MC 021a_bdf7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | C | , | G | A | A | , | G | C | C | , | T | A | C | , | G | G | A | – | | – | C | A | G | , | C | T | T | , | C | G | G | , | A | T | G | , | C | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0dea_0ae4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | T | , | G | T | G | , | T | A | T | , | C | G | T | , | C | A | G | – | | – | T | G | A | , | C | A | C | , | A | T | A | , | G | C | A | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC a771_19c9
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | T | , | A | G | T | , | A | G | T | , | C | A | A | , | T | G | T | – | | – | C | G | A | , | T | C | A | , | T | C | A | , | G | T | T | , | A | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 952b_eea5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | A | G | C | , | G | A | C | , | T | G | C | , | A | G | T | – | | – | C | T | A | , | T | C | G | , | C | T | G | , | A | C | G | , | T | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 4729_2215
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | C | T | T | , | G | T | G | , | T | C | G | , | A | T | C | – | | – | T | C | A | , | G | A | A | , | C | A | C | , | A | G | C | , | T | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5569_55ea
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | A | , | T | G | G | , | G | T | C | , | A | C | A | , | G | T | A | – | | – | G | T | T | , | A | C | C | , | C | A | G | , | T | G | T | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 8764_f996
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | T | C | G | , | C | T | T | , | T | G | T | , | A | T | C | – | | – | T | A | G | , | A | G | C | , | G | A | A | , | A | C | A | , | T | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4edf_9b92
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | T | , | A | G | C | , | T | A | A | , | C | C | G | , | A | T | T | – | | – | G | A | A | , | T | C | G | , | A | T | T | , | G | G | C | , | T | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC fb21_369f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | T | , | C | A | C | , | T | C | G | , | A | A | C | , | C | T | G | – | | – | A | C | A | , | G | T | G | , | A | G | C | , | T | T | G | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC c27e_f2ac
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | A | T | A | , | C | A | G | , | G | T | T | , | C | A | T | – | | – | C | T | G | , | T | A | T | , | G | T | C | , | C | A | A | , | G | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 8b64_37d0
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | G | A | G | , | T | G | A | , | T | T | C | , | T | G | A | – | | – | G | A | T | , | C | T | C | , | A | C | T | , | A | A | G | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 1009_ac15
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | T | , | A | A | C | , | C | C | T | , | C | A | A | , | C | G | T | – | | – | G | C | A | , | T | T | G | , | G | G | A | , | G | T | T | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 38e2_42ca
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | A | , | G | C | T | , | A | T | C | , | T | G | A | , | G | C | G | – | | – | T | G | T | , | C | G | A | , | T | A | G | , | A | C | T | , | C | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC a2ac_6bb3
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | T | , | C | A | A | , | C | T | C | , | A | T | T | , | C | G | A | – | | – | G | C | A | , | G | T | T | , | G | A | G | , | T | A | A | , | G | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 0b66_3a14
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | G | A | C | , | A | T | T | , | G | G | G | , | A | C | T | – | | – | G | T | A | , | C | T | G | , | T | A | A | , | C | C | C | , | T | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC a354_0dce
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | G | , | T | A | T | , | G | T | G | , | A | G | C | , | G | T | T | – | | – | G | C | C | , | A | T | A | , | C | A | C | , | T | C | G | , | C | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 0198_d361
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | G | , | T | C | A | , | G | G | G | , | T | A | G | , | G | C | T | – | | – | C | G | C | , | A | G | T | , | C | C | C | , | A | T | C | , | C | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c484_9e8f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | T | T | C | , | C | A | C | , | G | G | T | , | A | T | C | – | | – | T | G | C | , | A | A | G | , | G | T | G | , | C | C | A | , | T | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 2e9a_8d67
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | G | , | T | C | T | , | C | C | C | , | A | T | G | , | T | C | G | – | | – | A | T | C | , | A | G | A | , | G | G | G | , | T | A | C | , | A | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3087_66ab
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | T | C | A | , | C | G | C | , | T | T | G | , | C | A | G | – | | – | T | A | C | , | A | G | T | , | G | C | G | , | A | A | C | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b436_56d8
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | A | G | C | , | C | T | A | , | G | C | T | , | A | C | A | – | | – | G | A | T | , | T | C | G | , | G | A | T | , | C | G | A | , | T | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 6f63_7604
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | T | , | C | G | T | , | C | G | G | , | T | A | T | , | C | G | G | – | | – | A | T | A | , | G | C | A | , | G | C | C | , | A | T | A | , | G | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 6604_8ed5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | T | , | G | A | G | , | T | C | A | , | G | T | T | , | C | A | G | – | | – | T | G | A | , | C | T | C | , | A | G | T | , | C | A | A | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 4ef1_b561
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | C | , | T | C | A | , | G | T | A | , | T | C | G | , | C | A | T | – | | – | G | C | G | , | A | G | T | , | C | A | T | , | A | G | C | , | G | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 46ff_fe30
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | A | , | A | C | G | , | A | G | G | , | A | A | A | , | C | T | G | – | | – | A | G | T | , | T | G | C | , | T | C | C | , | T | T | T | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC b88e_874d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | T | , | C | A | G | , | A | T | C | , | A | C | A | , | G | C | T | – | | – | G | C | A | , | G | T | C | , | T | A | G | , | T | G | T | , | C | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 3188_f565
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | T | G | A | , | C | T | G | , | C | G | T | , | C | A | G | – | | – | T | G | C | , | A | C | T | , | G | A | C | , | G | C | A | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8dc3_0f2c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | T | , | G | A | A | , | G | G | A | , | C | A | A | , | T | G | G | – | | – | T | G | A | , | C | T | T | , | C | C | T | , | G | T | T | , | A | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC d39e_fc53
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | G | , | T | C | C | , | A | T | T | , | G | T | T | , | G | A | C | – | | – | T | C | C | , | A | G | G | , | T | A | A | , | C | A | A | , | C | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 7576_1d40
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | C | , | T | A | G | , | T | C | G | , | T | G | C | , | C | T | A | – | | – | C | C | G | , | A | T | C | , | A | G | C | , | A | C | G | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 753e_544e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | C | , | A | C | T | , | T | G | C | , | T | C | A | , | G | T | T | – | | – | C | G | G | , | T | G | A | , | A | C | G | , | A | G | T | , | C | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 904b_5475
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | C | , | T | C | G | , | A | T | T | , | G | A | T | , | C | T | C | – | | – | T | G | G | , | A | G | C | , | T | A | A | , | C | T | A | , | G | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 019d_f343
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | C | , | A | G | T | , | T | T | C | , | A | T | T | , | G | G | C | – | | – | A | A | G | , | T | C | A | , | A | A | G | , | T | A | A | , | C | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 922f_c1be
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | A | , | G | C | T | , | C | C | A | , | C | A | G | , | T | A | C | – | | – | A | T | T | , | C | G | A | , | G | G | T | , | G | T | C | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC b1e8_88eb
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | A | , | T | G | A | , | C | A | A | , | G | C | A | , | G | T | T | – | | – | C | G | T | , | A | C | T | , | G | T | T | , | C | G | T | , | C | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 561f_91c1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | G | , | T | C | A | , | A | G | C | , | A | A | C | , | G | G | T | – | | – | C | T | C | , | A | G | T | , | T | C | G | , | T | T | G | , | C | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC d1d8_2d4c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | A | , | C | G | G | , | T | C | A | , | G | G | A | , | C | T | C | – | | – | A | A | T | , | G | C | C | , | A | G | T | , | C | C | T | , | G | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 335c_132b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | C | , | T | C | A | , | C | A | T | , | T | G | C | , | T | C | A | – | | – | C | G | G | , | A | G | T | , | G | T | A | , | A | C | G | , | A | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 5657_8d5d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | G | , | T | G | A | , | C | C | A | , | C | T | C | , | G | A | A | – | | – | G | C | C | , | A | C | T | , | G | G | T | , | G | A | G | , | C | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 7db8_519a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | T | , | G | A | A | , | C | A | G | , | C | G | T | , | C | A | C | – | | – | A | G | A | , | C | T | T | , | G | T | C | , | G | C | A | , | G | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6173_c483
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | C | , | A | T | G | , | A | C | G | , | A | T | C | , | C | G | T | – | | – | G | C | G | , | T | A | C | , | T | G | C | , | T | A | G | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 9594_406a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | T | , | G | T | C | , | G | A | G | , | A | T | C | , | C | A | G | – | | – | A | T | A | , | C | A | G | , | C | T | C | , | T | A | G | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC e26b_d8b2
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | G | G | C | , | T | T | C | , | C | T | A | , | C | G | A | – | | – | G | T | A | , | C | C | G | , | A | A | G | , | G | A | T | , | G | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 87ca_67d1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | A | , | A | T | G | , | G | G | A | , | G | C | T | , | A | C | A | – | | – | G | G | T | , | T | A | C | , | C | C | T | , | C | G | A | , | T | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 629f_464d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | T | , | G | C | G | , | A | G | C | , | T | G | T | , | T | A | C | – | | – | T | T | A | , | C | G | C | , | T | C | G | , | A | C | A | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC e099_65dc
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | A | , | C | G | T | , | T | T | C | , | A | C | C | , | A | G | T | – | | – | G | T | T | , | G | C | A | , | A | A | G | , | T | G | G | , | T | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC e70e_454d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | T | , | A | G | A | , | C | T | A | , | T | G | C | , | G | A | A | – | | – | G | G | A | , | T | C | T | , | G | A | T | , | A | C | G | , | C | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 6e10_b278
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | C | , | T | G | A | , | G | A | A | , | C | G | A | , | C | C | T | – | | – | G | A | G | , | A | C | T | , | C | T | T | , | G | C | T | , | G | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC f72a_d168
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | G | A | G | , | C | C | A | , | G | C | G | , | A | A | T | – | | – | G | A | T | , | C | T | C | , | G | G | T | , | C | G | C | , | T | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 86a9_efc5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | A | T | T | , | G | T | G | , | C | A | A | , | T | G | A | – | | – | G | A | C | , | T | A | A | , | C | A | C | , | G | T | T | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC e842_cdc4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | G | , | C | T | A | , | C | T | G | , | A | A | C | , | G | T | C | – | | – | A | C | C | , | G | A | T | , | G | A | C | , | T | T | G | , | C | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 03c3_47a4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | G | , | A | C | G | , | T | A | A | , | G | A | T | , | C | G | T | – | | – | C | A | C | , | T | G | C | , | A | T | T | , | C | T | A | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC cdc7_e196
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | G | , | T | A | A | , | C | T | A | , | A | G | C | , | G | T | A | – | | – | G | C | C | , | A | T | T | , | G | A | T | , | T | C | G | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 5a41_7925
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | A | , | C | T | C | , | A | G | C | , | A | C | A | , | C | G | T | – | | – | C | A | T | , | G | A | G | , | T | C | G | , | T | G | T | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2632_bf4b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | A | , | T | C | G | , | C | T | T | , | T | C | A | , | G | T | T | – | | – | C | A | T | , | A | G | C | , | G | A | A | , | A | G | T | , | C | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC cad5_06fb
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | G | , | C | G | T | , | T | C | A | , | G | C | C | , | T | A | T | – | | – | G | T | C | , | G | C | A | , | A | G | T | , | C | G | G | , | A | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 0bf2_828c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | T | , | G | A | C | , | T | C | C | , | T | G | C | , | T | T | A | – | | – | C | G | A | , | C | T | G | , | A | G | G | , | A | C | G | , | A | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 38c8_1c98
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | A | , | T | C | G | , | G | T | C | , | G | T | G | , | A | C | T | – | | – | C | A | T | , | A | G | C | , | C | A | G | , | C | A | C | , | T | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0428_5ae7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | G | , | T | A | C | , | A | G | C | , | T | A | G | , | G | A | C | – | | – | T | T | C | , | A | T | G | , | T | C | G | , | A | T | C | , | C | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 6ca3_e3c5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | A | , | C | T | A | , | C | T | C | , | A | G | C | , | T | G | C | – | | – | T | C | T | , | G | A | T | , | G | A | G | , | T | C | G | , | A | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f886_66ab
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | A | , | G | A | C | , | C | C | A | , | T | A | C | , | A | C | G | – | | – | A | A | T | , | C | T | G | , | G | G | T | , | A | T | G | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 292e_ebeb
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | A | , | T | C | G | , | T | C | C | , | T | A | C | , | T | G | A | – | | – | G | T | T | , | A | G | C | , | A | G | G | , | A | T | G | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC a981_c44e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | C | T | T | , | T | G | T | , | A | T | C | , | T | C | G | – | | – | T | C | G | , | G | A | A | , | A | C | A | , | T | A | G | , | A | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC e6b1_5f1f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | T | A | T | , | C | G | C | , | T | A | T | , | C | G | C | – | | – | T | G | C | , | A | T | A | , | G | C | G | , | A | T | A | , | G | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 74b2_15d5
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | G | , | A | G | T | , | G | A | A | , | C | C | A | , | G | T | T | – | | – | C | G | C | , | T | C | A | , | C | T | T | , | G | G | T | , | C | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 9a64_429b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | G | , | A | C | T | , | C | C | A | , | G | A | T | , | G | C | C | – | | – | T | T | C | , | T | G | A | , | G | G | T | , | C | T | A | , | C | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4425_7c09
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | A | T | G | , | G | C | A | , | A | A | G | , | C | G | T | – | | – | C | T | G | , | T | A | C | , | C | G | T | , | T | T | C | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC f9ed_55fd
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | C | , | A | C | G | , | G | T | T | , | G | A | C | , | G | C | T | – | | – | C | A | G | , | T | G | C | , | C | A | A | , | C | T | G | , | C | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC ee88_bb3c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | A | , | T | T | G | , | T | T | C | , | C | A | A | , | G | A | T | – | | – | C | G | T | , | A | A | C | , | A | A | G | , | G | T | T | , | C | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 3f5a_c50f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | A | T | T | , | C | A | A | , | C | T | G | , | C | A | T | – | | – | C | T | G | , | T | A | A | , | G | T | T | , | G | A | C | , | G | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 153a_c21d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | G | , | A | C | T | , | C | T | G | , | A | T | G | , | A | C | A | – | | – | C | G | C | , | T | G | A | , | G | A | C | , | T | A | C | , | T | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4be3_0259
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | A | , | A | T | G | , | T | A | G | , | A | T | T | , | G | T | C | – | | – | T | G | T | , | T | A | C | , | A | T | C | , | T | A | A | , | C | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC f0e5_ea2d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | G | G | C | , | T | T | T | , | C | C | T | , | G | T | A | – | | – | C | T | A | , | C | C | G | , | A | A | A | , | G | G | A | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 142f_b4dc
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | G | G | A | , | G | G | A | , | C | T | A | , | G | A | T | – | | – | G | A | T | , | C | C | T | , | C | C | T | , | G | A | T | , | C | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC de81_8e1c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | C | , | A | G | T | , | C | G | A | , | C | T | A | , | C | T | G | – | | – | A | T | G | , | T | C | A | , | G | C | T | , | G | A | T | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 3246_82ab
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | C | , | G | A | T | , | A | G | G | , | C | T | T | , | A | G | G | – | | – | T | G | G | , | C | T | A | , | T | C | C | , | G | A | A | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 9a98_de85
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | C | , | T | A | G | , | C | G | A | , | T | C | A | , | G | A | T | – | | – | C | G | G | , | A | T | C | , | G | C | T | , | A | G | T | , | C | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC a070_c3a3
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | G | , | T | G | A | , | C | A | A | , | C | T | C | , | G | T | A | – | | – | G | C | C | , | A | C | T | , | G | T | T | , | G | A | G | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1532_cca1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | A | , | G | C | T | , | T | C | T | , | A | C | G | , | T | T | T | – | | – | G | C | T | , | C | G | A | , | A | G | A | , | T | G | C | , | A | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 785b_c0f1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | G | , | A | A | T | , | G | C | T | , | C | G | A | , | C | T | T | – | | – | G | G | C | , | T | T | A | , | C | G | A | , | G | C | T | , | G | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC b2ab_9249
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | T | , | G | G | A | , | A | G | C | , | G | A | C | , | A | T | T | – | | – | G | A | A | , | C | C | T | , | T | C | G | , | C | T | G | , | T | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC be85_4127
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | A | , | G | C | A | , | G | A | G | , | A | G | T | , | G | G | C | – | | – | A | G | T | , | C | G | T | , | C | T | C | , | T | C | A | , | C | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 541e_9608
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | A | , | C | T | T | , | C | T | C | , | A | T | C | , | T | G | A | – | | – | C | C | T | , | G | A | A | , | G | A | G | , | T | A | G | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c4ed_3466
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | T | , | C | A | A | , | G | T | C | , | A | C | G | , | A | G | T | – | | – | C | A | A | , | G | T | T | , | C | A | G | , | T | G | C | , | T | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 11e1_b2e4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | A | T | C | , | T | C | A | , | C | G | T | , | C | T | A | – | | – | G | A | C | , | T | A | G | , | A | G | T | , | G | C | A | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC a31e_b809
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | G | , | T | C | A | , | G | T | T | , | C | G | C | , | A | T | G | – | | – | A | C | C | , | A | G | T | , | C | A | A | , | G | C | G | , | T | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 87dc_b30a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | T | , | A | A | G | , | A | T | C | , | A | G | C | , | T | G | T | – | | – | C | G | A | , | T | T | C | , | T | A | G | , | T | C | G | , | A | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c39d_990b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | C | G | T | , | C | T | C | , | T | C | C | , | G | A | G | – | | – | T | C | A | , | G | C | A | , | G | A | G | , | A | G | G | , | C | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 3008_bdc7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | C | , | G | G | T | , | C | G | A | , | G | C | A | , | G | T | G | – | | – | T | T | G | , | C | C | A | , | G | C | T | , | C | G | T | , | C | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 92f4_9a06
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | A | , | G | A | C | , | A | A | C | , | C | T | A | , | A | G | G | – | | – | A | A | T | , | C | T | G | , | T | T | G | , | G | A | T | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5ee3_edd0
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | G | , | T | G | A | , | T | T | C | , | C | A | G | , | G | C | T | – | | – | G | C | C | , | A | C | T | , | A | A | G | , | G | T | C | , | C | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 270b_5cca
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | C | A | C | , | G | G | T | , | C | G | A | , | T | T | G | – | | – | T | A | C | , | G | T | G | , | C | C | A | , | G | C | T | , | A | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 06d4_0dd7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | G | T | G | , | T | G | T | , | G | C | A | , | C | T | G | – | | – | T | A | G | , | C | A | C | , | A | C | A | , | C | G | T | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC ca7a_3f9d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | A | , | C | A | T | , | C | T | T | , | G | G | C | , | T | G | A | – | | – | C | C | T | , | G | T | A | , | G | A | A | , | C | C | G | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3b5b_acdc
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | T | , | T | A | G | , | T | C | A | , | A | T | G | , | T | T | C | – | | – | A | G | A | , | A | T | C | , | A | G | T | , | T | A | C | , | A | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 6d49_0de2
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | A | , | G | T | A | , | C | A | T | , | G | G | C | , | A | A | T | – | | – | C | G | T | , | C | A | T | , | G | T | A | , | C | C | G | , | T | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 76cf_a764
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | A | C | T | , | C | C | A | , | G | C | T | , | G | T | A | – | | – | G | A | C | , | T | G | A | , | G | G | T | , | C | G | A | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f7c2_96e3
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | T | , | C | A | G | , | T | T | G | , | T | A | A | , | G | C | A | – | | – | G | G | A | , | G | T | C | , | A | A | C | , | A | T | T | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 4a27_2b1a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | C | G | A | , | C | C | G | , | A | T | C | , | C | G | T | – | | – | G | T | A | , | G | C | T | , | G | G | C | , | T | A | G | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC f2e1_6c60
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | C | , | G | G | T | , | T | G | A | , | G | T | G | , | A | T | C | – | | – | A | T | G | , | C | C | A | , | A | C | T | , | C | A | C | , | T | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 9c49_52eb
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | G | T | T | , | C | C | A | , | A | T | C | , | A | G | G | – | | – | T | C | G | , | C | A | A | , | G | G | T | , | T | A | G | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7540_07ef
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | T | A | C | , | A | C | T | , | A | C | A | , | T | G | C | – | | – | T | C | A | , | A | T | G | , | T | G | A | , | T | G | T | , | A | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5008_4a99
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | A | , | C | T | G | , | A | G | T | , | T | C | G | , | A | T | T | – | | – | C | C | T | , | G | A | C | , | T | C | A | , | A | G | C | , | T | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3d4d_ef4f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | C | , | A | G | T | , | T | T | C | , | G | C | A | , | C | T | G | – | | – | A | C | G | , | T | C | A | , | A | A | G | , | C | G | T | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 1b72_aa60
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | A | , | C | G | T | , | A | G | G | , | T | A | G | , | C | C | A | – | | – | G | C | T | , | G | C | A | , | T | C | C | , | A | T | C | , | G | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 01d4_d7c2
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | A | A | G | , | T | T | C | , | C | C | T | , | C | A | G | – | | – | T | A | G | , | T | T | C | , | A | A | G | , | G | G | A | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 7f00_7c6b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | A | , | T | C | C | , | T | T | T | , | C | A | G | , | C | A | T | – | | – | C | T | T | , | A | G | G | , | A | A | A | , | G | T | C | , | G | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 01b1_9098
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | T | G | A | , | A | A | G | , | C | T | C | , | A | G | G | – | | – | T | C | G | , | A | C | T | , | T | T | C | , | G | A | G | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 46b9_deaf
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | C | T | T | , | G | C | T | , | T | G | T | , | T | A | C | – | | – | T | C | G | , | G | A | A | , | C | G | A | , | A | C | A | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 1e51_835b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | G | , | A | C | C | , | C | A | G | , | A | A | G | , | T | A | C | – | | – | A | A | C | , | T | G | G | , | G | T | C | , | T | T | C | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC fb6c_f62b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | T | , | G | A | C | , | T | A | A | , | C | T | T | , | A | G | G | – | | – | T | A | A | , | C | T | G | , | A | T | T | , | G | A | A | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 64e2_ac8f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | A | , | C | G | T | , | G | T | C | , | C | A | G | , | T | C | C | – | | – | T | G | T | , | G | C | A | , | C | A | G | , | G | T | C | , | A | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 8147_333a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | C | , | A | G | G | , | A | A | T | , | G | G | T | , | T | A | C | – | | – | A | C | G | , | T | C | C | , | T | T | A | , | C | C | A | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 2eab_b8b4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | A | , | G | T | C | , | T | C | C | , | C | A | T | , | G | G | T | – | | – | C | A | T | , | C | A | G | , | A | G | G | , | G | T | A | , | C | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC eb73_8c42
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | C | A | T | , | C | C | T | , | C | A | G | , | T | T | G | – | | – | T | C | G | , | G | T | A | , | G | G | A | , | G | T | C | , | A | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC a3f6_8b1a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | G | , | T | A | C | , | A | C | A | , | C | T | A | , | G | C | A | – | | – | C | T | C | , | A | T | G | , | T | G | T | , | G | A | T | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9444_d98e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | T | A | T | , | C | C | T | , | C | C | T | , | A | A | G | – | | – | T | G | C | , | A | T | A | , | G | G | A | , | G | G | A | , | T | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1087_8927
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | C | T | T | , | C | A | G | , | C | T | C | , | G | C | A | – | | – | C | T | G | , | G | A | A | , | G | T | C | , | G | A | G | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC d1ea_39e1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | T | C | A | , | A | G | T | , | G | C | T | , | C | T | A | – | | – | G | A | C | , | A | G | T | , | T | C | A | , | C | G | A | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 6530_411d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | G | T | A | , | G | T | G | , | A | T | C | , | C | G | T | – | | – | G | A | T | , | C | A | T | , | C | A | C | , | T | A | G | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 1012_d4d8
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | G | , | C | A | G | , | A | C | A | , | T | A | C | , | A | T | G | – | | – | A | G | C | , | G | T | C | , | T | G | T | , | A | T | G | , | T | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 6164_294d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | G | , | T | C | A | , | G | G | T | , | G | C | C | , | T | A | A | – | | – | C | C | C | , | A | G | T | , | C | C | A | , | C | G | G | , | A | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 990a_eafe
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | C | , | A | G | T | , | T | C | C | , | A | T | G | , | A | C | G | – | | – | A | C | G | , | T | C | A | , | A | G | G | , | T | A | C | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC b808_76a9
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | T | , | G | A | A | , | T | G | C | , | G | T | T | , | G | C | A | – | | – | C | G | A | , | C | T | T | , | A | C | G | , | C | A | A | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 568c_3652
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | T | , | C | A | G | , | G | A | C | , | G | T | C | , | A | C | A | – | | – | C | A | A | , | G | T | C | , | C | T | G | , | C | A | G | , | T | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6c6e_3ddb
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | A | , | C | T | G | , | A | G | A | , | G | G | T | , | C | A | G | – | | – | A | T | T | , | G | A | C | , | T | C | T | , | C | C | A | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 2349_2d93
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | G | , | C | G | A | , | G | A | A | , | G | C | T | , | C | A | T | – | | – | C | A | C | , | G | C | T | , | C | T | T | , | C | G | A | , | G | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC fbce_a89a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | T | G | T | , | C | T | G | , | T | T | T | , | G | C | A | – | | – | G | T | A | , | A | C | A | , | G | A | C | , | A | A | A | , | C | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 5084_3c55
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | C | , | A | G | T | , | A | G | T | , | G | T | A | , | C | T | T | – | | – | C | A | G | , | T | C | A | , | T | C | A | , | C | A | T | , | G | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 43b7_562d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | C | T | , | C | A | G | , | C | A | T | , | G | T | C | , | A | G | C | – | | – | A | G | A | , | G | T | C | , | G | T | A | , | C | A | G | , | T | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9fc2_6979
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | T | C | A | , | G | A | G | , | A | A | A | , | C | T | G | – | | – | T | G | C | , | A | G | T | , | C | T | C | , | T | T | T | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC f1a2_ae2c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | G | C | G | , | A | T | C | , | T | A | C | , | G | G | T | – | | – | G | A | T | , | C | G | C | , | T | A | G | , | A | T | G | , | C | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1ce4_2b0b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | C | , | G | G | T | , | A | G | G | , | A | C | G | , | T | T | G | – | | – | T | T | G | , | C | C | A | , | T | C | C | , | T | G | C | , | A | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC f107_1a44
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | A | , | C | G | A | , | T | G | C | , | A | T | T | , | C | T | G | – | | – | A | T | T | , | G | C | T | , | A | C | G | , | T | A | A | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 6dd6_a04e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | A | G | T | , | T | A | C | , | A | G | C | , | T | C | A | – | | – | G | A | T | , | T | C | A | , | A | T | G | , | T | C | G | , | A | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 49e4_1f66
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | C | , | A | A | G | , | A | T | C | , | A | T | T | , | C | A | G | – | | – | A | T | G | , | T | T | C | , | T | A | G | , | T | A | A | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 290f_692b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | A | , | C | G | T | , | G | G | T | , | T | G | T | , | A | C | G | – | | – | A | C | T | , | G | C | A | , | C | C | A | , | A | C | A | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 20e7_4285
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | G | T | C | , | T | G | G | , | T | A | C | , | A | C | G | – | | – | T | G | C | , | C | A | G | , | A | C | C | , | A | T | G | , | T | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 07f7_8de9
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | G | T | C | , | A | G | G | , | A | C | G | , | T | C | G | – | | – | T | G | C | , | C | A | G | , | T | C | C | , | T | G | C | , | A | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC ab08_b281
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | G | , | T | A | T | , | G | A | G | , | T | C | G | , | A | A | C | – | | – | T | G | C | , | A | T | A | , | C | T | C | , | A | G | C | , | T | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5ace_e477
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | A | , | C | T | T | , | G | G | A | , | G | T | T | , | C | A | G | – | | – | A | C | T | , | G | A | A | , | C | C | T | , | C | A | A | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 903b_4e9e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | A | , | G | C | T | , | G | G | A | , | A | T | G | , | A | A | C | – | | – | A | C | T | , | C | G | A | , | C | C | T | , | T | A | C | , | T | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC ec10_e7b2
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | G | , | A | G | T | , | A | T | G | , | C | C | A | , | G | G | T | – | | – | G | T | C | , | T | C | A | , | T | A | C | , | G | G | T | , | C | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 9d0c_c83b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | G | , | C | C | A | , | A | G | T | , | G | A | C | , | T | C | C | – | | – | A | A | C | , | G | G | T | , | T | C | A | , | C | T | G | , | A | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 08e1_d7f3
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | A | , | T | G | A | , | G | T | G | , | C | C | T | , | T | G | A | – | | – | C | G | T | , | A | C | T | , | C | A | C | , | G | G | A | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9d92_d321
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | A | T | A | , | C | A | C | , | A | T | C | , | C | T | G | – | | – | T | C | G | , | T | A | T | , | G | T | G | , | T | A | G | , | G | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC fde3_9f22
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | G | T | G | , | A | A | C | , | G | A | G | , | C | G | T | – | | – | G | T | A | , | C | A | C | , | T | T | G | , | C | T | C | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5dd7_ddb2
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | A | , | T | A | G | , | A | G | C | , | A | C | G | , | T | T | T | – | | – | C | G | T | , | A | T | C | , | T | C | G | , | T | G | C | , | A | A | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 1155_0bf0
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | C | , | A | C | T | , | T | A | C | , | T | C | G | , | T | T | A | – | | – | G | C | G | , | T | G | A | , | A | T | G | , | A | G | C | , | A | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC dde1_c03c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | G | , | A | C | G | , | A | T | T | , | G | T | A | , | T | C | C | – | | – | A | T | C | , | T | G | C | , | T | A | A | , | C | A | T | , | A | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 8c03_fe9d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | A | T | G | , | T | A | G | , | C | G | T | , | C | G | A | – | | – | G | A | C | , | T | A | C | , | A | T | C | , | G | C | A | , | G | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 39dc_62e4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | T | , | G | G | C | , | T | G | C | , | C | A | T | , | T | G | C | – | | – | T | A | A | , | C | C | G | , | A | C | G | , | G | T | A | , | A | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2696_ecd1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | A | , | C | A | G | , | A | C | A | , | T | A | C | , | A | A | G | – | | – | A | C | T | , | G | T | C | , | T | G | T | , | A | T | G | , | T | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2bc2_a805
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | G | , | A | A | C | , | T | T | G | , | C | G | T | , | T | A | G | – | | – | A | C | C | , | T | T | G | , | A | A | C | , | G | C | A | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 54bd_4264
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | G | , | C | A | C | , | G | C | T | , | A | T | C | , | A | G | G | – | | – | A | T | C | , | G | T | G | , | C | G | A | , | T | A | G | , | T | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 2618_bdf6
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | A | , | T | C | T | , | C | A | A | , | C | T | A | , | C | G | T | – | | – | C | A | T | , | A | G | A | , | G | T | T | , | G | A | T | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 7d57_dc82
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | G | , | T | C | T | , | C | A | G | , | C | C | T | , | A | A | G | – | | – | T | C | C | , | A | G | A | , | G | T | C | , | G | G | A | , | T | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC bad0_46e1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | A | , | G | T | C | , | G | T | C | , | C | T | A | , | G | A | C | – | | – | T | A | T | , | C | A | G | , | C | A | G | , | G | A | T | , | C | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ff27_cc2b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | T | , | G | T | A | , | T | C | A | , | A | C | G | , | T | C | G | – | | – | T | G | A | , | C | A | T | , | A | G | T | , | T | G | C | , | A | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 366a_02a2
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | T | , | A | T | G | , | T | G | A | , | G | T | C | , | A | C | T | – | | – | G | C | A | , | T | A | C | , | A | C | T | , | C | A | G | , | T | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 2e9c_72ab
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | A | , | A | C | T | , | A | G | G | , | A | A | G | , | T | C | A | – | | – | C | T | T | , | T | G | A | , | T | C | C | , | T | T | C | , | A | G | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC bb10_e04b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | T | , | C | A | C | , | G | A | C | , | T | G | A | , | C | A | A | – | | – | C | G | A | , | G | T | G | , | C | T | G | , | A | C | T | , | G | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC f610_2964
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | A | , | C | G | T | , | T | G | C | , | G | T | C | , | C | A | A | – | | – | C | T | T | , | G | C | A | , | A | C | G | , | C | A | G | , | G | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC d52f_4f9f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | C | , | C | G | A | , | T | T | C | , | T | G | T | , | A | G | C | – | | – | A | A | G | , | G | C | T | , | A | A | G | , | A | C | A | , | T | C | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c028_cd03
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | C | A | G | , | C | A | C | , | G | G | T | , | A | A | C | – | | – | T | C | A | , | G | T | C | , | G | T | G | , | C | C | A | , | T | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 6989_340e
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | T | , | C | A | G | , | G | A | A | , | C | G | A | , | T | A | C | – | | – | A | A | A | , | G | T | C | , | C | T | T | , | G | C | T | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3e54_ab04
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | T | A | , | G | C | A | , | C | G | T | , | A | T | G | , | A | C | C | – | | – | A | A | T | , | C | G | T | , | G | C | A | , | T | A | C | , | T | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5ec6_6596
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | A | C | , | C | G | T | , | G | G | G | , | T | G | C | , | T | A | G | – | | – | A | T | G | , | G | C | A | , | C | C | C | , | A | C | G | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 968e_52cf
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | G | , | G | C | T | , | C | A | G | , | A | A | G | , | T | A | C | – | | – | T | A | C | , | C | G | A | , | G | T | C | , | T | T | C | , | A | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ec63_ab62
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | A | , | T | C | G | , | T | T | G | , | C | G | T | , | C | A | C | – | | – | T | G | T | , | A | G | C | , | A | A | C | , | G | C | A | , | G | T | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 5217_b7ac
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | C | , | A | T | C | , | T | T | A | , | C | C | A | , | G | T | A | – | | – | C | G | G | , | T | A | G | , | A | A | T | , | G | G | T | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 0d8f_782a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | C | , | T | G | G | , | A | T | C | , | T | C | G | , | T | A | A | – | | – | G | T | G | , | A | C | C | , | T | A | G | , | A | G | C | , | A | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 37bc_3e5f
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | G | , | C | T | A | , | C | C | A | , | C | A | G | , | T | G | G | – | | – | T | C | C | , | G | A | T | , | G | G | T | , | G | T | C | , | A | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC afb3_6212
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | C | , | A | T | G | , | T | T | C | , | T | C | G | , | G | A | G | – | | – | T | T | G | , | T | A | C | , | A | A | G | , | A | G | C | , | C | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 6969_19b7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | G | C | , | T | C | A | , | C | T | T | , | G | A | C | , | T | A | A | – | | – | C | C | G | , | A | G | T | , | G | A | A | , | C | T | G | , | A | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 1cbf_f3bf
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | A | G | T | , | G | C | A | , | A | A | C | , | T | G | T | – | | – | C | T | G | , | T | C | A | , | C | G | T | , | T | T | G | , | A | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9775_88d9
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | T | , | G | A | C | , | A | C | C | , | G | T | A | , | C | T | A | – | | – | G | T | A | , | C | T | G | , | T | G | G | , | C | A | T | , | G | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC d1cf_5a1a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | C | , | A | G | T | , | G | A | G | , | C | G | T | , | T | G | A | – | | – | C | G | G | , | T | C | A | , | C | T | C | , | G | C | A | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 31b6_4d62
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | C | , | G | T | G | , | C | C | A | , | A | G | T | , | C | A | G | – | | – | T | C | G | , | C | A | C | , | G | G | T | , | T | C | A | , | G | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC cdbc_3bd3
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | G | , | C | C | T | , | T | G | T | , | C | T | A | , | G | C | G | – | | – | T | T | C | , | G | G | A | , | A | C | A | , | G | A | T | , | C | G | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 3747_41da
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | A | , | G | T | C | , | C | A | T | , | T | C | T | , | A | T | G | – | | – | T | A | T | , | C | A | G | , | G | T | A | , | A | G | A | , | T | A | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 89c2_832b
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | T | , | A | C | A | , | T | G | C | , | A | A | T | , | C | G | A | – | | – | C | T | A | , | T | G | T | , | A | C | G | , | T | T | A | , | G | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 3b40_4c1d
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | C | A | , | G | T | G | , | G | G | A | , | C | T | G | , | A | G | T | – | | – | G | G | T | , | C | A | C | , | C | C | T | , | G | A | C | , | T | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC cf74_a2bb
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | T | C | , | T | G | A | , | T | G | C | , | A | C | C | , | T | A | G | – | | – | T | A | G | , | A | C | T | , | A | C | G | , | T | G | G | , | A | T | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3d38_9884
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | C | , | T | A | C | , | A | C | G | , | G | T | G | , | A | C | C | – | | – | A | C | G | , | A | T | G | , | T | G | C | , | C | A | C | , | T | G | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC ca1c_0df7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | T | C | , | A | C | A | , | T | A | G | , | C | T | G | , | G | T | A | – | | – | C | A | G | , | T | G | T | , | A | T | C | , | G | A | C | , | C | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC cca1_4484
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | A | , | C | G | T | , | G | T | T | , | G | A | C | , | T | G | A | – | | – | C | G | T | , | G | C | A | , | C | A | A | , | C | T | G | , | A | C | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 45ec_dbf7
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | T | G | G | , | A | C | T | , | A | G | T | , | A | G | C | , | T | T | C | – | | – | A | C | C | , | T | G | A | , | T | C | A | , | T | C | G | , | A | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 7a10_22f4
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | C | , | T | G | T | , | C | G | T | , | A | A | C | , | G | C | T | – | | – | C | T | G | , | A | C | A | , | G | C | A | , | T | T | G | , | C | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9564_d610
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | A | G | , | T | A | C | , | A | G | T | , | T | G | C | , | T | A | T | – | | – | C | T | C | , | A | T | G | , | T | C | A | , | A | C | G | , | A | T | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 2760_d0d1
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | C | T | , | C | G | T | , | T | C | G | , | T | G | C | , | A | T | C | – | | – | T | G | A | , | G | C | A | , | A | G | C | , | A | C | G | , | T | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC caa5_2a0c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | G | T | , | C | C | T | , | A | T | G | , | C | T | A | , | T | G | G | – | | – | T | C | A | , | G | G | A | , | T | A | C | , | G | A | T | , | A | C | C | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7797_b035
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | G | C | , | T | G | A | , | G | G | A | , | T | C | G | , | A | C | T | – | | – | G | C | G | , | A | C | T | , | C | C | T | , | A | G | C | , | T | G | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 89bd_a3a3
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | C | , | G | A | A | , | C | A | C | , | G | G | A | , | C | G | T | – | | – | G | A | G | , | C | T | T | , | G | T | G | , | C | C | T | , | G | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC eb2a_748a
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | A | C | , | G | C | T | , | G | C | C | , | A | C | G | , | T | T | A | – | | – | G | T | G | , | C | G | A | , | C | G | G | , | T | G | C | , | A | A | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c975_7f4c
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | A | , | T | C | G | , | C | A | G | , | G | C | A | , | A | G | T | – | | – | G | A | T | , | A | G | C | , | G | T | C | , | C | G | T | , | T | C | A | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a464_eaac
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | A | A | C | , | A | T | G | , | C | G | A | , | G | C | A | , | A | T | C | – | | – | T | T | G | , | T | A | C | , | G | C | T | , | C | G | T | , | T | A | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC b81c_bbda
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | C | T | , | C | A | T | , | C | G | C | , | T | C | C | , | G | A | A | – | | – | C | G | A | , | G | T | A | , | G | C | G | , | A | G | G | , | C | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 9ec9_3094
EcoR1
overhang | left
unknown
sequence | central
known
sequence | right
unknown
sequence | EcoR1
overhang |
| 5′– | | A | A | , | T | T | C | – | | 3′– | | | | , | | | G | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | C | T | G | , | T | A | A | , | C | T | A | , | G | G | T | , | C | A | A | – | | – | G | A | C | , | A | T | T | , | G | A | T | , | C | C | A | , | G | T | T | – | | | – | N | N | N | , | N | N | N | , | N | N | N | – | | – | N | N | N | , | N | N | N | , | N | N | N | – | | | – | G | | | , | | | | –3′ | | – | C | T | T | , | A | A | | –5′ | |
| | | | | |
Choose the correct pair of RNA primers that will amplify the both the known and unknown region of DNA shown above using inverse PCR.
The RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect