MA

5fb3_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. KpnI is derived from Klebsiella pneumoniae OK8, and NarI is derived from Nocardia argentinensis. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3 4KpnI 5NarI 6KpnI 7 8NarI 9KpnI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with KpnI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

e64b_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. AclI is derived from Acinetobacter calcoaceticus M4, and MluI is derived from Micrococcus luteus. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3 4AclI 5MluI 6 7AclI 8MluI 9 10AclI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AclI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

4bdf_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. HpaI is derived from Haemophilus parainfluenzae, and AanI is derived from Arthrobacter aurescens RFL2. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0HpaI 1 2AanI 3 4HpaI 5 6AanI 7 8 9 10HpaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with HpaI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

ef1d_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. PabI is derived from Pyrococcus abyssi, and NaeI is derived from Nocardia aerocolonigenes. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3PabI 4 5NaeI 6 7 8PabI 9NaeI 10PabI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PabI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

23c2_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. XhoI is derived from Xanthomonas holcicola, and SciI is derived from Streptoverticillium cinnamoneum. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0XhoI 1SciI 2 3 4 5XhoI 6 7 8SciI 9XhoI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with XhoI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

538c_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SacI is derived from Streptomyces achromogenes, and CfoI is derived from Clostridium formicoaceticum. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3 4SacI 5CfoI 6 7SacI 8 9CfoI 10SacI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SacI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

75fd_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. KpnI is derived from Klebsiella pneumoniae OK8, and SgsI is derived from Streptomyces griseus RFL5. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0KpnI 1 2SgsI 3 4KpnI 5 6 7 8 9SgsI 10KpnI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with KpnI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

8d03_3635

Restriction enzymes are proteins that cut DNA at specific sequences. TaiI is derived from Thermus aquaticus cs1-331, and NotI is derived from Nocardia otitidis-caviarum. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0TaiI 1 2 3NotI 4TaiI 5NotI 6 7TaiI 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with TaiI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

f90d_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. ZraI is derived from Zoogloea ramigera 11, and FspI is derived from Fischerella species. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0ZraI 1FspI 2 3ZraI 4 5 6 7FspI 8 9 10ZraI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ZraI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

bb21_3635

Restriction enzymes are proteins that cut DNA at specific sequences. RsaI is derived from Rhodopseudomonas sphaeroides, and SfuI is derived from Streptomyces fulvissimus. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0RsaI 1SfuI 2 3RsaI 4 5SfuI 6 7RsaI 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with RsaI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

0ec0_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SrfI is derived from Streptomyces species Srf, and FseI is derived from Frankia species Eul1b. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0SrfI 1 2 3FseI 4 5SrfI 6 7 8FseI 9SrfI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SrfI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

5df9_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. PacI is derived from Pseudomonas alcaligenes, and AanI is derived from Arthrobacter aurescens RFL2. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0PacI 1 2 3 4AanI 5 6 7PacI 8AanI 9 10PacI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PacI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

7b17_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. PaeI is derived from Pseudomonas aeruginosa, and MscI is derived from Micrococcus species. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1PaeI 2 3 4MscI 5 6 7PaeI 8MscI 9PaeI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PaeI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

dfa1_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. NdeI is derived from Neisseria denitrificans, and SstI is derived from Streptomyces stanford. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0NdeI 1 2 3SstI 4 5 6NdeI 7SstI 8 9 10NdeI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NdeI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

00b3_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. XbaI is derived from Xanthomonas badrii, and SrfI is derived from Streptomyces species Srf. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0XbaI 1 2 3 4SrfI 5XbaI 6 7 8 9SrfI 10XbaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with XbaI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

bf87_3635

Restriction enzymes are proteins that cut DNA at specific sequences. XhoI is derived from Xanthomonas holcicola, and SdaI is derived from Streptomyces diastaticus Ng7-324. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3XhoI 4SdaI 5 6XhoI 7 8 9SdaI 10XhoI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with XhoI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

7b95_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. EgeI is derived from Enterobacter gergoviae NA, and SrfI is derived from Streptomyces species Srf. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0EgeI 1 2SrfI 3 4 5EgeI 6 7 8SrfI 9EgeI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with EgeI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

c2fd_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. MroI is derived from Micrococcus roseus, and SgfI is derived from Streptomyces griseoruber. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0MroI 1 2 3SgfI 4 5MroI 6 7SgfI 8MroI 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MroI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

c615_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. SacI is derived from Streptomyces achromogenes, and EheI is derived from Erwinia herbicola 9/5. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0SacI 1 2EheI 3 4 5SacI 6 7EheI 8 9 10SacI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SacI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

6597_3635

Restriction enzymes are proteins that cut DNA at specific sequences. AgeI is derived from Agrobacterium gelatinovorum, and XmaI is derived from Xanthomonas malvacearum. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2AgeI 3XmaI 4 5 6AgeI 7 8 9XmaI 10AgeI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AgeI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

7916_3635

Restriction enzymes are proteins that cut DNA at specific sequences. PmlI is derived from Pseudomonas maltophilia, and VspI is derived from Vibrio species 343. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3PmlI 4VspI 5PmlI 6 7 8VspI 9PmlI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PmlI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Incorrect MA

6a53_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. StuI is derived from Streptomyces tubercidicus, and AfeI is derived from Alcaligenes faecalis T2774. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2StuI 3AfeI 4StuI 5AfeI 6 7 8 9 10StuI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with StuI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

262a_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. BfaI is derived from Bacteroides fragilis, and PmlI is derived from Pseudomonas maltophilia. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1BfaI 2 3 4PmlI 5BfaI 6 7 8PmlI 9 10BfaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BfaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

4d43_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. NarI is derived from Nocardia argentinensis, and RruI is derived from Rhodococcus ruber RFL5. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0NarI 1 2RruI 3 4NarI 5 6RruI 7 8 9NarI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NarI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

f66b_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. NruI is derived from Nocardia rubra ATCC 15906, and PteI is derived from Paenibacillus terrae RFL2. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0NruI 1 2PteI 3 4 5 6NruI 7 8PteI 9NruI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NruI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

ec30_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. NdeI is derived from Neisseria denitrificans, and KspI is derived from Kluyvera species. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0NdeI 1 2 3KspI 4 5 6NdeI 7KspI 8 9 10NdeI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NdeI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

0885_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. DraI is derived from Deinococcus radiophilus, and SalI is derived from Streptomyces albus G. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0DraI 1SalI 2DraI 3SalI 4 5 6 7DraI 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with DraI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

70f8_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. AanI is derived from Arthrobacter aurescens RFL2, and FspI is derived from Fischerella species. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0AanI 1FspI 2 3AanI 4 5FspI 6 7 8 9AanI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AanI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

cb35_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. HpaI is derived from Haemophilus parainfluenzae, and BfrI is derived from Bacteroides fragilis. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3HpaI 4 5 6 7BfrI 8HpaI 9BfrI 10HpaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with HpaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

f808_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. BfaI is derived from Bacteroides fragilis, and HpaI is derived from Haemophilus parainfluenzae. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1BfaI 2 3HpaI 4BfaI 5HpaI 6 7 8 9 10BfaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BfaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

7960_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. BclI is derived from Bacillus caldolyticus, and PstI is derived from Providencia stuartii 164. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0BclI 1 2PstI 3 4 5BclI 6 7 8 9PstI 10BclI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BclI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

3f1a_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. AscI is derived from Arthrobacter species, and BlnI is derived from Brevibacterium linens. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0AscI 1BlnI 2 3 4AscI 5 6 7 8 9BlnI 10AscI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with AscI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

bc2e_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. PteI is derived from Paenibacillus terrae RFL2, and AfaI is derived from Acidiphilium facilis 28H. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0PteI 1 2AfaI 3 4PteI 5 6 7 8AfaI 9 10PteI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PteI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Incorrect 4 kb Correct 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

cfce_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. SrfI is derived from Streptomyces species Srf, and ZraI is derived from Zoogloea ramigera 11. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0SrfI 1ZraI 2SrfI 3 4 5 6ZraI 7 8SrfI 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SrfI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

5ca1_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. ApaI is derived from Acetobacter pasteurianus sub. pasteurianus, and SmaI is derived from Serratia marcescens Sb. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3 4 5 6ApaI 7SmaI 8ApaI 9SmaI 10ApaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with ApaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

4d9d_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. MreI is derived from Micromonospora rhodorangea RFL1, and NheI is derived from Neisseria mucosa. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0MreI 1 2NheI 3MreI 4 5 6 7 8NheI 9MreI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MreI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

1811_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. NaeI is derived from Nocardia aerocolonigenes, and MlsI is derived from Micrococcus luteus Ng 16-122. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1NaeI 2 3 4MlsI 5 6 7NaeI 8MlsI 9NaeI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with NaeI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

e297_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. MalI is derived from Marinococus albus I, and AhlI is derived from Alteromonas haloplanktis SP. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1MalI 2AhlI 3MalI 4 5AhlI 6MalI 7 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MalI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Correct 6 kb Incorrect MA

09d4_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. BalI is derived from Brevibacterium albidum, and PmeI is derived from Pseudomonas mendocina. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1BalI 2 3PmeI 4 5BalI 6 7PmeI 8 9 10BalI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BalI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

a514_0bb3

Restriction enzymes are proteins that cut DNA at specific sequences. FbaI is derived from Flavobacterium balustinum, and RruI is derived from Rhodococcus ruber RFL5. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1FbaI 2 3 4RruI 5 6 7 8FbaI 9RruI 10FbaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with FbaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Incorrect 7 kb Correct 8 kb Incorrect MA

aae3_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. SdaI is derived from Streptomyces diastaticus Ng7-324, and PdiI is derived from Pseudomonas diminuta Mck 33-321. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1SdaI 2 3PdiI 4SdaI 5PdiI 6 7 8 9 10SdaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SdaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

a8e4_3635

Restriction enzymes are proteins that cut DNA at specific sequences. BcuI is derived from Bacillus coagulans Vs 29-022, and MseI is derived from Micrococcus species. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1BcuI 2 3MseI 4 5BcuI 6 7MseI 8BcuI 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with BcuI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Correct 5 kb Incorrect MA

42bf_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. MfeI is derived from Mycoplasma fermentans, and PacI is derived from Pseudomonas alcaligenes. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1MfeI 2PacI 3MfeI 4 5PacI 6 7 8 9MfeI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MfeI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Incorrect 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

71fe_3635

Restriction enzymes are proteins that cut DNA at specific sequences. MlsI is derived from Micrococcus luteus Ng 16-122, and AseI is derived from Aquaspirillum serpens. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0MlsI 1AseI 2 3 4MlsI 5AseI 6 7MlsI 8 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MlsI only. Select all that apply.
1 kb Correct 2 kb Incorrect 3 kb Correct 4 kb Correct 5 kb Incorrect MA

3011_3635

Restriction enzymes are proteins that cut DNA at specific sequences. PscI is derived from Paracoccus species RFL1, and ClaI is derived from Caryophanon latum L. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2PscI 3ClaI 4 5PscI 6 7ClaI 8 9PscI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with PscI only. Select all that apply.
1 kb Incorrect 2 kb Correct 3 kb Correct 4 kb Correct 5 kb Incorrect MA

4691_d1e8

Restriction enzymes are proteins that cut DNA at specific sequences. DpnI is derived from Diplococcus pneumoniae, and MseI is derived from Micrococcus species. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1 2 3 4DpnI 5MseI 6DpnI 7 8 9MseI 10DpnI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with DpnI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Incorrect 4 kb Correct 5 kb Correct 6 kb Incorrect MA

0fa2_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. MluI is derived from Micrococcus luteus, and BfaI is derived from Bacteroides fragilis. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0MluI 1BfaI 2MluI 3 4 5 6 7BfaI 8MluI 9 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MluI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

54ad_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. SrfI is derived from Streptomyces species Srf, and BfaI is derived from Bacteroides fragilis. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0SrfI 1BfaI 2 3SrfI 4 5 6 7 8BfaI 9SrfI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with SrfI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

9d88_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. MstI is derived from Microcoleus species, and BfaI is derived from Bacteroides fragilis. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0MstI 1BfaI 2 3MstI 4BfaI 5 6 7 8 9MstI 10 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with MstI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect MA

65d2_6a01

Restriction enzymes are proteins that cut DNA at specific sequences. HpaI is derived from Haemophilus parainfluenzae, and NotI is derived from Nocardia otitidis-caviarum. Both enzymes are labeled on the map.

DNA Fragment Question: Shown below is a short DNA fragment that is only 12 kb in length. This fragment has been isolated for restriction enzyme analysis.

  0 1HpaI 2NotI 3 4HpaI 5 6 7 8NotI 9 10HpaI 11 12Scale in kb

Gel interpretation: This is a complete digest. DNA fragments of the same length co-migrate and appear as a single band. Select all distinct band lengths.

Determine the sizes of the DNA bands that would appear on an agarose gel after digestion with HpaI only. Select all that apply.
1 kb Correct 2 kb Correct 3 kb Correct 4 kb Incorrect 5 kb Incorrect 6 kb Correct 7 kb Incorrect