MA

6695_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. SdaI is derived from Streptomyces diastaticus Ng7-324, and MreI is derived from Micromonospora rhodorangea RFL1. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1SdaI 2 3 4MreI 5 6 7SdaI 8 9MreI 10SdaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SdaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

e9c4_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. VspI is derived from Vibrio species 343, and BclI is derived from Bacillus caldolyticus. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0VspI 1 2 3BclI 4VspI 5 6BclI 7 8 9 10VspI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with VspI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

f083_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. AscI is derived from Arthrobacter species, and MssI is derived from Methylobacterium species Dd 5-732. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1AscI 2 3MssI 4 5 6AscI 7MssI 8AscI 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AscI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

ea2e_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. BfaI is derived from Bacteroides fragilis, and MluI is derived from Micrococcus luteus. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0BfaI 1MluI 2 3 4 5BfaI 6 7 8MluI 9BfaI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BfaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

ce83_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. AseI is derived from Aquaspirillum serpens, and HpaI is derived from Haemophilus parainfluenzae. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0AseI 1 2HpaI 3 4AseI 5 6 7HpaI 8 9 10AseI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AseI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

5bbb_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. AanI is derived from Arthrobacter aurescens RFL2, and NsiI is derived from Neisseria sicca. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1AanI 2 3NsiI 4AanI 5 6 7NsiI 8 9AanI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AanI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

455c_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. PteI is derived from Paenibacillus terrae RFL2, and NsbI is derived from Neisseria subflava Va-1. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2PteI 3 4 5 6 7NsbI 8PteI 9NsbI 10PteI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PteI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

ea63_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. SgsI is derived from Streptomyces griseus RFL5, and PauI is derived from Paracoccus alcaliphilus ZVK3-3. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0SgsI 1 2PauI 3 4 5 6SgsI 7 8 9PauI 10SgsI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SgsI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

a247_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. BcuI is derived from Bacillus coagulans Vs 29-022, and PauI is derived from Paracoccus alcaliphilus ZVK3-3. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0BcuI 1PauI 2 3 4 5 6BcuI 7 8 9PauI 10BcuI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BcuI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

e6a0_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. MssI is derived from Methylobacterium species Dd 5-732, and FaeI is derived from Flavobacterium aquatile N3. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0MssI 1FaeI 2 3 4 5MssI 6 7FaeI 8MssI 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MssI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

c546_3635

Restriction enzymes are proteins that cut DNA at specific sequences. CfoI is derived from Clostridium formicoaceticum, and ScaI is derived from Streptomyces caespitosus. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3CfoI 4 5ScaI 6CfoI 7 8ScaI 9CfoI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with CfoI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Correct 5 kb Incorrect MA

c038_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. RsaI is derived from Rhodopseudomonas sphaeroides, and PmlI is derived from Pseudomonas maltophilia. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0RsaI 1 2PmlI 3 4RsaI 5 6 7PmlI 8 9RsaI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with RsaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

8eb0_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. NheI is derived from Neisseria mucosa, and VneI is derived from Vibrio nereis 18. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0NheI 1 2 3 4 5VneI 6 7NheI 8VneI 9 10NheI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NheI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

1a3c_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SspI is derived from Sphaerotilus species, and PaeI is derived from Pseudomonas aeruginosa. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1SspI 2 3PaeI 4SspI 5 6 7PaeI 8 9SspI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SspI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

3100_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. BfrI is derived from Bacteroides fragilis, and MseI is derived from Micrococcus species. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1BfrI 2 3MseI 4 5 6BfrI 7MseI 8 9 10BfrI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BfrI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

81e4_3635

Restriction enzymes are proteins that cut DNA at specific sequences. SrfI is derived from Streptomyces species Srf, and PaeI is derived from Pseudomonas aeruginosa. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0SrfI 1 2PaeI 3SrfI 4 5PaeI 6 7SrfI 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SrfI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

d81b_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. KroI is derived from Kocurea rosea 307, and MlsI is derived from Micrococcus luteus Ng 16-122. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3 4KroI 5MlsI 6KroI 7 8 9MlsI 10KroI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with KroI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

d04e_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. XmaI is derived from Xanthomonas malvacearum, and MspI is derived from Moraxella species ATCC 49670. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1XmaI 2 3 4MspI 5XmaI 6 7MspI 8 9 10XmaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with XmaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

df2f_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. MspI is derived from Moraxella species ATCC 49670, and BalI is derived from Brevibacterium albidum. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0MspI 1BalI 2MspI 3BalI 4 5 6MspI 7 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MspI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

a25d_3635

Restriction enzymes are proteins that cut DNA at specific sequences. NotI is derived from Nocardia otitidis-caviarum, and PscI is derived from Paracoccus species RFL1. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0NotI 1 2 3PscI 4NotI 5PscI 6 7NotI 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NotI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

38ee_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. BfaI is derived from Bacteroides fragilis, and MroI is derived from Micrococcus roseus. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0BfaI 1MroI 2 3BfaI 4 5MroI 6 7 8 9BfaI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BfaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

181c_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. NcoI is derived from Nocardia corallina, and HpaI is derived from Haemophilus parainfluenzae. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1NcoI 2 3 4HpaI 5 6 7NcoI 8 9HpaI 10NcoI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NcoI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

1cae_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. NarI is derived from Nocardia argentinensis, and SciI is derived from Streptoverticillium cinnamoneum. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1NarI 2 3 4SciI 5 6 7 8NarI 9SciI 10NarI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NarI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

ece5_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. PsiI is derived from Pseudomonas species SE-G49, and XbaI is derived from Xanthomonas badrii. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1PsiI 2 3XbaI 4 5PsiI 6 7XbaI 8 9 10PsiI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PsiI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

154a_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. MstI is derived from Microcoleus species, and ZrmI is derived from Zoogloea ramigera SCA. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3 4MstI 5 6ZrmI 7MstI 8ZrmI 9 10MstI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MstI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

c8b2_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. BalI is derived from Brevibacterium albidum, and ClaI is derived from Caryophanon latum L. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1BalI 2 3 4 5 6 7ClaI 8BalI 9ClaI 10BalI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BalI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

061a_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. CciI is derived from Curtobacterium citreus, and MvnI is derived from Methanococcus vannielii. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0CciI 1MvnI 2CciI 3 4 5 6MvnI 7CciI 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with CciI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

1d8c_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. MstI is derived from Microcoleus species, and NsiI is derived from Neisseria sicca. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0MstI 1 2 3NsiI 4MstI 5 6 7 8NsiI 9MstI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MstI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

88ee_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. PauI is derived from Paracoccus alcaliphilus ZVK3-3, and AseI is derived from Aquaspirillum serpens. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2PauI 3AseI 4PauI 5 6 7AseI 8 9PauI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PauI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

0b7d_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. SpeI is derived from Sphaerotilus natans, and PceI is derived from Planococcus citreus 55. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0SpeI 1 2 3 4 5PceI 6SpeI 7 8PceI 9 10SpeI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SpeI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

078a_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. TaiI is derived from Thermus aquaticus cs1-331, and BsnI is derived from Bacillus species SJN7. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1TaiI 2BsnI 3TaiI 4 5 6 7BsnI 8 9 10TaiI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with TaiI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

277f_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. BfrI is derived from Bacteroides fragilis, and DraI is derived from Deinococcus radiophilus. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0BfrI 1DraI 2BfrI 3 4 5 6DraI 7 8BfrI 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BfrI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

3dde_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. ApaI is derived from Acetobacter pasteurianus sub. pasteurianus, and NdeI is derived from Neisseria denitrificans. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1ApaI 2 3 4 5NdeI 6 7ApaI 8NdeI 9 10ApaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ApaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

2fe0_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. RigI is derived from Rhizobium yangligense, and SplI is derived from Spirulina platensis. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0RigI 1 2SplI 3 4RigI 5 6 7 8SplI 9 10RigI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with RigI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

e318_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. XbaI is derived from Xanthomonas badrii, and CfoI is derived from Clostridium formicoaceticum. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1XbaI 2CfoI 3 4XbaI 5 6CfoI 7 8 9 10XbaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with XbaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

f343_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. ScaI is derived from Streptomyces caespitosus, and RsaI is derived from Rhodopseudomonas sphaeroides. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3ScaI 4RsaI 5ScaI 6 7 8RsaI 9 10ScaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ScaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

ef0e_3635

Restriction enzymes are proteins that cut DNA at specific sequences. MspI is derived from Moraxella species ATCC 49670, and PceI is derived from Planococcus citreus 55. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1MspI 2 3PceI 4 5MspI 6 7PceI 8MspI 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MspI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Correct 5 kb Incorrect MA

48e4_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. PmeI is derived from Pseudomonas mendocina, and XbaI is derived from Xanthomonas badrii. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0PmeI 1 2 3XbaI 4 5 6 7PmeI 8 9XbaI 10PmeI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PmeI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

8bb2_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. KroI is derived from Kocurea rosea 307, and EgeI is derived from Enterobacter gergoviae NA. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0KroI 1 2EgeI 3 4 5KroI 6EgeI 7KroI 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with KroI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

ec05_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. MstI is derived from Microcoleus species, and SwaI is derived from Staphylococcus warneri. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0MstI 1SwaI 2 3 4 5 6 7MstI 8 9SwaI 10MstI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MstI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

ae7b_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. BfrI is derived from Bacteroides fragilis, and AscI is derived from Arthrobacter species. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2BfrI 3 4AscI 5 6 7 8BfrI 9AscI 10BfrI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BfrI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

8c59_3635

Restriction enzymes are proteins that cut DNA at specific sequences. MaeI is derived from Methanococcus aeolicus PL-15/H, and AfaI is derived from Acidiphilium facilis 28H. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1MaeI 2 3AfaI 4 5MaeI 6AfaI 7 8 9MaeI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MaeI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Incorrect MA

8095_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. AscI is derived from Arthrobacter species, and VneI is derived from Vibrio nereis 18. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0AscI 1 2VneI 3 4AscI 5 6 7VneI 8 9AscI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AscI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

47e7_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. PaeI is derived from Pseudomonas aeruginosa, and BcuI is derived from Bacillus coagulans Vs 29-022. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1PaeI 2 3BcuI 4 5 6 7PaeI 8BcuI 9PaeI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PaeI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

9fa1_3635

Restriction enzymes are proteins that cut DNA at specific sequences. MssI is derived from Methylobacterium species Dd 5-732, and CfoI is derived from Clostridium formicoaceticum. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1MssI 2CfoI 3 4 5MssI 6CfoI 7 8 9MssI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MssI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Incorrect MA

6e82_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SbfI is derived from Streptomyces species Bf-61, and NsbI is derived from Neisseria subflava Va-1. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1SbfI 2NsbI 3 4SbfI 5NsbI 6SbfI 7 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SbfI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

9ee5_3635

Restriction enzymes are proteins that cut DNA at specific sequences. NarI is derived from Nocardia argentinensis, and BsnI is derived from Bacillus species SJN7. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0NarI 1 2BsnI 3NarI 4 5BsnI 6 7NarI 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NarI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

f562_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SwaI is derived from Staphylococcus warneri, and NsiI is derived from Neisseria sicca. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0SwaI 1 2 3NsiI 4 5SwaI 6NsiI 7 8 9 10SwaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SwaI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

5747_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. BlnI is derived from Brevibacterium linens, and AluI is derived from Arthrobacter luteus. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3 4BlnI 5AluI 6BlnI 7AluI 8 9BlnI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BlnI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

278e_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. PabI is derived from Pyrococcus abyssi, and MalI is derived from Marinococus albus I. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1PabI 2 3 4MalI 5 6 7 8PabI 9MalI 10PabI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PabI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect