/9,500 = ½×1,767/9,500 = 0.0930 = 9.30 cM
Correct C. ½×(3,856 + 3,877)/9,500 = ½×7,733/9,500 = 0.4070 = 40.70 cM
Incorrect D. ½×(439 + 458)/9,500 = ½×897/9,500 = 0.0472 = 4.72 cM
Incorrect E. ½×(439 + 439)/9,500 = ½×878/9,500 = 0.0462 = 4.62 cM
Incorrect F. ½×(431 + 439 + 3,877)/9,500 = ½×4,747/9,500 = 0.2498 = 24.98 cM
Incorrect MC

2129_2dc7

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + y y y y
194 
+ + y y + + y y
76 
+ + y y y y + +
90 
y y + + + + y y
67 
y y + + y y + +
89 
y y y y + + + +
184 
TOTAL700
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene Y and its centromere.

A. ½×(89 + 90)/700 = ½×179/700 = 0.1279 = 12.79 cM
Incorrect B. ½×(67 + 89 + 194)/700 = ½×350/700 = 0.2500 = 25.00 cM
Incorrect C. ½×(67 + 76 + 89 + 90)/700 = ½×322/700 = 0.2300 = 23.00 cM
Correct D. ½×(67 + 76 + 89 + 184)/700 = ½×416/700 = 0.2971 = 29.71 cM
Incorrect E. ½×(90 + 194)/700 = ½×284/700 = 0.2029 = 20.29 cM
Incorrect F. ½×(184 + 194)/700 = ½×378/700 = 0.2700 = 27.00 cM
Incorrect MC

2854_a546

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + c c c c
2,398 
+ + c c + + c c
1,126 
+ + c c c c + +
1,094 
c c + + + + c c
1,053 
c c + + c c + +
1,074 
c c c c + + + +
2,255 
TOTAL9,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene C and its centromere.

A. ½×(1,074 + 1,126)/9,000 = ½×2,200/9,000 = 0.1222 = 12.22 cM
Incorrect B. ½×(1,053 + 1,094 + 1,126 + 2,255)/9,000 = ½×5,528/9,000 = 0.3071 = 30.71 cM
Incorrect C. ½×(1,053 + 1,074 + 1,094 + 1,126)/9,000 = ½×4,347/9,000 = 0.2415 = 24.15 cM
Correct D. ½×(1,094 + 1,126 + 2,255)/9,000 = ½×4,475/9,000 = 0.2486 = 24.86 cM
Incorrect E. ½×(1,053 + 1,074 + 2,255 + 2,398)/9,000 = ½×6,780/9,000 = 0.3767 = 37.67 cM
Incorrect F. ½×(2,255 + 2,398)/9,000 = ½×4,653/9,000 = 0.2585 = 25.85 cM
Incorrect MC

5464_9daa

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + w w w w
446 
+ + w w + + w w
57 
+ + w w w w + +
62 
w w + + + + w w
81 
w w + + w w + +
58 
w w w w + + + +
496 
TOTAL1,200
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene W and its centromere.

A. ½×(57 + 58 + 62 + 81)/1,200 = ½×258/1,200 = 0.1075 = 10.75 cM
Correct B. ½×(62 + 81 + 446)/1,200 = ½×589/1,200 = 0.2454 = 24.54 cM
Incorrect C. ½×(446 + 496)/1,200 = ½×942/1,200 = 0.3925 = 39.25 cM
Incorrect D. ½×(57 + 58)/1,200 = ½×115/1,200 = 0.0479 = 4.79 cM
Incorrect E. ½×(58 + 81 + 446)/1,200 = ½×585/1,200 = 0.2437 = 24.38 cM
Incorrect F. ½×(58 + 446 + 496)/1,200 = ½×1,000/1,200 = 0.4167 = 41.67 cM
Incorrect MC

4837_e7dd

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + a a a a
151 
+ + a a + + a a
65 
+ + a a a a + +
85 
a a + + + + a a
82 
a a + + a a + +
71 
a a a a + + + +
146 
TOTAL600
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene A and its centromere.

A. ½×(65 + 71 + 146)/600 = ½×282/600 = 0.2350 = 23.50 cM
Incorrect B. ½×(82 + 146 + 151)/600 = ½×379/600 = 0.3158 = 31.58 cM
Incorrect C. ½×(146 + 151)/600 = ½×297/600 = 0.2475 = 24.75 cM
Incorrect D. ½×(65 + 71 + 82 + 85)/600 = ½×303/600 = 0.2525 = 25.25 cM
Correct E. ½×(65 + 82 + 85 + 146)/600 = ½×378/600 = 0.3150 = 31.50 cM
Incorrect F. ½×(65 + 71 + 146 + 151)/600 = ½×433/600 = 0.3608 = 36.08 cM
Incorrect MC

917d_1629

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + a a a a
2,335 
+ + a a + + a a
1,344 
+ + a a a a + +
1,336 
a a + + + + a a
1,277 
a a + + a a + +
1,423 
a a a a + + + +
2,285 
TOTAL10,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene A and its centromere.

A. ½×(1,277 + 1,344 + 2,285)/10,000 = ½×4,906/10,000 = 0.2453 = 24.53 cM
Incorrect B. ½×(1,277 + 1,336 + 1,423 + 2,285)/10,000 = ½×6,321/10,000 = 0.3160 = 31.61 cM
Incorrect C. ½×(1,336 + 1,423)/10,000 = ½×2,759/10,000 = 0.1379 = 13.79 cM
Incorrect D. ½×(2,285 + 2,335)/10,000 = ½×4,620/10,000 = 0.2310 = 23.10 cM
Incorrect E. ½×(1,277 + 1,423 + 2,285 + 2,335)/10,000 = ½×7,320/10,000 = 0.3660 = 36.60 cM
Incorrect F. ½×(1,277 + 1,336 + 1,344 + 1,423)/10,000 = ½×5,380/10,000 = 0.2690 = 26.90 cM
Correct MC

1ef9_4ed7

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + a a a a
2,652 
+ + a a + + a a
787 
+ + a a a a + +
802 
a a + + + + a a
781 
a a + + a a + +
826 
a a a a + + + +
2,652 
TOTAL8,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene A and its centromere.

A. ½×(781 + 802 + 826 + 2,652)/8,500 = ½×5,061/8,500 = 0.2977 = 29.77 cM
Incorrect B. ½×(781 + 802 + 2,652 + 2,652)/8,500 = ½×6,887/8,500 = 0.4051 = 40.51 cM
Incorrect C. ½×(2,652 + 2,652)/8,500 = ½×5,304/8,500 = 0.3120 = 31.20 cM
Incorrect D. ½×(787 + 826 + 2,652 + 2,652)/8,500 = ½×6,917/8,500 = 0.4069 = 40.69 cM
Incorrect E. ½×(781 + 802 + 826)/8,500 = ½×2,409/8,500 = 0.1417 = 14.17 cM
Incorrect F. ½×(781 + 787 + 802 + 826)/8,500 = ½×3,196/8,500 = 0.1880 = 18.80 cM
Correct MC

f01a_76be

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + k k k k
486 
+ + k k + + k k
279 
+ + k k k k + +
221 
k k + + + + k k
278 
k k + + k k + +
272 
k k k k + + + +
464 
TOTAL2,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene K and its centromere.

A. ½×(464 + 486)/2,000 = ½×950/2,000 = 0.2375 = 23.75 cM
Incorrect B. ½×(279 + 486)/2,000 = ½×765/2,000 = 0.1913 = 19.12 cM
Incorrect C. ½×(221 + 272 + 278 + 279)/2,000 = ½×1,050/2,000 = 0.2625 = 26.25 cM
Correct D. ½×(279 + 464)/2,000 = ½×743/2,000 = 0.1857 = 18.57 cM
Incorrect E. ½×(278 + 486)/2,000 = ½×764/2,000 = 0.1910 = 19.10 cM
Incorrect F. ½×(221 + 272 + 464 + 486)/2,000 = ½×1,443/2,000 = 0.3608 = 36.08 cM
Incorrect MC

2d6c_d861

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + x x x x
3,541 
+ + x x + + x x
1,473 
+ + x x x x + +
1,485 
x x + + + + x x
1,436 
x x + + x x + +
1,482 
x x x x + + + +
3,583 
TOTAL13,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene X and its centromere.

A. ½×(1,473 + 1,485 + 3,541 + 3,583)/13,000 = ½×10,082/13,000 = 0.3878 = 38.78 cM
Incorrect B. ½×(1,436 + 1,485 + 3,541 + 3,583)/13,000 = ½×10,045/13,000 = 0.3863 = 38.63 cM
Incorrect C. ½×(3,541 + 3,583)/13,000 = ½×7,124/13,000 = 0.2740 = 27.40 cM
Incorrect D. ½×(1,436 + 1,473 + 1,482 + 1,485)/13,000 = ½×5,876/13,000 = 0.2260 = 22.60 cM
Correct E. ½×(1,482 + 3,541)/13,000 = ½×5,023/13,000 = 0.1932 = 19.32 cM
Incorrect F. ½×(1,473 + 1,482)/13,000 = ½×2,955/13,000 = 0.1137 = 11.37 cM
Incorrect MC

0e7c_a8dc

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + d d d d
1,195 
+ + d d + + d d
525 
+ + d d d d + +
554 
d d + + + + d d
586 
d d + + d d + +
513 
d d d d + + + +
1,127 
TOTAL4,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene D and its centromere.

A. ½×(586 + 1,195)/4,500 = ½×1,781/4,500 = 0.1979 = 19.79 cM
Incorrect B. ½×(513 + 1,127 + 1,195)/4,500 = ½×2,835/4,500 = 0.3150 = 31.50 cM
Incorrect C. ½×(513 + 586 + 1,195)/4,500 = ½×2,294/4,500 = 0.2549 = 25.49 cM
Incorrect D. ½×(513 + 525 + 554 + 586)/4,500 = ½×2,178/4,500 = 0.2420 = 24.20 cM
Correct E. ½×(1,127 + 1,195)/4,500 = ½×2,322/4,500 = 0.2580 = 25.80 cM
Incorrect F. ½×(513 + 525 + 586 + 1,127)/4,500 = ½×2,751/4,500 = 0.3057 = 30.57 cM
Incorrect MC

6794_c99e

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
2,134 
+ + t t + + t t
886 
+ + t t t t + +
1,012 
t t + + + + t t
908 
t t + + t t + +
898 
t t t t + + + +
2,162 
TOTAL8,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(886 + 898 + 908 + 2,162)/8,000 = ½×4,854/8,000 = 0.3034 = 30.34 cM
Incorrect B. ½×(898 + 908 + 1,012 + 2,162)/8,000 = ½×4,980/8,000 = 0.3113 = 31.13 cM
Incorrect C. ½×(886 + 1,012 + 2,134 + 2,162)/8,000 = ½×6,194/8,000 = 0.3871 = 38.71 cM
Incorrect D. ½×(2,134 + 2,162)/8,000 = ½×4,296/8,000 = 0.2685 = 26.85 cM
Incorrect E. ½×(886 + 898 + 908 + 1,012)/8,000 = ½×3,704/8,000 = 0.2315 = 23.15 cM
Correct F. ½×(886 + 898)/8,000 = ½×1,784/8,000 = 0.1115 = 11.15 cM
Incorrect MC

680c_da68

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + d d d d
3,744 
+ + d d + + d d
480 
+ + d d d d + +
508 
d d + + + + d d
526 
d d + + d d + +
538 
d d d d + + + +
3,704 
TOTAL9,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene D and its centromere.

A. ½×(480 + 508)/9,500 = ½×988/9,500 = 0.0520 = 5.20 cM
Incorrect B. ½×(480 + 508 + 3,704)/9,500 = ½×4,692/9,500 = 0.2469 = 24.69 cM
Incorrect C. ½×(3,704 + 3,744)/9,500 = ½×7,448/9,500 = 0.3920 = 39.20 cM
Incorrect D. ½×(508 + 526 + 538)/9,500 = ½×1,572/9,500 = 0.0827 = 8.27 cM
Incorrect E. ½×(480 + 508 + 526 + 538)/9,500 = ½×2,052/9,500 = 0.1080 = 10.80 cM
Correct F. ½×(526 + 3,704 + 3,744)/9,500 = ½×7,974/9,500 = 0.4197 = 41.97 cM
Incorrect MC

9ca6_e4d3

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
863 
+ + t t + + t t
305 
+ + t t t t + +
298 
t t + + + + t t
331 
t t + + t t + +
314 
t t t t + + + +
889 
TOTAL3,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(305 + 331)/3,000 = ½×636/3,000 = 0.1060 = 10.60 cM
Incorrect B. ½×(298 + 314 + 863)/3,000 = ½×1,475/3,000 = 0.2458 = 24.58 cM
Incorrect C. ½×(298 + 863)/3,000 = ½×1,161/3,000 = 0.1935 = 19.35 cM
Incorrect D. ½×(863 + 889)/3,000 = ½×1,752/3,000 = 0.2920 = 29.20 cM
Incorrect E. ½×(298 + 314 + 331)/3,000 = ½×943/3,000 = 0.1572 = 15.72 cM
Incorrect F. ½×(298 + 305 + 314 + 331)/3,000 = ½×1,248/3,000 = 0.2080 = 20.80 cM
Correct MC

b64b_bcd9

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + j j j j
3,416 
+ + j j + + j j
1,137 
+ + j j j j + +
1,126 
j j + + + + j j
1,148 
j j + + j j + +
1,120 
j j j j + + + +
3,553 
TOTAL11,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene J and its centromere.

A. ½×(1,120 + 1,126 + 1,137 + 1,148)/11,500 = ½×4,531/11,500 = 0.1970 = 19.70 cM
Correct B. ½×(3,416 + 3,553)/11,500 = ½×6,969/11,500 = 0.3030 = 30.30 cM
Incorrect C. ½×(1,120 + 3,553)/11,500 = ½×4,673/11,500 = 0.2032 = 20.32 cM
Incorrect D. ½×(1,120 + 1,137 + 3,416)/11,500 = ½×5,673/11,500 = 0.2467 = 24.67 cM
Incorrect E. ½×(1,126 + 1,148)/11,500 = ½×2,274/11,500 = 0.0989 = 9.89 cM
Incorrect F. ½×(1,120 + 1,126 + 1,148 + 3,553)/11,500 = ½×6,947/11,500 = 0.3020 = 30.20 cM
Incorrect MC

1b05_5867

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + m m m m
7,323 
+ + m m + + m m
2,956 
+ + m m m m + +
3,159 
m m + + + + m m
3,136 
m m + + m m + +
3,088 
m m m m + + + +
7,338 
TOTAL27,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene M and its centromere.

A. ½×(2,956 + 3,088 + 3,136 + 3,159)/27,000 = ½×12,339/27,000 = 0.2285 = 22.85 cM
Correct B. ½×(2,956 + 3,136 + 7,323 + 7,338)/27,000 = ½×20,753/27,000 = 0.3843 = 38.43 cM
Incorrect C. ½×(2,956 + 7,323 + 7,338)/27,000 = ½×17,617/27,000 = 0.3262 = 32.62 cM
Incorrect D. ½×(2,956 + 3,159)/27,000 = ½×6,115/27,000 = 0.1132 = 11.32 cM
Incorrect E. ½×(3,136 + 7,338)/27,000 = ½×10,474/27,000 = 0.1940 = 19.40 cM
Incorrect F. ½×(7,323 + 7,338)/27,000 = ½×14,661/27,000 = 0.2715 = 27.15 cM
Incorrect MC

1aef_3a7d

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
8,032 
+ + t t + + t t
2,426 
+ + t t t t + +
2,459 
t t + + + + t t
2,433 
t t + + t t + +
2,484 
t t t t + + + +
8,166 
TOTAL26,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(8,032 + 8,166)/26,000 = ½×16,198/26,000 = 0.3115 = 31.15 cM
Incorrect B. ½×(2,426 + 2,433 + 2,459 + 2,484)/26,000 = ½×9,802/26,000 = 0.1885 = 18.85 cM
Correct C. ½×(2,426 + 2,459 + 8,032)/26,000 = ½×12,917/26,000 = 0.2484 = 24.84 cM
Incorrect D. ½×(2,426 + 2,484 + 8,032 + 8,166)/26,000 = ½×21,108/26,000 = 0.4059 = 40.59 cM
Incorrect E. ½×(2,433 + 8,032 + 8,166)/26,000 = ½×18,631/26,000 = 0.3583 = 35.83 cM
Incorrect F. ½×(2,426 + 2,484 + 8,032)/26,000 = ½×12,942/26,000 = 0.2489 = 24.89 cM
Incorrect MC

ba21_f7fd

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + d d d d
4,074 
+ + d d + + d d
2,751 
+ + d d d d + +
2,741 
d d + + + + d d
2,665 
d d + + d d + +
2,806 
d d d d + + + +
3,963 
TOTAL19,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene D and its centromere.

A. ½×(2,665 + 2,741 + 2,806)/19,000 = ½×8,212/19,000 = 0.2161 = 21.61 cM
Incorrect B. ½×(2,665 + 2,741 + 4,074)/19,000 = ½×9,480/19,000 = 0.2495 = 24.95 cM
Incorrect C. ½×(2,741 + 2,806 + 4,074)/19,000 = ½×9,621/19,000 = 0.2532 = 25.32 cM
Incorrect D. ½×(3,963 + 4,074)/19,000 = ½×8,037/19,000 = 0.2115 = 21.15 cM
Incorrect E. ½×(2,751 + 2,806 + 3,963 + 4,074)/19,000 = ½×13,594/19,000 = 0.3577 = 35.77 cM
Incorrect F. ½×(2,665 + 2,741 + 2,751 + 2,806)/19,000 = ½×10,963/19,000 = 0.2885 = 28.85 cM
Correct MC

e57c_f77b

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + b b b b
4,948 
+ + b b + + b b
2,931 
+ + b b b b + +
2,986 
b b + + + + b b
3,014 
b b + + b b + +
3,015 
b b b b + + + +
5,106 
TOTAL22,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene B and its centromere.

A. ½×(4,948 + 5,106)/22,000 = ½×10,054/22,000 = 0.2285 = 22.85 cM
Incorrect B. ½×(2,931 + 3,014)/22,000 = ½×5,945/22,000 = 0.1351 = 13.51 cM
Incorrect C. ½×(2,986 + 3,014 + 4,948)/22,000 = ½×10,948/22,000 = 0.2488 = 24.88 cM
Incorrect D. ½×(2,931 + 2,986 + 3,014 + 3,015)/22,000 = ½×11,946/22,000 = 0.2715 = 27.15 cM
Correct E. ½×(2,986 + 3,015)/22,000 = ½×6,001/22,000 = 0.1364 = 13.64 cM
Incorrect F. ½×(2,931 + 3,014 + 3,015 + 5,106)/22,000 = ½×14,066/22,000 = 0.3197 = 31.97 cM
Incorrect MC

b4a9_2ce3

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + r r r r
1,597 
+ + r r + + r r
454 
+ + r r r r + +
476 
r r + + + + r r
479 
r r + + r r + +
466 
r r r r + + + +
1,528 
TOTAL5,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene R and its centromere.

A. ½×(466 + 476 + 479 + 1,597)/5,000 = ½×3,018/5,000 = 0.3018 = 30.18 cM
Incorrect B. ½×(454 + 466 + 476 + 479)/5,000 = ½×1,875/5,000 = 0.1875 = 18.75 cM
Correct C. ½×(1,528 + 1,597)/5,000 = ½×3,125/5,000 = 0.3125 = 31.25 cM
Incorrect D. ½×(466 + 476 + 1,528)/5,000 = ½×2,470/5,000 = 0.2470 = 24.70 cM
Incorrect E. ½×(454 + 476)/5,000 = ½×930/5,000 = 0.0930 = 9.30 cM
Incorrect F. ½×(454 + 466 + 1,528)/5,000 = ½×2,448/5,000 = 0.2448 = 24.48 cM
Incorrect MC

9473_4a58

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + p p p p
676 
+ + p p + + p p
321 
+ + p p p p + +
321 
p p + + + + p p
310 
p p + + p p + +
309 
p p p p + + + +
663 
TOTAL2,600
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene P and its centromere.

A. ½×(663 + 676)/2,600 = ½×1,339/2,600 = 0.2575 = 25.75 cM
Incorrect B. ½×(310 + 321 + 663)/2,600 = ½×1,294/2,600 = 0.2488 = 24.88 cM
Incorrect C. ½×(309 + 321 + 676)/2,600 = ½×1,306/2,600 = 0.2512 = 25.12 cM
Incorrect D. ½×(309 + 310 + 321 + 321)/2,600 = ½×1,261/2,600 = 0.2425 = 24.25 cM
Correct E. ½×(309 + 310 + 676)/2,600 = ½×1,295/2,600 = 0.2490 = 24.90 cM
Incorrect F. ½×(309 + 676)/2,600 = ½×985/2,600 = 0.1894 = 18.94 cM
Incorrect MC

d0a6_f6a0

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + h h h h
886 
+ + h h + + h h
438 
+ + h h h h + +
460 
h h + + + + h h
411 
h h + + h h + +
399 
h h h h + + + +
906 
TOTAL3,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene H and its centromere.

A. ½×(411 + 438 + 886 + 906)/3,500 = ½×2,641/3,500 = 0.3773 = 37.73 cM
Incorrect B. ½×(399 + 906)/3,500 = ½×1,305/3,500 = 0.1864 = 18.64 cM
Incorrect C. ½×(399 + 411 + 438 + 460)/3,500 = ½×1,708/3,500 = 0.2440 = 24.40 cM
Correct D. ½×(399 + 411 + 886)/3,500 = ½×1,696/3,500 = 0.2423 = 24.23 cM
Incorrect E. ½×(399 + 460 + 886)/3,500 = ½×1,745/3,500 = 0.2493 = 24.93 cM
Incorrect F. ½×(886 + 906)/3,500 = ½×1,792/3,500 = 0.2560 = 25.60 cM
Incorrect MC

d212_5058

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + f f f f
8,947 
+ + f f + + f f
2,382 
+ + f f f f + +
2,246 
f f + + + + f f
2,313 
f f + + f f + +
2,266 
f f f f + + + +
8,846 
TOTAL27,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene F and its centromere.

A. ½×(8,846 + 8,947)/27,000 = ½×17,793/27,000 = 0.3295 = 32.95 cM
Incorrect B. ½×(2,246 + 2,266 + 2,313 + 2,382)/27,000 = ½×9,207/27,000 = 0.1705 = 17.05 cM
Correct C. ½×(2,246 + 2,266 + 2,382)/27,000 = ½×6,894/27,000 = 0.1277 = 12.77 cM
Incorrect D. ½×(2,246 + 2,266 + 2,382 + 8,846)/27,000 = ½×15,740/27,000 = 0.2915 = 29.15 cM
Incorrect E. ½×(2,266 + 8,846 + 8,947)/27,000 = ½×20,059/27,000 = 0.3715 = 37.15 cM
Incorrect F. ½×(2,246 + 2,313 + 8,846 + 8,947)/27,000 = ½×22,352/27,000 = 0.4139 = 41.39 cM
Incorrect MC

3883_191d

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + p p p p
78 
+ + p p + + p p
40 
+ + p p p p + +
28 
p p + + + + p p
32 
p p + + p p + +
41 
p p p p + + + +
81 
TOTAL300
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene P and its centromere.

A. ½×(28 + 32 + 40 + 81)/300 = ½×181/300 = 0.3017 = 30.17 cM
Incorrect B. ½×(78 + 81)/300 = ½×159/300 = 0.2650 = 26.50 cM
Incorrect C. ½×(40 + 41 + 78)/300 = ½×159/300 = 0.2650 = 26.50 cM
Incorrect D. ½×(28 + 32 + 40 + 41)/300 = ½×141/300 = 0.2350 = 23.50 cM
Correct E. ½×(32 + 40 + 41 + 81)/300 = ½×194/300 = 0.3233 = 32.33 cM
Incorrect F. ½×(32 + 41 + 81)/300 = ½×154/300 = 0.2567 = 25.67 cM
Incorrect MC

da21_d413

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + r r r r
5,011 
+ + r r + + r r
543 
+ + r r r r + +
615 
r r + + + + r r
572 
r r + + r r + +
570 
r r r r + + + +
5,189 
TOTAL12,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene R and its centromere.

A. ½×(572 + 615 + 5,011)/12,500 = ½×6,198/12,500 = 0.2479 = 24.79 cM
Incorrect B. ½×(543 + 5,189)/12,500 = ½×5,732/12,500 = 0.2293 = 22.93 cM
Incorrect C. ½×(543 + 570 + 5,011 + 5,189)/12,500 = ½×11,313/12,500 = 0.4525 = 45.25 cM
Incorrect D. ½×(615 + 5,011)/12,500 = ½×5,626/12,500 = 0.2250 = 22.50 cM
Incorrect E. ½×(5,011 + 5,189)/12,500 = ½×10,200/12,500 = 0.4080 = 40.80 cM
Incorrect F. ½×(543 + 570 + 572 + 615)/12,500 = ½×2,300/12,500 = 0.0920 = 9.20 cM
Correct MC

ca7a_ec27

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + y y y y
10,927 
+ + y y + + y y
1,356 
+ + y y y y + +
1,273 
y y + + + + y y
1,263 
y y + + y y + +
1,319 
y y y y + + + +
10,862 
TOTAL27,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene Y and its centromere.

A. ½×(1,263 + 10,862 + 10,927)/27,000 = ½×23,052/27,000 = 0.4269 = 42.69 cM
Incorrect B. ½×(1,263 + 1,273 + 1,319 + 1,356)/27,000 = ½×5,211/27,000 = 0.0965 = 9.65 cM
Correct C. ½×(1,319 + 1,356)/27,000 = ½×2,675/27,000 = 0.0495 = 4.95 cM
Incorrect D. ½×(1,273 + 1,319)/27,000 = ½×2,592/27,000 = 0.0480 = 4.80 cM
Incorrect E. ½×(10,862 + 10,927)/27,000 = ½×21,789/27,000 = 0.4035 = 40.35 cM
Incorrect F. ½×(1,273 + 10,862 + 10,927)/27,000 = ½×23,062/27,000 = 0.4271 = 42.71 cM
Incorrect MC

edbe_dff2

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + c c c c
816 
+ + c c + + c c
155 
+ + c c c c + +
178 
c c + + + + c c
175 
c c + + c c + +
182 
c c c c + + + +
794 
TOTAL2,300
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene C and its centromere.

A. ½×(155 + 175 + 178 + 182)/2,300 = ½×690/2,300 = 0.1500 = 15.00 cM
Correct B. ½×(178 + 816)/2,300 = ½×994/2,300 = 0.2161 = 21.61 cM
Incorrect C. ½×(155 + 794 + 816)/2,300 = ½×1,765/2,300 = 0.3837 = 38.37 cM
Incorrect D. ½×(178 + 794)/2,300 = ½×972/2,300 = 0.2113 = 21.13 cM
Incorrect E. ½×(182 + 794)/2,300 = ½×976/2,300 = 0.2122 = 21.22 cM
Incorrect F. ½×(794 + 816)/2,300 = ½×1,610/2,300 = 0.3500 = 35.00 cM
Incorrect MC

3137_f40f

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + f f f f
328 
+ + f f + + f f
37 
+ + f f f f + +
41 
f f + + + + f f
33 
f f + + f f + +
37 
f f f f + + + +
324 
TOTAL800
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene F and its centromere.

A. ½×(33 + 37)/800 = ½×70/800 = 0.0437 = 4.38 cM
Incorrect B. ½×(33 + 37 + 37)/800 = ½×107/800 = 0.0669 = 6.69 cM
Incorrect C. ½×(37 + 41)/800 = ½×78/800 = 0.0488 = 4.88 cM
Incorrect D. ½×(37 + 37 + 41 + 324)/800 = ½×439/800 = 0.2744 = 27.44 cM
Incorrect E. ½×(33 + 37 + 37 + 41)/800 = ½×148/800 = 0.0925 = 9.25 cM
Correct F. ½×(324 + 328)/800 = ½×652/800 = 0.4075 = 40.75 cM
Incorrect MC

8fb0_3456

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + a a a a
1,488 
+ + a a + + a a
387 
+ + a a a a + +
381 
a a + + + + a a
374 
a a + + a a + +
352 
a a a a + + + +
1,518 
TOTAL4,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene A and its centromere.

A. ½×(352 + 374 + 381 + 1,488)/4,500 = ½×2,595/4,500 = 0.2883 = 28.83 cM
Incorrect B. ½×(352 + 374 + 1,518)/4,500 = ½×2,244/4,500 = 0.2493 = 24.93 cM
Incorrect C. ½×(374 + 387 + 1,488)/4,500 = ½×2,249/4,500 = 0.2499 = 24.99 cM
Incorrect D. ½×(374 + 387 + 1,488 + 1,518)/4,500 = ½×3,767/4,500 = 0.4186 = 41.86 cM
Incorrect E. ½×(352 + 374 + 381 + 387)/4,500 = ½×1,494/4,500 = 0.1660 = 16.60 cM
Correct F. ½×(1,488 + 1,518)/4,500 = ½×3,006/4,500 = 0.3340 = 33.40 cM
Incorrect MC

a16e_2c18

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + j j j j
1,890 
+ + j j + + j j
1,054 
+ + j j j j + +
1,068 
j j + + + + j j
1,015 
j j + + j j + +
1,047 
j j j j + + + +
1,926 
TOTAL8,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene J and its centromere.

A. ½×(1,015 + 1,047 + 1,054 + 1,890)/8,000 = ½×5,006/8,000 = 0.3129 = 31.29 cM
Incorrect B. ½×(1,054 + 1,068)/8,000 = ½×2,122/8,000 = 0.1326 = 13.26 cM
Incorrect C. ½×(1,015 + 1,068)/8,000 = ½×2,083/8,000 = 0.1302 = 13.02 cM
Incorrect D. ½×(1,890 + 1,926)/8,000 = ½×3,816/8,000 = 0.2385 = 23.85 cM
Incorrect E. ½×(1,054 + 1,068 + 1,926)/8,000 = ½×4,048/8,000 = 0.2530 = 25.30 cM
Incorrect F. ½×(1,015 + 1,047 + 1,054 + 1,068)/8,000 = ½×4,184/8,000 = 0.2615 = 26.15 cM
Correct MC

8894_ef6a

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + a a a a
2,954 
+ + a a + + a a
1,760 
+ + a a a a + +
1,789 
a a + + + + a a
1,742 
a a + + a a + +
1,768 
a a a a + + + +
2,987 
TOTAL13,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene A and its centromere.

A. ½×(1,742 + 1,789 + 2,954)/13,000 = ½×6,485/13,000 = 0.2494 = 24.94 cM
Incorrect B. ½×(1,768 + 1,789)/13,000 = ½×3,557/13,000 = 0.1368 = 13.68 cM
Incorrect C. ½×(2,954 + 2,987)/13,000 = ½×5,941/13,000 = 0.2285 = 22.85 cM
Incorrect D. ½×(1,742 + 1,760 + 1,768 + 1,789)/13,000 = ½×7,059/13,000 = 0.2715 = 27.15 cM
Correct E. ½×(1,742 + 1,768 + 2,954 + 2,987)/13,000 = ½×9,451/13,000 = 0.3635 = 36.35 cM
Incorrect F. ½×(1,768 + 1,789 + 2,954)/13,000 = ½×6,511/13,000 = 0.2504 = 25.04 cM
Incorrect MC

95af_7187

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + y y y y
5,779 
+ + y y + + y y
877 
+ + y y y y + +
856 
y y + + + + y y
866 
y y + + y y + +
836 
y y y y + + + +
5,786 
TOTAL15,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene Y and its centromere.

A. ½×(836 + 866 + 877)/15,000 = ½×2,579/15,000 = 0.0860 = 8.60 cM
Incorrect B. ½×(5,779 + 5,786)/15,000 = ½×11,565/15,000 = 0.3855 = 38.55 cM
Incorrect C. ½×(877 + 5,779 + 5,786)/15,000 = ½×12,442/15,000 = 0.4147 = 41.47 cM
Incorrect D. ½×(836 + 856 + 866 + 877)/15,000 = ½×3,435/15,000 = 0.1145 = 11.45 cM
Correct E. ½×(856 + 866)/15,000 = ½×1,722/15,000 = 0.0574 = 5.74 cM
Incorrect F. ½×(836 + 856 + 866 + 5,786)/15,000 = ½×8,344/15,000 = 0.2781 = 27.81 cM
Incorrect MC

6bf0_3397

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + j j j j
8,254 
+ + j j + + j j
2,370 
+ + j j j j + +
2,426 
j j + + + + j j
2,315 
j j + + j j + +
2,275 
j j j j + + + +
8,360 
TOTAL26,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene J and its centromere.

A. ½×(2,275 + 2,315 + 2,370 + 2,426)/26,000 = ½×9,386/26,000 = 0.1805 = 18.05 cM
Correct B. ½×(2,426 + 8,360)/26,000 = ½×10,786/26,000 = 0.2074 = 20.74 cM
Incorrect C. ½×(8,254 + 8,360)/26,000 = ½×16,614/26,000 = 0.3195 = 31.95 cM
Incorrect D. ½×(2,275 + 2,315 + 2,426 + 8,254)/26,000 = ½×15,270/26,000 = 0.2937 = 29.37 cM
Incorrect E. ½×(2,315 + 8,360)/26,000 = ½×10,675/26,000 = 0.2053 = 20.53 cM
Incorrect F. ½×(2,275 + 2,370 + 8,254 + 8,360)/26,000 = ½×21,259/26,000 = 0.4088 = 40.88 cM
Incorrect MC

36c7_4e71

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + d d d d
608 
+ + d d + + d d
194 
+ + d d d d + +
179 
d d + + + + d d
209 
d d + + d d + +
202 
d d d d + + + +
608 
TOTAL2,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene D and its centromere.

A. ½×(179 + 194 + 202 + 209)/2,000 = ½×784/2,000 = 0.1960 = 19.60 cM
Correct B. ½×(179 + 209 + 608)/2,000 = ½×996/2,000 = 0.2490 = 24.90 cM
Incorrect C. ½×(194 + 608)/2,000 = ½×802/2,000 = 0.2005 = 20.05 cM
Incorrect D. ½×(202 + 608)/2,000 = ½×810/2,000 = 0.2025 = 20.25 cM
Incorrect E. ½×(202 + 209 + 608)/2,000 = ½×1,019/2,000 = 0.2547 = 25.47 cM
Incorrect F. ½×(608 + 608)/2,000 = ½×1,216/2,000 = 0.3040 = 30.40 cM
Incorrect MC

d339_88fe

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + f f f f
1,480 
+ + f f + + f f
309 
+ + f f f f + +
306 
f f + + + + f f
287 
f f + + f f + +
337 
f f f f + + + +
1,481 
TOTAL4,200
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene F and its centromere.

A. ½×(1,480 + 1,481)/4,200 = ½×2,961/4,200 = 0.3525 = 35.25 cM
Incorrect B. ½×(287 + 337 + 1,480)/4,200 = ½×2,104/4,200 = 0.2505 = 25.05 cM
Incorrect C. ½×(287 + 1,481)/4,200 = ½×1,768/4,200 = 0.2105 = 21.05 cM
Incorrect D. ½×(337 + 1,481)/4,200 = ½×1,818/4,200 = 0.2164 = 21.64 cM
Incorrect E. ½×(287 + 306 + 309 + 337)/4,200 = ½×1,239/4,200 = 0.1475 = 14.75 cM
Correct F. ½×(287 + 309 + 337 + 1,480)/4,200 = ½×2,413/4,200 = 0.2873 = 28.73 cM
Incorrect MC

4650_b9b3

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + f f f f
613 
+ + f f + + f f
223 
+ + f f f f + +
204 
f f + + + + f f
241 
f f + + f f + +
235 
f f f f + + + +
584 
TOTAL2,100
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene F and its centromere.

A. ½×(584 + 613)/2,100 = ½×1,197/2,100 = 0.2850 = 28.50 cM
Incorrect B. ½×(223 + 235 + 241)/2,100 = ½×699/2,100 = 0.1664 = 16.64 cM
Incorrect C. ½×(223 + 235 + 241 + 584)/2,100 = ½×1,283/2,100 = 0.3055 = 30.55 cM
Incorrect D. ½×(204 + 223 + 235 + 241)/2,100 = ½×903/2,100 = 0.2150 = 21.50 cM
Correct E. ½×(204 + 235)/2,100 = ½×439/2,100 = 0.1045 = 10.45 cM
Incorrect F. ½×(204 + 223 + 613)/2,100 = ½×1,040/2,100 = 0.2476 = 24.76 cM
Incorrect MC

ef6e_9b0a

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + d d d d
3,910 
+ + d d + + d d
533 
+ + d d d d + +
513 
d d + + + + d d
562 
d d + + d d + +
572 
d d d d + + + +
3,910 
TOTAL10,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene D and its centromere.

A. ½×(513 + 562)/10,000 = ½×1,075/10,000 = 0.0537 = 5.38 cM
Incorrect B. ½×(533 + 562 + 3,910 + 3,910)/10,000 = ½×8,915/10,000 = 0.4457 = 44.57 cM
Incorrect C. ½×(513 + 533 + 562 + 572)/10,000 = ½×2,180/10,000 = 0.1090 = 10.90 cM
Correct D. ½×(3,910 + 3,910)/10,000 = ½×7,820/10,000 = 0.3910 = 39.10 cM
Incorrect E. ½×(533 + 572)/10,000 = ½×1,105/10,000 = 0.0553 = 5.53 cM
Incorrect F. ½×(562 + 572 + 3,910)/10,000 = ½×5,044/10,000 = 0.2522 = 25.22 cM
Incorrect MC

b3ad_a277

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + x x x x
2,259 
+ + x x + + x x
615 
+ + x x x x + +
596 
x x + + + + x x
615 
x x + + x x + +
673 
x x x x + + + +
2,242 
TOTAL7,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene X and its centromere.

A. ½×(596 + 673 + 2,242 + 2,259)/7,000 = ½×5,770/7,000 = 0.4121 = 41.21 cM
Incorrect B. ½×(596 + 673 + 2,259)/7,000 = ½×3,528/7,000 = 0.2520 = 25.20 cM
Incorrect C. ½×(596 + 615 + 673 + 2,259)/7,000 = ½×4,143/7,000 = 0.2959 = 29.59 cM
Incorrect D. ½×(596 + 615 + 615 + 673)/7,000 = ½×2,499/7,000 = 0.1785 = 17.85 cM
Correct E. ½×(596 + 615 + 673)/7,000 = ½×1,884/7,000 = 0.1346 = 13.46 cM
Incorrect F. ½×(2,242 + 2,259)/7,000 = ½×4,501/7,000 = 0.3215 = 32.15 cM
Incorrect MC

19a7_a855

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
2,137 
+ + t t + + t t
897 
+ + t t t t + +
906 
t t + + + + t t
923 
t t + + t t + +
922 
t t t t + + + +
2,215 
TOTAL8,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(897 + 906 + 922 + 923)/8,000 = ½×3,648/8,000 = 0.2280 = 22.80 cM
Correct B. ½×(897 + 906)/8,000 = ½×1,803/8,000 = 0.1127 = 11.27 cM
Incorrect C. ½×(897 + 2,215)/8,000 = ½×3,112/8,000 = 0.1945 = 19.45 cM
Incorrect D. ½×(906 + 923 + 2,137)/8,000 = ½×3,966/8,000 = 0.2479 = 24.79 cM
Incorrect E. ½×(2,137 + 2,215)/8,000 = ½×4,352/8,000 = 0.2720 = 27.20 cM
Incorrect F. ½×(897 + 2,137)/8,000 = ½×3,034/8,000 = 0.1896 = 18.96 cM
Incorrect MC

ca09_823e

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + j j j j
10,902 
+ + j j + + j j
1,769 
+ + j j j j + +
1,856 
j j + + + + j j
1,757 
j j + + j j + +
1,839 
j j j j + + + +
10,877 
TOTAL29,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene J and its centromere.

A. ½×(1,856 + 10,877)/29,000 = ½×12,733/29,000 = 0.2195 = 21.95 cM
Incorrect B. ½×(1,769 + 1,839 + 1,856)/29,000 = ½×5,464/29,000 = 0.0942 = 9.42 cM
Incorrect C. ½×(1,757 + 1,856 + 10,877)/29,000 = ½×14,490/29,000 = 0.2498 = 24.98 cM
Incorrect D. ½×(10,877 + 10,902)/29,000 = ½×21,779/29,000 = 0.3755 = 37.55 cM
Incorrect E. ½×(1,769 + 10,877 + 10,902)/29,000 = ½×23,548/29,000 = 0.4060 = 40.60 cM
Incorrect F. ½×(1,757 + 1,769 + 1,839 + 1,856)/29,000 = ½×7,221/29,000 = 0.1245 = 12.45 cM
Correct MC

9450_9dde

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + k k k k
143 
+ + k k + + k k
38 
+ + k k k k + +
32 
k k + + + + k k
34 
k k + + k k + +
36 
k k k k + + + +
117 
TOTAL400
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene K and its centromere.

A. ½×(38 + 143)/400 = ½×181/400 = 0.2263 = 22.62 cM
Incorrect B. ½×(32 + 34 + 36)/400 = ½×102/400 = 0.1275 = 12.75 cM
Incorrect C. ½×(117 + 143)/400 = ½×260/400 = 0.3250 = 32.50 cM
Incorrect D. ½×(36 + 38 + 143)/400 = ½×217/400 = 0.2712 = 27.12 cM
Incorrect E. ½×(32 + 34 + 36 + 38)/400 = ½×140/400 = 0.1750 = 17.50 cM
Correct F. ½×(32 + 34 + 117 + 143)/400 = ½×326/400 = 0.4075 = 40.75 cM
Incorrect MC

3ba6_29a9

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + r r r r
3,023 
+ + r r + + r r
1,982 
+ + r r r r + +
2,026 
r r + + + + r r
1,952 
r r + + r r + +
1,992 
r r r r + + + +
3,025 
TOTAL14,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene R and its centromere.

A. ½×(1,952 + 1,982 + 1,992 + 2,026)/14,000 = ½×7,952/14,000 = 0.2840 = 28.40 cM
Correct B. ½×(1,982 + 2,026 + 3,023 + 3,025)/14,000 = ½×10,056/14,000 = 0.3591 = 35.91 cM
Incorrect C. ½×(1,952 + 1,992 + 2,026 + 3,025)/14,000 = ½×8,995/14,000 = 0.3212 = 32.12 cM
Incorrect D. ½×(3,023 + 3,025)/14,000 = ½×6,048/14,000 = 0.2160 = 21.60 cM
Incorrect E. ½×(1,982 + 1,992 + 3,023 + 3,025)/14,000 = ½×10,022/14,000 = 0.3579 = 35.79 cM
Incorrect F. ½×(1,982 + 1,992 + 2,026 + 3,025)/14,000 = ½×9,025/14,000 = 0.3223 = 32.23 cM
Incorrect MC

2ca1_2a9f

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
2,297 
+ + t t + + t t
617 
+ + t t t t + +
607 
t t + + + + t t
641 
t t + + t t + +
564 
t t t t + + + +
2,274 
TOTAL7,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(617 + 2,274 + 2,297)/7,000 = ½×5,188/7,000 = 0.3706 = 37.06 cM
Incorrect B. ½×(564 + 617 + 2,274 + 2,297)/7,000 = ½×5,752/7,000 = 0.4109 = 41.09 cM
Incorrect C. ½×(564 + 607 + 617 + 641)/7,000 = ½×2,429/7,000 = 0.1735 = 17.35 cM
Correct D. ½×(617 + 641 + 2,297)/7,000 = ½×3,555/7,000 = 0.2539 = 25.39 cM
Incorrect E. ½×(2,274 + 2,297)/7,000 = ½×4,571/7,000 = 0.3265 = 32.65 cM
Incorrect F. ½×(564 + 607 + 617 + 2,274)/7,000 = ½×4,062/7,000 = 0.2901 = 29.01 cM
Incorrect MC

0133_da45

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + e e e e
7,405 
+ + e e + + e e
1,871 
+ + e e e e + +
1,762 
e e + + + + e e
1,809 
e e + + e e + +
1,796 
e e e e + + + +
7,357 
TOTAL22,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene E and its centromere.

A. ½×(1,796 + 7,405)/22,000 = ½×9,201/22,000 = 0.2091 = 20.91 cM
Incorrect B. ½×(1,762 + 1,796 + 1,809 + 7,357)/22,000 = ½×12,724/22,000 = 0.2892 = 28.92 cM
Incorrect C. ½×(1,762 + 1,796)/22,000 = ½×3,558/22,000 = 0.0809 = 8.09 cM
Incorrect D. ½×(1,762 + 1,809)/22,000 = ½×3,571/22,000 = 0.0812 = 8.12 cM
Incorrect E. ½×(1,762 + 1,796 + 1,809 + 1,871)/22,000 = ½×7,238/22,000 = 0.1645 = 16.45 cM
Correct F. ½×(7,357 + 7,405)/22,000 = ½×14,762/22,000 = 0.3355 = 33.55 cM
Incorrect MC

3751_253a

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + w w w w
3,559 
+ + w w + + w w
1,473 
+ + w w w w + +
1,430 
w w + + + + w w
1,448 
w w + + w w + +
1,525 
w w w w + + + +
3,565 
TOTAL13,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene W and its centromere.

A. ½×(1,525 + 3,565)/13,000 = ½×5,090/13,000 = 0.1958 = 19.58 cM
Incorrect B. ½×(1,430 + 1,448 + 1,473 + 3,559)/13,000 = ½×7,910/13,000 = 0.3042 = 30.42 cM
Incorrect C. ½×(1,448 + 1,525 + 3,559 + 3,565)/13,000 = ½×10,097/13,000 = 0.3883 = 38.83 cM
Incorrect D. ½×(1,448 + 1,473)/13,000 = ½×2,921/13,000 = 0.1123 = 11.23 cM
Incorrect E. ½×(1,430 + 1,448 + 1,473 + 1,525)/13,000 = ½×5,876/13,000 = 0.2260 = 22.60 cM
Correct F. ½×(3,559 + 3,565)/13,000 = ½×7,124/13,000 = 0.2740 = 27.40 cM
Incorrect MC

84cc_7fbb

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + e e e e
1,189 
+ + e e + + e e
430 
+ + e e e e + +
422 
e e + + + + e e
397 
e e + + e e + +
395 
e e e e + + + +
1,167 
TOTAL4,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene E and its centromere.

A. ½×(395 + 397 + 422 + 430)/4,000 = ½×1,644/4,000 = 0.2055 = 20.55 cM
Correct B. ½×(422 + 430)/4,000 = ½×852/4,000 = 0.1065 = 10.65 cM
Incorrect C. ½×(1,167 + 1,189)/4,000 = ½×2,356/4,000 = 0.2945 = 29.45 cM
Incorrect D. ½×(397 + 422)/4,000 = ½×819/4,000 = 0.1024 = 10.24 cM
Incorrect E. ½×(395 + 1,189)/4,000 = ½×1,584/4,000 = 0.1980 = 19.80 cM
Incorrect F. ½×(397 + 430)/4,000 = ½×827/4,000 = 0.1034 = 10.34 cM
Incorrect MC

1214_5c9b

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + t t t t
570 
+ + t t + + t t
108 
+ + t t t t + +
124 
t t + + + + t t
141 
t t + + t t + +
115 
t t t t + + + +
542 
TOTAL1,600
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene T and its centromere.

A. ½×(108 + 542 + 570)/1,600 = ½×1,220/1,600 = 0.3812 = 38.12 cM
Incorrect B. ½×(108 + 124 + 570)/1,600 = ½×802/1,600 = 0.2506 = 25.06 cM
Incorrect C. ½×(115 + 570)/1,600 = ½×685/1,600 = 0.2141 = 21.41 cM
Incorrect D. ½×(115 + 141 + 570)/1,600 = ½×826/1,600 = 0.2581 = 25.81 cM
Incorrect E. ½×(108 + 115 + 124 + 141)/1,600 = ½×488/1,600 = 0.1525 = 15.25 cM
Correct F. ½×(542 + 570)/1,600 = ½×1,112/1,600 = 0.3475 = 34.75 cM
Incorrect MC

0ff2_8a60

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + f f f f
1,055 
+ + f f + + f f
410 
+ + f f f f + +
433 
f f + + + + f f
429 
f f + + f f + +
419 
f f f f + + + +
1,054 
TOTAL3,800
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene F and its centromere.

A. ½×(410 + 419 + 1,054)/3,800 = ½×1,883/3,800 = 0.2478 = 24.78 cM
Incorrect B. ½×(410 + 419 + 429 + 433)/3,800 = ½×1,691/3,800 = 0.2225 = 22.25 cM
Correct C. ½×(429 + 433 + 1,054 + 1,055)/3,800 = ½×2,971/3,800 = 0.3909 = 39.09 cM
Incorrect D. ½×(1,054 + 1,055)/3,800 = ½×2,109/3,800 = 0.2775 = 27.75 cM
Incorrect E. ½×(419 + 429 + 1,054)/3,800 = ½×1,902/3,800 = 0.2503 = 25.03 cM
Incorrect F. ½×(429 + 1,055)/3,800 = ½×1,484/3,800 = 0.1953 = 19.53 cM
Incorrect MC

0297_6b38

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + d d d d
2,076 
+ + d d + + d d
244 
+ + d d d d + +
228 
d d + + + + d d
273 
d d + + d d + +
269 
d d d d + + + +
2,110 
TOTAL5,200
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene D and its centromere.

A. ½×(228 + 244 + 273 + 2,110)/5,200 = ½×2,855/5,200 = 0.2745 = 27.45 cM
Incorrect B. ½×(2,076 + 2,110)/5,200 = ½×4,186/5,200 = 0.4025 = 40.25 cM
Incorrect C. ½×(244 + 273 + 2,076 + 2,110)/5,200 = ½×4,703/5,200 = 0.4522 = 45.22 cM
Incorrect D. ½×(244 + 269)/5,200 = ½×513/5,200 = 0.0493 = 4.93 cM
Incorrect E. ½×(228 + 244 + 269 + 273)/5,200 = ½×1,014/5,200 = 0.0975 = 9.75 cM
Correct F. ½×(244 + 2,110)/5,200 = ½×2,354/5,200 = 0.2263 = 22.63 cM
Incorrect MC

a5da_8119

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + c c c c
1,720 
+ + c c + + c c
1,038 
+ + c c c c + +
1,023 
c c + + + + c c
1,032 
c c + + c c + +
987 
c c c c + + + +
1,700 
TOTAL7,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene C and its centromere.

A. ½×(1,023 + 1,032 + 1,700 + 1,720)/7,500 = ½×5,475/7,500 = 0.3650 = 36.50 cM
Incorrect B. ½×(1,023 + 1,038 + 1,700 + 1,720)/7,500 = ½×5,481/7,500 = 0.3654 = 36.54 cM
Incorrect C. ½×(987 + 1,032 + 1,700)/7,500 = ½×3,719/7,500 = 0.2479 = 24.79 cM
Incorrect D. ½×(987 + 1,023 + 1,032 + 1,038)/7,500 = ½×4,080/7,500 = 0.2720 = 27.20 cM
Correct E. ½×(1,700 + 1,720)/7,500 = ½×3,420/7,500 = 0.2280 = 22.80 cM
Incorrect F. ½×(987 + 1,720)/7,500 = ½×2,707/7,500 = 0.1805 = 18.05 cM
Incorrect MC

4416_a5e6

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + b b b b
1,845 
+ + b b + + b b
845 
+ + b b b b + +
871 
b b + + + + b b
845 
b b + + b b + +
806 
b b b b + + + +
1,788 
TOTAL7,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene B and its centromere.

A. ½×(1,788 + 1,845)/7,000 = ½×3,633/7,000 = 0.2595 = 25.95 cM
Incorrect B. ½×(806 + 845 + 1,788)/7,000 = ½×3,439/7,000 = 0.2456 = 24.56 cM
Incorrect C. ½×(845 + 871 + 1,788 + 1,845)/7,000 = ½×5,349/7,000 = 0.3821 = 38.21 cM
Incorrect D. ½×(845 + 845)/7,000 = ½×1,690/7,000 = 0.1207 = 12.07 cM
Incorrect E. ½×(806 + 845 + 845 + 871)/7,000 = ½×3,367/7,000 = 0.2405 = 24.05 cM
Correct F. ½×(845 + 1,845)/7,000 = ½×2,690/7,000 = 0.1921 = 19.21 cM
Incorrect MC

00d4_0755

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + w w w w
9,028 
+ + w w + + w w
1,778 
+ + w w w w + +
1,706 
w w + + + + w w
1,742 
w w + + w w + +
1,749 
w w w w + + + +
8,997 
TOTAL25,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene W and its centromere.

A. ½×(1,742 + 1,778)/25,000 = ½×3,520/25,000 = 0.0704 = 7.04 cM
Incorrect B. ½×(1,749 + 8,997)/25,000 = ½×10,746/25,000 = 0.2149 = 21.49 cM
Incorrect C. ½×(8,997 + 9,028)/25,000 = ½×18,025/25,000 = 0.3605 = 36.05 cM
Incorrect D. ½×(1,749 + 8,997 + 9,028)/25,000 = ½×19,774/25,000 = 0.3955 = 39.55 cM
Incorrect E. ½×(1,706 + 1,742 + 1,778 + 8,997)/25,000 = ½×14,223/25,000 = 0.2845 = 28.45 cM
Incorrect F. ½×(1,706 + 1,742 + 1,749 + 1,778)/25,000 = ½×6,975/25,000 = 0.1395 = 13.95 cM
Correct MC

e83b_bf85

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + w w w w
1,835 
+ + w w + + w w
966 
+ + w w w w + +
944 
w w + + + + w w
1,050 
w w + + w w + +
985 
w w w w + + + +
1,720 
TOTAL7,500
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene W and its centromere.

A. ½×(985 + 1,050)/7,500 = ½×2,035/7,500 = 0.1357 = 13.57 cM
Incorrect B. ½×(1,720 + 1,835)/7,500 = ½×3,555/7,500 = 0.2370 = 23.70 cM
Incorrect C. ½×(944 + 966 + 985 + 1,835)/7,500 = ½×4,730/7,500 = 0.3153 = 31.53 cM
Incorrect D. ½×(944 + 966 + 985 + 1,050)/7,500 = ½×3,945/7,500 = 0.2630 = 26.30 cM
Correct E. ½×(985 + 1,835)/7,500 = ½×2,820/7,500 = 0.1880 = 18.80 cM
Incorrect F. ½×(966 + 1,050 + 1,720 + 1,835)/7,500 = ½×5,571/7,500 = 0.3714 = 37.14 cM
Incorrect MC

afa1_cdf0

Ordered Tetrads in Neurospora crassa

Background Information

Neurospora crassa is an organism that has significantly contributed to the understanding of genetics. This fungus exhibits a distinctive genetic feature: the formation of ordered tetrads. These ordered tetrads result from the typical two rounds of meiotic divisions followed by a single round of mitotic division within an ascus, resulting in eight ascospores arranged in a predictable sequence. The position of each ascospore reflects the series of genetic events during cell division, providing a snapshot of the meiotic process.

The analysis of these ordered tetrads in Neurospora crassa allows for the classification of ascospores based on their allele arrangements into different segregation patterns.

The central principle in this analysis is the distinction between first-division and second-division segregation, which is based on the behavior of alleles in the presence or absence of crossover between a gene and its centromere. When alleles separate during the first meiotic division, it indicates first-division segregation. Conversely, if alleles separate during the second division, this suggests that a crossover event has occurred, leading to second-division segregation.

Counting the frequency of second-division segregation events within these ordered tetrads can provide an estimate of the genetic distance between a gene and its centromere. This frequency, reflective of the crossover events during meiosis, is used to calculate the recombination frequency. Such estimates are crucial for constructing genetic maps, which serve as a guide to the genetic landscape of Neurospora crassa, enhancing our understanding of genetic linkage and the location of genes relative to centromeres.

Experimental Data

In the table below, the six different patterns of ordered asci in Neurospora crassa are listed along with the counts found in an experiment.

OctadAsci
Count
+ + + + f f f f
677 
+ + f f + + f f
410 
+ + f f f f + +
389 
f f + + + + f f
411 
f f + + f f + +
413 
f f f f + + + +
700 
TOTAL3,000
Distance Formula
distance between a gene
and its centromere
=½ × (asci with second-division segregation patterns)
total number of asci
Question

Using the numbers of asci for each pattern shown in the table above, determine the genetic distance between gene F and its centromere.

A. ½×(389 + 677)/3,000 = ½×1,066/3,000 = 0.1777 = 17.77 cM
Incorrect B. ½×(677 + 700)/3,000 = ½×1,377/3,000 = 0.2295 = 22.95 cM
Incorrect C. ½×(410 + 411 + 700)/3,000 = ½×1,521/3,000 = 0.2535 = 25.35 cM
Incorrect D. ½×(411 + 700)/3,000 = ½×1,111/3,000 = 0.1852 = 18.52 cM
Incorrect E. ½×(410 + 413 + 700)/3,000 = ½×1,523/3,000 = 0.2538 = 25.38 cM
Incorrect F. ½×(389 + 410 + 411 + 413)/3,000 = ½×1,623/3,000 = 0.2705 = 27.05 cM
Correct