MC efc3_cff6
| 5′– | A | A | C | , | T | C | C | – | | 3′– | T | T | G | , | A | G | G | – | | | | – | C | A | C | , | C | G | T | , | C | A | C | , | T | A | T | , | C | A | C | , | T | T | A | , | G | C | C | , | T | A | T | – | | – | G | T | G | , | G | C | A | , | G | T | G | , | A | T | A | , | G | T | G | , | A | A | T | , | C | G | G | , | A | T | A | – | | | | – | T | G | A | , | G | T | C | –3′ | | – | A | C | T | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACTCC-3′ and 5′-GACTCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 5cc5_eb58
| 5′– | C | A | A | , | G | G | T | – | | 3′– | G | T | T | , | C | C | A | – | | | | – | G | T | C | , | C | G | A | , | T | C | C | , | A | G | C | , | T | G | G | , | A | T | C | , | A | A | C | , | C | G | T | – | | – | C | A | G | , | G | C | T | , | A | G | G | , | T | C | G | , | A | C | C | , | T | A | G | , | T | T | G | , | G | C | A | – | | | | – | G | G | A | , | G | A | T | –3′ | | – | C | C | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAAGGT-3′ and 5′-ATCTCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC f61a_9683
| 5′– | T | A | A | , | G | A | C | – | | 3′– | A | T | T | , | C | T | G | – | | | | – | T | T | T | , | G | C | A | , | G | G | C | , | T | C | C | , | C | T | G | , | A | T | C | , | A | T | A | , | C | G | G | – | | – | A | A | A | , | C | G | T | , | C | C | G | , | A | G | G | , | G | A | C | , | T | A | G | , | T | A | T | , | G | C | C | – | | | | – | G | A | A | , | G | A | T | –3′ | | – | C | T | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAAGAC-3′ and 5′-ATCTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7384_691c
| 5′– | A | G | C | , | T | A | C | – | | 3′– | T | C | G | , | A | T | G | – | | | | – | T | C | T | , | A | A | G | , | T | A | C | , | T | A | T | , | C | A | G | , | G | A | T | , | T | G | A | , | T | G | C | – | | – | A | G | A | , | T | T | C | , | A | T | G | , | A | T | A | , | G | T | C | , | C | T | A | , | A | C | T | , | A | C | G | – | | | | – | G | T | C | , | C | C | A | –3′ | | – | C | A | G | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGCTAC-3′ and 5′-TGGGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 4872_afcd
| 5′– | C | C | G | , | A | G | T | – | | 3′– | G | G | C | , | T | C | A | – | | | | – | A | A | C | , | A | G | T | , | C | T | A | , | C | C | A | , | A | C | G | , | G | T | G | , | T | C | A | , | A | T | G | – | | – | T | T | G | , | T | C | A | , | G | A | T | , | G | G | T | , | T | G | C | , | C | A | C | , | A | G | T | , | T | A | C | – | | | | – | G | T | T | , | G | C | A | –3′ | | – | C | A | A | , | C | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCGAGT-3′ and 5′-TGCAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 69ab_8530
| 5′– | C | G | T | , | G | A | A | – | | 3′– | G | C | A | , | C | T | T | – | | | | – | T | A | G | , | C | T | G | , | T | T | C | , | T | C | G | , | A | C | G | , | C | T | T | , | C | T | C | , | A | C | G | – | | – | A | T | C | , | G | A | C | , | A | A | G | , | A | G | C | , | T | G | C | , | G | A | A | , | G | A | G | , | T | G | C | – | | | | – | C | A | G | , | G | T | T | –3′ | | – | G | T | C | , | C | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTGAA-3′ and 5′-AACCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 3580_c1b0
| 5′– | A | G | T | , | A | A | C | – | | 3′– | T | C | A | , | T | T | G | – | | | | – | T | C | G | , | G | A | C | , | A | C | A | , | A | A | C | , | A | T | C | , | A | C | G | , | A | T | G | , | T | C | C | – | | – | A | G | C | , | C | T | G | , | T | G | T | , | T | T | G | , | T | A | G | , | T | G | C | , | T | A | C | , | A | G | G | – | | | | – | T | C | A | , | T | A | G | –3′ | | – | A | G | T | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTAAC-3′ and 5′-CTATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC a163_33dd
| 5′– | C | A | A | , | G | C | T | – | | 3′– | G | T | T | , | C | G | A | – | | | | – | G | C | A | , | T | A | C | , | G | T | G | , | A | G | T | , | A | T | G | , | C | T | G | , | T | G | A | , | G | C | A | – | | – | C | G | T | , | A | T | G | , | C | A | C | , | T | C | A | , | T | A | C | , | G | A | C | , | A | C | T | , | C | G | T | – | | | | – | G | G | A | , | T | C | A | –3′ | | – | C | C | T | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAAGCT-3′ and 5′-TGATCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 08f7_a474
| 5′– | G | T | A | , | A | C | T | – | | 3′– | C | A | T | , | T | G | A | – | | | | – | T | C | G | , | A | T | A | , | C | C | T | , | A | C | C | , | G | T | C | , | T | A | A | , | C | T | G | , | A | C | C | – | | – | A | G | C | , | T | A | T | , | G | G | A | , | T | G | G | , | C | A | G | , | A | T | T | , | G | A | C | , | T | G | G | – | | | | – | C | T | G | , | G | T | A | –3′ | | – | G | A | C | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTAACT-3′ and 5′-TACCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC d1bc_e592
| 5′– | C | A | T | , | C | A | A | – | | 3′– | G | T | A | , | G | T | T | – | | | | – | G | T | G | , | C | T | A | , | C | G | T | , | T | C | C | , | C | T | G | , | T | A | A | , | G | T | A | , | C | A | T | – | | – | C | A | C | , | G | A | T | , | G | C | A | , | A | G | G | , | G | A | C | , | A | T | T | , | C | A | T | , | G | T | A | – | | | | – | A | A | T | , | C | G | G | –3′ | | – | T | T | A | , | G | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATCAA-3′ and 5′-CCGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC d7cc_6b01
| 5′– | A | C | G | , | T | C | G | – | | 3′– | T | G | C | , | A | G | C | – | | | | – | G | T | C | , | T | G | A | , | T | C | C | , | C | A | A | , | A | G | G | , | T | G | T | , | T | G | C | , | A | C | T | – | | – | C | A | G | , | A | C | T | , | A | G | G | , | G | T | T | , | T | C | C | , | A | C | A | , | A | C | G | , | T | G | A | – | | | | – | T | T | A | , | G | C | C | –3′ | | – | A | A | T | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACGTCG-3′ and 5′-GGCTAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 9f7d_6f7b
| 5′– | C | T | G | , | C | G | A | – | | 3′– | G | A | C | , | G | C | T | – | | | | – | G | A | G | , | C | C | T | , | C | C | C | , | A | G | T | , | G | C | A | , | G | T | C | , | T | C | G | , | A | C | A | – | | – | C | T | C | , | G | G | A | , | G | G | G | , | T | C | A | , | C | G | T | , | C | A | G | , | A | G | C | , | T | G | T | – | | | | – | A | T | T | , | C | T | G | –3′ | | – | T | A | A | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGCGA-3′ and 5′-CAGAAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0c24_262d
| 5′– | G | C | T | , | G | T | A | – | | 3′– | C | G | A | , | C | A | T | – | | | | – | C | A | G | , | G | G | T | , | G | T | C | , | T | T | C | , | C | A | T | , | G | G | A | , | A | G | A | , | C | T | A | – | | – | G | T | C | , | C | C | A | , | C | A | G | , | A | A | G | , | G | T | A | , | C | C | T | , | T | C | T | , | G | A | T | – | | | | – | G | T | A | , | C | C | T | –3′ | | – | C | A | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTGTA-3′ and 5′-AGGTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC cec1_68e3
| 5′– | T | G | C | , | C | A | G | – | | 3′– | A | C | G | , | G | T | C | – | | | | – | G | T | T | , | G | C | A | , | G | A | T | , | T | C | T | , | T | C | C | , | G | A | C | , | T | C | G | , | C | A | T | – | | – | C | A | A | , | C | G | T | , | C | T | A | , | A | G | A | , | A | G | G | , | C | T | G | , | A | G | C | , | G | T | A | – | | | | – | T | G | G | , | A | C | G | –3′ | | – | A | C | C | , | T | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCCAG-3′ and 5′-CGTCCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a4b2_1582
| 5′– | T | G | A | , | G | C | C | – | | 3′– | A | C | T | , | C | G | G | – | | | | – | G | C | C | , | A | T | A | , | G | A | A | , | T | G | T | , | T | T | C | , | A | C | T | , | C | A | G | , | T | C | T | – | | – | C | G | G | , | T | A | T | , | C | T | T | , | A | C | A | , | A | A | G | , | T | G | A | , | G | T | C | , | A | G | A | – | | | | – | G | A | T | , | C | A | T | –3′ | | – | C | T | A | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAGCC-3′ and 5′-ATGATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 900b_456d
| 5′– | A | T | C | , | A | T | G | – | | 3′– | T | A | G | , | T | A | C | – | | | | – | T | T | A | , | C | T | G | , | A | A | C | , | G | G | A | , | C | G | T | , | A | C | G | , | A | T | G | , | C | T | C | – | | – | A | A | T | , | G | A | C | , | T | T | G | , | C | C | T | , | G | C | A | , | T | G | C | , | T | A | C | , | G | A | G | – | | | | – | T | T | G | , | A | T | C | –3′ | | – | A | A | C | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCATG-3′ and 5′-GATCAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f5be_cc00
| 5′– | A | C | C | , | A | T | C | – | | 3′– | T | G | G | , | T | A | G | – | | | | – | G | A | C | , | C | G | T | , | C | A | G | , | A | A | C | , | A | A | A | , | C | G | C | , | A | G | G | , | A | C | T | – | | – | C | T | G | , | G | C | A | , | G | T | C | , | T | T | G | , | T | T | T | , | G | C | G | , | T | C | C | , | T | G | A | – | | | | – | A | C | T | , | A | T | G | –3′ | | – | T | G | A | , | T | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACCATC-3′ and 5′-CATAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC c4bb_d3bd
| 5′– | C | C | G | , | A | G | T | – | | 3′– | G | G | C | , | T | C | A | – | | | | – | T | A | G | , | C | G | T | , | A | C | C | , | C | T | G | , | G | C | T | , | T | C | A | , | C | G | T | , | T | A | G | – | | – | A | T | C | , | G | C | A | , | T | G | G | , | G | A | C | , | C | G | A | , | A | G | T | , | G | C | A | , | A | T | C | – | | | | – | G | A | C | , | G | G | T | –3′ | | – | C | T | G | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCGAGT-3′ and 5′-ACCGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 4832_22b7
| 5′– | C | C | T | , | G | A | T | – | | 3′– | G | G | A | , | C | T | A | – | | | | – | G | A | T | , | C | T | G | , | T | T | C | , | C | A | G | , | G | A | G | , | A | C | C | , | T | C | A | , | G | A | T | – | | – | C | T | A | , | G | A | C | , | A | A | G | , | G | T | C | , | C | T | C | , | T | G | G | , | A | G | T | , | C | T | A | – | | | | – | T | C | A | , | A | C | C | –3′ | | – | A | G | T | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTGAT-3′ and 5′-GGTTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 025c_e6bc
| 5′– | T | A | G | , | A | C | C | – | | 3′– | A | T | C | , | T | G | G | – | | | | – | C | T | T | , | T | G | A | , | A | G | C | , | G | T | C | , | G | C | A | , | G | A | T | , | A | C | G | , | A | T | T | – | | – | G | A | A | , | A | C | T | , | T | C | G | , | C | A | G | , | C | G | T | , | C | T | A | , | T | G | C | , | T | A | A | – | | | | – | T | A | C | , | A | T | C | –3′ | | – | A | T | G | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGACC-3′ and 5′-GATGTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC f2ea_fdd0
| 5′– | A | G | T | , | G | A | C | – | | 3′– | T | C | A | , | C | T | G | – | | | | – | C | G | A | , | G | C | T | , | C | C | C | , | T | T | C | , | T | T | G | , | T | A | C | , | C | C | T | , | A | G | T | – | | – | G | C | T | , | C | G | A | , | G | G | G | , | A | A | G | , | A | A | C | , | A | T | G | , | G | G | A | , | T | C | A | – | | | | – | A | T | G | , | C | A | G | –3′ | | – | T | A | C | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTGAC-3′ and 5′-CTGCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 7c2b_9384
| 5′– | A | G | G | , | T | G | G | – | | 3′– | T | C | C | , | A | C | C | – | | | | – | T | C | A | , | T | A | G | , | G | A | G | , | A | G | G | , | A | A | C | , | A | T | C | , | G | T | T | , | A | C | C | – | | – | A | G | T | , | A | T | C | , | C | T | C | , | T | C | C | , | T | T | G | , | T | A | G | , | C | A | A | , | T | G | G | – | | | | – | G | C | C | , | T | A | A | –3′ | | – | C | G | G | , | A | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTGG-3′ and 5′-TTAGGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC cc56_0121
| 5′– | C | G | T | , | T | G | A | – | | 3′– | G | C | A | , | A | C | T | – | | | | – | A | G | C | , | A | T | A | , | G | C | T | , | C | A | A | , | G | A | G | , | G | C | A | , | T | C | G | , | G | A | C | – | | – | T | C | G | , | T | A | T | , | C | G | A | , | G | T | T | , | C | T | C | , | C | G | T | , | A | G | C | , | C | T | G | – | | | | – | C | C | G | , | T | T | A | –3′ | | – | G | G | C | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTTGA-3′ and 5′-TAACGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 9351_caec
| 5′– | G | C | C | , | T | C | A | – | | 3′– | C | G | G | , | A | G | T | – | | | | – | C | A | T | , | C | G | T | , | C | T | C | , | T | T | C | , | C | T | A | , | C | A | C | , | C | G | A | , | C | T | T | – | | – | G | T | A | , | G | C | A | , | G | A | G | , | A | A | G | , | G | A | T | , | G | T | G | , | G | C | T | , | G | A | A | – | | | | – | T | G | G | , | A | T | G | –3′ | | – | A | C | C | , | T | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCCTCA-3′ and 5′-CATCCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC d3a3_06d3
| 5′– | C | T | A | , | C | T | T | – | | 3′– | G | A | T | , | G | A | A | – | | | | – | T | C | A | , | G | T | G | , | T | G | C | , | A | A | T | , | C | T | G | , | A | C | T | , | C | G | A | , | C | T | C | – | | – | A | G | T | , | C | A | C | , | A | C | G | , | T | T | A | , | G | A | C | , | T | G | A | , | G | C | T | , | G | A | G | – | | | | – | T | A | G | , | A | C | C | –3′ | | – | A | T | C | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTACTT-3′ and 5′-GGTCTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 74c5_27dd
| 5′– | T | T | C | , | G | A | C | – | | 3′– | A | A | G | , | C | T | G | – | | | | – | A | T | G | , | C | T | C | , | G | G | T | , | A | A | G | , | G | G | A | , | T | G | G | , | T | C | A | , | C | A | G | – | | – | T | A | C | , | G | A | G | , | C | C | A | , | T | T | C | , | C | C | T | , | A | C | C | , | A | G | T | , | G | T | C | – | | | | – | C | C | T | , | G | A | A | –3′ | | – | G | G | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCGAC-3′ and 5′-TTCAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b1bd_4256
| 5′– | A | G | T | , | A | A | C | – | | 3′– | T | C | A | , | T | T | G | – | | | | – | T | C | A | , | G | C | C | , | A | A | G | , | C | C | A | , | T | C | A | , | C | T | A | , | C | A | T | , | T | G | G | – | | – | A | G | T | , | C | G | G | , | T | T | C | , | G | G | T | , | A | G | T | , | G | A | T | , | G | T | A | , | A | C | C | – | | | | – | A | C | T | , | C | A | G | –3′ | | – | T | G | A | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTAAC-3′ and 5′-CTGAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 236f_c8f6
| 5′– | G | C | C | , | T | T | A | – | | 3′– | C | G | G | , | A | A | T | – | | | | – | A | C | T | , | G | A | G | , | C | G | A | , | A | T | C | , | G | C | A | , | T | T | G | , | C | G | A | , | A | T | G | – | | – | T | G | A | , | C | T | C | , | G | C | T | , | T | A | G | , | C | G | T | , | A | A | C | , | G | C | T | , | T | A | C | – | | | | – | T | G | A | , | C | A | G | –3′ | | – | A | C | T | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCCTTA-3′ and 5′-CTGTCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC e40f_4bc3
| 5′– | T | C | A | , | G | C | C | – | | 3′– | A | G | T | , | C | G | G | – | | | | – | G | A | C | , | A | T | C | , | T | A | G | , | T | G | A | , | C | C | A | , | C | C | G | , | T | A | C | , | G | C | T | – | | – | C | T | G | , | T | A | G | , | A | T | C | , | A | C | T | , | G | G | T | , | G | G | C | , | A | T | G | , | C | G | A | – | | | | – | A | T | G | , | A | C | G | –3′ | | – | T | A | C | , | T | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAGCC-3′ and 5′-CGTCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC e9a4_1a9c
| 5′– | C | T | T | , | A | G | T | – | | 3′– | G | A | A | , | T | C | A | – | | | | – | A | G | T | , | C | G | T | , | T | C | A | , | C | T | A | , | A | C | A | , | T | C | G | , | A | A | T | , | C | C | G | – | | – | T | C | A | , | G | C | A | , | A | G | T | , | G | A | T | , | T | G | T | , | A | G | C | , | T | T | A | , | G | G | C | – | | | | – | A | C | G | , | C | T | G | –3′ | | – | T | G | C | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTTAGT-3′ and 5′-CAGCGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 5149_7bcb
| 5′– | T | C | A | , | A | T | C | – | | 3′– | A | G | T | , | T | A | G | – | | | | – | T | C | G | , | A | C | A | , | A | G | A | , | G | T | A | , | G | G | A | , | T | G | T | , | A | C | T | , | T | A | G | – | | – | A | G | C | , | T | G | T | , | T | C | T | , | C | A | T | , | C | C | T | , | A | C | A | , | T | G | A | , | A | T | C | – | | | | – | C | T | G | , | C | A | T | –3′ | | – | G | A | C | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAATC-3′ and 5′-ATGCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7977_7dc4
| 5′– | T | G | A | , | T | A | G | – | | 3′– | A | C | T | , | A | T | C | – | | | | – | T | G | G | , | C | A | T | , | C | A | A | , | A | C | A | , | C | G | C | , | A | G | A | , | C | T | A | , | A | C | G | – | | – | A | C | C | , | G | T | A | , | G | T | T | , | T | G | T | , | G | C | G | , | T | C | T | , | G | A | T | , | T | G | C | – | | | | – | A | T | C | , | A | A | C | –3′ | | – | T | A | G | , | T | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGATAG-3′ and 5′-GTTGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ee75_f7c5
| 5′– | C | T | T | , | A | C | T | – | | 3′– | G | A | A | , | T | G | A | – | | | | – | G | T | G | , | A | C | C | , | A | C | C | , | A | T | C | , | A | A | C | , | A | G | C | , | T | C | A | , | G | A | T | – | | – | C | A | C | , | T | G | G | , | T | G | G | , | T | A | G | , | T | T | G | , | T | C | G | , | A | G | T | , | C | T | A | – | | | | – | T | T | A | , | G | T | C | –3′ | | – | A | A | T | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTTACT-3′ and 5′-GACTAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC bc8f_98b6
| 5′– | G | C | T | , | A | C | T | – | | 3′– | C | G | A | , | T | G | A | – | | | | – | G | G | A | , | C | T | C | , | A | G | T | , | C | G | A | , | T | C | G | , | T | A | G | , | C | A | A | , | C | G | T | – | | – | C | C | T | , | G | A | G | , | T | C | A | , | G | C | T | , | A | G | C | , | A | T | C | , | G | T | T | , | G | C | A | – | | | | – | A | C | T | , | T | T | G | –3′ | | – | T | G | A | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTACT-3′ and 5′-CAAAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 29ba_beca
| 5′– | C | G | T | , | C | G | A | – | | 3′– | G | C | A | , | G | C | T | – | | | | – | A | T | G | , | G | A | C | , | T | C | G | , | C | A | T | , | A | G | A | , | T | G | A | , | G | C | A | , | G | T | G | – | | – | T | A | C | , | C | T | G | , | A | G | C | , | G | T | A | , | T | C | T | , | A | C | T | , | C | G | T | , | C | A | C | – | | | | – | C | C | A | , | C | T | A | –3′ | | – | G | G | T | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTCGA-3′ and 5′-TAGTGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC e0f8_5ea7
| 5′– | T | C | C | , | C | A | G | – | | 3′– | A | G | G | , | G | T | C | – | | | | – | G | C | A | , | C | T | A | , | G | G | T | , | T | A | C | , | A | A | G | , | A | C | G | , | A | A | T | , | G | C | A | – | | – | C | G | T | , | G | A | T | , | C | C | A | , | A | T | G | , | T | T | C | , | T | G | C | , | T | T | A | , | C | G | T | – | | | | – | A | G | T | , | A | C | C | –3′ | | – | T | C | A | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCCCAG-3′ and 5′-GGTACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC bca9_d07e
| 5′– | T | T | G | , | A | T | C | – | | 3′– | A | A | C | , | T | A | G | – | | | | – | G | G | C | , | A | C | T | , | G | C | A | , | C | T | T | , | C | A | A | , | C | C | A | , | A | C | G | , | T | T | T | – | | – | C | C | G | , | T | G | A | , | C | G | T | , | G | A | A | , | G | T | T | , | G | G | T | , | T | G | C | , | A | A | A | – | | | | – | A | G | T | , | A | G | G | –3′ | | – | T | C | A | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTGATC-3′ and 5′-CCTACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0d8f_51f8
| 5′– | A | G | T | , | C | A | G | – | | 3′– | T | C | A | , | G | T | C | – | | | | – | T | C | C | , | G | A | T | , | T | C | G | , | G | T | G | , | A | C | A | , | G | T | A | , | C | C | A | , | C | T | G | – | | – | A | G | G | , | C | T | A | , | A | G | C | , | C | A | C | , | T | G | T | , | C | A | T | , | G | G | T | , | G | A | C | – | | | | – | G | A | A | , | G | C | T | –3′ | | – | C | T | T | , | C | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTCAG-3′ and 5′-AGCTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC a56e_b4b2
| 5′– | T | G | C | , | A | A | G | – | | 3′– | A | C | G | , | T | T | C | – | | | | – | G | C | G | , | A | A | T | , | G | T | C | , | G | A | A | , | G | C | T | , | A | C | G | , | T | C | T | , | G | G | A | – | | – | C | G | C | , | T | T | A | , | C | A | G | , | C | T | T | , | C | G | A | , | T | G | C | , | A | G | A | , | C | C | T | – | | | | – | T | T | A | , | C | C | G | –3′ | | – | A | A | T | , | G | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCAAG-3′ and 5′-CGGTAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 05dc_98c6
| 5′– | A | T | C | , | A | A | C | – | | 3′– | T | A | G | , | T | T | G | – | | | | – | C | T | A | , | T | G | G | , | G | A | T | , | G | G | T | , | A | C | G | , | T | A | T | , | C | A | A | , | A | G | T | – | | – | G | A | T | , | A | C | C | , | C | T | A | , | C | C | A | , | T | G | C | , | A | T | A | , | G | T | T | , | T | C | A | – | | | | – | G | G | C | , | T | C | A | –3′ | | – | C | C | G | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCAAC-3′ and 5′-TGAGCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5cfd_c769
| 5′– | T | G | A | , | C | A | G | – | | 3′– | A | C | T | , | G | T | C | – | | | | – | T | G | G | , | T | C | A | , | T | C | G | , | A | C | G | , | T | A | G | , | A | C | T | , | G | A | T | , | G | C | C | – | | – | A | C | C | , | A | G | T | , | A | G | C | , | T | G | C | , | A | T | C | , | T | G | A | , | C | T | A | , | C | G | G | – | | | | – | T | G | C | , | T | A | C | –3′ | | – | A | C | G | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGACAG-3′ and 5′-GTAGCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f555_f63c
| 5′– | T | C | A | , | G | T | C | – | | 3′– | A | G | T | , | C | A | G | – | | | | – | A | C | C | , | G | T | A | , | A | G | T | , | G | G | A | , | A | G | A | , | G | G | C | , | A | C | T | , | A | A | G | – | | – | T | G | G | , | C | A | T | , | T | C | A | , | C | C | T | , | T | C | T | , | C | C | G | , | T | G | A | , | T | T | C | – | | | | – | C | C | T | , | G | A | A | –3′ | | – | G | G | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAGTC-3′ and 5′-TTCAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4ac4_062a
| 5′– | G | A | T | , | C | A | A | – | | 3′– | C | T | A | , | G | T | T | – | | | | – | A | A | C | , | C | G | T | , | G | T | G | , | T | C | T | , | C | A | A | , | A | C | T | , | A | G | C | , | T | C | G | – | | – | T | T | G | , | G | C | A | , | C | A | C | , | A | G | A | , | G | T | T | , | T | G | A | , | T | C | G | , | A | G | C | – | | | | – | G | A | C | , | G | G | T | –3′ | | – | C | T | G | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATCAA-3′ and 5′-ACCGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 8133_a5da
| 5′– | A | G | T | , | A | T | C | – | | 3′– | T | C | A | , | T | A | G | – | | | | – | A | A | G | , | C | T | C | , | A | A | G | , | G | A | T | , | G | C | A | , | C | C | A | , | C | A | T | , | T | G | C | – | | – | T | T | C | , | G | A | G | , | T | T | C | , | C | T | A | , | C | G | T | , | G | G | T | , | G | T | A | , | A | C | G | – | | | | – | C | A | A | , | G | G | T | –3′ | | – | G | T | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTATC-3′ and 5′-ACCTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 9e90_31b3
| 5′– | A | T | G | , | T | A | G | – | | 3′– | T | A | C | , | A | T | C | – | | | | – | G | T | A | , | C | C | A | , | C | T | T | , | G | C | G | , | T | C | A | , | T | C | A | , | A | G | C | , | C | A | T | – | | – | C | A | T | , | G | G | T | , | G | A | A | , | C | G | C | , | A | G | T | , | A | G | T | , | T | C | G | , | G | T | A | – | | | | – | C | G | A | , | A | C | T | –3′ | | – | G | C | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGTAG-3′ and 5′-AGTTCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 92a7_ef8c
| 5′– | C | T | G | , | C | A | A | – | | 3′– | G | A | C | , | G | T | T | – | | | | – | G | T | A | , | A | C | T | , | C | A | G | , | C | T | T | , | T | C | A | , | A | G | A | , | A | A | C | , | A | G | T | – | | – | C | A | T | , | T | G | A | , | G | T | C | , | G | A | A | , | A | G | T | , | T | C | T | , | T | T | G | , | T | C | A | – | | | | – | T | T | C | , | A | G | C | –3′ | | – | A | A | G | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGCAA-3′ and 5′-GCTGAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC e512_1b1a
| 5′– | G | T | C | , | G | C | A | – | | 3′– | C | A | G | , | C | G | T | – | | | | – | C | A | C | , | T | G | A | , | T | G | G | , | T | C | G | , | A | G | T | , | G | G | C | , | A | G | T | , | G | C | A | – | | – | G | T | G | , | A | C | T | , | A | C | C | , | A | G | C | , | T | C | A | , | C | C | G | , | T | C | A | , | C | G | T | – | | | | – | C | G | T | , | G | A | A | –3′ | | – | G | C | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCGCA-3′ and 5′-TTCACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC d670_1577
| 5′– | G | A | T | , | C | C | A | – | | 3′– | C | T | A | , | G | G | T | – | | | | – | C | T | C | , | G | C | A | , | T | C | C | , | A | G | A | , | G | A | G | , | G | G | T | , | C | G | T | , | A | G | T | – | | – | G | A | G | , | C | G | T | , | A | G | G | , | T | C | T | , | C | T | C | , | C | C | A | , | G | C | A | , | T | C | A | – | | | | – | G | A | C | , | T | C | A | –3′ | | – | C | T | G | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATCCA-3′ and 5′-TGAGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC a6d8_f0c2
| 5′– | C | G | G | , | A | C | T | – | | 3′– | G | C | C | , | T | G | A | – | | | | – | A | C | A | , | G | T | C | , | A | G | A | , | A | G | A | , | G | A | A | , | T | C | A | , | C | A | T | , | G | G | G | – | | – | T | G | T | , | C | A | G | , | T | C | T | , | T | C | T | , | C | T | T | , | A | G | T | , | G | T | A | , | C | C | C | – | | | | – | C | T | G | , | C | A | A | –3′ | | – | G | A | C | , | G | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGGACT-3′ and 5′-TTGCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC b7fd_cc57
| 5′– | G | A | A | , | A | G | T | – | | 3′– | C | T | T | , | T | C | A | – | | | | – | C | C | G | , | T | A | G | , | C | T | G | , | A | C | G | , | T | G | A | , | T | T | C | , | A | C | A | , | G | A | T | – | | – | G | G | C | , | A | T | C | , | G | A | C | , | T | G | C | , | A | C | T | , | A | A | G | , | T | G | T | , | C | T | A | – | | | | – | A | A | T | , | C | T | G | –3′ | | – | T | T | A | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GAAAGT-3′ and 5′-CAGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC c828_9137
| 5′– | A | A | T | , | G | G | C | – | | 3′– | T | T | A | , | C | C | G | – | | | | – | C | G | T | , | G | A | T | , | G | A | G | , | G | T | C | , | T | C | T | , | G | T | T | , | T | C | G | , | T | T | A | – | | – | G | C | A | , | C | T | A | , | C | T | C | , | C | A | G | , | A | G | A | , | C | A | A | , | A | G | C | , | A | A | T | – | | | | – | C | A | A | , | C | C | T | –3′ | | – | G | T | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AATGGC-3′ and 5′-AGGTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5572_ec9b
| 5′– | A | A | C | , | T | T | C | – | | 3′– | T | T | G | , | A | A | G | – | | | | – | T | G | A | , | C | C | T | , | G | T | A | , | G | T | A | , | C | A | G | , | G | C | A | , | T | C | C | , | A | G | G | – | | – | A | C | T | , | G | G | A | , | C | A | T | , | C | A | T | , | G | T | C | , | C | G | T | , | A | G | G | , | T | C | C | – | | | | – | G | T | T | , | G | T | A | –3′ | | – | C | A | A | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACTTC-3′ and 5′-TACAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 2622_cb42
| 5′– | T | G | G | , | A | A | C | – | | 3′– | A | C | C | , | T | T | G | – | | | | – | G | T | T | , | C | T | A | , | G | C | T | , | G | T | A | , | C | A | T | , | G | T | C | , | C | T | G | , | G | A | T | – | | – | C | A | A | , | G | A | T | , | C | G | A | , | C | A | T | , | G | T | A | , | C | A | G | , | G | A | C | , | C | T | A | – | | | | – | C | C | T | , | A | C | T | –3′ | | – | G | G | A | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGAAC-3′ and 5′-AGTAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 43bf_b081
| 5′– | G | T | A | , | C | C | T | – | | 3′– | C | A | T | , | G | G | A | – | | | | – | G | T | A | , | A | C | G | , | G | T | T | , | C | T | G | , | T | A | G | , | T | G | C | , | C | T | G | , | A | A | A | – | | – | C | A | T | , | T | G | C | , | C | A | A | , | G | A | C | , | A | T | C | , | A | C | G | , | G | A | C | , | T | T | T | – | | | | – | C | A | T | , | C | G | T | –3′ | | – | G | T | A | , | G | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTACCT-3′ and 5′-ACGATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a8b5_796d
| 5′– | T | T | A | , | G | G | C | – | | 3′– | A | A | T | , | C | C | G | – | | | | – | G | A | T | , | G | T | C | , | T | G | A | , | T | G | A | , | A | A | G | , | G | T | T | , | G | G | A | , | C | A | T | – | | – | C | T | A | , | C | A | G | , | A | C | T | , | A | C | T | , | T | T | C | , | C | A | A | , | C | C | T | , | G | T | A | – | | | | – | G | T | C | , | T | A | A | –3′ | | – | C | A | G | , | A | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTAGGC-3′ and 5′-TTAGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 18fd_dbaa
| 5′– | G | C | C | , | T | G | A | – | | 3′– | C | G | G | , | A | C | T | – | | | | – | C | G | T | , | C | A | A | , | G | A | G | , | T | G | T | , | C | T | T | , | T | G | C | , | C | A | G | , | A | G | T | – | | – | G | C | A | , | G | T | T | , | C | T | C | , | A | C | A | , | G | A | A | , | A | C | G | , | G | T | C | , | T | C | A | – | | | | – | G | A | C | , | A | G | T | –3′ | | – | C | T | G | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCCTGA-3′ and 5′-ACTGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC d1e2_8bd5
| 5′– | G | A | C | , | C | T | T | – | | 3′– | C | T | G | , | G | A | A | – | | | | – | T | G | G | , | G | A | C | , | T | T | C | , | T | G | C | , | C | A | G | , | T | A | A | , | G | T | C | , | A | C | C | – | | – | A | C | C | , | C | T | G | , | A | A | G | , | A | C | G | , | G | T | C | , | A | T | T | , | C | A | G | , | T | G | G | – | | | | – | A | G | G | , | T | A | C | –3′ | | – | T | C | C | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACCTT-3′ and 5′-GTACCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC e9fd_de6c
| 5′– | T | C | A | , | T | A | C | – | | 3′– | A | G | T | , | A | T | G | – | | | | – | C | G | A | , | G | A | T | , | A | C | G | , | A | G | A | , | C | A | T | , | C | C | G | , | T | G | C | , | G | A | A | – | | – | G | C | T | , | C | T | A | , | T | G | C | , | T | C | T | , | G | T | A | , | G | G | C | , | A | C | G | , | C | T | T | – | | | | – | C | T | G | , | G | C | A | –3′ | | – | G | A | C | , | C | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCATAC-3′ and 5′-TGCCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 9bcc_0b94
| 5′– | T | G | C | , | T | A | G | – | | 3′– | A | C | G | , | A | T | C | – | | | | – | T | C | G | , | A | C | A | , | G | T | G | , | A | T | A | , | G | A | A | , | T | C | T | , | G | G | A | , | T | A | C | – | | – | A | G | C | , | T | G | T | , | C | A | C | , | T | A | T | , | C | T | T | , | A | G | A | , | C | C | T | , | A | T | G | – | | | | – | G | C | A | , | C | C | T | –3′ | | – | C | G | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCTAG-3′ and 5′-AGGTGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 980c_35ea
| 5′– | G | A | C | , | A | A | T | – | | 3′– | C | T | G | , | T | T | A | – | | | | – | T | A | T | , | C | C | G | , | T | G | C | , | T | G | A | , | T | A | G | , | T | C | C | , | C | A | T | , | T | G | G | – | | – | A | T | A | , | G | G | C | , | A | C | G | , | A | C | T | , | A | T | C | , | A | G | G | , | G | T | A | , | A | C | C | – | | | | – | T | T | C | , | C | A | G | –3′ | | – | A | A | G | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACAAT-3′ and 5′-CTGGAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 92f6_78e8
| 5′– | T | C | A | , | T | G | C | – | | 3′– | A | G | T | , | A | C | G | – | | | | – | T | T | A | , | G | T | C | , | T | C | C | , | T | G | T | , | A | C | T | , | G | T | A | , | G | G | A | , | T | A | C | – | | – | A | A | T | , | C | A | G | , | A | G | G | , | A | C | A | , | T | G | A | , | C | A | T | , | C | C | T | , | A | T | G | – | | | | – | T | C | A | , | C | T | G | –3′ | | – | A | G | T | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCATGC-3′ and 5′-CAGTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 9264_978d
| 5′– | A | G | T | , | A | T | C | – | | 3′– | T | C | A | , | T | A | G | – | | | | – | G | G | C | , | A | A | T | , | C | A | A | , | T | C | A | , | T | G | C | , | G | A | G | , | G | T | T | , | T | C | A | – | | – | C | C | G | , | T | T | A | , | G | T | T | , | A | G | T | , | A | C | G | , | C | T | C | , | C | A | A | , | A | G | T | – | | | | – | T | G | A | , | G | G | C | –3′ | | – | A | C | T | , | C | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTATC-3′ and 5′-GCCTCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 54da_1e2c
| 5′– | C | A | G | , | A | T | T | – | | 3′– | G | T | C | , | T | A | A | – | | | | – | G | A | T | , | A | C | A | , | G | G | G | , | T | T | C | , | A | G | C | , | A | T | G | , | T | A | A | , | G | C | A | – | | – | C | T | A | , | T | G | T | , | C | C | C | , | A | A | G | , | T | C | G | , | T | A | C | , | A | T | T | , | C | G | T | – | | | | – | G | T | A | , | C | T | T | –3′ | | – | C | A | T | , | G | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGATT-3′ and 5′-AAGTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 79e1_96d7
| 5′– | A | G | T | , | T | C | G | – | | 3′– | T | C | A | , | A | G | C | – | | | | – | A | C | T | , | G | C | C | , | A | C | T | , | C | G | A | , | G | A | G | , | T | C | T | , | G | A | C | , | T | T | C | – | | – | T | G | A | , | C | G | G | , | T | G | A | , | G | C | T | , | C | T | C | , | A | G | A | , | C | T | G | , | A | A | G | – | | | | – | A | T | C | , | T | A | C | –3′ | | – | T | A | G | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTTCG-3′ and 5′-GTAGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC d4fa_1dc1
| 5′– | G | C | T | , | T | C | A | – | | 3′– | C | G | A | , | A | G | T | – | | | | – | T | A | T | , | C | T | G | , | G | A | C | , | C | C | T | , | T | C | G | , | A | G | T | , | T | A | C | , | C | T | G | – | | – | A | T | A | , | G | A | C | , | C | T | G | , | G | G | A | , | A | G | C | , | T | C | A | , | A | T | G | , | G | A | C | – | | | | – | T | A | C | , | T | A | G | –3′ | | – | A | T | G | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTTCA-3′ and 5′-CTAGTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 0ebf_cc17
| 5′– | G | A | C | , | T | C | A | – | | 3′– | C | T | G | , | A | G | T | – | | | | – | T | G | A | , | C | A | A | , | G | C | T | , | T | C | T | , | C | T | A | , | A | C | C | , | T | G | A | , | A | C | G | – | | – | A | C | T | , | G | T | T | , | C | G | A | , | A | G | A | , | G | A | T | , | T | G | G | , | A | C | T | , | T | G | C | – | | | | – | C | C | T | , | A | G | T | –3′ | | – | G | G | A | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACTCA-3′ and 5′-ACTAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 53b6_4b64
| 5′– | G | C | A | , | G | T | T | – | | 3′– | C | G | T | , | C | A | A | – | | | | – | T | A | A | , | G | C | A | , | T | G | T | , | G | G | A | , | C | T | A | , | C | C | C | , | T | G | C | , | T | A | G | – | | – | A | T | T | , | C | G | T | , | A | C | A | , | C | C | T | , | G | A | T | , | G | G | G | , | A | C | G | , | A | T | C | – | | | | – | G | A | T | , | C | G | A | –3′ | | – | C | T | A | , | G | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCAGTT-3′ and 5′-TCGATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 81f4_c63a
| 5′– | T | C | A | , | A | G | G | – | | 3′– | A | G | T | , | T | C | C | – | | | | – | C | T | A | , | G | C | A | , | T | C | G | , | C | A | T | , | C | A | T | , | C | G | C | , | T | G | G | , | T | C | A | – | | – | G | A | T | , | C | G | T | , | A | G | C | , | G | T | A | , | G | T | A | , | G | C | G | , | A | C | C | , | A | G | T | – | | | | – | A | T | C | , | T | T | C | –3′ | | – | T | A | G | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAAGG-3′ and 5′-GAAGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 6c59_3bc6
| 5′– | G | A | C | , | C | G | T | – | | 3′– | C | T | G | , | G | C | A | – | | | | – | A | T | T | , | G | C | A | , | G | T | A | , | C | T | G | , | C | A | T | , | G | G | A | , | A | G | G | , | T | T | C | – | | – | T | A | A | , | C | G | T | , | C | A | T | , | G | A | C | , | G | T | A | , | C | C | T | , | T | C | C | , | A | A | G | – | | | | – | T | A | C | , | A | G | C | –3′ | | – | A | T | G | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACCGT-3′ and 5′-GCTGTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8b35_6b20
| 5′– | G | C | A | , | G | C | T | – | | 3′– | C | G | T | , | C | G | A | – | | | | – | A | T | G | , | C | G | A | , | C | C | A | , | T | A | G | , | C | A | T | , | G | T | T | , | G | A | T | , | G | A | C | – | | – | T | A | C | , | G | C | T | , | G | G | T | , | A | T | C | , | G | T | A | , | C | A | A | , | C | T | A | , | C | T | G | – | | | | – | T | T | C | , | A | C | G | –3′ | | – | A | A | G | , | T | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCAGCT-3′ and 5′-CGTGAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 654b_b3b0
| 5′– | G | T | C | , | A | A | T | – | | 3′– | C | A | G | , | T | T | A | – | | | | – | T | C | G | , | C | A | G | , | C | C | A | , | T | A | G | , | C | A | C | , | T | C | T | , | T | A | G | , | A | C | G | – | | – | A | G | C | , | G | T | C | , | G | G | T | , | A | T | C | , | G | T | G | , | A | G | A | , | A | T | C | , | T | G | C | – | | | | – | G | A | T | , | C | G | T | –3′ | | – | C | T | A | , | G | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCAAT-3′ and 5′-ACGATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC f49b_a1ee
| 5′– | T | G | A | , | T | G | G | – | | 3′– | A | C | T | , | A | C | C | – | | | | – | C | T | T | , | A | C | G | , | A | G | C | , | A | G | A | , | G | C | G | , | T | T | C | , | A | T | A | , | G | C | A | – | | – | G | A | A | , | T | G | C | , | T | C | G | , | T | C | T | , | C | G | C | , | A | A | G | , | T | A | T | , | C | G | T | – | | | | – | T | G | G | , | C | A | G | –3′ | | – | A | C | C | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGATGG-3′ and 5′-CTGCCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9061_71b0
| 5′– | T | G | A | , | G | A | C | – | | 3′– | A | C | T | , | C | T | G | – | | | | – | A | C | T | , | C | A | G | , | T | C | C | , | C | A | C | , | G | T | G | , | A | A | A | , | G | T | T | , | A | C | G | – | | – | T | G | A | , | G | T | C | , | A | G | G | , | G | T | G | , | C | A | C | , | T | T | T | , | C | A | A | , | T | G | C | – | | | | – | G | T | C | , | T | T | A | –3′ | | – | C | A | G | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAGAC-3′ and 5′-TAAGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 51d7_3bef
| 5′– | G | G | A | , | G | C | T | – | | 3′– | C | C | T | , | C | G | A | – | | | | – | C | T | T | , | A | G | T | , | T | G | A | , | C | G | T | , | G | A | C | , | G | G | T | , | C | A | T | , | G | G | T | – | | – | G | A | A | , | T | C | A | , | A | C | T | , | G | C | A | , | C | T | G | , | C | C | A | , | G | T | A | , | C | C | A | – | | | | – | T | T | C | , | T | A | C | –3′ | | – | A | A | G | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGAGCT-3′ and 5′-GTAGAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 352d_975b
| 5′– | T | A | C | , | T | C | G | – | | 3′– | A | T | G | , | A | G | C | – | | | | – | A | G | T | , | G | C | A | , | C | T | A | , | T | C | A | , | T | T | C | , | A | A | T | , | G | A | G | , | T | C | C | – | | – | T | C | A | , | C | G | T | , | G | A | T | , | A | G | T | , | A | A | G | , | T | T | A | , | C | T | C | , | A | G | G | – | | | | – | A | C | T | , | A | G | G | –3′ | | – | T | G | A | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TACTCG-3′ and 5′-CCTAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC bb29_5715
| 5′– | A | T | G | , | A | A | G | – | | 3′– | T | A | C | , | T | T | C | – | | | | – | G | A | C | , | T | A | T | , | G | A | G | , | A | T | C | , | A | C | C | , | C | T | A | , | A | G | C | , | T | G | T | – | | – | C | T | G | , | A | T | A | , | C | T | C | , | T | A | G | , | T | G | G | , | G | A | T | , | T | C | G | , | A | C | A | – | | | | – | G | A | C | , | A | T | T | –3′ | | – | C | T | G | , | T | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGAAG-3′ and 5′-AATGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC b7be_cabc
| 5′– | A | A | G | , | T | C | G | – | | 3′– | T | T | C | , | A | G | C | – | | | | – | G | C | A | , | T | A | C | , | T | G | T | , | G | A | A | , | T | G | A | , | A | C | G | , | C | T | G | , | C | A | T | – | | – | C | G | T | , | A | T | G | , | A | C | A | , | C | T | T | , | A | C | T | , | T | G | C | , | G | A | C | , | G | T | A | – | | | | – | C | G | T | , | A | C | T | –3′ | | – | G | C | A | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AAGTCG-3′ and 5′-AGTACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2802_0a29
| 5′– | C | C | G | , | A | A | T | – | | 3′– | G | G | C | , | T | T | A | – | | | | – | T | G | A | , | T | A | C | , | A | A | A | , | G | C | C | , | A | A | C | , | C | A | G | , | G | T | C | , | A | A | C | – | | – | A | C | T | , | A | T | G | , | T | T | T | , | C | G | G | , | T | T | G | , | G | T | C | , | C | A | G | , | T | T | G | – | | | | – | G | G | C | , | T | C | A | –3′ | | – | C | C | G | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCGAAT-3′ and 5′-TGAGCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1fbe_bc4c
| 5′– | C | T | G | , | A | C | T | – | | 3′– | G | A | C | , | T | G | A | – | | | | – | A | C | C | , | T | T | G | , | T | T | C | , | G | T | A | , | T | G | C | , | A | C | T | , | A | G | C | , | T | A | G | – | | – | T | G | G | , | A | A | C | , | A | A | G | , | C | A | T | , | A | C | G | , | T | G | A | , | T | C | G | , | A | T | C | – | | | | – | C | A | A | , | G | G | T | –3′ | | – | G | T | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGACT-3′ and 5′-ACCTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 028a_5634
| 5′– | T | A | C | , | A | G | G | – | | 3′– | A | T | G | , | T | C | C | – | | | | – | G | A | A | , | C | T | A | , | G | G | T | , | A | C | C | , | G | T | C | , | A | T | G | , | T | G | C | , | A | A | T | – | | – | C | T | T | , | G | A | T | , | C | C | A | , | T | G | G | , | C | A | G | , | T | A | C | , | A | C | G | , | T | T | A | – | | | | – | C | T | G | , | T | T | A | –3′ | | – | G | A | C | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TACAGG-3′ and 5′-TAACAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0c1b_a155
| 5′– | G | C | C | , | A | A | T | – | | 3′– | C | G | G | , | T | T | A | – | | | | – | A | T | C | , | C | T | G | , | T | A | G | , | A | A | C | , | C | T | C | , | G | A | C | , | A | A | C | , | T | G | C | – | | – | T | A | G | , | G | A | C | , | A | T | C | , | T | T | G | , | G | A | G | , | C | T | G | , | T | T | G | , | A | C | G | – | | | | – | T | G | A | , | A | A | G | –3′ | | – | A | C | T | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCCAAT-3′ and 5′-CTTTCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 18b4_da58
| 5′– | G | G | A | , | C | T | A | – | | 3′– | C | C | T | , | G | A | T | – | | | | – | C | T | G | , | A | T | T | , | G | C | A | , | G | T | G | , | A | T | G | , | T | G | G | , | C | A | T | , | G | T | A | – | | – | G | A | C | , | T | A | A | , | C | G | T | , | C | A | C | , | T | A | C | , | A | C | C | , | G | T | A | , | C | A | T | – | | | | – | C | T | T | , | T | C | A | –3′ | | – | G | A | A | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGACTA-3′ and 5′-TGAAAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 67d1_0114
| 5′– | T | G | G | , | A | G | C | – | | 3′– | A | C | C | , | T | C | G | – | | | | – | C | T | G | , | A | A | C | , | C | A | C | , | C | T | T | , | G | T | C | , | G | A | T | , | A | G | C | , | T | G | T | – | | – | G | A | C | , | T | T | G | , | G | T | G | , | G | A | A | , | C | A | G | , | C | T | A | , | T | C | G | , | A | C | A | – | | | | – | T | A | A | , | G | C | C | –3′ | | – | A | T | T | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGAGC-3′ and 5′-GGCTTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 32c8_32b0
| 5′– | A | C | T | , | A | A | C | – | | 3′– | T | G | A | , | T | T | G | – | | | | – | C | T | T | , | G | A | T | , | G | G | C | , | T | C | C | , | A | T | C | , | G | C | A | , | C | A | T | , | G | G | A | – | | – | G | A | A | , | C | T | A | , | C | C | G | , | A | G | G | , | T | A | G | , | C | G | T | , | G | T | A | , | C | C | T | – | | | | – | C | G | A | , | C | A | T | –3′ | | – | G | C | T | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTAAC-3′ and 5′-ATGTCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC dced_064a
| 5′– | G | T | C | , | A | C | T | – | | 3′– | C | A | G | , | T | G | A | – | | | | – | G | A | C | , | A | A | T | , | G | C | G | , | A | C | T | , | C | C | A | , | G | C | C | , | A | C | G | , | A | C | T | – | | – | C | T | G | , | T | T | A | , | C | G | C | , | T | G | A | , | G | G | T | , | C | G | G | , | T | G | C | , | T | G | A | – | | | | – | G | T | A | , | G | C | A | –3′ | | – | C | A | T | , | C | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCACT-3′ and 5′-TGCTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 3bb8_dd61
| 5′– | C | G | T | , | C | T | A | – | | 3′– | G | C | A | , | G | A | T | – | | | | – | C | A | C | , | C | G | T | , | T | G | C | , | G | T | G | , | G | A | C | , | T | G | C | , | G | A | C | , | A | G | T | – | | – | G | T | G | , | G | C | A | , | A | C | G | , | C | A | C | , | C | T | G | , | A | C | G | , | C | T | G | , | T | C | A | – | | | | – | G | A | T | , | G | T | T | –3′ | | – | C | T | A | , | C | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTCTA-3′ and 5′-AACATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1d49_bb41
| 5′– | G | G | C | , | T | T | A | – | | 3′– | C | C | G | , | A | A | T | – | | | | – | A | A | G | , | C | C | T | , | T | C | C | , | A | C | A | , | T | G | T | , | G | C | T | , | G | T | A | , | T | C | G | – | | – | T | T | C | , | G | G | A | , | A | G | G | , | T | G | T | , | A | C | A | , | C | G | A | , | C | A | T | , | A | G | C | – | | | | – | A | G | T | , | A | T | G | –3′ | | – | T | C | A | , | T | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGCTTA-3′ and 5′-CATACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 0ac7_fa1a
| 5′– | T | A | A | , | C | T | G | – | | 3′– | A | T | T | , | G | A | C | – | | | | – | G | C | A | , | C | G | T | , | A | A | G | , | T | T | T | , | C | C | A | , | A | A | G | , | A | T | A | , | G | C | A | – | | – | C | G | T | , | G | C | A | , | T | T | C | , | A | A | A | , | G | G | T | , | T | T | C | , | T | A | T | , | C | G | T | – | | | | – | G | G | A | , | G | A | T | –3′ | | – | C | C | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAACTG-3′ and 5′-ATCTCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC a003_3f6e
| 5′– | C | T | T | , | T | G | A | – | | 3′– | G | A | A | , | A | C | T | – | | | | – | A | G | C | , | T | G | C | , | A | G | A | , | G | C | T | , | A | C | C | , | C | T | A | , | C | C | T | , | C | A | G | – | | – | T | C | G | , | A | C | G | , | T | C | T | , | C | G | A | , | T | G | G | , | G | A | T | , | G | G | A | , | G | T | C | – | | | | – | G | T | C | , | A | T | T | –3′ | | – | C | A | G | , | T | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTTTGA-3′ and 5′-AATGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8cce_0743
| 5′– | T | T | C | , | A | G | C | – | | 3′– | A | A | G | , | T | C | G | – | | | | – | G | T | A | , | C | G | C | , | A | G | A | , | A | G | A | , | A | C | A | , | C | G | C | , | T | A | G | , | C | G | T | – | | – | C | A | T | , | G | C | G | , | T | C | T | , | T | C | T | , | T | G | T | , | G | C | G | , | A | T | C | , | G | C | A | – | | | | – | C | A | A | , | T | C | A | –3′ | | – | G | T | T | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCAGC-3′ and 5′-TGATTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 7880_3b5d
| 5′– | G | T | C | , | C | A | A | – | | 3′– | C | A | G | , | G | T | T | – | | | | – | G | T | C | , | T | G | A | , | C | C | T | , | G | A | G | , | A | T | C | , | T | G | C | , | T | C | T | , | G | T | A | – | | – | C | A | G | , | A | C | T | , | G | G | A | , | C | T | C | , | T | A | G | , | A | C | G | , | A | G | A | , | C | A | T | – | | | | – | C | A | G | , | C | A | T | –3′ | | – | G | T | C | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCCAA-3′ and 5′-ATGCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2499_e2a2
| 5′– | C | A | A | , | G | T | A | – | | 3′– | G | T | T | , | C | A | T | – | | | | – | T | G | A | , | A | T | C | , | A | T | C | , | C | T | G | , | T | A | A | , | C | A | A | , | G | C | T | , | G | A | G | – | | – | A | C | T | , | T | A | G | , | T | A | G | , | G | A | C | , | A | T | T | , | G | T | T | , | C | G | A | , | C | T | C | – | | | | – | T | C | A | , | T | C | C | –3′ | | – | A | G | T | , | A | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAAGTA-3′ and 5′-GGATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC ca70_a788
| 5′– | G | A | T | , | T | G | A | – | | 3′– | C | T | A | , | A | C | T | – | | | | – | C | A | G | , | G | A | T | , | A | C | A | , | T | A | G | , | T | C | C | , | G | A | T | , | C | G | T | , | C | C | A | – | | – | G | T | C | , | C | T | A | , | T | G | T | , | A | T | C | , | A | G | G | , | C | T | A | , | G | C | A | , | G | G | T | – | | | | – | T | A | A | , | G | C | C | –3′ | | – | A | T | T | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATTGA-3′ and 5′-GGCTTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC eb2e_1ef8
| 5′– | C | C | A | , | C | C | T | – | | 3′– | G | G | T | , | G | G | A | – | | | | – | G | C | A | , | A | C | T | , | G | C | C | , | T | C | C | , | T | C | T | , | C | T | C | , | T | C | T | , | G | A | A | – | | – | C | G | T | , | T | G | A | , | C | G | G | , | A | G | G | , | A | G | A | , | G | A | G | , | A | G | A | , | C | T | T | – | | | | – | C | C | A | , | T | C | A | –3′ | | – | G | G | T | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCACCT-3′ and 5′-TGATGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3ad8_f5d5
| 5′– | C | G | T | , | G | G | A | – | | 3′– | G | C | A | , | C | C | T | – | | | | – | T | G | A | , | A | T | C | , | G | T | C | , | A | C | A | , | A | C | C | , | T | G | C | , | T | A | G | , | C | A | C | – | | – | A | C | T | , | T | A | G | , | C | A | G | , | T | G | T | , | T | G | G | , | A | C | G | , | A | T | C | , | G | T | G | – | | | | – | G | G | T | , | T | C | A | –3′ | | – | C | C | A | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTGGA-3′ and 5′-TGAACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 38eb_f5b6
| 5′– | A | T | T | , | C | A | G | – | | 3′– | T | A | A | , | G | T | C | – | | | | – | T | T | C | , | G | G | A | , | A | T | G | , | T | G | T | , | G | G | A | , | A | C | T | , | G | T | A | , | C | A | G | – | | – | A | A | G | , | C | C | T | , | T | A | C | , | A | C | A | , | C | C | T | , | T | G | A | , | C | A | T | , | G | T | C | – | | | | – | T | T | G | , | A | T | C | –3′ | | – | A | A | C | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATTCAG-3′ and 5′-GATCAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 2982_5f9b
| 5′– | G | A | A | , | T | G | A | – | | 3′– | C | T | T | , | A | C | T | – | | | | – | T | A | A | , | C | G | T | , | C | A | C | , | G | G | A | , | A | G | G | , | G | A | C | , | C | T | A | , | C | T | G | – | | – | A | T | T | , | G | C | A | , | G | T | G | , | C | C | T | , | T | C | C | , | C | T | G | , | G | A | T | , | G | A | C | – | | | | – | T | C | A | , | A | G | G | –3′ | | – | A | G | T | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GAATGA-3′ and 5′-CCTTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7efe_1d1a
| 5′– | C | T | G | , | T | C | A | – | | 3′– | G | A | C | , | A | G | T | – | | | | – | G | C | T | , | T | G | A | , | C | T | C | , | A | C | G | , | A | G | A | , | T | G | G | , | C | A | T | , | G | G | A | – | | – | C | G | A | , | A | C | T | , | G | A | G | , | T | G | C | , | T | C | T | , | A | C | C | , | G | T | A | , | C | C | T | – | | | | – | G | G | C | , | T | C | A | –3′ | | – | C | C | G | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGTCA-3′ and 5′-TGAGCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7fdb_ec7a
| 5′– | A | A | C | , | T | C | C | – | | 3′– | T | T | G | , | A | G | G | – | | | | – | G | G | C | , | T | A | T | , | G | T | T | , | G | G | T | , | T | C | C | , | G | A | A | , | G | C | C | , | T | A | A | – | | – | C | C | G | , | A | T | A | , | C | A | A | , | C | C | A | , | A | G | G | , | C | T | T | , | C | G | G | , | A | T | T | – | | | | – | G | G | T | , | C | T | A | –3′ | | – | C | C | A | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACTCC-3′ and 5′-TAGACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a63d_9fb3
| 5′– | G | G | A | , | T | G | A | – | | 3′– | C | C | T | , | A | C | T | – | | | | – | C | A | G | , | T | G | C | , | C | A | T | , | A | C | A | , | A | G | A | , | C | G | C | , | A | C | G | , | A | A | T | – | | – | G | T | C | , | A | C | G | , | G | T | A | , | T | G | T | , | T | C | T | , | G | C | G | , | T | G | C | , | T | T | A | – | | | | – | A | G | G | , | T | G | C | –3′ | | – | T | C | C | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGATGA-3′ and 5′-GCACCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 857d_9f1f
| 5′– | T | C | A | , | A | T | C | – | | 3′– | A | G | T | , | T | A | G | – | | | | – | T | G | C | , | A | T | G | , | T | A | C | , | C | T | C | , | C | A | C | , | G | T | A | , | C | C | T | , | A | A | G | – | | – | A | C | G | , | T | A | C | , | A | T | G | , | G | A | G | , | G | T | G | , | C | A | T | , | G | G | A | , | T | T | C | – | | | | – | G | C | A | , | A | C | T | –3′ | | – | C | G | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAATC-3′ and 5′-AGTTGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC c04c_0744
| 5′– | C | C | T | , | G | C | A | – | | 3′– | G | G | A | , | C | G | T | – | | | | – | C | C | T | , | G | A | C | , | G | T | C | , | G | T | T | , | A | G | T | , | G | T | C | , | T | G | T | , | C | G | A | – | | – | G | G | A | , | C | T | G | , | C | A | G | , | C | A | A | , | T | C | A | , | C | A | G | , | A | C | A | , | G | C | T | – | | | | – | C | A | G | , | T | G | A | –3′ | | – | G | T | C | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTGCA-3′ and 5′-TCACTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 0b3a_2577
| 5′– | A | C | T | , | A | C | G | – | | 3′– | T | G | A | , | T | G | C | – | | | | – | C | T | T | , | A | G | T | , | T | T | C | , | A | C | G | , | A | G | A | , | A | T | C | , | C | C | A | , | G | C | T | – | | – | G | A | A | , | T | C | A | , | A | A | G | , | T | G | C | , | T | C | T | , | T | A | G | , | G | G | T | , | C | G | A | – | | | | – | A | C | T | , | A | G | C | –3′ | | – | T | G | A | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTACG-3′ and 5′-GCTAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 50a1_06cd
| 5′– | C | T | A | , | C | T | T | – | | 3′– | G | A | T | , | G | A | A | – | | | | – | G | A | A | , | A | C | T | , | A | A | G | , | C | T | T | , | C | C | T | , | G | C | C | , | A | C | A | , | G | G | T | – | | – | C | T | T | , | T | G | A | , | T | T | C | , | G | A | A | , | G | G | A | , | C | G | G | , | T | G | T | , | C | C | A | – | | | | – | A | T | C | , | T | G | G | –3′ | | – | T | A | G | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTACTT-3′ and 5′-CCAGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 8ec0_a8c5
| 5′– | T | G | A | , | T | A | G | – | | 3′– | A | C | T | , | A | T | C | – | | | | – | A | T | C | , | G | A | G | , | A | G | T | , | A | C | A | , | C | T | C | , | T | T | G | , | A | G | C | , | T | G | G | – | | – | T | A | G | , | C | T | C | , | T | C | A | , | T | G | T | , | G | A | G | , | A | A | C | , | T | C | G | , | A | C | C | – | | | | – | T | C | A | , | A | G | G | –3′ | | – | A | G | T | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGATAG-3′ and 5′-CCTTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2b3d_f2f9
| 5′– | G | T | C | , | C | C | A | – | | 3′– | C | A | G | , | G | G | T | – | | | | – | G | A | G | , | T | T | C | , | G | C | A | , | A | G | G | , | G | T | A | , | G | A | C | , | G | A | G | , | C | T | A | – | | – | C | T | C | , | A | A | G | , | C | G | T | , | T | C | C | , | C | A | T | , | C | T | G | , | C | T | C | , | G | A | T | – | | | | – | A | T | C | , | T | G | C | –3′ | | – | T | A | G | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCCCA-3′ and 5′-GCAGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 53cc_a8c9
| 5′– | C | A | A | , | A | G | T | – | | 3′– | G | T | T | , | T | C | A | – | | | | – | T | G | C | , | A | T | T | , | C | T | C | , | T | C | A | , | T | G | T | , | G | C | T | , | A | C | G | , | T | G | G | – | | – | A | C | G | , | T | A | A | , | G | A | G | , | A | G | T | , | A | C | A | , | C | G | A | , | T | G | C | , | A | C | C | – | | | | – | T | G | G | , | T | A | C | –3′ | | – | A | C | C | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAAAGT-3′ and 5′-GTACCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4f9a_48a4
| 5′– | A | A | T | , | C | A | G | – | | 3′– | T | T | A | , | G | T | C | – | | | | – | C | A | G | , | G | T | A | , | T | C | A | , | T | G | T | , | C | C | C | , | A | C | T | , | C | C | G | , | A | T | A | – | | – | G | T | C | , | C | A | T | , | A | G | T | , | A | C | A | , | G | G | G | , | T | G | A | , | G | G | C | , | T | A | T | – | | | | – | G | G | A | , | G | A | T | –3′ | | – | C | C | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AATCAG-3′ and 5′-ATCTCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5718_c43b
| 5′– | T | T | C | , | T | A | C | – | | 3′– | A | A | G | , | A | T | G | – | | | | – | T | G | A | , | C | G | C | , | A | C | C | , | C | A | A | , | C | G | T | , | G | G | T | , | A | G | T | , | A | C | C | – | | – | A | C | T | , | G | C | G | , | T | G | G | , | G | T | T | , | G | C | A | , | C | C | A | , | T | C | A | , | T | G | G | – | | | | – | G | T | A | , | G | A | A | –3′ | | – | C | A | T | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCTAC-3′ and 5′-TTCTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 12b6_a0ae
| 5′– | C | A | T | , | G | C | A | – | | 3′– | G | T | A | , | C | G | T | – | | | | – | T | G | A | , | C | T | G | , | A | C | C | , | C | T | C | , | G | A | C | , | G | T | A | , | T | G | A | , | A | G | C | – | | – | A | C | T | , | G | A | C | , | T | G | G | , | G | A | G | , | C | T | G | , | C | A | T | , | A | C | T | , | T | C | G | – | | | | – | G | G | A | , | G | A | T | –3′ | | – | C | C | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATGCA-3′ and 5′-ATCTCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 9443_187c
| 5′– | C | G | T | , | G | A | A | – | | 3′– | G | C | A | , | C | T | T | – | | | | – | A | G | C | , | T | G | C | , | A | A | A | , | C | C | A | , | C | G | C | , | T | G | G | , | T | C | G | , | A | A | C | – | | – | T | C | G | , | A | C | G | , | T | T | T | , | G | G | T | , | G | C | G | , | A | C | C | , | A | G | C | , | T | T | G | – | | | | – | G | C | A | , | A | G | T | –3′ | | – | C | G | T | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTGAA-3′ and 5′-ACTTGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3e48_5ed5
| 5′– | C | C | G | , | A | A | T | – | | 3′– | G | G | C | , | T | T | A | – | | | | – | G | A | T | , | A | G | C | , | A | A | G | , | A | G | A | , | G | T | T | , | C | T | G | , | A | C | C | , | G | T | T | – | | – | C | T | A | , | T | C | G | , | T | T | C | , | T | C | T | , | C | A | A | , | G | A | C | , | T | G | G | , | C | A | A | – | | | | – | G | T | A | , | G | C | A | –3′ | | – | C | A | T | , | C | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCGAAT-3′ and 5′-TGCTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3e88_d63c
| 5′– | C | T | A | , | G | T | A | – | | 3′– | G | A | T | , | C | A | T | – | | | | – | T | T | A | , | C | G | T | , | A | C | A | , | C | T | A | , | C | T | T | , | T | C | C | , | T | C | G | , | C | A | G | – | | – | A | A | T | , | G | C | A | , | T | G | T | , | G | A | T | , | G | A | A | , | A | G | G | , | A | G | C | , | G | T | C | – | | | | – | T | A | G | , | A | G | C | –3′ | | – | A | T | C | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTAGTA-3′ and 5′-GCTCTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6157_2aa6
| 5′– | G | T | T | , | G | T | A | – | | 3′– | C | A | A | , | C | A | T | – | | | | – | C | G | A | , | C | G | T | , | C | A | T | , | C | C | A | , | C | A | G | , | G | T | G | , | A | T | A | , | G | C | A | – | | – | G | C | T | , | G | C | A | , | G | T | A | , | G | G | T | , | G | T | C | , | C | A | C | , | T | A | T | , | C | G | T | – | | | | – | G | T | T | , | G | G | A | –3′ | | – | C | A | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTGTA-3′ and 5′-TCCAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC de85_0cd0
| 5′– | T | G | A | , | G | C | C | – | | 3′– | A | C | T | , | C | G | G | – | | | | – | A | G | A | , | C | A | T | , | G | C | G | , | T | A | C | , | G | T | G | , | A | A | A | , | G | A | G | , | A | T | C | – | | – | T | C | T | , | G | T | A | , | C | G | C | , | A | T | G | , | C | A | C | , | T | T | T | , | C | T | C | , | T | A | G | – | | | | – | A | C | T | , | A | A | C | –3′ | | – | T | G | A | , | T | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAGCC-3′ and 5′-GTTAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b6aa_83e2
| 5′– | G | A | C | , | A | A | T | – | | 3′– | C | T | G | , | T | T | A | – | | | | – | A | G | T | , | C | C | C | , | A | C | C | , | A | T | A | , | C | G | A | , | C | G | A | , | T | A | G | , | A | T | C | – | | – | T | C | A | , | G | G | G | , | T | G | G | , | T | A | T | , | G | C | T | , | G | C | T | , | A | T | C | , | T | A | G | – | | | | – | G | A | A | , | T | G | A | –3′ | | – | C | T | T | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACAAT-3′ and 5′-TCATTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a83d_8281
| 5′– | G | G | A | , | G | G | T | – | | 3′– | C | C | T | , | C | C | A | – | | | | – | C | G | A | , | A | G | T | , | G | A | C | , | C | C | A | , | T | C | C | , | A | G | G | , | T | G | A | , | C | C | A | – | | – | G | C | T | , | T | C | A | , | C | T | G | , | G | G | T | , | A | G | G | , | T | C | C | , | A | C | T | , | G | G | T | – | | | | – | G | G | A | , | T | G | A | –3′ | | – | C | C | T | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGAGGT-3′ and 5′-TCATCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2fd1_0854
| 5′– | G | C | T | , | A | G | T | – | | 3′– | C | G | A | , | T | C | A | – | | | | – | T | T | G | , | A | C | C | , | A | C | A | , | G | G | A | , | A | C | G | , | G | A | A | , | C | G | C | , | T | A | C | – | | – | A | A | C | , | T | G | G | , | T | G | T | , | C | C | T | , | T | G | C | , | C | T | T | , | G | C | G | , | A | T | G | – | | | | – | G | A | A | , | C | T | A | –3′ | | – | C | T | T | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTAGT-3′ and 5′-TAGTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 2c24_da82
| 5′– | C | G | T | , | G | G | A | – | | 3′– | G | C | A | , | C | C | T | – | | | | – | G | G | A | , | G | C | T | , | G | A | T | , | G | A | A | , | A | C | C | , | T | G | T | , | T | T | G | , | A | C | A | – | | – | C | C | T | , | C | G | A | , | C | T | A | , | C | T | T | , | T | G | G | , | A | C | A | , | A | A | C | , | T | G | T | – | | | | – | A | C | G | , | T | T | C | –3′ | | – | T | G | C | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTGGA-3′ and 5′-GAACGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c93c_63c1
| 5′– | T | G | G | , | A | C | G | – | | 3′– | A | C | C | , | T | G | C | – | | | | – | G | T | G | , | C | C | A | , | C | C | A | , | A | T | G | , | A | A | G | , | G | A | A | , | G | A | C | , | A | C | T | – | | – | C | A | C | , | G | G | T | , | G | G | T | , | T | A | C | , | T | T | C | , | C | T | T | , | C | T | G | , | T | G | A | – | | | | – | G | G | A | , | C | T | A | –3′ | | – | C | C | T | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGACG-3′ and 5′-TAGTCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2f5e_9345
| 5′– | C | G | A | , | T | C | A | – | | 3′– | G | C | T | , | A | G | T | – | | | | – | T | G | A | , | T | T | C | , | T | C | C | , | T | G | G | , | A | T | C | , | T | G | G | , | T | C | A | , | C | C | G | – | | – | A | C | T | , | A | A | G | , | A | G | G | , | A | C | C | , | T | A | G | , | A | C | C | , | A | G | T | , | G | G | C | – | | | | – | T | T | C | , | A | C | C | –3′ | | – | A | A | G | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGATCA-3′ and 5′-GGTGAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC f0f6_df28
| 5′– | T | G | A | , | C | C | G | – | | 3′– | A | C | T | , | G | G | C | – | | | | – | C | A | T | , | C | G | A | , | C | C | C | , | A | A | T | , | G | C | T | , | G | T | C | , | T | A | C | , | T | G | T | – | | – | G | T | A | , | G | C | T | , | G | G | G | , | T | T | A | , | C | G | A | , | C | A | G | , | A | T | G | , | A | C | A | – | | | | – | A | A | T | , | C | A | G | –3′ | | – | T | T | A | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGACCG-3′ and 5′-CTGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC f338_9f8a
| 5′– | A | G | C | , | C | T | G | – | | 3′– | T | C | G | , | G | A | C | – | | | | – | G | C | A | , | A | C | T | , | C | G | A | , | T | T | G | , | A | A | G | , | C | T | T | , | C | C | A | , | G | T | A | – | | – | C | G | T | , | T | G | A | , | G | C | T | , | A | A | C | , | T | T | C | , | G | A | A | , | G | G | T | , | C | A | T | – | | | | – | A | T | T | , | C | T | G | –3′ | | – | T | A | A | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGCCTG-3′ and 5′-CAGAAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC d2db_3edf
| 5′– | C | T | G | , | C | A | A | – | | 3′– | G | A | C | , | G | T | T | – | | | | – | G | A | C | , | G | T | A | , | T | G | T | , | T | C | C | , | T | C | T | , | A | C | A | , | C | C | A | , | G | C | T | – | | – | C | T | G | , | C | A | T | , | A | C | A | , | A | G | G | , | A | G | A | , | T | G | T | , | G | G | T | , | C | G | A | – | | | | – | A | G | T | , | A | C | G | –3′ | | – | T | C | A | , | T | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGCAA-3′ and 5′-CGTACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC edd8_0ce8
| 5′– | A | T | T | , | C | T | G | – | | 3′– | T | A | A | , | G | A | C | – | | | | – | C | G | T | , | C | C | A | , | C | C | C | , | T | C | A | , | C | G | G | , | A | T | C | , | G | A | T | , | A | C | T | – | | – | G | C | A | , | G | G | T | , | G | G | G | , | A | G | T | , | G | C | C | , | T | A | G | , | C | T | A | , | T | G | A | – | | | | – | C | A | G | , | G | T | A | –3′ | | – | G | T | C | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATTCTG-3′ and 5′-TACCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 429d_b04f
| 5′– | G | A | C | , | G | G | T | – | | 3′– | C | T | G | , | C | C | A | – | | | | – | C | G | T | , | C | T | A | , | G | G | T | , | G | A | C | , | A | T | G | , | G | C | A | , | G | G | C | , | A | A | T | – | | – | G | C | A | , | G | A | T | , | C | C | A | , | C | T | G | , | T | A | C | , | C | G | T | , | C | C | G | , | T | T | A | – | | | | – | G | C | T | , | C | C | A | –3′ | | – | C | G | A | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACGGT-3′ and 5′-TGGAGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 6089_75de
| 5′– | G | T | C | , | G | A | T | – | | 3′– | C | A | G | , | C | T | A | – | | | | – | A | G | T | , | T | A | C | , | T | A | T | , | G | A | C | , | G | T | C | , | T | C | C | , | T | A | C | , | G | T | G | – | | – | T | C | A | , | A | T | G | , | A | T | A | , | C | T | G | , | C | A | G | , | A | G | G | , | A | T | G | , | C | A | C | – | | | | – | G | T | C | , | C | C | A | –3′ | | – | C | A | G | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCGAT-3′ and 5′-TGGGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 7b22_369a
| 5′– | A | G | G | , | T | T | G | – | | 3′– | T | C | C | , | A | A | C | – | | | | – | T | G | A | , | G | C | A | , | C | C | C | , | A | A | G | , | G | C | A | , | C | A | G | , | A | C | G | , | A | T | G | – | | – | A | C | T | , | C | G | T | , | G | G | G | , | T | T | C | , | C | G | T | , | G | T | C | , | T | G | C | , | T | A | C | – | | | | – | T | C | C | , | A | A | C | –3′ | | – | A | G | G | , | T | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTTG-3′ and 5′-GTTGGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC ca51_66da
| 5′– | T | G | C | , | C | A | G | – | | 3′– | A | C | G | , | G | T | C | – | | | | – | G | C | T | , | C | G | A | , | G | T | G | , | C | T | A | , | C | G | G | , | A | T | T | , | C | G | A | , | C | T | A | – | | – | C | G | A | , | G | C | T | , | C | A | C | , | G | A | T | , | G | C | C | , | T | A | A | , | G | C | T | , | G | A | T | – | | | | – | T | C | C | , | T | A | C | –3′ | | – | A | G | G | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCCAG-3′ and 5′-GTAGGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC d441_543c
| 5′– | A | C | G | , | T | A | G | – | | 3′– | T | G | C | , | A | T | C | – | | | | – | G | G | T | , | A | A | C | , | T | G | C | , | A | A | C | , | T | C | T | , | T | C | C | , | A | G | T | , | G | C | A | – | | – | C | C | A | , | T | T | G | , | A | C | G | , | T | T | G | , | A | G | A | , | A | G | G | , | T | C | A | , | C | G | T | – | | | | – | A | T | G | , | A | G | C | –3′ | | – | T | A | C | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACGTAG-3′ and 5′-GCTCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC afab_15bc
| 5′– | G | T | T | , | A | G | T | – | | 3′– | C | A | A | , | T | C | A | – | | | | – | C | G | C | , | A | A | T | , | C | C | T | , | T | G | C | , | A | A | G | , | T | G | G | , | A | T | C | , | G | G | T | – | | – | G | C | G | , | T | T | A | , | G | G | A | , | A | C | G | , | T | T | C | , | A | C | C | , | T | A | G | , | C | C | A | – | | | | – | G | T | T | , | C | T | A | –3′ | | – | C | A | A | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTAGT-3′ and 5′-TAGAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 0ae7_e7c7
| 5′– | T | C | C | , | T | A | C | – | | 3′– | A | G | G | , | A | T | G | – | | | | – | C | G | T | , | G | T | A | , | A | G | T | , | C | T | G | , | C | T | T | , | G | C | C | , | T | C | A | , | G | T | T | – | | – | G | C | A | , | C | A | T | , | T | C | A | , | G | A | C | , | G | A | A | , | C | G | G | , | A | G | T | , | C | A | A | – | | | | – | A | T | C | , | A | A | C | –3′ | | – | T | A | G | , | T | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCCTAC-3′ and 5′-GTTGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5bb8_fb50
| 5′– | G | C | A | , | G | T | A | – | | 3′– | C | G | T | , | C | A | T | – | | | | – | A | T | C | , | C | G | A | , | T | A | C | , | A | C | A | , | A | T | C | , | G | A | C | , | T | C | A | , | C | G | G | – | | – | T | A | G | , | G | C | T | , | A | T | G | , | T | G | T | , | T | A | G | , | C | T | G | , | A | G | T | , | G | C | C | – | | | | – | A | A | T | , | G | C | C | –3′ | | – | T | T | A | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCAGTA-3′ and 5′-GGCATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC cf7a_168d
| 5′– | G | C | C | , | A | G | T | – | | 3′– | C | G | G | , | T | C | A | – | | | | – | T | G | C | , | T | G | A | , | C | C | A | , | G | T | A | , | G | G | A | , | G | A | G | , | T | C | T | , | A | C | G | – | | – | A | C | G | , | A | C | T | , | G | G | T | , | C | A | T | , | C | C | T | , | C | T | C | , | A | G | A | , | T | G | C | – | | | | – | C | T | G | , | T | C | A | –3′ | | – | G | A | C | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCCAGT-3′ and 5′-TGACAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC a983_cec7
| 5′– | G | T | A | , | C | C | T | – | | 3′– | C | A | T | , | G | G | A | – | | | | – | C | G | G | , | T | A | T | , | G | A | A | , | A | C | T | , | A | C | A | , | T | C | G | , | A | G | C | , | C | T | T | – | | – | G | C | C | , | A | T | A | , | C | T | T | , | T | G | A | , | T | G | T | , | A | G | C | , | T | C | G | , | G | A | A | – | | | | – | A | T | C | , | T | T | G | –3′ | | – | T | A | G | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTACCT-3′ and 5′-CAAGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 0470_33ea
| 5′– | G | A | T | , | T | G | A | – | | 3′– | C | T | A | , | A | C | T | – | | | | – | T | C | A | , | T | G | C | , | C | A | C | , | C | A | T | , | A | G | G | , | A | G | C | , | T | C | T | , | T | A | G | – | | – | A | G | T | , | A | C | G | , | G | T | G | , | G | T | A | , | T | C | C | , | T | C | G | , | A | G | A | , | A | T | C | – | | | | – | A | G | T | , | A | G | G | –3′ | | – | T | C | A | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATTGA-3′ and 5′-CCTACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 9c72_1527
| 5′– | G | T | A | , | G | T | T | – | | 3′– | C | A | T | , | C | A | A | – | | | | – | T | A | T | , | C | G | T | , | A | G | G | , | G | A | A | , | G | A | A | , | G | C | A | , | T | G | C | , | A | G | G | – | | – | A | T | A | , | G | C | A | , | T | C | C | , | C | T | T | , | C | T | T | , | C | G | T | , | A | C | G | , | T | C | C | – | | | | – | C | C | G | , | T | C | A | –3′ | | – | G | G | C | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTAGTT-3′ and 5′-TGACGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC f7f6_a73a
| 5′– | T | C | C | , | A | C | C | – | | 3′– | A | G | G | , | T | G | G | – | | | | – | A | A | C | , | T | G | C | , | C | T | A | , | C | G | C | , | T | A | A | , | G | C | A | , | G | T | A | , | C | T | G | – | | – | T | T | G | , | A | C | G | , | G | A | T | , | G | C | G | , | A | T | T | , | C | G | T | , | C | A | T | , | G | A | C | – | | | | – | A | T | G | , | T | G | C | –3′ | | – | T | A | C | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCCACC-3′ and 5′-GCACAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 6bea_b328
| 5′– | A | A | G | , | T | G | G | – | | 3′– | T | T | C | , | A | C | C | – | | | | – | A | T | G | , | A | C | A | , | A | G | T | , | G | G | T | , | T | G | C | , | T | G | C | , | A | A | C | , | T | C | G | – | | – | T | A | C | , | T | G | T | , | T | C | A | , | C | C | A | , | A | C | G | , | A | C | G | , | T | T | G | , | A | G | C | – | | | | – | T | C | A | , | T | A | G | –3′ | | – | A | G | T | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AAGTGG-3′ and 5′-CTATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 26f8_ef4d
| 5′– | G | G | A | , | A | G | T | – | | 3′– | C | C | T | , | T | C | A | – | | | | – | C | A | C | , | T | G | A | , | G | A | A | , | A | C | A | , | C | T | C | , | G | A | C | , | G | G | T | , | C | A | T | – | | – | G | T | G | , | A | C | T | , | C | T | T | , | T | G | T | , | G | A | G | , | C | T | G | , | C | C | A | , | G | T | A | – | | | | – | T | T | C | , | G | A | C | –3′ | | – | A | A | G | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGAAGT-3′ and 5′-GTCGAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 59bf_3482
| 5′– | A | G | T | , | T | A | C | – | | 3′– | T | C | A | , | A | T | G | – | | | | – | T | C | C | , | G | A | C | , | T | T | A | , | C | T | T | , | G | C | T | , | T | G | C | , | T | C | A | , | G | G | C | – | | – | A | G | G | , | C | T | G | , | A | A | T | , | G | A | A | , | C | G | A | , | A | C | G | , | A | G | T | , | C | C | G | – | | | | – | G | A | T | , | C | A | T | –3′ | | – | C | T | A | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTTAC-3′ and 5′-ATGATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0171_f707
| 5′– | T | G | G | , | A | C | G | – | | 3′– | A | C | C | , | T | G | C | – | | | | – | A | C | A | , | C | T | G | , | A | T | T | , | C | C | A | , | C | A | A | , | C | T | C | , | A | C | T | , | G | A | C | – | | – | T | G | T | , | G | A | C | , | T | A | A | , | G | G | T | , | G | T | T | , | G | A | G | , | T | G | A | , | C | T | G | – | | | | – | C | T | T | , | C | A | A | –3′ | | – | G | A | A | , | G | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGACG-3′ and 5′-TTGAAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 5d0d_2ff1
| 5′– | C | T | A | , | A | G | T | – | | 3′– | G | A | T | , | T | C | A | – | | | | – | A | G | T | , | C | T | C | , | C | A | G | , | T | G | C | , | G | A | C | , | A | A | G | , | T | C | A | , | T | A | G | – | | – | T | C | A | , | G | A | G | , | G | T | C | , | A | C | G | , | C | T | G | , | T | T | C | , | A | G | T | , | A | T | C | – | | | | – | G | C | A | , | G | T | T | –3′ | | – | C | G | T | , | C | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTAAGT-3′ and 5′-AACTGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0d65_4db3
| 5′– | A | G | G | , | T | A | C | – | | 3′– | T | C | C | , | A | T | G | – | | | | – | A | T | G | , | C | T | C | , | T | G | G | , | T | T | C | , | A | T | G | , | G | T | T | , | A | G | C | , | G | T | C | – | | – | T | A | C | , | G | A | G | , | A | C | C | , | A | A | G | , | T | A | C | , | C | A | A | , | T | C | G | , | C | A | G | – | | | | – | C | T | T | , | A | G | T | –3′ | | – | G | A | A | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTAC-3′ and 5′-ACTAAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 1106_3e62
| 5′– | T | C | A | , | T | C | C | – | | 3′– | A | G | T | , | A | G | G | – | | | | – | C | C | A | , | A | G | T | , | G | C | A | , | A | G | C | , | T | A | C | , | A | A | G | , | C | C | A | , | G | T | T | – | | – | G | G | T | , | T | C | A | , | C | G | T | , | T | C | G | , | A | T | G | , | T | T | C | , | G | G | T | , | C | A | A | – | | | | – | C | A | T | , | A | G | T | –3′ | | – | G | T | A | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCATCC-3′ and 5′-ACTATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC bba9_c33b
| 5′– | C | T | A | , | C | T | T | – | | 3′– | G | A | T | , | G | A | A | – | | | | – | A | G | C | , | A | C | T | , | T | C | C | , | T | C | C | , | A | T | C | , | A | A | G | , | C | A | T | , | T | G | C | – | | – | T | C | G | , | T | G | A | , | A | G | G | , | A | G | G | , | T | A | G | , | T | T | C | , | G | T | A | , | A | C | G | – | | | | – | G | A | T | , | T | G | A | –3′ | | – | C | T | A | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTACTT-3′ and 5′-TCAATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 1a97_c405
| 5′– | A | T | G | , | C | A | G | – | | 3′– | T | A | C | , | G | T | C | – | | | | – | G | G | T | , | A | C | A | , | G | A | G | , | G | A | C | , | A | C | C | , | T | G | A | , | A | A | C | , | G | C | T | – | | – | C | C | A | , | T | G | T | , | C | T | C | , | C | T | G | , | T | G | G | , | A | C | T | , | T | T | G | , | C | G | A | – | | | | – | C | A | A | , | A | C | T | –3′ | | – | G | T | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGCAG-3′ and 5′-AGTTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC edbe_f494
| 5′– | A | C | C | , | T | T | G | – | | 3′– | T | G | G | , | A | A | C | – | | | | – | G | A | C | , | T | C | G | , | T | C | G | , | T | G | A | , | A | T | G | , | T | A | C | , | G | C | T | , | A | C | T | – | | – | C | T | G | , | A | G | C | , | A | G | C | , | A | C | T | , | T | A | C | , | A | T | G | , | C | G | A | , | T | G | A | – | | | | – | G | G | T | , | C | G | A | –3′ | | – | C | C | A | , | G | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACCTTG-3′ and 5′-TCGACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 17f0_b030
| 5′– | A | T | G | , | G | T | C | – | | 3′– | T | A | C | , | C | A | G | – | | | | – | A | C | T | , | T | T | G | , | G | T | A | , | C | T | T | , | A | C | G | , | G | T | G | , | T | G | C | , | A | G | C | – | | – | T | G | A | , | A | A | C | , | C | A | T | , | G | A | A | , | T | G | C | , | C | A | C | , | A | C | G | , | T | C | G | – | | | | – | T | T | C | , | A | T | G | –3′ | | – | A | A | G | , | T | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGGTC-3′ and 5′-CATGAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5fb5_df51
| 5′– | A | G | T | , | A | A | G | – | | 3′– | T | C | A | , | T | T | C | – | | | | – | T | G | A | , | T | C | C | , | A | T | G | , | T | A | C | , | G | A | T | , | T | C | T | , | T | G | G | , | A | T | C | – | | – | A | C | T | , | A | G | G | , | T | A | C | , | A | T | G | , | C | T | A | , | A | G | A | , | A | C | C | , | T | A | G | – | | | | – | A | A | C | , | T | G | C | –3′ | | – | T | T | G | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTAAG-3′ and 5′-GCAGTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6df8_fc64
| 5′– | C | G | T | , | T | C | A | – | | 3′– | G | C | A | , | A | G | T | – | | | | – | C | G | A | , | G | T | C | , | G | T | A | , | C | T | G | , | C | T | C | , | C | G | A | , | G | A | C | , | C | A | T | – | | – | G | C | T | , | C | A | G | , | C | A | T | , | G | A | C | , | G | A | G | , | G | C | T | , | C | T | G | , | G | T | A | – | | | | – | A | C | T | , | T | C | G | –3′ | | – | T | G | A | , | A | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTTCA-3′ and 5′-CGAAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC bd84_040c
| 5′– | C | A | T | , | C | G | A | – | | 3′– | G | T | A | , | G | C | T | – | | | | – | A | G | A | , | T | A | C | , | A | C | A | , | G | C | C | , | A | A | C | , | G | A | T | , | C | A | C | , | T | C | G | – | | – | T | C | T | , | A | T | G | , | T | G | T | , | C | G | G | , | T | T | G | , | C | T | A | , | G | T | G | , | A | G | C | – | | | | – | A | A | G | , | T | C | G | –3′ | | – | T | T | C | , | A | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATCGA-3′ and 5′-CGACTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 419c_c676
| 5′– | A | G | T | , | C | A | G | – | | 3′– | T | C | A | , | G | T | C | – | | | | – | A | T | T | , | G | T | C | , | C | T | T | , | A | G | C | , | A | G | T | , | G | G | G | , | T | A | A | , | G | G | C | – | | – | T | A | A | , | C | A | G | , | G | A | A | , | T | C | G | , | T | C | A | , | C | C | C | , | A | T | T | , | C | C | G | – | | | | – | C | A | T | , | C | A | A | –3′ | | – | G | T | A | , | G | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTCAG-3′ and 5′-TTGATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7163_40a5
| 5′– | G | T | C | , | T | C | A | – | | 3′– | C | A | G | , | A | G | T | – | | | | – | T | C | G | , | A | C | G | , | A | C | T | , | G | G | A | , | T | A | C | , | T | A | C | , | T | G | A | , | G | G | C | – | | – | A | G | C | , | T | G | C | , | T | G | A | , | C | C | T | , | A | T | G | , | A | T | G | , | A | C | T | , | C | C | G | – | | | | – | G | T | T | , | G | A | A | –3′ | | – | C | A | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCTCA-3′ and 5′-TTCAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC b067_52f6
| 5′– | G | C | T | , | G | T | A | – | | 3′– | C | G | A | , | C | A | T | – | | | | – | C | G | A | , | T | T | C | , | C | A | G | , | A | C | C | , | G | T | G | , | C | G | T | , | G | G | T | , | C | A | T | – | | – | G | C | T | , | A | A | G | , | G | T | C | , | T | G | G | , | C | A | C | , | G | C | A | , | C | C | A | , | G | T | A | – | | | | – | A | A | T | , | C | T | G | –3′ | | – | T | T | A | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTGTA-3′ and 5′-CAGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ec46_2fd8
| 5′– | T | C | G | , | A | T | G | – | | 3′– | A | G | C | , | T | A | C | – | | | | – | C | A | T | , | G | G | T | , | T | G | T | , | C | T | T | , | G | G | A | , | T | T | G | , | T | G | C | , | A | T | A | – | | – | G | T | A | , | C | C | A | , | A | C | A | , | G | A | A | , | C | C | T | , | A | A | C | , | A | C | G | , | T | A | T | – | | | | – | T | A | C | , | T | T | G | –3′ | | – | A | T | G | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCGATG-3′ and 5′-CAAGTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 18e5_791a
| 5′– | C | C | T | , | C | C | A | – | | 3′– | G | G | A | , | G | G | T | – | | | | – | G | A | G | , | A | T | C | , | G | A | A | , | T | C | T | , | A | G | A | , | T | G | T | , | C | T | C | , | G | G | A | – | | – | C | T | C | , | T | A | G | , | C | T | T | , | A | G | A | , | T | C | T | , | A | C | A | , | G | A | G | , | C | C | T | – | | | | – | A | G | C | , | T | G | G | –3′ | | – | T | C | G | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTCCA-3′ and 5′-CCAGCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC de6f_dca4
| 5′– | T | G | A | , | C | C | G | – | | 3′– | A | C | T | , | G | G | C | – | | | | – | T | G | T | , | A | C | G | , | T | A | C | , | A | T | G | , | G | A | A | , | G | A | G | , | A | C | G | , | G | T | C | – | | – | A | C | A | , | T | G | C | , | A | T | G | , | T | A | C | , | C | T | T | , | C | T | C | , | T | G | C | , | C | A | G | – | | | | – | C | T | T | , | C | A | A | –3′ | | – | G | A | A | , | G | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGACCG-3′ and 5′-TTGAAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 165a_7085
| 5′– | T | G | G | , | A | T | C | – | | 3′– | A | C | C | , | T | A | G | – | | | | – | A | T | C | , | C | G | T | , | G | C | A | , | G | C | A | , | T | T | C | , | C | C | A | , | T | A | G | , | C | A | C | – | | – | T | A | G | , | G | C | A | , | C | G | T | , | C | G | T | , | A | A | G | , | G | G | T | , | A | T | C | , | G | T | G | – | | | | – | C | G | A | , | G | T | T | –3′ | | – | G | C | T | , | C | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGATC-3′ and 5′-AACTCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c773_8bcf
| 5′– | G | C | T | , | G | A | A | – | | 3′– | C | G | A | , | C | T | T | – | | | | – | A | G | C | , | C | T | T | , | G | T | A | , | A | C | A | , | T | G | A | , | C | G | A | , | A | C | G | , | T | A | C | – | | – | T | C | G | , | G | A | A | , | C | A | T | , | T | G | T | , | A | C | T | , | G | C | T | , | T | G | C | , | A | T | G | – | | | | – | A | T | C | , | T | A | C | –3′ | | – | T | A | G | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTGAA-3′ and 5′-GTAGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC f72c_eec2
| 5′– | C | G | G | , | T | T | A | – | | 3′– | G | C | C | , | A | A | T | – | | | | – | A | T | A | , | C | G | C | , | T | T | G | , | C | C | A | , | T | G | G | , | C | A | A | , | C | C | T | , | G | A | C | – | | – | T | A | T | , | G | C | G | , | A | A | C | , | G | G | T | , | A | C | C | , | G | T | T | , | G | G | A | , | C | T | G | – | | | | – | T | T | G | , | A | G | C | –3′ | | – | A | A | C | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGGTTA-3′ and 5′-GCTCAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 157c_cf44
| 5′– | G | T | C | , | C | G | A | – | | 3′– | C | A | G | , | G | C | T | – | | | | – | G | A | C | , | C | A | T | , | T | C | T | , | T | G | G | , | A | C | A | , | G | G | C | , | T | C | C | , | A | G | T | – | | – | C | T | G | , | G | T | A | , | A | G | A | , | A | C | C | , | T | G | T | , | C | C | G | , | A | G | G | , | T | C | A | – | | | | – | G | C | C | , | T | G | A | –3′ | | – | C | G | G | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCCGA-3′ and 5′-TCAGGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c6bc_915f
| 5′– | G | T | A | , | G | T | T | – | | 3′– | C | A | T | , | C | A | A | – | | | | – | T | C | A | , | C | G | T | , | A | C | C | , | T | A | G | , | T | T | T | , | C | C | G | , | T | C | A | , | G | G | G | – | | – | A | G | T | , | G | C | A | , | T | G | G | , | A | T | C | , | A | A | A | , | G | G | C | , | A | G | T | , | C | C | C | – | | | | – | G | A | C | , | A | A | T | –3′ | | – | C | T | G | , | T | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTAGTT-3′ and 5′-ATTGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 7463_8c43
| 5′– | A | C | T | , | A | T | C | – | | 3′– | T | G | A | , | T | A | G | – | | | | – | G | A | C | , | T | C | T | , | T | G | G | , | T | C | T | , | G | G | G | , | T | A | G | , | A | A | G | , | T | C | A | – | | – | C | T | G | , | A | G | A | , | A | C | C | , | A | G | A | , | C | C | C | , | A | T | C | , | T | T | C | , | A | G | T | – | | | | – | A | T | G | , | T | T | G | –3′ | | – | T | A | C | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTATC-3′ and 5′-CAACAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC b98a_1e17
| 5′– | C | A | G | , | G | C | T | – | | 3′– | G | T | C | , | C | G | A | – | | | | – | G | T | G | , | T | A | C | , | T | C | C | , | G | A | C | , | A | A | G | , | T | T | T | , | C | C | A | , | T | G | A | – | | – | C | A | C | , | A | T | G | , | A | G | G | , | C | T | G | , | T | T | C | , | A | A | A | , | G | G | T | , | A | C | T | – | | | | – | C | A | G | , | T | A | A | –3′ | | – | G | T | C | , | A | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGGCT-3′ and 5′-TTACTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 722e_2886
| 5′– | G | T | T | , | T | G | A | – | | 3′– | C | A | A | , | A | C | T | – | | | | – | G | C | A | , | G | A | T | , | T | G | G | , | G | A | G | , | C | A | T | , | A | C | G | , | A | T | C | , | G | C | A | – | | – | C | G | T | , | C | T | A | , | A | C | C | , | C | T | C | , | G | T | A | , | T | G | C | , | T | A | G | , | C | G | T | – | | | | – | G | A | A | , | T | G | A | –3′ | | – | C | T | T | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTTGA-3′ and 5′-TCATTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3285_2ceb
| 5′– | G | T | C | , | T | C | A | – | | 3′– | C | A | G | , | A | G | T | – | | | | – | A | C | T | , | G | G | A | , | C | G | G | , | T | C | G | , | C | T | T | , | T | C | A | , | T | G | A | , | C | G | C | – | | – | T | G | A | , | C | C | T | , | G | C | C | , | A | G | C | , | G | A | A | , | A | G | T | , | A | C | T | , | G | C | G | – | | | | – | C | G | A | , | G | G | T | –3′ | | – | G | C | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCTCA-3′ and 5′-ACCTCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2b26_542f
| 5′– | C | T | G | , | G | A | A | – | | 3′– | G | A | C | , | C | T | T | – | | | | – | T | G | A | , | C | C | C | , | T | C | A | , | T | A | C | , | G | A | T | , | G | G | T | , | A | G | A | , | T | G | C | – | | – | A | C | T | , | G | G | G | , | A | G | T | , | A | T | G | , | C | T | A | , | C | C | A | , | T | C | T | , | A | C | G | – | | | | – | C | G | A | , | A | C | T | –3′ | | – | G | C | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGGAA-3′ and 5′-AGTTCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 8037_01ec
| 5′– | A | G | T | , | A | G | G | – | | 3′– | T | C | A | , | T | C | C | – | | | | – | C | G | T | , | T | G | A | , | G | T | G | , | C | A | T | , | C | A | A | , | C | G | A | , | G | G | A | , | T | C | T | – | | – | G | C | A | , | A | C | T | , | C | A | C | , | G | T | A | , | G | T | T | , | G | C | T | , | C | C | T | , | A | G | A | – | | | | – | T | G | A | , | T | G | G | –3′ | | – | A | C | T | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTAGG-3′ and 5′-CCATCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3388_4ed4
| 5′– | C | C | T | , | C | A | A | – | | 3′– | G | G | A | , | G | T | T | – | | | | – | C | G | A | , | T | C | A | , | A | T | G | , | G | A | A | , | A | G | C | , | G | A | G | , | G | T | A | , | T | C | T | – | | – | G | C | T | , | A | G | T | , | T | A | C | , | C | T | T | , | T | C | G | , | C | T | C | , | C | A | T | , | A | G | A | – | | | | – | C | G | T | , | T | C | A | –3′ | | – | G | C | A | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTCAA-3′ and 5′-TGAACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 952d_649c
| 5′– | G | T | C | , | C | C | A | – | | 3′– | C | A | G | , | G | G | T | – | | | | – | C | A | G | , | C | G | T | , | T | T | C | , | C | C | A | , | A | C | T | , | A | T | C | , | G | G | T | , | C | T | A | – | | – | G | T | C | , | G | C | A | , | A | A | G | , | G | G | T | , | T | G | A | , | T | A | G | , | C | C | A | , | G | A | T | – | | | | – | G | T | A | , | G | A | A | –3′ | | – | C | A | T | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCCCA-3′ and 5′-TTCTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b90c_5a56
| 5′– | C | C | T | , | A | C | T | – | | 3′– | G | G | A | , | T | G | A | – | | | | – | A | C | T | , | T | G | G | , | A | C | C | , | A | A | T | , | C | A | C | , | A | G | A | , | T | A | G | , | G | A | C | – | | – | T | G | A | , | A | C | C | , | T | G | G | , | T | T | A | , | G | T | G | , | T | C | T | , | A | T | C | , | C | T | G | – | | | | – | G | A | C | , | A | C | T | –3′ | | – | C | T | G | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTACT-3′ and 5′-AGTGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC eb70_546b
| 5′– | G | T | A | , | A | G | T | – | | 3′– | C | A | T | , | T | C | A | – | | | | – | G | A | C | , | T | C | T | , | T | A | G | , | T | G | A | , | G | G | G | , | T | A | C | , | C | G | A | , | T | C | T | – | | – | C | T | G | , | A | G | A | , | A | T | C | , | A | C | T | , | C | C | C | , | A | T | G | , | G | C | T | , | A | G | A | – | | | | – | C | T | A | , | G | T | A | –3′ | | – | G | A | T | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTAAGT-3′ and 5′-TACTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9a71_27e4
| 5′– | T | T | G | , | C | A | G | – | | 3′– | A | A | C | , | G | T | C | – | | | | – | G | T | G | , | A | C | T | , | G | C | C | , | T | G | C | , | T | C | G | , | T | G | C | , | A | T | G | , | A | C | A | – | | – | C | A | C | , | T | G | A | , | C | G | G | , | A | C | G | , | A | G | C | , | A | C | G | , | T | A | C | , | T | G | T | – | | | | – | T | G | A | , | T | G | C | –3′ | | – | A | C | T | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTGCAG-3′ and 5′-GCATCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 837b_7937
| 5′– | C | A | G | , | G | T | T | – | | 3′– | G | T | C | , | C | A | A | – | | | | – | G | T | A | , | A | G | C | , | T | A | C | , | T | T | C | , | A | C | G | , | C | T | A | , | C | A | G | , | T | A | A | – | | – | C | A | T | , | T | C | G | , | A | T | G | , | A | A | G | , | T | G | C | , | G | A | T | , | G | T | C | , | A | T | T | – | | | | – | A | A | T | , | C | G | G | –3′ | | – | T | T | A | , | G | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGGTT-3′ and 5′-CCGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 8fb4_90c1
| 5′– | T | T | G | , | A | A | G | – | | 3′– | A | A | C | , | T | T | C | – | | | | – | T | C | G | , | A | C | C | , | G | A | T | , | G | T | T | , | G | A | T | , | A | G | G | , | C | A | T | , | T | C | G | – | | – | A | G | C | , | T | G | G | , | C | T | A | , | C | A | A | , | C | T | A | , | T | C | C | , | G | T | A | , | A | G | C | – | | | | – | A | A | T | , | C | C | G | –3′ | | – | T | T | A | , | G | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTGAAG-3′ and 5′-CGGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 81b3_2a56
| 5′– | G | G | T | , | G | A | T | – | | 3′– | C | C | A | , | C | T | A | – | | | | – | T | G | T | , | C | A | A | , | G | C | G | , | T | T | A | , | C | C | A | , | C | T | G | , | T | C | C | , | A | C | G | – | | – | A | C | A | , | G | T | T | , | C | G | C | , | A | A | T | , | G | G | T | , | G | A | C | , | A | G | G | , | T | G | C | – | | | | – | G | A | C | , | A | T | T | –3′ | | – | C | T | G | , | T | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGTGAT-3′ and 5′-AATGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC cf43_b972
| 5′– | T | G | A | , | T | C | C | – | | 3′– | A | C | T | , | A | G | G | – | | | | – | A | G | C | , | T | C | G | , | A | C | G | , | A | C | T | , | C | A | A | , | C | A | A | , | C | C | G | , | A | T | C | – | | – | T | C | G | , | A | G | C | , | T | G | C | , | T | G | A | , | G | T | T | , | G | T | T | , | G | G | C | , | T | A | G | – | | | | – | G | A | T | , | G | T | T | –3′ | | – | C | T | A | , | C | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGATCC-3′ and 5′-AACATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC d457_99fd
| 5′– | T | G | A | , | G | A | C | – | | 3′– | A | C | T | , | C | T | G | – | | | | – | T | A | C | , | T | G | T | , | A | A | G | , | T | G | G | , | T | C | T | , | T | C | G | , | C | T | C | , | A | C | G | – | | – | A | T | G | , | A | C | A | , | T | T | C | , | A | C | C | , | A | G | A | , | A | G | C | , | G | A | G | , | T | G | C | – | | | | – | C | A | A | , | C | T | A | –3′ | | – | G | T | T | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAGAC-3′ and 5′-TAGTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 7c63_4d64
| 5′– | A | A | G | , | C | T | G | – | | 3′– | T | T | C | , | G | A | C | – | | | | – | G | T | A | , | G | T | C | , | G | T | T | , | C | C | A | , | G | G | A | , | C | A | T | , | T | G | A | , | C | C | A | – | | – | C | A | T | , | C | A | G | , | C | A | A | , | G | G | T | , | C | C | T | , | G | T | A | , | A | C | T | , | G | G | T | – | | | | – | C | G | A | , | T | C | A | –3′ | | – | G | C | T | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AAGCTG-3′ and 5′-TGATCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC e56f_53e8
| 5′– | C | A | T | , | G | G | A | – | | 3′– | G | T | A | , | C | C | T | – | | | | – | G | G | A | , | T | C | T | , | G | G | G | , | T | A | A | , | C | G | A | , | G | C | A | , | A | G | G | , | T | C | A | – | | – | C | C | T | , | A | G | A | , | C | C | C | , | A | T | T | , | G | C | T | , | C | G | T | , | T | C | C | , | A | G | T | – | | | | – | G | G | T | , | T | G | A | –3′ | | – | C | C | A | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATGGA-3′ and 5′-TCAACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 6ccd_8f1d
| 5′– | C | G | A | , | T | G | A | – | | 3′– | G | C | T | , | A | C | T | – | | | | – | T | A | T | , | G | C | T | , | A | C | C | , | T | T | C | , | T | T | C | , | G | C | A | , | T | C | C | , | G | A | G | – | | – | A | T | A | , | C | G | A | , | T | G | G | , | A | A | G | , | A | A | G | , | C | G | T | , | A | G | G | , | C | T | C | – | | | | – | A | G | G | , | G | T | C | –3′ | | – | T | C | C | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGATGA-3′ and 5′-GACCCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC a1e1_810a
| 5′– | T | T | A | , | C | C | G | – | | 3′– | A | A | T | , | G | G | C | – | | | | – | A | T | C | , | A | T | G | , | A | G | C | , | T | T | C | , | A | T | G | , | G | A | A | , | G | T | A | , | T | C | C | – | | – | T | A | G | , | T | A | C | , | T | C | G | , | A | A | G | , | T | A | C | , | C | T | T | , | C | A | T | , | A | G | G | – | | | | – | T | C | A | , | T | G | C | –3′ | | – | A | G | T | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTACCG-3′ and 5′-GCATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC bdb4_e442
| 5′– | A | C | T | , | A | G | C | – | | 3′– | T | G | A | , | T | C | G | – | | | | – | G | T | T | , | T | C | A | , | C | C | G | , | T | G | C | , | C | T | G | , | G | T | C | , | T | G | T | , | C | G | A | – | | – | C | A | A | , | A | G | T | , | G | G | C | , | A | C | G | , | G | A | C | , | C | A | G | , | A | C | A | , | G | C | T | – | | | | – | C | C | T | , | C | C | A | –3′ | | – | G | G | A | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTAGC-3′ and 5′-TGGAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 17b3_e64b
| 5′– | C | A | T | , | A | G | T | – | | 3′– | G | T | A | , | T | C | A | – | | | | – | C | T | A | , | G | G | C | , | A | T | T | , | G | C | C | , | A | A | A | , | G | A | G | , | T | C | C | , | A | G | A | – | | – | G | A | T | , | C | C | G | , | T | A | A | , | C | G | G | , | T | T | T | , | C | T | C | , | A | G | G | , | T | C | T | – | | | | – | A | A | T | , | G | T | C | –3′ | | – | T | T | A | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATAGT-3′ and 5′-GACATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 22bf_d09b
| 5′– | C | T | A | , | C | C | T | – | | 3′– | G | A | T | , | G | G | A | – | | | | – | A | C | T | , | A | T | G | , | T | A | G | , | A | G | T | , | G | A | A | , | C | G | A | , | T | G | C | , | A | A | G | – | | – | T | G | A | , | T | A | C | , | A | T | C | , | T | C | A | , | C | T | T | , | G | C | T | , | A | C | G | , | T | T | C | – | | | | – | C | C | A | , | C | A | T | –3′ | | – | G | G | T | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTACCT-3′ and 5′-ATGTGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0e18_2446
| 5′– | A | T | G | , | A | C | G | – | | 3′– | T | A | C | , | T | G | C | – | | | | – | T | T | T | , | G | A | C | , | C | G | T | , | G | A | C | , | G | A | T | , | T | G | T | , | T | C | A | , | T | T | G | – | | – | A | A | A | , | C | T | G | , | G | C | A | , | C | T | G | , | C | T | A | , | A | C | A | , | A | G | T | , | A | A | C | – | | | | – | A | G | C | , | T | T | G | –3′ | | – | T | C | G | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGACG-3′ and 5′-CAAGCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2110_c42f
| 5′– | A | G | T | , | C | A | G | – | | 3′– | T | C | A | , | G | T | C | – | | | | – | T | G | T | , | G | C | A | , | C | T | G | , | T | C | G | , | T | C | T | , | T | C | T | , | A | G | G | , | T | T | C | – | | – | A | C | A | , | C | G | T | , | G | A | C | , | A | G | C | , | A | G | A | , | A | G | A | , | T | C | C | , | A | A | G | – | | | | – | C | A | T | , | G | C | T | –3′ | | – | G | T | A | , | C | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTCAG-3′ and 5′-AGCATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 347d_e440
| 5′– | T | G | C | , | G | A | C | – | | 3′– | A | C | G | , | C | T | G | – | | | | – | C | C | T | , | A | T | G | , | T | C | C | , | T | C | G | , | A | A | G | , | G | G | T | , | G | C | A | , | T | G | A | – | | – | G | G | A | , | T | A | C | , | A | G | G | , | A | G | C | , | T | T | C | , | C | C | A | , | C | G | T | , | A | C | T | – | | | | – | T | G | G | , | A | T | G | –3′ | | – | A | C | C | , | T | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCGAC-3′ and 5′-CATCCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 61c3_77fe
| 5′– | C | A | G | , | G | C | T | – | | 3′– | G | T | C | , | C | G | A | – | | | | – | T | C | G | , | G | T | A | , | A | G | C | , | G | A | G | , | G | T | G | , | A | G | C | , | T | C | G | , | C | A | C | – | | – | A | G | C | , | C | A | T | , | T | C | G | , | C | T | C | , | C | A | C | , | T | C | G | , | A | G | C | , | G | T | G | – | | | | – | T | G | A | , | T | G | G | –3′ | | – | A | C | T | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGGCT-3′ and 5′-CCATCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 74e3_0af7
| 5′– | C | T | G | , | A | A | T | – | | 3′– | G | A | C | , | T | T | A | – | | | | – | T | A | A | , | C | G | A | , | G | C | C | , | A | A | G | , | C | A | C | , | A | A | C | , | T | A | G | , | G | T | C | – | | – | A | T | T | , | G | C | T | , | C | G | G | , | T | T | C | , | G | T | G | , | T | T | G | , | A | T | C | , | C | A | G | – | | | | – | A | G | C | , | C | T | G | –3′ | | – | T | C | G | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGAAT-3′ and 5′-CAGGCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC bfb6_907d
| 5′– | C | A | T | , | C | C | A | – | | 3′– | G | T | A | , | G | G | T | – | | | | – | A | G | C | , | T | T | G | , | T | C | G | , | G | A | C | , | T | C | T | , | C | T | G | , | A | G | T | , | G | A | C | – | | – | T | C | G | , | A | A | C | , | A | G | C | , | C | T | G | , | A | G | A | , | G | A | C | , | T | C | A | , | C | T | G | – | | | | – | A | C | T | , | T | G | C | –3′ | | – | T | G | A | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATCCA-3′ and 5′-GCAAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC eed9_662c
| 5′– | G | C | A | , | C | T | A | – | | 3′– | C | G | T | , | G | A | T | – | | | | – | G | C | T | , | C | G | A | , | T | T | G | , | G | G | T | , | C | C | G | , | T | G | C | , | A | G | C | , | G | A | T | – | | – | C | G | A | , | G | C | T | , | A | A | C | , | C | C | A | , | G | G | C | , | A | C | G | , | T | C | G | , | C | T | A | – | | | | – | A | G | G | , | T | T | C | –3′ | | – | T | C | C | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCACTA-3′ and 5′-GAACCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ea03_2bdc
| 5′– | C | A | A | , | C | G | T | – | | 3′– | G | T | T | , | G | C | A | – | | | | – | T | G | C | , | G | A | C | , | A | T | G | , | C | C | T | , | C | C | A | , | G | G | G | , | A | G | T | , | T | G | C | – | | – | A | C | G | , | C | T | G | , | T | A | C | , | G | G | A | , | G | G | T | , | C | C | C | , | T | C | A | , | A | C | G | – | | | | – | T | C | C | , | A | G | C | –3′ | | – | A | G | G | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAACGT-3′ and 5′-GCTGGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 8166_0feb
| 5′– | C | A | G | , | G | G | T | – | | 3′– | G | T | C | , | C | C | A | – | | | | – | T | C | G | , | A | C | T | , | T | G | C | , | C | T | G | , | C | A | T | , | G | T | A | , | C | G | C | , | A | T | C | – | | – | A | G | C | , | T | G | A | , | A | C | G | , | G | A | C | , | G | T | A | , | C | A | T | , | G | C | G | , | T | A | G | – | | | | – | C | T | G | , | T | T | A | –3′ | | – | G | A | C | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGGGT-3′ and 5′-TAACAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 59ef_2a3d
| 5′– | A | G | T | , | C | C | G | – | | 3′– | T | C | A | , | G | G | C | – | | | | – | A | A | C | , | T | G | A | , | G | G | A | , | G | G | T | , | C | A | C | , | G | A | C | , | C | G | A | , | A | T | G | – | | – | T | T | G | , | A | C | T | , | C | C | T | , | C | C | A | , | G | T | G | , | C | T | G | , | G | C | T | , | T | A | C | – | | | | – | G | G | T | , | T | G | A | –3′ | | – | C | C | A | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTCCG-3′ and 5′-TCAACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC ba50_1417
| 5′– | T | A | G | , | G | A | C | – | | 3′– | A | T | C | , | C | T | G | – | | | | – | C | G | T | , | C | T | A | , | T | C | G | , | A | G | G | , | A | G | C | , | G | A | C | , | C | T | A | , | G | G | A | – | | – | G | C | A | , | G | A | T | , | A | G | C | , | T | C | C | , | T | C | G | , | C | T | G | , | G | A | T | , | C | C | T | – | | | | – | T | T | C | , | A | G | G | –3′ | | – | A | A | G | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGGAC-3′ and 5′-CCTGAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 465f_8edd
| 5′– | A | G | C | , | T | A | C | – | | 3′– | T | C | G | , | A | T | G | – | | | | – | T | C | T | , | T | A | G | , | A | A | G | , | C | T | T | , | G | G | A | , | T | T | G | , | A | G | A | , | C | T | C | – | | – | A | G | A | , | A | T | C | , | T | T | C | , | G | A | A | , | C | C | T | , | A | A | C | , | T | C | T | , | G | A | G | – | | | | – | T | C | C | , | C | A | G | –3′ | | – | A | G | G | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGCTAC-3′ and 5′-CTGGGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 730e_9750
| 5′– | G | A | A | , | A | C | T | – | | 3′– | C | T | T | , | T | G | A | – | | | | – | A | G | T | , | C | G | T | , | T | C | G | , | T | A | C | , | C | T | G | , | T | C | A | , | C | T | C | , | A | G | C | – | | – | T | C | A | , | G | C | A | , | A | G | C | , | A | T | G | , | G | A | C | , | A | G | T | , | G | A | G | , | T | C | G | – | | | | – | G | C | T | , | T | C | A | –3′ | | – | C | G | A | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GAAACT-3′ and 5′-TGAAGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect