MC cfa4_9a46
| 5′– | A | T | C | , | T | G | C | – | | 3′– | T | A | G | , | A | C | G | – | | | | – | A | G | T | , | C | C | C | , | T | C | G | , | T | G | A | , | C | C | T | , | G | A | G | , | G | T | C | , | A | A | G | – | | – | T | C | A | , | G | G | G | , | A | G | C | , | A | C | T | , | G | G | A | , | C | T | C | , | C | A | G | , | T | T | C | – | | | | – | G | C | C | , | A | G | T | –3′ | | – | C | G | G | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCTGC-3′ and 5′-ACTGGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 6c89_d3f0
| 5′– | C | T | A | , | G | T | T | – | | 3′– | G | A | T | , | C | A | A | – | | | | – | T | G | C | , | C | A | C | , | A | G | A | , | C | A | A | , | C | C | A | , | A | G | C | , | A | T | C | , | T | C | G | – | | – | A | C | G | , | G | T | G | , | T | C | T | , | G | T | T | , | G | G | T | , | T | C | G | , | T | A | G | , | A | G | C | – | | | | – | C | A | T | , | G | G | A | –3′ | | – | G | T | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTAGTT-3′ and 5′-TCCATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 52c4_acf5
| 5′– | G | T | A | , | G | G | T | – | | 3′– | C | A | T | , | C | C | A | – | | | | – | C | G | G | , | A | C | T | , | G | G | C | , | A | T | C | , | G | T | T | , | A | G | A | , | C | T | G | , | A | T | A | – | | – | G | C | C | , | T | G | A | , | C | C | G | , | T | A | G | , | C | A | A | , | T | C | T | , | G | A | C | , | T | A | T | – | | | | – | C | T | T | , | C | G | A | –3′ | | – | G | A | A | , | G | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTAGGT-3′ and 5′-TCGAAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC f960_13f7
| 5′– | C | T | A | , | A | G | T | – | | 3′– | G | A | T | , | T | C | A | – | | | | – | G | G | A | , | C | C | T | , | C | C | T | , | C | C | C | , | A | A | G | , | T | C | T | , | A | A | G | , | T | C | T | – | | – | C | C | T | , | G | G | A | , | G | G | A | , | G | G | G | , | T | T | C | , | A | G | A | , | T | T | C | , | A | G | A | – | | | | – | G | A | A | , | G | A | T | –3′ | | – | C | T | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTAAGT-3′ and 5′-ATCTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 8629_8f95
| 5′– | C | A | T | , | G | A | A | – | | 3′– | G | T | A | , | C | T | T | – | | | | – | T | G | A | , | C | T | A | , | C | G | A | , | G | A | C | , | C | T | G | , | G | A | G | , | T | C | C | , | C | A | G | – | | – | A | C | T | , | G | A | T | , | G | C | T | , | C | T | G | , | G | A | C | , | C | T | C | , | A | G | G | , | G | T | C | – | | | | – | A | G | T | , | C | G | G | –3′ | | – | T | C | A | , | G | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATGAA-3′ and 5′-CCGACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 164f_fd42
| 5′– | T | A | A | , | G | A | C | – | | 3′– | A | T | T | , | C | T | G | – | | | | – | G | C | G | , | T | G | A | , | G | T | A | , | A | G | A | , | T | G | C | , | A | G | A | , | T | T | C | , | A | G | T | – | | – | C | G | C | , | A | C | T | , | C | A | T | , | T | C | T | , | A | C | G | , | T | C | T | , | A | A | G | , | T | C | A | – | | | | – | A | G | T | , | T | G | C | –3′ | | – | T | C | A | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAAGAC-3′ and 5′-GCAACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC e328_0e36
| 5′– | C | T | A | , | T | G | A | – | | 3′– | G | A | T | , | A | C | T | – | | | | – | G | A | T | , | G | C | C | , | A | G | C | , | T | T | C | , | C | A | G | , | G | A | A | , | G | T | A | , | A | C | A | – | | – | C | T | A | , | C | G | G | , | T | C | G | , | A | A | G | , | G | T | C | , | C | T | T | , | C | A | T | , | T | G | T | – | | | | – | C | C | T | , | A | C | T | –3′ | | – | G | G | A | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTATGA-3′ and 5′-AGTAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC efc2_9a24
| 5′– | A | T | C | , | T | T | C | – | | 3′– | T | A | G | , | A | A | G | – | | | | – | T | C | G | , | T | A | A | , | G | A | T | , | C | A | G | , | T | C | G | , | T | G | G | , | T | A | C | , | T | G | G | – | | – | A | G | C | , | A | T | T | , | C | T | A | , | G | T | C | , | A | G | C | , | A | C | C | , | A | T | G | , | A | C | C | – | | | | – | C | A | A | , | A | C | T | –3′ | | – | G | T | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCTTC-3′ and 5′-AGTTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 8e02_7c82
| 5′– | G | A | T | , | A | C | T | – | | 3′– | C | T | A | , | T | G | A | – | | | | – | A | A | T | , | C | A | G | , | G | A | C | , | G | C | T | , | T | G | T | , | A | C | A | , | A | G | C | , | G | T | G | – | | – | T | T | A | , | G | T | C | , | C | T | G | , | C | G | A | , | A | C | A | , | T | G | T | , | T | C | G | , | C | A | C | – | | | | – | G | T | C | , | T | A | A | –3′ | | – | C | A | G | , | A | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATACT-3′ and 5′-TTAGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC ad43_bf3c
| 5′– | T | C | G | , | A | A | C | – | | 3′– | A | G | C | , | T | T | G | – | | | | – | T | G | T | , | C | G | A | , | G | T | T | , | A | G | T | , | G | T | C | , | T | G | A | , | C | A | T | , | A | G | G | – | | – | A | C | A | , | G | C | T | , | C | A | A | , | T | C | A | , | C | A | G | , | A | C | T | , | G | T | A | , | T | C | C | – | | | | – | G | T | A | , | T | G | A | –3′ | | – | C | A | T | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCGAAC-3′ and 5′-TCATAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0b46_fa51
| 5′– | C | T | A | , | G | G | T | – | | 3′– | G | A | T | , | C | C | A | – | | | | – | C | G | A | , | G | C | T | , | G | C | C | , | A | T | C | , | G | G | A | , | C | C | A | , | A | G | C | , | A | T | A | – | | – | G | C | T | , | C | G | A | , | C | G | G | , | T | A | G | , | C | C | T | , | G | G | T | , | T | C | G | , | T | A | T | – | | | | – | T | C | G | , | A | C | C | –3′ | | – | A | G | C | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTAGGT-3′ and 5′-GGTCGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 4781_b864
| 5′– | T | G | C | , | A | G | G | – | | 3′– | A | C | G | , | T | C | C | – | | | | – | A | C | A | , | G | T | A | , | T | G | T | , | T | C | T | , | A | G | G | , | T | C | T | , | A | C | C | , | A | T | G | – | | – | T | G | T | , | C | A | T | , | A | C | A | , | A | G | A | , | T | C | C | , | A | G | A | , | T | G | G | , | T | A | C | – | | | | – | C | C | T | , | C | C | A | –3′ | | – | G | G | A | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCAGG-3′ and 5′-TGGAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 5703_a861
| 5′– | G | C | C | , | T | A | A | – | | 3′– | C | G | G | , | A | T | T | – | | | | – | A | C | C | , | T | G | G | , | G | A | A | , | G | A | G | , | T | A | C | , | A | G | G | , | T | T | C | , | G | A | G | – | | – | T | G | G | , | A | C | C | , | C | T | T | , | C | T | C | , | A | T | G | , | T | C | C | , | A | A | G | , | C | T | C | – | | | | – | A | C | T | , | C | C | G | –3′ | | – | T | G | A | , | G | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCCTAA-3′ and 5′-CGGAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c676_be77
| 5′– | A | G | T | , | T | T | C | – | | 3′– | T | C | A | , | A | A | G | – | | | | – | C | T | A | , | A | G | A | , | T | T | C | , | C | T | G | , | T | G | A | , | T | A | C | , | C | G | T | , | G | A | A | – | | – | G | A | T | , | T | C | T | , | A | A | G | , | G | A | C | , | A | C | T | , | A | T | G | , | G | C | A | , | C | T | T | – | | | | – | C | C | T | , | C | C | A | –3′ | | – | G | G | A | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTTTC-3′ and 5′-TGGAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 7dbb_dfc4
| 5′– | C | T | A | , | G | C | A | – | | 3′– | G | A | T | , | C | G | T | – | | | | – | G | C | G | , | A | T | T | , | C | C | G | , | T | T | C | , | A | G | C | , | A | T | T | , | C | A | T | , | T | G | T | – | | – | C | G | C | , | T | A | A | , | G | G | C | , | A | A | G | , | T | C | G | , | T | A | A | , | G | T | A | , | A | C | A | – | | | | – | A | A | T | , | C | A | G | –3′ | | – | T | T | A | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTAGCA-3′ and 5′-CTGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC ce24_cb93
| 5′– | A | A | C | , | T | C | G | – | | 3′– | T | T | G | , | A | G | C | – | | | | – | T | C | C | , | G | A | G | , | G | A | A | , | T | C | C | , | G | A | C | , | C | C | T | , | T | T | C | , | T | A | G | – | | – | A | G | G | , | C | T | C | , | C | T | T | , | A | G | G | , | C | T | G | , | G | G | A | , | A | A | G | , | A | T | C | – | | | | – | C | T | G | , | G | T | A | –3′ | | – | G | A | C | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACTCG-3′ and 5′-TACCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6ccd_f0c1
| 5′– | T | G | G | , | A | A | G | – | | 3′– | A | C | C | , | T | T | C | – | | | | – | C | T | G | , | A | A | C | , | C | T | T | , | G | A | C | , | G | C | A | , | C | T | A | , | C | T | A | , | C | G | T | – | | – | G | A | C | , | T | T | G | , | G | A | A | , | C | T | G | , | C | G | T | , | G | A | T | , | G | A | T | , | G | C | A | – | | | | – | A | C | G | , | A | T | C | –3′ | | – | T | G | C | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGAAG-3′ and 5′-GATCGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC c872_6373
| 5′– | T | T | G | , | C | A | G | – | | 3′– | A | A | C | , | G | T | C | – | | | | – | G | C | A | , | G | T | A | , | G | G | T | , | G | A | G | , | G | A | C | , | T | A | C | , | G | A | A | , | C | G | T | – | | – | C | G | T | , | C | A | T | , | C | C | A | , | C | T | C | , | C | T | G | , | A | T | G | , | C | T | T | , | G | C | A | – | | | | – | T | C | A | , | T | C | C | –3′ | | – | A | G | T | , | A | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTGCAG-3′ and 5′-GGATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1157_811f
| 5′– | G | G | A | , | G | T | T | – | | 3′– | C | C | T | , | C | A | A | – | | | | – | A | T | C | , | G | G | A | , | T | T | C | , | A | G | T | , | C | C | A | , | C | T | C | , | C | A | T | , | G | T | G | – | | – | T | A | G | , | C | C | T | , | A | A | G | , | T | C | A | , | G | G | T | , | G | A | G | , | G | T | A | , | C | A | C | – | | | | – | C | A | G | , | C | T | A | –3′ | | – | G | T | C | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGAGTT-3′ and 5′-TAGCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1433_e42a
| 5′– | T | A | C | , | A | A | G | – | | 3′– | A | T | G | , | T | T | C | – | | | | – | A | G | A | , | T | G | C | , | A | C | A | , | G | C | T | , | C | C | C | , | T | T | C | , | G | T | C | , | A | G | G | – | | – | T | C | T | , | A | C | G | , | T | G | T | , | C | G | A | , | G | G | G | , | A | A | G | , | C | A | G | , | T | C | C | – | | | | – | G | A | C | , | T | C | A | –3′ | | – | C | T | G | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TACAAG-3′ and 5′-TGAGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 07e4_97bf
| 5′– | G | T | T | , | G | G | A | – | | 3′– | C | A | A | , | C | C | T | – | | | | – | G | C | A | , | T | C | T | , | C | G | A | , | C | C | A | , | T | G | A | , | A | C | T | , | C | C | G | , | T | T | A | – | | – | C | G | T | , | A | G | A | , | G | C | T | , | G | G | T | , | A | C | T | , | T | G | A | , | G | G | C | , | A | A | T | – | | | | – | G | A | C | , | G | G | T | –3′ | | – | C | T | G | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTGGA-3′ and 5′-ACCGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 950a_84df
| 5′– | A | T | C | , | A | A | G | – | | 3′– | T | A | G | , | T | T | C | – | | | | – | T | C | A | , | G | T | T | , | G | C | G | , | T | C | A | , | C | G | G | , | A | C | G | , | A | G | T | , | C | A | G | – | | – | A | G | T | , | C | A | A | , | C | G | C | , | A | G | T | , | G | C | C | , | T | G | C | , | T | C | A | , | G | T | C | – | | | | – | C | G | T | , | T | C | A | –3′ | | – | G | C | A | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCAAG-3′ and 5′-TGAACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC b43c_1e1a
| 5′– | C | C | T | , | C | A | T | – | | 3′– | G | G | A | , | G | T | A | – | | | | – | G | A | G | , | T | A | C | , | A | C | A | , | G | G | A | , | G | T | A | , | G | A | A | , | G | C | T | , | T | G | A | – | | – | C | T | C | , | A | T | G | , | T | G | T | , | C | C | T | , | C | A | T | , | C | T | T | , | C | G | A | , | A | C | T | – | | | | – | A | G | T | , | A | G | G | –3′ | | – | T | C | A | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTCAT-3′ and 5′-CCTACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b9cd_c967
| 5′– | T | A | G | , | G | T | C | – | | 3′– | A | T | C | , | C | A | G | – | | | | – | A | T | G | , | G | C | T | , | T | C | G | , | T | C | T | , | C | A | T | , | C | A | G | , | A | C | G | , | T | C | C | – | | – | T | A | C | , | C | G | A | , | A | G | C | , | A | G | A | , | G | T | A | , | G | T | C | , | T | G | C | , | A | G | G | – | | | | – | G | A | A | , | C | T | A | –3′ | | – | C | T | T | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGGTC-3′ and 5′-TAGTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c249_e7c5
| 5′– | G | C | A | , | G | T | T | – | | 3′– | C | G | T | , | C | A | A | – | | | | – | A | G | A | , | C | C | T | , | C | G | G | , | T | A | C | , | T | C | A | , | A | C | A | , | A | G | A | , | G | T | C | – | | – | T | C | T | , | G | G | A | , | G | C | C | , | A | T | G | , | A | G | T | , | T | G | T | , | T | C | T | , | C | A | G | – | | | | – | T | A | G | , | T | G | G | –3′ | | – | A | T | C | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCAGTT-3′ and 5′-CCACTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 3a1c_d899
| 5′– | G | A | A | , | T | C | A | – | | 3′– | C | T | T | , | A | G | T | – | | | | – | A | G | A | , | G | T | C | , | T | C | A | , | C | A | C | , | C | T | C | , | G | G | T | , | A | C | G | , | T | A | G | – | | – | T | C | T | , | C | A | G | , | A | G | T | , | G | T | G | , | G | A | G | , | C | C | A | , | T | G | C | , | A | T | C | – | | | | – | C | C | G | , | A | C | T | –3′ | | – | G | G | C | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GAATCA-3′ and 5′-AGTCGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 071e_382b
| 5′– | G | A | C | , | C | A | T | – | | 3′– | C | T | G | , | G | T | A | – | | | | – | T | C | T | , | A | C | G | , | C | A | G | , | C | G | T | , | C | A | T | , | C | C | C | , | T | A | C | , | A | G | G | – | | – | A | G | A | , | T | G | C | , | G | T | C | , | G | C | A | , | G | T | A | , | G | G | G | , | A | T | G | , | T | C | C | – | | | | – | C | T | T | , | C | A | T | –3′ | | – | G | A | A | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACCAT-3′ and 5′-ATGAAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 05b3_c775
| 5′– | C | A | T | , | G | G | A | – | | 3′– | G | T | A | , | C | C | T | – | | | | – | A | C | T | , | G | C | A | , | T | G | C | , | A | A | T | , | G | T | G | , | T | T | G | , | C | A | G | , | T | G | C | – | | – | T | G | A | , | C | G | T | , | A | C | G | , | T | T | A | , | C | A | C | , | A | A | C | , | G | T | C | , | A | C | G | – | | | | – | G | T | T | , | C | G | A | –3′ | | – | C | A | A | , | G | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATGGA-3′ and 5′-TCGAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 47a7_34be
| 5′– | G | C | T | , | T | G | A | – | | 3′– | C | G | A | , | A | C | T | – | | | | – | C | A | C | , | A | T | G | , | A | A | T | , | C | C | T | , | C | T | A | , | C | G | C | , | T | C | G | , | A | A | T | – | | – | G | T | G | , | T | A | C | , | T | T | A | , | G | G | A | , | G | A | T | , | G | C | G | , | A | G | C | , | T | T | A | – | | | | – | A | C | T | , | A | C | C | –3′ | | – | T | G | A | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTTGA-3′ and 5′-GGTAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC d628_17b8
| 5′– | G | T | A | , | G | C | T | – | | 3′– | C | A | T | , | C | G | A | – | | | | – | T | T | G | , | A | T | C | , | C | T | T | , | G | C | T | , | C | C | T | , | C | C | T | , | C | A | C | , | T | A | G | – | | – | A | A | C | , | T | A | G | , | G | A | A | , | C | G | A | , | G | G | A | , | G | G | A | , | G | T | G | , | A | T | C | – | | | | – | A | T | T | , | C | G | G | –3′ | | – | T | A | A | , | G | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTAGCT-3′ and 5′-CCGAAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC f5c8_532f
| 5′– | G | G | A | , | C | T | A | – | | 3′– | C | C | T | , | G | A | T | – | | | | – | C | G | A | , | A | T | G | , | G | G | A | , | T | G | T | , | T | C | T | , | A | C | G | , | T | G | C | , | G | A | A | – | | – | G | C | T | , | T | A | C | , | C | C | T | , | A | C | A | , | A | G | A | , | T | G | C | , | A | C | G | , | C | T | T | – | | | | – | T | T | G | , | A | C | C | –3′ | | – | A | A | C | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGACTA-3′ and 5′-GGTCAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC e3fd_a27a
| 5′– | T | G | G | , | A | G | G | – | | 3′– | A | C | C | , | T | C | C | – | | | | – | T | A | C | , | G | G | A | , | A | T | C | , | G | G | A | , | G | C | A | , | T | A | C | , | T | G | T | , | T | A | C | – | | – | A | T | G | , | C | C | T | , | T | A | G | , | C | C | T | , | C | G | T | , | A | T | G | , | A | C | A | , | A | T | G | – | | | | – | G | C | T | , | T | C | A | –3′ | | – | C | G | A | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGAGG-3′ and 5′-TGAAGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC d1f2_643a
| 5′– | T | T | C | , | A | C | G | – | | 3′– | A | A | G | , | T | G | C | – | | | | – | T | G | G | , | A | G | C | , | T | A | C | , | A | A | G | , | G | A | C | , | A | C | G | , | T | T | C | , | G | A | C | – | | – | A | C | C | , | T | C | G | , | A | T | G | , | T | T | C | , | C | T | G | , | T | G | C | , | A | A | G | , | C | T | G | – | | | | – | G | G | T | , | G | G | A | –3′ | | – | C | C | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCACG-3′ and 5′-TCCACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9997_53af
| 5′– | G | A | C | , | A | G | T | – | | 3′– | C | T | G | , | T | C | A | – | | | | – | C | A | C | , | G | A | T | , | T | C | T | , | C | G | T | , | T | G | G | , | A | G | C | , | A | C | G | , | T | A | A | – | | – | G | T | G | , | C | T | A | , | A | G | A | , | G | C | A | , | A | C | C | , | T | C | G | , | T | G | C | , | A | T | T | – | | | | – | G | G | T | , | C | G | A | –3′ | | – | C | C | A | , | G | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACAGT-3′ and 5′-TCGACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 43b1_27ad
| 5′– | C | A | T | , | G | A | A | – | | 3′– | G | T | A | , | C | T | T | – | | | | – | G | A | T | , | A | G | C | , | T | G | A | , | C | T | A | , | C | G | T | , | C | G | T | , | C | T | G | , | A | G | T | – | | – | C | T | A | , | T | C | G | , | A | C | T | , | G | A | T | , | G | C | A | , | G | C | A | , | G | A | C | , | T | C | A | – | | | | – | T | G | A | , | T | T | C | –3′ | | – | A | C | T | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATGAA-3′ and 5′-GAATCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC adf8_663b
| 5′– | T | G | C | , | A | G | G | – | | 3′– | A | C | G | , | T | C | C | – | | | | – | A | C | T | , | T | A | G | , | C | T | C | , | C | T | A | , | A | G | G | , | C | T | A | , | A | G | G | , | T | T | C | – | | – | T | G | A | , | A | T | C | , | G | A | G | , | G | A | T | , | T | C | C | , | G | A | T | , | T | C | C | , | A | A | G | – | | | | – | C | T | G | , | T | C | A | –3′ | | – | G | A | C | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCAGG-3′ and 5′-TGACAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 1e2a_4bc3
| 5′– | T | T | C | , | A | T | C | – | | 3′– | A | A | G | , | T | A | G | – | | | | – | C | A | A | , | T | G | A | , | T | C | T | , | G | T | C | , | T | C | A | , | G | A | T | , | T | G | A | , | G | C | A | – | | – | G | T | T | , | A | C | T | , | A | G | A | , | C | A | G | , | A | G | T | , | C | T | A | , | A | C | T | , | C | G | T | – | | | | – | G | T | A | , | T | C | A | –3′ | | – | C | A | T | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCATC-3′ and 5′-TGATAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC a17c_b3fe
| 5′– | C | T | T | , | C | T | A | – | | 3′– | G | A | A | , | G | A | T | – | | | | – | T | G | G | , | T | C | A | , | C | A | C | , | T | C | C | , | G | A | A | , | C | G | G | , | A | A | C | , | T | A | G | – | | – | A | C | C | , | A | G | T | , | G | T | G | , | A | G | G | , | C | T | T | , | G | C | C | , | T | T | G | , | A | T | C | – | | | | – | C | T | A | , | C | C | T | –3′ | | – | G | A | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTTCTA-3′ and 5′-AGGTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a557_22ad
| 5′– | G | T | T | , | C | G | A | – | | 3′– | C | A | A | , | G | C | T | – | | | | – | T | A | C | , | G | A | C | , | A | A | G | , | T | G | T | , | A | A | G | , | T | C | T | , | G | C | C | , | T | A | C | – | | – | A | T | G | , | C | T | G | , | T | T | C | , | A | C | A | , | T | T | C | , | A | G | A | , | C | G | G | , | A | T | G | – | | | | – | T | T | A | , | G | C | C | –3′ | | – | A | A | T | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTCGA-3′ and 5′-GGCTAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 99c3_d27a
| 5′– | G | A | C | , | G | A | T | – | | 3′– | C | T | G | , | C | T | A | – | | | | – | A | T | C | , | T | C | G | , | T | G | G | , | A | G | G | , | A | C | C | , | T | G | T | , | T | G | C | , | A | G | G | – | | – | T | A | G | , | A | G | C | , | A | C | C | , | T | C | C | , | T | G | G | , | A | C | A | , | A | C | G | , | T | C | C | – | | | | – | G | T | A | , | G | A | A | –3′ | | – | C | A | T | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACGAT-3′ and 5′-TTCTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 01bf_fe4e
| 5′– | G | A | C | , | C | T | A | – | | 3′– | C | T | G | , | G | A | T | – | | | | – | T | C | T | , | C | G | A | , | C | A | C | , | A | C | T | , | A | C | G | , | T | C | C | , | T | A | T | , | G | C | G | – | | – | A | G | A | , | G | C | T | , | G | T | G | , | T | G | A | , | T | G | C | , | A | G | G | , | A | T | A | , | C | G | C | – | | | | – | A | A | C | , | G | T | C | –3′ | | – | T | T | G | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACCTA-3′ and 5′-GACGTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b993_31f2
| 5′– | C | A | G | , | G | C | T | – | | 3′– | G | T | C | , | C | G | A | – | | | | – | C | T | T | , | G | A | G | , | T | T | G | , | G | C | T | , | T | C | G | , | T | C | C | , | A | C | T | , | G | G | A | – | | – | G | A | A | , | C | T | C | , | A | A | C | , | C | G | A | , | A | G | C | , | A | G | G | , | T | G | A | , | C | C | T | – | | | | – | A | T | C | , | A | A | G | –3′ | | – | T | A | G | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGGCT-3′ and 5′-CTTGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9c37_339f
| 5′– | C | C | T | , | G | A | T | – | | 3′– | G | G | A | , | C | T | A | – | | | | – | T | T | C | , | C | A | G | , | C | T | C | , | A | G | T | , | G | A | G | , | G | A | A | , | G | A | G | , | T | G | C | – | | – | A | A | G | , | G | T | C | , | G | A | G | , | T | C | A | , | C | T | C | , | C | T | T | , | C | T | C | , | A | C | G | – | | | | – | G | A | T | , | G | T | A | –3′ | | – | C | T | A | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTGAT-3′ and 5′-TACATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC d813_c2be
| 5′– | A | A | G | , | T | T | G | – | | 3′– | T | T | C | , | A | A | C | – | | | | – | G | G | G | , | T | C | A | , | G | T | G | , | C | T | C | , | T | G | G | , | T | G | A | , | C | A | T | , | G | C | T | – | | – | C | C | C | , | A | G | T | , | C | A | C | , | G | A | G | , | A | C | C | , | A | C | T | , | G | T | A | , | C | G | A | – | | | | – | G | A | T | , | C | T | T | –3′ | | – | C | T | A | , | G | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AAGTTG-3′ and 5′-AAGATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1fd4_a64d
| 5′– | G | T | C | , | T | G | A | – | | 3′– | C | A | G | , | A | C | T | – | | | | – | T | G | C | , | C | A | A | , | G | G | T | , | A | G | G | , | A | C | T | , | T | T | C | , | G | T | G | , | A | T | C | – | | – | A | C | G | , | G | T | T | , | C | C | A | , | T | C | C | , | T | G | A | , | A | A | G | , | C | A | C | , | T | A | G | – | | | | – | C | A | G | , | A | G | T | –3′ | | – | G | T | C | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCTGA-3′ and 5′-ACTCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b563_ac7b
| 5′– | C | T | G | , | C | A | A | – | | 3′– | G | A | C | , | G | T | T | – | | | | – | G | A | A | , | T | C | T | , | G | T | A | , | G | A | G | , | C | A | C | , | G | C | T | , | A | C | G | , | C | T | T | – | | – | C | T | T | , | A | G | A | , | C | A | T | , | C | T | C | , | G | T | G | , | C | G | A | , | T | G | C | , | G | A | A | – | | | | – | C | C | A | , | A | C | T | –3′ | | – | G | G | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGCAA-3′ and 5′-AGTTGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC b4b1_62f6
| 5′– | G | T | T | , | A | C | T | – | | 3′– | C | A | A | , | T | G | A | – | | | | – | G | G | G | , | T | A | C | , | A | A | A | , | C | G | A | , | G | A | A | , | T | G | T | , | A | C | G | , | A | T | A | – | | – | C | C | C | , | A | T | G | , | T | T | T | , | G | C | T | , | C | T | T | , | A | C | A | , | T | G | C | , | T | A | T | – | | | | – | A | C | T | , | A | T | C | –3′ | | – | T | G | A | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTACT-3′ and 5′-GATAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2b84_6a4f
| 5′– | A | T | G | , | T | G | G | – | | 3′– | T | A | C | , | A | C | C | – | | | | – | C | G | A | , | T | T | C | , | A | C | C | , | T | G | T | , | A | A | G | , | A | T | G | , | A | G | T | , | C | G | A | – | | – | G | C | T | , | A | A | G | , | T | G | G | , | A | C | A | , | T | T | C | , | T | A | C | , | T | C | A | , | G | C | T | – | | | | – | C | G | G | , | A | G | T | –3′ | | – | G | C | C | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGTGG-3′ and 5′-ACTCCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 47a5_999f
| 5′– | G | T | T | , | A | C | T | – | | 3′– | C | A | A | , | T | G | A | – | | | | – | C | A | T | , | C | G | A | , | G | A | G | , | T | C | T | , | G | A | T | , | A | C | T | , | C | A | A | , | G | A | T | – | | – | G | T | A | , | G | C | T | , | C | T | C | , | A | G | A | , | C | T | A | , | T | G | A | , | G | T | T | , | C | T | A | – | | | | – | T | C | A | , | C | T | G | –3′ | | – | A | G | T | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTACT-3′ and 5′-CAGTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 5334_2956
| 5′– | T | G | G | , | G | A | C | – | | 3′– | A | C | C | , | C | T | G | – | | | | – | G | G | C | , | T | A | A | , | C | G | G | , | A | T | G | , | G | T | T | , | C | A | G | , | A | G | C | , | T | A | T | – | | – | C | C | G | , | A | T | T | , | G | C | C | , | T | A | C | , | C | A | A | , | G | T | C | , | T | C | G | , | A | T | A | – | | | | – | C | A | A | , | G | G | T | –3′ | | – | G | T | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGGAC-3′ and 5′-ACCTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC c636_f65b
| 5′– | C | G | G | , | A | G | T | – | | 3′– | G | C | C | , | T | C | A | – | | | | – | T | C | G | , | A | T | A | , | G | A | C | , | A | G | T | , | G | A | T | , | C | T | C | , | C | G | A | , | A | T | G | – | | – | A | G | C | , | T | A | T | , | C | T | G | , | T | C | A | , | C | T | A | , | G | A | G | , | G | C | T | , | T | A | C | – | | | | – | C | A | A | , | C | G | T | –3′ | | – | G | T | T | , | G | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGGAGT-3′ and 5′-ACGTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7d50_a01e
| 5′– | A | T | G | , | T | T | C | – | | 3′– | T | A | C | , | A | A | G | – | | | | – | C | G | T | , | C | T | A | , | C | C | C | , | A | T | G | , | T | C | G | , | C | T | C | , | C | T | A | , | G | C | T | – | | – | G | C | A | , | G | A | T | , | G | G | G | , | T | A | C | , | A | G | C | , | G | A | G | , | G | A | T | , | C | G | A | – | | | | – | C | A | G | , | G | T | A | –3′ | | – | G | T | C | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGTTC-3′ and 5′-TACCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 84a4_5029
| 5′– | T | G | A | , | T | G | G | – | | 3′– | A | C | T | , | A | C | C | – | | | | – | T | A | A | , | G | C | T | , | C | A | A | , | C | G | T | , | C | G | T | , | C | T | T | , | G | T | T | , | G | A | C | – | | – | A | T | T | , | C | G | A | , | G | T | T | , | G | C | A | , | G | C | A | , | G | A | A | , | C | A | A | , | C | T | G | – | | | | – | G | T | T | , | T | C | A | –3′ | | – | C | A | A | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGATGG-3′ and 5′-TGAAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c44c_865a
| 5′– | T | C | A | , | A | T | C | – | | 3′– | A | G | T | , | T | A | G | – | | | | – | G | G | T | , | C | T | A | , | G | C | A | , | C | G | G | , | A | G | G | , | T | G | G | , | G | A | T | , | C | C | A | – | | – | C | C | A | , | G | A | T | , | C | G | T | , | G | C | C | , | T | C | C | , | A | C | C | , | C | T | A | , | G | G | T | – | | | | – | C | T | G | , | G | A | T | –3′ | | – | G | A | C | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAATC-3′ and 5′-ATCCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 7514_4b0e
| 5′– | A | C | G | , | A | T | G | – | | 3′– | T | G | C | , | T | A | C | – | | | | – | T | G | T | , | G | C | A | , | T | G | G | , | C | T | G | , | G | A | G | , | G | C | A | , | C | T | A | , | C | A | G | – | | – | A | C | A | , | C | G | T | , | A | C | C | , | G | A | C | , | C | T | C | , | C | G | T | , | G | A | T | , | G | T | C | – | | | | – | G | G | A | , | A | C | T | –3′ | | – | C | C | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACGATG-3′ and 5′-AGTTCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8db9_5192
| 5′– | C | T | G | , | G | G | A | – | | 3′– | G | A | C | , | C | C | T | – | | | | – | C | G | G | , | T | T | A | , | G | A | T | , | T | G | C | , | T | C | C | , | C | A | A | , | C | C | C | , | A | G | T | – | | – | G | C | C | , | A | A | T | , | C | T | A | , | A | C | G | , | A | G | G | , | G | T | T | , | G | G | G | , | T | C | A | – | | | | – | C | A | A | , | C | T | A | –3′ | | – | G | T | T | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGGGA-3′ and 5′-TAGTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7de7_46d9
| 5′– | T | T | C | , | C | A | G | – | | 3′– | A | A | G | , | G | T | C | – | | | | – | C | G | A | , | C | T | A | , | C | G | G | , | A | C | A | , | C | G | A | , | A | T | G | , | C | T | G | , | T | T | A | – | | – | G | C | T | , | G | A | T | , | G | C | C | , | T | G | T | , | G | C | T | , | T | A | C | , | G | A | C | , | A | A | T | – | | | | – | C | T | G | , | T | T | A | –3′ | | – | G | A | C | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCCAG-3′ and 5′-TAACAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 75f7_3a99
| 5′– | G | A | T | , | C | G | A | – | | 3′– | C | T | A | , | G | C | T | – | | | | – | A | C | T | , | G | A | C | , | T | A | C | , | C | A | G | , | G | T | C | , | G | A | A | , | T | G | T | , | A | A | C | – | | – | T | G | A | , | C | T | G | , | A | T | G | , | G | T | C | , | C | A | G | , | C | T | T | , | A | C | A | , | T | T | G | – | | | | – | T | G | C | , | G | A | C | –3′ | | – | A | C | G | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATCGA-3′ and 5′-GTCGCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 863a_e44a
| 5′– | G | G | T | , | G | A | A | – | | 3′– | C | C | A | , | C | T | T | – | | | | – | G | C | C | , | A | G | T | , | A | C | G | , | G | A | T | , | T | C | C | , | A | G | A | , | A | G | C | , | A | T | A | – | | – | C | G | G | , | T | C | A | , | T | G | C | , | C | T | A | , | A | G | G | , | T | C | T | , | T | C | G | , | T | A | T | – | | | | – | T | C | A | , | T | G | C | –3′ | | – | A | G | T | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGTGAA-3′ and 5′-GCATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 5c7f_65a5
| 5′– | T | A | A | , | C | T | G | – | | 3′– | A | T | T | , | G | A | C | – | | | | – | G | A | C | , | T | T | C | , | T | T | A | , | C | C | A | , | G | A | T | , | C | C | G | , | T | G | T | , | C | G | A | – | | – | C | T | G | , | A | A | G | , | A | A | T | , | G | G | T | , | C | T | A | , | G | G | C | , | A | C | A | , | G | C | T | – | | | | – | G | C | A | , | G | G | T | –3′ | | – | C | G | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAACTG-3′ and 5′-ACCTGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC bd17_ca70
| 5′– | A | T | C | , | G | T | C | – | | 3′– | T | A | G | , | C | A | G | – | | | | – | T | G | C | , | C | A | G | , | T | C | C | , | G | A | T | , | C | A | C | , | G | C | T | , | T | G | A | , | T | T | C | – | | – | A | C | G | , | G | T | C | , | A | G | G | , | C | T | A | , | G | T | G | , | C | G | A | , | A | C | T | , | A | A | G | – | | | | – | C | C | T | , | G | A | A | –3′ | | – | G | G | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCGTC-3′ and 5′-TTCAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 48e7_83fe
| 5′– | C | C | A | , | C | C | T | – | | 3′– | G | G | T | , | G | G | A | – | | | | – | G | C | A | , | T | A | G | , | C | C | A | , | C | C | T | , | T | T | C | , | G | G | A | , | A | C | T | , | C | G | A | – | | – | C | G | T | , | A | T | C | , | G | G | T | , | G | G | A | , | A | A | G | , | C | C | T | , | T | G | A | , | G | C | T | – | | | | – | C | C | A | , | A | C | T | –3′ | | – | G | G | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCACCT-3′ and 5′-AGTTGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 579a_f780
| 5′– | C | C | A | , | C | A | T | – | | 3′– | G | G | T | , | G | T | A | – | | | | – | T | A | C | , | G | A | C | , | C | A | T | , | G | C | A | , | A | C | C | , | G | A | G | , | T | A | T | , | C | G | C | – | | – | A | T | G | , | C | T | G | , | G | T | A | , | C | G | T | , | T | G | G | , | C | T | C | , | A | T | A | , | G | C | G | – | | | | – | T | T | A | , | G | T | C | –3′ | | – | A | A | T | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCACAT-3′ and 5′-GACTAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC eceb_2e9b
| 5′– | A | G | T | , | A | G | G | – | | 3′– | T | C | A | , | T | C | C | – | | | | – | C | A | G | , | C | G | T | , | G | T | A | , | A | C | G | , | G | A | A | , | T | G | T | , | A | G | G | , | T | C | T | – | | – | G | T | C | , | G | C | A | , | C | A | T | , | T | G | C | , | C | T | T | , | A | C | A | , | T | C | C | , | A | G | A | – | | | | – | G | A | C | , | G | T | T | –3′ | | – | C | T | G | , | C | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTAGG-3′ and 5′-AACGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 7dff_5098
| 5′– | C | C | A | , | T | C | A | – | | 3′– | G | G | T | , | A | G | T | – | | | | – | T | C | G | , | G | A | G | , | T | T | G | , | A | T | T | , | G | C | T | , | G | A | C | , | C | T | A | , | C | C | G | – | | – | A | G | C | , | C | T | C | , | A | A | C | , | T | A | A | , | C | G | A | , | C | T | G | , | G | A | T | , | G | G | C | – | | | | – | G | T | C | , | A | C | T | –3′ | | – | C | A | G | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCATCA-3′ and 5′-AGTGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC c0a0_cd5c
| 5′– | T | A | G | , | T | G | G | – | | 3′– | A | T | C | , | A | C | C | – | | | | – | A | T | C | , | A | T | G | , | G | A | G | , | A | T | C | , | G | A | C | , | G | A | A | , | T | G | G | , | A | T | C | – | | – | T | A | G | , | T | A | C | , | C | T | C | , | T | A | G | , | C | T | G | , | C | T | T | , | A | C | C | , | T | A | G | – | | | | – | T | G | A | , | G | A | C | –3′ | | – | A | C | T | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGTGG-3′ and 5′-GTCTCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 70b7_af4f
| 5′– | G | C | T | , | T | C | A | – | | 3′– | C | G | A | , | A | G | T | – | | | | – | T | C | G | , | A | T | C | , | T | C | G | , | G | A | A | , | T | C | A | , | T | T | G | , | T | T | G | , | A | C | C | – | | – | A | G | C | , | T | A | G | , | A | G | C | , | C | T | T | , | A | G | T | , | A | A | C | , | A | A | C | , | T | G | G | – | | | | – | G | T | A | , | A | C | T | –3′ | | – | C | A | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTTCA-3′ and 5′-AGTTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC e323_2124
| 5′– | T | G | A | , | A | A | C | – | | 3′– | A | C | T | , | T | T | G | – | | | | – | G | G | A | , | A | C | T | , | T | C | C | , | A | G | T | , | A | C | T | , | A | G | A | , | G | T | A | , | A | C | T | – | | – | C | C | T | , | T | G | A | , | A | G | G | , | T | C | A | , | T | G | A | , | T | C | T | , | C | A | T | , | T | G | A | – | | | | – | C | T | A | , | G | T | A | –3′ | | – | G | A | T | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAAAC-3′ and 5′-TACTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC bde1_ee20
| 5′– | C | A | G | , | C | A | T | – | | 3′– | G | T | C | , | G | T | A | – | | | | – | T | C | G | , | A | C | T | , | C | G | A | , | T | G | C | , | C | T | G | , | C | A | C | , | C | T | T | , | A | C | G | – | | – | A | G | C | , | T | G | A | , | G | C | T | , | A | C | G | , | G | A | C | , | G | T | G | , | G | A | A | , | T | G | C | – | | | | – | G | T | C | , | A | T | T | –3′ | | – | C | A | G | , | T | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGCAT-3′ and 5′-AATGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 6323_a35f
| 5′– | G | T | C | , | G | G | A | – | | 3′– | C | A | G | , | C | C | T | – | | | | – | T | C | T | , | G | A | G | , | G | T | C | , | T | T | C | , | T | G | G | , | A | G | C | , | G | A | A | , | T | C | G | – | | – | A | G | A | , | C | T | C | , | C | A | G | , | A | A | G | , | A | C | C | , | T | C | G | , | C | T | T | , | A | G | C | – | | | | – | C | T | A | , | C | A | T | –3′ | | – | G | A | T | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCGGA-3′ and 5′-ATGTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 4bd4_5459
| 5′– | T | C | G | , | A | C | G | – | | 3′– | A | G | C | , | T | G | C | – | | | | – | G | C | A | , | T | T | G | , | T | C | C | , | C | A | T | , | A | C | C | , | C | A | G | , | G | A | C | , | T | T | T | – | | – | C | G | T | , | A | A | C | , | A | G | G | , | G | T | A | , | T | G | G | , | G | T | C | , | C | T | G | , | A | A | A | – | | | | – | A | G | T | , | A | A | G | –3′ | | – | T | C | A | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCGACG-3′ and 5′-CTTACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 998e_acb8
| 5′– | T | T | G | , | A | A | G | – | | 3′– | A | A | C | , | T | T | C | – | | | | – | C | A | G | , | G | C | T | , | G | G | T | , | A | T | C | , | A | T | C | , | C | A | C | , | A | C | C | , | A | G | T | – | | – | G | T | C | , | C | G | A | , | C | C | A | , | T | A | G | , | T | A | G | , | G | T | G | , | T | G | G | , | T | C | A | – | | | | – | G | A | C | , | A | C | T | –3′ | | – | C | T | G | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTGAAG-3′ and 5′-AGTGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC d774_3601
| 5′– | C | A | T | , | G | C | A | – | | 3′– | G | T | A | , | C | G | T | – | | | | – | T | A | C | , | G | C | T | , | C | T | C | , | C | A | T | , | G | T | G | , | T | C | G | , | C | A | G | , | T | A | C | – | | – | A | T | G | , | C | G | A | , | G | A | G | , | G | T | A | , | C | A | C | , | A | G | C | , | G | T | C | , | A | T | G | – | | | | – | T | T | G | , | A | G | G | –3′ | | – | A | A | C | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATGCA-3′ and 5′-CCTCAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 610c_6138
| 5′– | G | A | T | , | T | C | A | – | | 3′– | C | T | A | , | A | G | T | – | | | | – | T | C | G | , | A | G | A | , | A | G | A | , | T | C | C | , | A | A | G | , | T | G | A | , | G | A | T | , | C | C | C | – | | – | A | G | C | , | T | C | T | , | T | C | T | , | A | G | G | , | T | T | C | , | A | C | T | , | C | T | A | , | G | G | G | – | | | | – | A | T | C | , | G | A | C | –3′ | | – | T | A | G | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATTCA-3′ and 5′-GTCGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4d5b_2420
| 5′– | T | T | C | , | T | A | G | – | | 3′– | A | A | G | , | A | T | C | – | | | | – | G | T | G | , | A | A | C | , | A | G | G | , | A | C | G | , | T | G | G | , | T | C | A | , | G | T | G | , | A | C | A | – | | – | C | A | C | , | T | T | G | , | T | C | C | , | T | G | C | , | A | C | C | , | A | G | T | , | C | A | C | , | T | G | T | – | | | | – | C | T | G | , | C | C | A | –3′ | | – | G | A | C | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCTAG-3′ and 5′-TGGCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 654d_d423
| 5′– | T | G | A | , | A | T | G | – | | 3′– | A | C | T | , | T | A | C | – | | | | – | G | T | A | , | C | G | C | , | A | A | G | , | T | T | A | , | G | G | T | , | A | C | T | , | C | A | G | , | T | T | A | – | | – | C | A | T | , | G | C | G | , | T | T | C | , | A | A | T | , | C | C | A | , | T | G | A | , | G | T | C | , | A | A | T | – | | | | – | C | G | T | , | T | C | A | –3′ | | – | G | C | A | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAATG-3′ and 5′-TGAACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 49e9_4c10
| 5′– | T | A | G | , | A | C | C | – | | 3′– | A | T | C | , | T | G | G | – | | | | – | T | G | A | , | A | T | C | , | C | G | A | , | T | T | G | , | T | C | T | , | C | A | G | , | A | A | C | , | C | T | G | – | | – | A | C | T | , | T | A | G | , | G | C | T | , | A | A | C | , | A | G | A | , | G | T | C | , | T | T | G | , | G | A | C | – | | | | – | G | G | A | , | G | G | T | –3′ | | – | C | C | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGACC-3′ and 5′-ACCTCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 9125_4056
| 5′– | A | G | C | , | T | C | C | – | | 3′– | T | C | G | , | A | G | G | – | | | | – | T | A | C | , | A | G | T | , | G | G | A | , | A | C | T | , | T | C | T | , | T | G | C | , | G | A | C | , | A | T | C | – | | – | A | T | G | , | T | C | A | , | C | C | T | , | T | G | A | , | A | G | A | , | A | C | G | , | C | T | G | , | T | A | G | – | | | | – | C | A | G | , | A | C | T | –3′ | | – | G | T | C | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGCTCC-3′ and 5′-AGTCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 567b_579c
| 5′– | A | T | C | , | G | T | C | – | | 3′– | T | A | G | , | C | A | G | – | | | | – | T | G | A | , | C | G | T | , | G | A | C | , | C | A | G | , | C | A | C | , | C | A | T | , | A | C | C | , | C | T | G | – | | – | A | C | T | , | G | C | A | , | C | T | G | , | G | T | C | , | G | T | G | , | G | T | A | , | T | G | G | , | G | A | C | – | | | | – | T | A | A | , | C | T | G | –3′ | | – | A | T | T | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCGTC-3′ and 5′-CAGTTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4bab_63c8
| 5′– | A | T | C | , | A | G | G | – | | 3′– | T | A | G | , | T | C | C | – | | | | – | G | C | A | , | A | T | G | , | A | T | G | , | A | G | G | , | T | A | G | , | C | T | T | , | C | C | G | , | T | A | A | – | | – | C | G | T | , | T | A | C | , | T | A | C | , | T | C | C | , | A | T | C | , | G | A | A | , | G | G | C | , | A | T | T | – | | | | – | T | T | G | , | C | A | G | –3′ | | – | A | A | C | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCAGG-3′ and 5′-CTGCAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC ec19_f5dc
| 5′– | T | G | C | , | A | G | C | – | | 3′– | A | C | G | , | T | C | G | – | | | | – | G | A | T | , | G | C | C | , | A | C | C | , | C | T | G | , | A | G | T | , | G | T | T | , | C | G | C | , | A | T | A | – | | – | C | T | A | , | C | G | G | , | T | G | G | , | G | A | C | , | T | C | A | , | C | A | A | , | G | C | G | , | T | A | T | – | | | | – | G | C | C | , | A | A | T | –3′ | | – | C | G | G | , | T | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCAGC-3′ and 5′-ATTGGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 92a3_b663
| 5′– | T | G | A | , | T | G | G | – | | 3′– | A | C | T | , | A | C | C | – | | | | – | G | T | C | , | T | A | T | , | C | A | T | , | G | C | A | , | A | T | G | , | C | A | C | , | A | T | T | , | G | C | T | – | | – | C | A | G | , | A | T | A | , | G | T | A | , | C | G | T | , | T | A | C | , | G | T | G | , | T | A | A | , | C | G | A | – | | | | – | C | A | A | , | C | T | A | –3′ | | – | G | T | T | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGATGG-3′ and 5′-TAGTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2b09_3dd4
| 5′– | C | T | G | , | C | A | A | – | | 3′– | G | A | C | , | G | T | T | – | | | | – | A | T | C | , | C | G | T | , | C | T | C | , | C | C | T | , | T | T | G | , | C | T | T | , | T | G | C | , | T | A | C | – | | – | T | A | G | , | G | C | A | , | G | A | G | , | G | G | A | , | A | A | C | , | G | A | A | , | A | C | G | , | A | T | G | – | | | | – | A | A | C | , | T | C | C | –3′ | | – | T | T | G | , | A | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGCAA-3′ and 5′-GGAGTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 069d_7b32
| 5′– | A | A | C | , | A | T | G | – | | 3′– | T | T | G | , | T | A | C | – | | | | – | C | T | A | , | T | C | G | , | T | T | C | , | A | G | A | , | A | C | C | , | G | A | A | , | A | G | T | , | C | T | A | – | | – | G | A | T | , | A | G | C | , | A | A | G | , | T | C | T | , | T | G | G | , | C | T | T | , | T | C | A | , | G | A | T | – | | | | – | C | T | A | , | T | G | A | –3′ | | – | G | A | T | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACATG-3′ and 5′-TCATAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c189_c54f
| 5′– | C | C | A | , | A | C | T | – | | 3′– | G | G | T | , | T | G | A | – | | | | – | C | A | C | , | T | T | G | , | T | C | C | , | T | T | G | , | A | A | C | , | G | A | A | , | A | G | C | , | T | T | A | – | | – | G | T | G | , | A | A | C | , | A | G | G | , | A | A | C | , | T | T | G | , | C | T | T | , | T | C | G | , | A | A | T | – | | | | – | A | A | G | , | C | T | G | –3′ | | – | T | T | C | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCAACT-3′ and 5′-CAGCTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC c737_b2de
| 5′– | A | G | G | , | T | A | G | – | | 3′– | T | C | C | , | A | T | C | – | | | | – | A | C | A | , | G | T | C | , | T | A | T | , | G | T | G | , | A | A | T | , | C | A | A | , | A | G | C | , | T | G | C | – | | – | T | G | T | , | C | A | G | , | A | T | A | , | C | A | C | , | T | T | A | , | G | T | T | , | T | C | G | , | A | C | G | – | | | | – | T | C | A | , | G | C | C | –3′ | | – | A | G | T | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTAG-3′ and 5′-GGCTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c0e0_d0c5
| 5′– | A | A | G | , | G | T | C | – | | 3′– | T | T | C | , | C | A | G | – | | | | – | G | G | T | , | T | C | A | , | C | T | T | , | G | A | T | , | G | A | T | , | C | C | G | , | A | C | T | , | G | T | T | – | | – | C | C | A | , | A | G | T | , | G | A | A | , | C | T | A | , | C | T | A | , | G | G | C | , | T | G | A | , | C | A | A | – | | | | – | C | C | T | , | T | G | A | –3′ | | – | G | G | A | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AAGGTC-3′ and 5′-TCAAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 811d_8fea
| 5′– | C | T | A | , | G | A | A | – | | 3′– | G | A | T | , | C | T | T | – | | | | – | G | G | A | , | T | A | C | , | A | T | G | , | T | G | G | , | G | T | A | , | G | G | G | , | T | C | A | , | C | G | T | – | | – | C | C | T | , | A | T | G | , | T | A | C | , | A | C | C | , | C | A | T | , | C | C | C | , | A | G | T | , | G | C | A | – | | | | – | T | A | G | , | T | C | C | –3′ | | – | A | T | C | , | A | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTAGAA-3′ and 5′-GGACTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 25bd_8df5
| 5′– | A | A | T | , | G | A | C | – | | 3′– | T | T | A | , | C | T | G | – | | | | – | C | G | T | , | T | G | A | , | T | T | G | , | G | T | A | , | C | C | A | , | A | G | A | , | G | T | G | , | G | C | A | – | | – | G | C | A | , | A | C | T | , | A | A | C | , | C | A | T | , | G | G | T | , | T | C | T | , | C | A | C | , | C | G | T | – | | | | – | T | A | C | , | A | T | C | –3′ | | – | A | T | G | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AATGAC-3′ and 5′-GATGTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8347_9242
| 5′– | A | A | T | , | G | G | C | – | | 3′– | T | T | A | , | C | C | G | – | | | | – | C | G | A | , | G | C | T | , | G | A | G | , | C | T | T | , | C | C | G | , | T | T | T | , | C | T | G | , | C | G | A | – | | – | G | C | T | , | C | G | A | , | C | T | C | , | G | A | A | , | G | G | C | , | A | A | A | , | G | A | C | , | G | C | T | – | | | | – | C | A | A | , | T | G | A | –3′ | | – | G | T | T | , | A | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AATGGC-3′ and 5′-TCATTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7c95_4cf0
| 5′– | C | A | G | , | G | T | T | – | | 3′– | G | T | C | , | C | A | A | – | | | | – | A | C | G | , | T | T | G | , | T | G | C | , | G | A | G | , | A | G | A | , | T | A | G | , | C | A | A | , | G | T | G | – | | – | T | G | C | , | A | A | C | , | A | C | G | , | C | T | C | , | T | C | T | , | A | T | C | , | G | T | T | , | C | A | C | – | | | | – | G | A | T | , | C | A | A | –3′ | | – | C | T | A | , | G | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGGTT-3′ and 5′-TTGATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2971_0eaa
| 5′– | C | G | T | , | C | A | T | – | | 3′– | G | C | A | , | G | T | A | – | | | | – | A | T | C | , | G | T | G | , | A | T | A | , | G | A | C | , | G | C | T | , | C | T | T | , | G | A | T | , | A | C | G | – | | – | T | A | G | , | C | A | C | , | T | A | T | , | C | T | G | , | C | G | A | , | G | A | A | , | C | T | A | , | T | G | C | – | | | | – | C | G | T | , | G | G | A | –3′ | | – | G | C | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTCAT-3′ and 5′-TCCACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC ce1d_ff86
| 5′– | T | A | A | , | C | A | G | – | | 3′– | A | T | T | , | G | T | C | – | | | | – | T | G | G | , | G | A | C | , | T | T | A | , | G | G | G | , | A | G | A | , | G | A | A | , | G | T | A | , | C | G | G | – | | – | A | C | C | , | C | T | G | , | A | A | T | , | C | C | C | , | T | C | T | , | C | T | T | , | C | A | T | , | G | C | C | – | | | | – | T | C | C | , | A | A | G | –3′ | | – | A | G | G | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAACAG-3′ and 5′-CTTGGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 5da5_c2eb
| 5′– | A | G | G | , | T | A | G | – | | 3′– | T | C | C | , | A | T | C | – | | | | – | G | T | G | , | T | C | A | , | A | G | T | , | C | T | C | , | T | T | C | , | A | C | A | , | A | G | C | , | C | A | T | – | | – | C | A | C | , | A | G | T | , | T | C | A | , | G | A | G | , | A | A | G | , | T | G | T | , | T | C | G | , | G | T | A | – | | | | – | G | T | C | , | C | C | A | –3′ | | – | C | A | G | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTAG-3′ and 5′-TGGGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8b54_5ea4
| 5′– | T | G | A | , | A | T | C | – | | 3′– | A | C | T | , | T | A | G | – | | | | – | T | G | A | , | C | A | G | , | C | T | G | , | C | C | A | , | T | A | G | , | A | C | G | , | A | G | T | , | A | A | C | – | | – | A | C | T | , | G | T | C | , | G | A | C | , | G | G | T | , | A | T | C | , | T | G | C | , | T | C | A | , | T | T | G | – | | | | – | C | G | A | , | G | A | T | –3′ | | – | G | C | T | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAATC-3′ and 5′-ATCTCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC e15a_a64f
| 5′– | C | G | T | , | A | C | T | – | | 3′– | G | C | A | , | T | G | A | – | | | | – | G | T | C | , | A | G | A | , | C | G | T | , | A | T | C | , | T | G | C | , | T | A | C | , | T | G | T | , | C | G | A | – | | – | C | A | G | , | T | C | T | , | G | C | A | , | T | A | G | , | A | C | G | , | A | T | G | , | A | C | A | , | G | C | T | – | | | | – | G | C | T | , | G | C | A | –3′ | | – | C | G | A | , | C | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTACT-3′ and 5′-TGCAGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 79ef_dcef
| 5′– | G | T | C | , | C | T | A | – | | 3′– | C | A | G | , | G | A | T | – | | | | – | G | A | C | , | T | G | A | , | C | A | G | , | G | C | A | , | G | A | C | , | A | G | A | , | C | T | A | , | G | G | A | – | | – | C | T | G | , | A | C | T | , | G | T | C | , | C | G | T | , | C | T | G | , | T | C | T | , | G | A | T | , | C | C | T | – | | | | – | G | A | C | , | T | T | A | –3′ | | – | C | T | G | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCCTA-3′ and 5′-TAAGTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC cf85_fd4b
| 5′– | T | A | C | , | T | G | C | – | | 3′– | A | T | G | , | A | C | G | – | | | | – | A | G | A | , | A | T | C | , | A | T | C | , | G | G | T | , | C | G | C | , | T | G | G | , | G | T | T | , | C | A | C | – | | – | T | C | T | , | T | A | G | , | T | A | G | , | C | C | A | , | G | C | G | , | A | C | C | , | C | A | A | , | G | T | G | – | | | | – | A | T | C | , | A | G | G | –3′ | | – | T | A | G | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TACTGC-3′ and 5′-CCTGAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 75e4_ea47
| 5′– | C | A | A | , | A | G | T | – | | 3′– | G | T | T | , | T | C | A | – | | | | – | T | C | G | , | G | A | G | , | C | A | G | , | G | G | A | , | G | G | G | , | T | A | A | , | C | C | T | , | C | A | G | – | | – | A | G | C | , | C | T | C | , | G | T | C | , | C | C | T | , | C | C | C | , | A | T | T | , | G | G | A | , | G | T | C | – | | | | – | C | T | A | , | C | G | A | –3′ | | – | G | A | T | , | G | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAAAGT-3′ and 5′-TCGTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 44f8_716f
| 5′– | A | G | T | , | T | T | G | – | | 3′– | T | C | A | , | A | A | C | – | | | | – | T | A | G | , | C | A | C | , | T | G | T | , | A | C | G | , | T | C | A | , | A | G | C | , | T | A | G | , | T | G | C | – | | – | A | T | C | , | G | T | G | , | A | C | A | , | T | G | C | , | A | G | T | , | T | C | G | , | A | T | C | , | A | C | G | – | | | | – | G | G | T | , | G | A | T | –3′ | | – | C | C | A | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTTTG-3′ and 5′-ATCACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 2921_b11d
| 5′– | A | G | T | , | C | G | G | – | | 3′– | T | C | A | , | G | C | C | – | | | | – | A | A | G | , | T | T | C | , | G | C | T | , | C | C | C | , | T | A | G | , | G | C | A | , | A | G | C | , | T | G | G | – | | – | T | T | C | , | A | A | G | , | C | G | A | , | G | G | G | , | A | T | C | , | C | G | T | , | T | C | G | , | A | C | C | – | | | | – | G | G | C | , | A | A | T | –3′ | | – | C | C | G | , | T | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTCGG-3′ and 5′-ATTGCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC c7fa_87d7
| 5′– | C | C | A | , | C | G | T | – | | 3′– | G | G | T | , | G | C | A | – | | | | – | G | A | T | , | T | C | T | , | T | T | C | , | A | G | G | , | A | A | T | , | C | T | T | , | G | G | C | , | T | T | A | – | | – | C | T | A | , | A | G | A | , | A | A | G | , | T | C | C | , | T | T | A | , | G | A | A | , | C | C | G | , | A | A | T | – | | | | – | T | T | G | , | A | T | C | –3′ | | – | A | A | C | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCACGT-3′ and 5′-GATCAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 3840_a9b8
| 5′– | A | C | G | , | T | C | G | – | | 3′– | T | G | C | , | A | G | C | – | | | | – | C | T | G | , | A | G | A | , | C | C | A | , | A | G | T | , | T | C | G | , | T | T | C | , | G | C | T | , | A | C | A | – | | – | G | A | C | , | T | C | T | , | G | G | T | , | T | C | A | , | A | G | C | , | A | A | G | , | C | G | A | , | T | G | T | – | | | | – | C | A | T | , | G | A | A | –3′ | | – | G | T | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACGTCG-3′ and 5′-TTCATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC ea16_b305
| 5′– | A | T | G | , | T | C | C | – | | 3′– | T | A | C | , | A | G | G | – | | | | – | T | A | G | , | A | G | C | , | T | A | G | , | G | G | T | , | T | A | G | , | G | G | A | , | T | A | C | , | C | T | G | – | | – | A | T | C | , | T | C | G | , | A | T | C | , | C | C | A | , | A | T | C | , | C | C | T | , | A | T | G | , | G | A | C | – | | | | – | T | A | G | , | T | G | G | –3′ | | – | A | T | C | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGTCC-3′ and 5′-CCACTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c18c_fcb0
| 5′– | A | G | T | , | G | T | C | – | | 3′– | T | C | A | , | C | A | G | – | | | | – | C | C | T | , | A | A | G | , | T | G | C | , | A | G | A | , | C | A | T | , | C | A | C | , | G | A | C | , | A | G | T | – | | – | G | G | A | , | T | T | C | , | A | C | G | , | T | C | T | , | G | T | A | , | G | T | G | , | C | T | G | , | T | C | A | – | | | | – | T | C | A | , | T | G | G | –3′ | | – | A | G | T | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTGTC-3′ and 5′-CCATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 71fe_5d1d
| 5′– | T | G | C | , | T | A | C | – | | 3′– | A | C | G | , | A | T | G | – | | | | – | G | T | C | , | A | T | C | , | T | A | G | , | C | A | G | , | A | G | C | , | A | G | T | , | G | C | G | , | T | G | A | – | | – | C | A | G | , | T | A | G | , | A | T | C | , | G | T | C | , | T | C | G | , | T | C | A | , | C | G | C | , | A | C | T | – | | | | – | T | A | G | , | T | A | C | –3′ | | – | A | T | C | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCTAC-3′ and 5′-GTACTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC b53d_b4d9
| 5′– | A | G | T | , | A | C | C | – | | 3′– | T | C | A | , | T | G | G | – | | | | – | T | C | G | , | A | C | C | , | A | G | C | , | A | G | G | , | A | C | A | , | C | G | A | , | T | G | A | , | A | A | C | – | | – | A | G | C | , | T | G | G | , | T | C | G | , | T | C | C | , | T | G | T | , | G | C | T | , | A | C | T | , | T | T | G | – | | | | – | G | T | T | , | C | G | A | –3′ | | – | C | A | A | , | G | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTACC-3′ and 5′-TCGAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 87bd_f80f
| 5′– | T | C | C | , | G | A | C | – | | 3′– | A | G | G | , | C | T | G | – | | | | – | A | C | G | , | T | T | T | , | C | A | C | , | T | T | T | , | C | C | T | , | T | C | T | , | T | C | C | , | A | C | G | – | | – | T | G | C | , | A | A | A | , | G | T | G | , | A | A | A | , | G | G | A | , | A | G | A | , | A | G | G | , | T | G | C | – | | | | – | G | T | C | , | G | G | A | –3′ | | – | C | A | G | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCCGAC-3′ and 5′-TCCGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 2899_024c
| 5′– | T | A | A | , | G | C | C | – | | 3′– | A | T | T | , | C | G | G | – | | | | – | G | G | C | , | T | A | T | , | C | G | G | , | T | T | G | , | C | A | T | , | T | G | G | , | C | T | G | , | A | T | T | – | | – | C | C | G | , | A | T | A | , | G | C | C | , | A | A | C | , | G | T | A | , | A | C | C | , | G | A | C | , | T | A | A | – | | | | – | G | G | C | , | A | C | T | –3′ | | – | C | C | G | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAAGCC-3′ and 5′-AGTGCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC d29b_100f
| 5′– | A | T | T | , | G | A | C | – | | 3′– | T | A | A | , | C | T | G | – | | | | – | A | C | T | , | G | A | G | , | A | T | T | , | G | G | G | , | T | C | A | , | T | G | C | , | T | G | A | , | C | G | G | – | | – | T | G | A | , | C | T | C | , | T | A | A | , | C | C | C | , | A | G | T | , | A | C | G | , | A | C | T | , | G | C | C | – | | | | – | T | A | G | , | A | A | G | –3′ | | – | A | T | C | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATTGAC-3′ and 5′-CTTCTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC ec31_a3c8
| 5′– | G | G | C | , | A | A | T | – | | 3′– | C | C | G | , | T | T | A | – | | | | – | G | A | C | , | T | G | G | , | A | T | G | , | T | G | G | , | A | T | A | , | C | A | A | , | G | C | A | , | C | G | T | – | | – | C | T | G | , | A | C | C | , | T | A | C | , | A | C | C | , | T | A | T | , | G | T | T | , | C | G | T | , | G | C | A | – | | | | – | T | C | C | , | A | C | G | –3′ | | – | A | G | G | , | T | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGCAAT-3′ and 5′-CGTGGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 644d_cd74
| 5′– | T | G | G | , | A | A | G | – | | 3′– | A | C | C | , | T | T | C | – | | | | – | G | A | C | , | T | A | G | , | T | G | G | , | T | T | C | , | C | G | T | , | A | G | G | , | G | A | C | , | T | C | T | – | | – | C | T | G | , | A | T | C | , | A | C | C | , | A | A | G | , | G | C | A | , | T | C | C | , | C | T | G | , | A | G | A | – | | | | – | G | A | T | , | G | G | A | –3′ | | – | C | T | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGAAG-3′ and 5′-TCCATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 56a0_7410
| 5′– | T | C | A | , | C | A | G | – | | 3′– | A | G | T | , | G | T | C | – | | | | – | A | A | G | , | G | T | C | , | C | A | C | , | A | T | G | , | G | C | T | , | T | A | G | , | T | C | A | , | A | C | G | – | | – | T | T | C | , | C | A | G | , | G | T | G | , | T | A | C | , | C | G | A | , | A | T | C | , | A | G | T | , | T | G | C | – | | | | – | G | G | A | , | A | C | T | –3′ | | – | C | C | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCACAG-3′ and 5′-AGTTCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC e92a_78f2
| 5′– | A | G | T | , | A | G | C | – | | 3′– | T | C | A | , | T | C | G | – | | | | – | A | A | G | , | C | G | T | , | C | T | C | , | G | C | A | , | T | A | C | , | A | G | A | , | T | C | C | , | A | G | G | – | | – | T | T | C | , | G | C | A | , | G | A | G | , | C | G | T | , | A | T | G | , | T | C | T | , | A | G | G | , | T | C | C | – | | | | – | A | G | T | , | T | T | G | –3′ | | – | T | C | A | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTAGC-3′ and 5′-CAAACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 56fa_b457
| 5′– | T | A | G | , | A | C | C | – | | 3′– | A | T | C | , | T | G | G | – | | | | – | T | G | T | , | C | A | A | , | T | C | G | , | A | C | G | , | T | G | A | , | C | A | A | , | C | A | G | , | T | A | C | – | | – | A | C | A | , | G | T | T | , | A | G | C | , | T | G | C | , | A | C | T | , | G | T | T | , | G | T | C | , | A | T | G | – | | | | – | A | C | T | , | G | T | C | –3′ | | – | T | G | A | , | C | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGACC-3′ and 5′-GACAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 9ab6_4a56
| 5′– | G | C | A | , | G | T | A | – | | 3′– | C | G | T | , | C | A | T | – | | | | – | T | G | A | , | A | A | C | , | A | T | C | , | A | G | C | , | A | G | G | , | T | G | A | , | C | C | C | , | T | A | G | – | | – | A | C | T | , | T | T | G | , | T | A | G | , | T | C | G | , | T | C | C | , | A | C | T | , | G | G | G | , | A | T | C | – | | | | – | G | T | T | , | G | T | A | –3′ | | – | C | A | A | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCAGTA-3′ and 5′-TACAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 4886_f35f
| 5′– | A | T | G | , | A | G | G | – | | 3′– | T | A | C | , | T | C | C | – | | | | – | T | A | G | , | T | C | C | , | G | A | A | , | G | G | T | , | A | A | C | , | G | T | A | , | G | C | T | , | T | A | G | – | | – | A | T | C | , | A | G | G | , | C | T | T | , | C | C | A | , | T | T | G | , | C | A | T | , | C | G | A | , | A | T | C | – | | | | – | G | T | A | , | C | G | T | –3′ | | – | C | A | T | , | G | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGAGG-3′ and 5′-ACGTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c24f_77aa
| 5′– | C | T | G | , | T | G | A | – | | 3′– | G | A | C | , | A | C | T | – | | | | – | A | G | A | , | G | C | T | , | T | G | A | , | T | G | T | , | A | G | T | , | C | A | T | , | A | C | T | , | T | A | G | – | | – | T | C | T | , | C | G | A | , | A | C | T | , | A | C | A | , | T | C | A | , | G | T | A | , | T | G | A | , | A | T | C | – | | | | – | C | T | G | , | A | A | T | –3′ | | – | G | A | C | , | T | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTGTGA-3′ and 5′-ATTCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC e463_adca
| 5′– | A | T | C | , | T | C | G | – | | 3′– | T | A | G | , | A | G | C | – | | | | – | A | T | A | , | C | A | G | , | A | C | A | , | C | G | G | , | T | C | G | , | C | T | A | , | T | C | G | , | A | C | G | – | | – | T | A | T | , | G | T | C | , | T | G | T | , | G | C | C | , | A | G | C | , | G | A | T | , | A | G | C | , | T | G | C | – | | | | – | T | C | A | , | A | G | G | –3′ | | – | A | G | T | , | T | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCTCG-3′ and 5′-CCTTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC bdef_26a6
| 5′– | C | G | T | , | A | C | T | – | | 3′– | G | C | A | , | T | G | A | – | | | | – | C | C | G | , | A | G | T | , | C | C | A | , | C | A | G | , | G | T | T | , | C | A | A | , | G | G | T | , | T | C | A | – | | – | G | G | C | , | T | C | A | , | G | G | T | , | G | T | C | , | C | A | A | , | G | T | T | , | C | C | A | , | A | G | T | – | | | | – | T | A | G | , | A | A | G | –3′ | | – | A | T | C | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTACT-3′ and 5′-CTTCTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC a1f8_f4b8
| 5′– | G | T | A | , | G | T | T | – | | 3′– | C | A | T | , | C | A | A | – | | | | – | G | T | C | , | T | A | G | , | G | T | C | , | C | A | A | , | G | A | C | , | G | T | C | , | C | G | A | , | G | G | T | – | | – | C | A | G | , | A | T | C | , | C | A | G | , | G | T | T | , | C | T | G | , | C | A | G | , | G | C | T | , | C | C | A | – | | | | – | T | G | A | , | T | C | C | –3′ | | – | A | C | T | , | A | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTAGTT-3′ and 5′-GGATCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC b2b2_9125
| 5′– | C | G | A | , | T | C | A | – | | 3′– | G | C | T | , | A | G | T | – | | | | – | A | T | C | , | T | G | C | , | C | T | G | , | G | G | A | , | T | A | G | , | T | A | T | , | G | A | C | , | T | A | G | – | | – | T | A | G | , | A | C | G | , | G | A | C | , | C | C | T | , | A | T | C | , | A | T | A | , | C | T | G | , | A | T | C | – | | | | – | A | C | T | , | G | G | C | –3′ | | – | T | G | A | , | C | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGATCA-3′ and 5′-GCCAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 3a16_9c69
| 5′– | C | G | A | , | G | A | T | – | | 3′– | G | C | T | , | C | T | A | – | | | | – | T | A | T | , | C | A | G | , | G | T | G | , | T | A | G | , | G | A | C | , | T | A | G | , | G | C | T | , | A | T | G | – | | – | A | T | A | , | G | T | C | , | C | A | C | , | A | T | C | , | C | T | G | , | A | T | C | , | C | G | A | , | T | A | C | – | | | | – | C | T | G | , | G | A | T | –3′ | | – | G | A | C | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGAGAT-3′ and 5′-ATCCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC cfbb_e0d6
| 5′– | T | C | G | , | A | C | G | – | | 3′– | A | G | C | , | T | G | C | – | | | | – | T | A | C | , | G | T | C | , | T | C | T | , | A | C | G | , | C | T | T | , | G | T | C | , | C | C | T | , | T | A | G | – | | – | A | T | G | , | C | A | G | , | A | G | A | , | T | G | C | , | G | A | A | , | C | A | G | , | G | G | A | , | A | T | C | – | | | | – | A | C | C | , | T | T | G | –3′ | | – | T | G | G | , | A | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCGACG-3′ and 5′-CAAGGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC ad98_e9c2
| 5′– | T | T | G | , | A | T | G | – | | 3′– | A | A | C | , | T | A | C | – | | | | – | A | G | T | , | A | C | G | , | T | A | T | , | G | T | C | , | A | G | G | , | C | T | A | , | C | T | A | , | C | A | G | – | | – | T | C | A | , | T | G | C | , | A | T | A | , | C | A | G | , | T | C | C | , | G | A | T | , | G | A | T | , | G | T | C | – | | | | – | A | T | T | , | G | G | C | –3′ | | – | T | A | A | , | C | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTGATG-3′ and 5′-GCCAAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC fb29_a0ab
| 5′– | T | G | A | , | C | G | G | – | | 3′– | A | C | T | , | G | C | C | – | | | | – | G | A | C | , | C | A | T | , | A | G | A | , | C | G | A | , | G | C | C | , | A | G | T | , | C | C | G | , | T | C | A | – | | – | C | T | G | , | G | T | A | , | T | C | T | , | G | C | T | , | C | G | G | , | T | C | A | , | G | G | C | , | A | G | T | – | | | | – | C | T | G | , | C | C | A | –3′ | | – | G | A | C | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGACGG-3′ and 5′-TGGCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC ebf7_a221
| 5′– | T | A | G | , | T | T | C | – | | 3′– | A | T | C | , | A | A | G | – | | | | – | G | C | G | , | A | A | T | , | G | T | A | , | G | C | T | , | T | G | A | , | A | T | C | , | A | A | C | , | T | G | A | – | | – | C | G | C | , | T | T | A | , | C | A | T | , | C | G | A | , | A | C | T | , | T | A | G | , | T | T | G | , | A | C | T | – | | | | – | G | A | T | , | G | G | A | –3′ | | – | C | T | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAGTTC-3′ and 5′-TCCATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 6dd0_1451
| 5′– | A | C | T | , | G | A | C | – | | 3′– | T | G | A | , | C | T | G | – | | | | – | C | C | T | , | C | A | G | , | T | G | T | , | A | A | G | , | C | A | C | , | G | C | A | , | G | T | A | , | C | C | T | – | | – | G | G | A | , | G | T | C | , | A | C | A | , | T | T | C | , | G | T | G | , | C | G | T | , | C | A | T | , | G | G | A | – | | | | – | G | T | T | , | G | A | A | –3′ | | – | C | A | A | , | C | T | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACTGAC-3′ and 5′-TTCAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 194c_12b9
| 5′– | C | C | T | , | T | G | A | – | | 3′– | G | G | A | , | A | C | T | – | | | | – | G | C | C | , | A | T | C | , | A | C | A | , | T | C | A | , | A | G | A | , | T | C | A | , | C | A | A | , | T | G | A | – | | – | C | G | G | , | T | A | G | , | T | G | T | , | A | G | T | , | T | C | T | , | A | G | T | , | G | T | T | , | A | C | T | – | | | | – | C | T | T | , | G | A | T | –3′ | | – | G | A | A | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTTGA-3′ and 5′-ATCAAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 23f1_60cd
| 5′– | G | T | A | , | C | C | T | – | | 3′– | C | A | T | , | G | G | A | – | | | | – | A | C | T | , | G | A | G | , | C | T | G | , | T | G | G | , | T | G | T | , | C | A | G | , | G | T | T | , | C | A | G | – | | – | T | G | A | , | C | T | C | , | G | A | C | , | A | C | C | , | A | C | A | , | G | T | C | , | C | A | A | , | G | T | C | – | | | | – | C | A | T | , | C | C | T | –3′ | | – | G | T | A | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTACCT-3′ and 5′-AGGATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 4e48_5b82
| 5′– | C | T | A | , | T | G | A | – | | 3′– | G | A | T | , | A | C | T | – | | | | – | C | T | G | , | A | T | G | , | G | G | A | , | G | C | A | , | T | A | G | , | T | T | T | , | G | C | A | , | G | C | T | – | | – | G | A | C | , | T | A | C | , | C | C | T | , | C | G | T | , | A | T | C | , | A | A | A | , | C | G | T | , | C | G | A | – | | | | – | A | A | T | , | C | A | G | –3′ | | – | T | T | A | , | G | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTATGA-3′ and 5′-CTGATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 5423_8fb2
| 5′– | T | C | C | , | A | T | G | – | | 3′– | A | G | G | , | T | A | C | – | | | | – | C | T | A | , | C | G | A | , | C | A | C | , | A | C | A | , | G | T | G | , | G | T | G | , | C | T | A | , | G | T | A | – | | – | G | A | T | , | G | C | T | , | G | T | G | , | T | G | T | , | C | A | C | , | C | A | C | , | G | A | T | , | C | A | T | – | | | | – | T | G | A | , | T | C | G | –3′ | | – | A | C | T | , | A | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCCATG-3′ and 5′-CGATCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 45ac_2da1
| 5′– | C | G | T | , | C | C | A | – | | 3′– | G | C | A | , | G | G | T | – | | | | – | G | G | A | , | C | A | T | , | C | C | G | , | T | C | T | , | C | T | G | , | A | C | C | , | C | A | A | , | G | T | T | – | | – | C | C | T | , | G | T | A | , | G | G | C | , | A | G | A | , | G | A | C | , | T | G | G | , | G | T | T | , | C | A | A | – | | | | – | G | A | A | , | C | C | T | –3′ | | – | C | T | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTCCA-3′ and 5′-AGGTTC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 3e7c_453d
| 5′– | A | G | G | , | T | T | G | – | | 3′– | T | C | C | , | A | A | C | – | | | | – | G | C | C | , | T | A | T | , | C | T | A | , | G | T | G | , | G | T | G | , | G | G | A | , | A | G | C | , | A | T | T | – | | – | C | G | G | , | A | T | A | , | G | A | T | , | C | A | C | , | C | A | C | , | C | C | T | , | T | C | G | , | T | A | A | – | | | | – | A | T | G | , | C | T | G | –3′ | | – | T | A | C | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTTG-3′ and 5′-CAGCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8aed_9aae
| 5′– | A | C | G | , | T | T | C | – | | 3′– | T | G | C | , | A | A | G | – | | | | – | A | C | C | , | T | G | T | , | A | C | G | , | T | A | C | , | C | T | C | , | T | T | A | , | C | A | A | , | T | G | G | – | | – | T | G | G | , | A | C | A | , | T | G | C | , | A | T | G | , | G | A | G | , | A | A | T | , | G | T | T | , | A | C | C | – | | | | – | C | G | T | , | C | G | A | –3′ | | – | G | C | A | , | G | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACGTTC-3′ and 5′-TCGACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 98b6_457d
| 5′– | T | G | A | , | A | C | C | – | | 3′– | A | C | T | , | T | G | G | – | | | | – | G | C | A | , | A | T | G | , | G | G | T | , | T | C | A | , | C | T | T | , | T | G | C | , | G | T | C | , | A | C | A | – | | – | C | G | T | , | T | A | C | , | C | C | A | , | A | G | T | , | G | A | A | , | A | C | G | , | C | A | G | , | T | G | T | – | | | | – | T | G | A | , | G | C | C | –3′ | | – | A | C | T | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAACC-3′ and 5′-GGCTCA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 8281_b773
| 5′– | A | A | G | , | C | T | G | – | | 3′– | T | T | C | , | G | A | C | – | | | | – | A | T | G | , | T | C | C | , | T | G | T | , | G | C | C | , | A | A | C | , | C | T | C | , | A | G | C | , | T | A | C | – | | – | T | A | C | , | A | G | G | , | A | C | A | , | C | G | G | , | T | T | G | , | G | A | G | , | T | C | G | , | A | T | G | – | | | | – | C | A | G | , | A | C | T | –3′ | | – | G | T | C | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AAGCTG-3′ and 5′-AGTCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC dd8a_0297
| 5′– | A | T | G | , | A | G | G | – | | 3′– | T | A | C | , | T | C | C | – | | | | – | C | G | A | , | G | T | C | , | G | A | C | , | C | A | C | , | G | G | T | , | T | G | C | , | G | T | C | , | G | T | A | – | | – | G | C | T | , | C | A | G | , | C | T | G | , | G | T | G | , | C | C | A | , | A | C | G | , | C | A | G | , | C | A | T | – | | | | – | A | A | T | , | G | G | C | –3′ | | – | T | T | A | , | C | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGAGG-3′ and 5′-GCCATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 8801_5f84
| 5′– | T | G | A | , | G | T | C | – | | 3′– | A | C | T | , | C | A | G | – | | | | – | G | A | C | , | T | G | G | , | T | G | G | , | A | A | G | , | G | T | A | , | G | T | C | , | C | G | T | , | T | C | A | – | | – | C | T | G | , | A | C | C | , | A | C | C | , | T | T | C | , | C | A | T | , | C | A | G | , | G | C | A | , | A | G | T | – | | | | – | C | A | T | , | A | G | T | –3′ | | – | G | T | A | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAGTC-3′ and 5′-ACTATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 8ff4_47d1
| 5′– | T | G | A | , | G | G | C | – | | 3′– | A | C | T | , | C | C | G | – | | | | – | A | G | T | , | C | A | T | , | A | C | G | , | A | T | C | , | G | A | C | , | A | G | C | , | A | G | C | , | T | T | G | – | | – | T | C | A | , | G | T | A | , | T | G | C | , | T | A | G | , | C | T | G | , | T | C | G | , | T | C | G | , | A | A | C | – | | | | – | A | A | G | , | T | A | G | –3′ | | – | T | T | C | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGAGGC-3′ and 5′-CTACTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3a5d_8e6e
| 5′– | C | A | G | , | C | C | T | – | | 3′– | G | T | C | , | G | G | A | – | | | | – | A | G | A | , | C | A | T | , | T | G | A | , | T | C | G | , | A | T | A | , | C | A | A | , | T | G | C | , | A | C | G | – | | – | T | C | T | , | G | T | A | , | A | C | T | , | A | G | C | , | T | A | T | , | G | T | T | , | A | C | G | , | T | G | C | – | | | | – | C | C | A | , | C | T | A | –3′ | | – | G | G | T | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGCCT-3′ and 5′-TAGTGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 990b_35cc
| 5′– | C | A | G | , | T | C | A | – | | 3′– | G | T | C | , | A | G | T | – | | | | – | G | C | T | , | C | C | A | , | C | C | A | , | T | C | A | , | A | C | A | , | T | A | G | , | G | A | G | , | T | C | A | – | | – | C | G | A | , | G | G | T | , | G | G | T | , | A | G | T | , | T | G | T | , | A | T | C | , | C | T | C | , | A | G | T | – | | | | – | C | C | G | , | T | T | A | –3′ | | – | G | G | C | , | A | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGTCA-3′ and 5′-TAACGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 3a58_072d
| 5′– | A | C | G | , | G | T | C | – | | 3′– | T | G | C | , | C | A | G | – | | | | – | C | G | T | , | T | A | C | , | A | G | T | , | C | G | A | , | T | G | T | , | T | C | A | , | C | A | C | , | G | T | T | – | | – | G | C | A | , | A | T | G | , | T | C | A | , | G | C | T | , | A | C | A | , | A | G | T | , | G | T | G | , | C | A | A | – | | | | – | C | G | T | , | G | A | T | –3′ | | – | G | C | A | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACGGTC-3′ and 5′-ATCACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 4c9c_ffec
| 5′– | G | A | T | , | G | C | T | – | | 3′– | C | T | A | , | C | G | A | – | | | | – | C | C | G | , | T | C | A | , | T | A | C | , | T | A | C | , | A | A | T | , | C | A | G | , | G | T | C | , | C | T | A | – | | – | G | G | C | , | A | G | T | , | A | T | G | , | A | T | G | , | T | T | A | , | G | T | C | , | C | A | G | , | G | A | T | – | | | | – | A | T | T | , | G | G | C | –3′ | | – | T | A | A | , | C | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATGCT-3′ and 5′-GCCAAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 84d2_24ec
| 5′– | A | A | C | , | T | T | C | – | | 3′– | T | T | G | , | A | A | G | – | | | | – | G | A | C | , | C | T | T | , | A | C | G | , | A | T | T | , | C | C | G | , | A | G | T | , | T | C | T | , | G | G | A | – | | – | C | T | G | , | G | A | A | , | T | G | C | , | T | A | A | , | G | G | C | , | T | C | A | , | A | G | A | , | C | C | T | – | | | | – | G | G | C | , | T | C | A | –3′ | | – | C | C | G | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AACTTC-3′ and 5′-TGAGCC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 4123_9952
| 5′– | A | G | G | , | T | G | G | – | | 3′– | T | C | C | , | A | C | C | – | | | | – | A | G | C | , | C | A | T | , | T | C | A | , | C | T | C | , | A | A | C | , | C | T | C | , | A | A | G | , | T | G | C | – | | – | T | C | G | , | G | T | A | , | A | G | T | , | G | A | G | , | T | T | G | , | G | A | G | , | T | T | C | , | A | C | G | – | | | | – | G | T | C | , | A | A | T | –3′ | | – | C | A | G | , | T | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGGTGG-3′ and 5′-ATTGAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 9b8e_ec7b
| 5′– | C | T | A | , | C | C | T | – | | 3′– | G | A | T | , | G | G | A | – | | | | – | T | G | C | , | G | T | A | , | C | T | C | , | C | G | T | , | A | G | T | , | A | G | G | , | A | T | A | , | C | G | C | – | | – | A | C | G | , | C | A | T | , | G | A | G | , | G | C | A | , | T | C | A | , | T | C | C | , | T | A | T | , | G | C | G | – | | | | – | T | C | A | , | C | C | G | –3′ | | – | A | G | T | , | G | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTACCT-3′ and 5′-CGGTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC d07a_521d
| 5′– | C | C | T | , | A | G | T | – | | 3′– | G | G | A | , | T | C | A | – | | | | – | G | T | C | , | T | G | A | , | C | C | T | , | T | A | C | , | T | A | C | , | T | T | T | , | C | T | G | , | C | T | A | – | | – | C | A | G | , | A | C | T | , | G | G | A | , | A | T | G | , | A | T | G | , | A | A | A | , | G | A | C | , | G | A | T | – | | | | – | T | C | A | , | C | G | G | –3′ | | – | A | G | T | , | G | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTAGT-3′ and 5′-CCGTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 1809_af87
| 5′– | G | T | C | , | T | G | A | – | | 3′– | C | A | G | , | A | C | T | – | | | | – | T | C | G | , | A | G | T | , | C | A | G | , | A | A | G | , | T | A | T | , | G | G | G | , | T | C | A | , | A | T | G | – | | – | A | G | C | , | T | C | A | , | G | T | C | , | T | T | C | , | A | T | A | , | C | C | C | , | A | G | T | , | T | A | C | – | | | | – | C | C | T | , | C | C | A | –3′ | | – | G | G | A | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCTGA-3′ and 5′-TGGAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 7378_c60d
| 5′– | G | C | T | , | A | C | T | – | | 3′– | C | G | A | , | T | G | A | – | | | | – | T | G | T | , | C | C | A | , | A | C | A | , | T | C | G | , | A | G | C | , | T | T | A | , | G | C | A | , | T | T | C | – | | – | A | C | A | , | G | G | T | , | T | G | T | , | A | G | C | , | T | C | G | , | A | A | T | , | C | G | T | , | A | A | G | – | | | | – | T | A | C | , | G | A | C | –3′ | | – | A | T | G | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCTACT-3′ and 5′-GTCGTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 4005_0221
| 5′– | G | T | A | , | G | A | A | – | | 3′– | C | A | T | , | C | T | T | – | | | | – | T | A | T | , | C | T | G | , | C | C | T | , | C | A | A | , | G | T | A | , | T | G | G | , | T | G | G | , | C | A | G | – | | – | A | T | A | , | G | A | C | , | G | G | A | , | G | T | T | , | C | A | T | , | A | C | C | , | A | C | C | , | G | T | C | – | | | | – | G | C | A | , | C | A | T | –3′ | | – | C | G | T | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTAGAA-3′ and 5′-ATGTGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 1571_aff1
| 5′– | C | A | G | , | C | T | T | – | | 3′– | G | T | C | , | G | A | A | – | | | | – | A | T | G | , | C | T | G | , | G | A | T | , | G | G | G | , | T | A | C | , | A | G | G | , | A | A | C | , | T | G | G | – | | – | T | A | C | , | G | A | C | , | C | T | A | , | C | C | C | , | A | T | G | , | T | C | C | , | T | T | G | , | A | C | C | – | | | | – | T | A | C | , | A | C | G | –3′ | | – | A | T | G | , | T | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGCTT-3′ and 5′-CGTGTA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 1cc8_9ea8
| 5′– | A | A | T | , | G | G | C | – | | 3′– | T | T | A | , | C | C | G | – | | | | – | G | C | C | , | T | A | G | , | C | A | C | , | C | T | G | , | T | A | G | , | A | A | G | , | T | A | T | , | G | C | A | – | | – | C | G | G | , | A | T | C | , | G | T | G | , | G | A | C | , | A | T | C | , | T | T | C | , | A | T | A | , | C | G | T | – | | | | – | G | T | T | , | A | C | T | –3′ | | – | C | A | A | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AATGGC-3′ and 5′-AGTAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 7158_6b07
| 5′– | A | C | C | , | C | T | G | – | | 3′– | T | G | G | , | G | A | C | – | | | | – | G | A | T | , | C | C | T | , | T | A | C | , | A | C | G | , | A | T | G | , | G | T | C | , | A | G | C | , | T | T | T | – | | – | C | T | A | , | G | G | A | , | A | T | G | , | T | G | C | , | T | A | C | , | C | A | G | , | T | C | G | , | A | A | A | – | | | | – | A | T | G | , | A | T | C | –3′ | | – | T | A | C | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACCCTG-3′ and 5′-GATCAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC f4f3_6ae7
| 5′– | A | T | C | , | A | C | G | – | | 3′– | T | A | G | , | T | G | C | – | | | | – | A | C | G | , | A | C | T | , | G | T | G | , | T | G | A | , | G | T | T | , | G | T | A | , | C | A | T | , | C | T | G | – | | – | T | G | C | , | T | G | A | , | C | A | C | , | A | C | T | , | C | A | A | , | C | A | T | , | G | T | A | , | G | A | C | – | | | | – | C | G | T | , | G | G | A | –3′ | | – | G | C | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCACG-3′ and 5′-TCCACG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC d8e6_c38e
| 5′– | T | G | G | , | A | C | G | – | | 3′– | A | C | C | , | T | G | C | – | | | | – | T | G | C | , | A | T | T | , | G | G | T | , | T | C | T | , | G | A | A | , | A | G | T | , | T | A | C | , | T | A | G | – | | – | A | C | G | , | T | A | A | , | C | C | A | , | A | G | A | , | C | T | T | , | T | C | A | , | A | T | G | , | A | T | C | – | | | | – | T | C | A | , | T | A | G | –3′ | | – | A | G | T | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGGACG-3′ and 5′-CTATGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 3859_b5d0
| 5′– | A | T | C | , | T | T | C | – | | 3′– | T | A | G | , | A | A | G | – | | | | – | T | C | G | , | G | A | T | , | C | C | T | , | T | T | C | , | G | A | T | , | T | C | G | , | A | A | G | , | G | T | C | – | | – | A | G | C | , | C | T | A | , | G | G | A | , | A | A | G | , | C | T | A | , | A | G | C | , | T | T | C | , | C | A | G | – | | | | – | G | T | A | , | G | G | A | –3′ | | – | C | A | T | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATCTTC-3′ and 5′-TCCTAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 420e_a251
| 5′– | C | G | G | , | A | G | T | – | | 3′– | G | C | C | , | T | C | A | – | | | | – | C | T | G | , | C | A | T | , | G | G | T | , | C | A | C | , | C | A | G | , | A | C | G | , | T | G | T | , | C | G | A | – | | – | G | A | C | , | G | T | A | , | C | C | A | , | G | T | G | , | G | T | C | , | T | G | C | , | A | C | A | , | G | C | T | – | | | | – | G | G | T | , | C | T | A | –3′ | | – | C | C | A | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGGAGT-3′ and 5′-TAGACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC ed98_900a
| 5′– | G | C | C | , | A | G | T | – | | 3′– | C | G | G | , | T | C | A | – | | | | – | C | T | G | , | A | C | G | , | T | T | A | , | C | C | C | , | T | A | C | , | A | G | G | , | G | A | A | , | G | C | T | – | | – | G | A | C | , | T | G | C | , | A | A | T | , | G | G | G | , | A | T | G | , | T | C | C | , | C | T | T | , | C | G | A | – | | | | – | C | A | T | , | T | C | A | –3′ | | – | G | T | A | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCCAGT-3′ and 5′-TGAATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC dbb1_8911
| 5′– | T | G | C | , | G | A | C | – | | 3′– | A | C | G | , | C | T | G | – | | | | – | T | G | T | , | C | T | A | , | A | G | T | , | C | T | T | , | T | G | C | , | C | A | A | , | T | G | T | , | T | A | C | – | | – | A | C | A | , | G | A | T | , | T | C | A | , | G | A | A | , | A | C | G | , | G | T | T | , | A | C | A | , | A | T | G | – | | | | – | A | G | G | , | T | G | C | –3′ | | – | T | C | C | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCGAC-3′ and 5′-GCACCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 2aad_cb69
| 5′– | C | A | G | , | C | T | T | – | | 3′– | G | T | C | , | G | A | A | – | | | | – | C | G | A | , | C | G | T | , | T | G | C | , | T | A | G | , | T | C | G | , | A | C | C | , | G | A | T | , | G | C | T | – | | – | G | C | T | , | G | C | A | , | A | C | G | , | A | T | C | , | A | G | C | , | T | G | G | , | C | T | A | , | C | G | A | – | | | | – | C | T | G | , | G | G | A | –3′ | | – | G | A | C | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGCTT-3′ and 5′-TCCCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 95e9_d6c6
| 5′– | C | C | T | , | C | A | T | – | | 3′– | G | G | A | , | G | T | A | – | | | | – | C | C | A | , | G | T | T | , | G | C | G | , | T | T | A | , | C | A | T | , | G | A | G | , | A | C | C | , | T | G | A | – | | – | G | G | T | , | C | A | A | , | C | G | C | , | A | A | T | , | G | T | A | , | C | T | C | , | T | G | G | , | A | C | T | – | | | | – | C | T | G | , | A | T | T | –3′ | | – | G | A | C | , | T | A | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCTCAT-3′ and 5′-AATCAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 75b9_2a5a
| 5′– | C | A | G | , | A | G | T | – | | 3′– | G | T | C | , | T | C | A | – | | | | – | G | A | C | , | G | G | T | , | T | A | C | , | T | G | T | , | A | G | A | , | C | T | A | , | C | C | A | , | T | G | A | – | | – | C | T | G | , | C | C | A | , | A | T | G | , | A | C | A | , | T | C | T | , | G | A | T | , | G | G | T | , | A | C | T | – | | | | – | C | C | T | , | G | T | A | –3′ | | – | G | G | A | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGAGT-3′ and 5′-TACAGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC dc0f_56c8
| 5′– | C | G | G | , | A | T | T | – | | 3′– | G | C | C | , | T | A | A | – | | | | – | G | A | T | , | C | G | A | , | C | T | C | , | T | T | G | , | G | C | A | , | T | A | G | , | A | G | C | , | T | C | A | – | | – | C | T | A | , | G | C | T | , | G | A | G | , | A | A | C | , | C | G | T | , | A | T | C | , | T | C | G | , | A | G | T | – | | | | – | G | A | T | , | G | C | A | –3′ | | – | C | T | A | , | C | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGGATT-3′ and 5′-TGCATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC fee8_9156
| 5′– | G | T | C | , | T | T | A | – | | 3′– | C | A | G | , | A | A | T | – | | | | – | T | C | A | , | G | T | T | , | G | A | G | , | G | A | T | , | C | T | T | , | C | G | A | , | G | G | A | , | C | T | C | – | | – | A | G | T | , | C | A | A | , | C | T | C | , | C | T | A | , | G | A | A | , | G | C | T | , | C | C | T | , | G | A | G | – | | | | – | A | G | C | , | T | G | G | –3′ | | – | T | C | G | , | A | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCTTA-3′ and 5′-CCAGCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 539b_9fc8
| 5′– | T | G | C | , | A | A | C | – | | 3′– | A | C | G | , | T | T | G | – | | | | – | T | T | A | , | G | C | T | , | C | T | T | , | C | T | G | , | T | A | C | , | A | G | T | , | T | G | G | , | C | A | G | – | | – | A | A | T | , | C | G | A | , | G | A | A | , | G | A | C | , | A | T | G | , | T | C | A | , | A | C | C | , | G | T | C | – | | | | – | G | T | T | , | G | G | A | –3′ | | – | C | A | A | , | C | C | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGCAAC-3′ and 5′-TCCAAC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 8905_3852
| 5′– | C | A | T | , | C | G | A | – | | 3′– | G | T | A | , | G | C | T | – | | | | – | C | G | A | , | T | T | G | , | G | C | T | , | C | G | A | , | G | C | C | , | A | A | T | , | C | C | A | , | A | G | T | – | | – | G | C | T | , | A | A | C | , | C | G | A | , | G | C | T | , | C | G | G | , | T | T | A | , | G | G | T | , | T | C | A | – | | | | – | A | C | T | , | A | C | G | –3′ | | – | T | G | A | , | T | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATCGA-3′ and 5′-CGTAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC cf2d_a83a
| 5′– | G | G | T | , | G | C | A | – | | 3′– | C | C | A | , | C | G | T | – | | | | – | G | C | A | , | G | T | C | , | C | C | T | , | C | T | T | , | C | T | A | , | G | C | T | , | C | T | G | , | A | A | A | – | | – | C | G | T | , | C | A | G | , | G | G | A | , | G | A | A | , | G | A | T | , | C | G | A | , | G | A | C | , | T | T | T | – | | | | – | A | A | C | , | T | C | G | –3′ | | – | T | T | G | , | A | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGTGCA-3′ and 5′-CGAGTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 0a64_e9f2
| 5′– | C | A | G | , | G | A | T | – | | 3′– | G | T | C | , | C | T | A | – | | | | – | A | T | G | , | C | T | G | , | T | G | A | , | C | A | C | , | A | G | T | , | A | G | C | , | C | T | C | , | A | C | G | – | | – | T | A | C | , | G | A | C | , | A | C | T | , | G | T | G | , | T | C | A | , | T | C | G | , | G | A | G | , | T | G | C | – | | | | – | T | C | G | , | A | A | G | –3′ | | – | A | G | C | , | T | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CAGGAT-3′ and 5′-CTTCGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 5ca8_0496
| 5′– | G | T | T | , | A | G | T | – | | 3′– | C | A | A | , | T | C | A | – | | | | – | A | T | C | , | A | A | G | , | A | G | T | , | C | A | C | , | A | C | A | , | C | T | C | , | A | T | A | , | C | G | C | – | | – | T | A | G | , | T | T | C | , | T | C | A | , | G | T | G | , | T | G | T | , | G | A | G | , | T | A | T | , | G | C | G | – | | | | – | A | A | G | , | C | T | G | –3′ | | – | T | T | C | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTAGT-3′ and 5′-CAGCTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 394c_a0b8
| 5′– | T | T | C | , | C | A | G | – | | 3′– | A | A | G | , | G | T | C | – | | | | – | A | G | C | , | T | C | A | , | T | G | G | , | A | G | T | , | T | G | T | , | C | T | A | , | G | C | T | , | C | A | G | – | | – | T | C | G | , | A | G | T | , | A | C | C | , | T | C | A | , | A | C | A | , | G | A | T | , | C | G | A | , | G | T | C | – | | | | – | T | C | G | , | A | C | G | –3′ | | – | A | G | C | , | T | G | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTCCAG-3′ and 5′-CGTCGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC b5f7_7a65
| 5′– | G | T | C | , | C | C | A | – | | 3′– | C | A | G | , | G | G | T | – | | | | – | A | C | T | , | A | G | G | , | T | C | A | , | C | G | A | , | C | A | G | , | G | T | C | , | A | T | A | , | C | G | C | – | | – | T | G | A | , | T | C | C | , | A | G | T | , | G | C | T | , | G | T | C | , | C | A | G | , | T | A | T | , | G | C | G | – | | | | – | A | G | G | , | T | T | C | –3′ | | – | T | C | C | , | A | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCCCA-3′ and 5′-GAACCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC e3ea_ae44
| 5′– | T | A | A | , | G | A | C | – | | 3′– | A | T | T | , | C | T | G | – | | | | – | T | C | A | , | A | A | G | , | G | A | A | , | T | C | C | , | A | A | C | , | A | G | C | , | G | T | A | , | A | C | G | – | | – | A | G | T | , | T | T | C | , | C | T | T | , | A | G | G | , | T | T | G | , | T | C | G | , | C | A | T | , | T | G | C | – | | | | – | C | A | G | , | C | T | A | –3′ | | – | G | T | C | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAAGAC-3′ and 5′-TAGCTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC c394_4a7b
| 5′– | T | T | A | , | C | G | G | – | | 3′– | A | A | T | , | G | C | C | – | | | | – | C | A | C | , | T | G | A | , | G | G | T | , | A | T | C | , | A | C | T | , | C | A | A | , | C | A | C | , | G | T | A | – | | – | G | T | G | , | A | C | T | , | C | C | A | , | T | A | G | , | T | G | A | , | G | T | T | , | G | T | G | , | C | A | T | – | | | | – | C | A | T | , | T | C | A | –3′ | | – | G | T | A | , | A | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTACGG-3′ and 5′-TGAATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC f48b_4f8e
| 5′– | T | T | G | , | A | G | C | – | | 3′– | A | A | C | , | T | C | G | – | | | | – | T | C | G | , | T | A | C | , | T | G | G | , | A | C | T | , | A | C | G | , | T | G | A | , | C | T | G | , | A | C | G | – | | – | A | G | C | , | A | T | G | , | A | C | C | , | T | G | A | , | T | G | C | , | A | C | T | , | G | A | C | , | T | G | C | – | | | | – | A | G | G | , | T | G | C | –3′ | | – | T | C | C | , | A | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TTGAGC-3′ and 5′-GCACCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 9b1a_52e5
| 5′– | C | T | A | , | C | T | T | – | | 3′– | G | A | T | , | G | A | A | – | | | | – | T | A | G | , | A | A | C | , | A | A | A | , | G | A | A | , | A | C | C | , | T | G | T | , | T | A | C | , | A | G | C | – | | – | A | T | C | , | T | T | G | , | T | T | T | , | C | T | T | , | T | G | G | , | A | C | A | , | A | T | G | , | T | C | G | – | | | | – | A | C | T | , | G | C | C | –3′ | | – | T | G | A | , | C | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTACTT-3′ and 5′-GGCAGT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 454e_99fb
| 5′– | G | T | A | , | T | C | A | – | | 3′– | C | A | T | , | A | G | T | – | | | | – | A | A | T | , | C | T | G | , | A | T | T | , | C | G | A | , | T | A | G | , | T | G | G | , | A | A | C | , | T | A | G | – | | – | T | T | A | , | G | A | C | , | T | A | A | , | G | C | T | , | A | T | C | , | A | C | C | , | T | T | G | , | A | T | C | – | | | | – | A | A | T | , | G | A | C | –3′ | | – | T | T | A | , | C | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTATCA-3′ and 5′-GTCATT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0314_2401
| 5′– | C | G | T | , | G | A | T | – | | 3′– | G | C | A | , | C | T | A | – | | | | – | G | C | G | , | A | T | C | , | G | C | T | , | A | T | G | , | C | T | A | , | T | C | A | , | C | C | G | , | T | G | A | – | | – | C | G | C | , | T | A | G | , | C | G | A | , | T | A | C | , | G | A | T | , | A | G | T | , | G | G | C | , | A | C | T | – | | | | – | C | C | G | , | A | C | T | –3′ | | – | G | G | C | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CGTGAT-3′ and 5′-AGTCGG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 4c76_9db1
| 5′– | G | T | T | , | G | G | A | – | | 3′– | C | A | A | , | C | C | T | – | | | | – | T | G | C | , | A | A | C | , | C | A | C | , | A | G | C | , | C | A | C | , | A | C | G | , | A | G | C | , | C | T | C | – | | – | A | C | G | , | T | T | G | , | G | T | G | , | T | C | G | , | G | T | G | , | T | G | C | , | T | C | G | , | G | A | G | – | | | | – | A | T | T | , | C | G | G | –3′ | | – | T | A | A | , | G | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTTGGA-3′ and 5′-CCGAAT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC a30a_9fa8
| 5′– | C | A | T | , | G | C | T | – | | 3′– | G | T | A | , | C | G | A | – | | | | – | C | A | A | , | G | C | T | , | C | T | A | , | C | T | G | , | G | C | A | , | C | A | C | , | T | C | A | , | G | T | A | – | | – | G | T | T | , | C | G | A | , | G | A | T | , | G | A | C | , | C | G | T | , | G | T | G | , | A | G | T | , | C | A | T | – | | | | – | T | C | C | , | A | G | C | –3′ | | – | A | G | G | , | T | C | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATGCT-3′ and 5′-GCTGGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 1560_3e84
| 5′– | C | T | T | , | A | C | T | – | | 3′– | G | A | A | , | T | G | A | – | | | | – | C | T | T | , | G | A | A | , | C | T | T | , | T | G | C | , | T | T | G | , | G | T | T | , | C | G | G | , | T | G | A | – | | – | G | A | A | , | C | T | T | , | G | A | A | , | A | C | G | , | A | A | C | , | C | A | A | , | G | C | C | , | A | C | T | – | | | | – | T | T | G | , | T | A | G | –3′ | | – | A | A | C | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CTTACT-3′ and 5′-CTACAA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC e12c_664a
| 5′– | T | A | A | , | C | A | G | – | | 3′– | A | T | T | , | G | T | C | – | | | | – | G | C | C | , | T | A | G | , | A | G | T | , | C | C | A | , | T | T | C | , | A | A | C | , | T | C | A | , | G | T | T | – | | – | C | G | G | , | A | T | C | , | T | C | A | , | G | G | T | , | A | A | G | , | T | T | G | , | A | G | T | , | C | A | A | – | | | | – | C | A | A | , | C | A | T | –3′ | | – | G | T | T | , | G | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TAACAG-3′ and 5′-ATGTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 1b04_c762
| 5′– | G | A | C | , | A | G | T | – | | 3′– | C | T | G | , | T | C | A | – | | | | – | A | C | G | , | T | C | A | , | C | A | G | , | T | C | C | , | G | T | G | , | T | T | G | , | C | A | T | , | G | G | G | – | | – | T | G | C | , | A | G | T | , | G | T | C | , | A | G | G | , | C | A | C | , | A | A | C | , | G | T | A | , | C | C | C | – | | | | – | T | C | A | , | C | T | G | –3′ | | – | A | G | T | , | G | A | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACAGT-3′ and 5′-CAGTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC bb94_c747
| 5′– | G | C | A | , | C | C | T | – | | 3′– | C | G | T | , | G | G | A | – | | | | – | T | A | G | , | T | C | G | , | T | T | G | , | C | C | A | , | C | T | G | , | T | C | T | , | A | G | C | , | T | A | G | – | | – | A | T | C | , | A | G | C | , | A | A | C | , | G | G | T | , | G | A | C | , | A | G | A | , | T | C | G | , | A | T | C | – | | | | – | C | G | A | , | G | G | T | –3′ | | – | G | C | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GCACCT-3′ and 5′-ACCTCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 6a2c_33e2
| 5′– | G | T | C | , | T | T | A | – | | 3′– | C | A | G | , | A | A | T | – | | | | – | C | T | G | , | A | T | C | , | G | A | C | , | G | A | G | , | T | C | T | , | C | A | C | , | A | A | G | , | G | C | T | – | | – | G | A | C | , | T | A | G | , | C | T | G | , | C | T | C | , | A | G | A | , | G | T | G | , | T | T | C | , | C | G | A | – | | | | – | A | A | G | , | T | A | C | –3′ | | – | T | T | C | , | A | T | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GTCTTA-3′ and 5′-GTACTT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 3c4d_9044
| 5′– | T | G | A | , | C | C | G | – | | 3′– | A | C | T | , | G | G | C | – | | | | – | G | C | C | , | A | T | C | , | G | T | A | , | A | C | T | , | T | T | C | , | A | G | A | , | A | G | C | , | C | T | A | – | | – | C | G | G | , | T | A | G | , | C | A | T | , | T | G | A | , | A | A | G | , | T | C | T | , | T | C | G | , | G | A | T | – | | | | – | T | C | G | , | A | C | C | –3′ | | – | A | G | C | , | T | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TGACCG-3′ and 5′-GGTCGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC afa1_aa8c
| 5′– | C | A | T | , | G | C | T | – | | 3′– | G | T | A | , | C | G | A | – | | | | – | A | C | G | , | T | A | T | , | G | T | G | , | A | A | T | , | C | G | T | , | G | T | T | , | C | A | T | , | T | G | G | – | | – | T | G | C | , | A | T | A | , | C | A | C | , | T | T | A | , | G | C | A | , | C | A | A | , | G | T | A | , | A | C | C | – | | | | – | C | A | T | , | C | C | A | –3′ | | – | G | T | A | , | G | G | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CATGCT-3′ and 5′-TGGATG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0cca_1ccc
| 5′– | A | G | T | , | T | C | G | – | | 3′– | T | C | A | , | A | G | C | – | | | | – | T | A | C | , | A | A | G | , | A | G | T | , | G | C | A | , | T | T | C | , | A | T | C | , | C | G | A | , | A | T | G | – | | – | A | T | G | , | T | T | C | , | T | C | A | , | C | G | T | , | A | A | G | , | T | A | G | , | G | C | T | , | T | A | C | – | | | | – | A | G | T | , | C | G | G | –3′ | | – | T | C | A | , | G | C | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AGTTCG-3′ and 5′-CCGACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC d78c_3f82
| 5′– | G | A | T | , | G | T | A | – | | 3′– | C | T | A | , | C | A | T | – | | | | – | T | G | C | , | C | A | A | , | A | G | A | , | G | A | T | , | T | G | C | , | A | A | C | , | T | A | A | , | C | G | C | – | | – | A | C | G | , | G | T | T | , | T | C | T | , | C | T | A | , | A | C | G | , | T | T | G | , | A | T | T | , | G | C | G | – | | | | – | G | G | T | , | C | T | A | –3′ | | – | C | C | A | , | G | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GATGTA-3′ and 5′-TAGACC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect
MC 0e0e_ba93
| 5′– | G | A | C | , | C | T | A | – | | 3′– | C | T | G | , | G | A | T | – | | | | – | G | C | A | , | G | G | T | , | A | A | C | , | T | A | C | , | T | G | G | , | C | T | G | , | A | C | G | , | T | G | T | – | | – | C | G | T | , | C | C | A | , | T | T | G | , | A | T | G | , | A | C | C | , | G | A | C | , | T | G | C | , | A | C | A | – | | | | – | G | C | A | , | A | C | T | –3′ | | – | C | G | T | , | T | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACCTA-3′ and 5′-AGTTGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 202d_a80e
| 5′– | C | C | G | , | A | A | T | – | | 3′– | G | G | C | , | T | T | A | – | | | | – | C | T | G | , | A | C | G | , | C | A | T | , | C | C | T | , | G | T | G | , | C | T | T | , | G | C | A | , | C | T | T | – | | – | G | A | C | , | T | G | C | , | G | T | A | , | G | G | A | , | C | A | C | , | G | A | A | , | C | G | T | , | G | A | A | – | | | | – | G | A | T | , | G | T | A | –3′ | | – | C | T | A | , | C | A | T | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-CCGAAT-3′ and 5′-TACATC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 4224_f2ce
| 5′– | A | A | T | , | C | A | G | – | | 3′– | T | T | A | , | G | T | C | – | | | | – | C | A | T | , | T | C | G | , | G | T | C | , | A | T | C | , | T | T | C | , | T | A | T | , | C | T | G | , | A | T | T | – | | – | G | T | A | , | A | G | C | , | C | A | G | , | T | A | G | , | A | A | G | , | A | T | A | , | G | A | C | , | T | A | A | – | | | | – | C | T | A | , | G | G | T | –3′ | | – | G | A | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-AATCAG-3′ and 5′-ACCTAG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect
MC 91d8_5890
| 5′– | A | C | G | , | T | C | G | – | | 3′– | T | G | C | , | A | G | C | – | | | | – | T | G | C | , | G | A | A | , | A | C | A | , | C | A | G | , | A | T | C | , | A | G | C | , | A | C | G | , | T | T | G | – | | – | A | C | G | , | C | T | T | , | T | G | T | , | G | T | C | , | T | A | G | , | T | C | G | , | T | G | C | , | A | A | C | – | | | | – | G | C | T | , | G | A | T | –3′ | | – | C | G | A | , | C | T | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACGTCG-3′ and 5′-ATCAGC-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 520d_5d41
| 5′– | T | C | A | , | A | C | G | – | | 3′– | A | G | T | , | T | G | C | – | | | | – | T | G | A | , | C | G | G | , | A | T | G | , | A | T | T | , | G | C | A | , | C | A | A | , | G | T | A | , | C | G | G | – | | – | A | C | T | , | G | C | C | , | T | A | C | , | T | A | A | , | C | G | T | , | G | T | T | , | C | A | T | , | G | C | C | – | | | | – | T | C | A | , | A | T | C | –3′ | | – | A | G | T | , | T | A | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCAACG-3′ and 5′-GATTGA-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 4310_7272
| 5′– | A | C | G | , | G | T | C | – | | 3′– | T | G | C | , | C | A | G | – | | | | – | G | G | C | , | A | T | T | , | G | T | A | , | C | C | A | , | A | T | C | , | T | C | C | , | G | T | C | , | T | T | A | – | | – | C | C | G | , | T | A | A | , | C | A | T | , | G | G | T | , | T | A | G | , | A | G | G | , | C | A | G | , | A | A | T | – | | | | – | A | G | C | , | T | C | C | –3′ | | – | T | C | G | , | A | G | G | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ACGGTC-3′ and 5′-GGAGCT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct
MC 9f3d_c4b8
| 5′– | G | A | C | , | G | T | A | – | | 3′– | C | T | G | , | C | A | T | – | | | | – | A | T | C | , | G | C | A | , | T | T | C | , | A | T | T | , | G | A | C | , | G | A | G | , | A | G | C | , | A | T | C | – | | – | T | A | G | , | C | G | T | , | A | A | G | , | T | A | A | , | C | T | G | , | C | T | C | , | T | C | G | , | T | A | G | – | | | | – | C | G | A | , | G | G | T | –3′ | | – | G | C | T | , | C | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GACGTA-3′ and 5′-ACCTCG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC 3320_32cc
| 5′– | G | G | A | , | T | G | A | – | | 3′– | C | C | T | , | A | C | T | – | | | | – | A | C | A | , | G | T | C | , | C | C | A | , | G | C | A | , | T | A | G | , | T | T | G | , | T | C | A | , | G | C | G | – | | – | T | G | T | , | C | A | G | , | G | G | T | , | C | G | T | , | A | T | C | , | A | A | C | , | A | G | T | , | C | G | C | – | | | | – | A | G | T | , | T | A | G | –3′ | | – | T | C | A | , | A | T | C | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-GGATGA-3′ and 5′-CTAACT-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect AND Incorrect
MC 4661_a7d8
| 5′– | T | C | G | , | A | A | G | – | | 3′– | A | G | C | , | T | T | C | – | | | | – | C | A | G | , | T | G | C | , | T | C | T | , | G | G | G | , | A | C | T | , | C | G | T | , | G | A | G | , | T | C | T | – | | – | G | T | C | , | A | C | G | , | A | G | A | , | C | C | C | , | T | G | A | , | G | C | A | , | C | T | C | , | A | G | A | – | | | | – | C | A | A | , | C | C | T | –3′ | | – | G | T | T | , | G | G | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-TCGAAG-3′ and 5′-AGGTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Correct AND Incorrect
MC b304_8a2d
| 5′– | A | T | G | , | G | A | C | – | | 3′– | T | A | C | , | C | T | G | – | | | | – | G | G | T | , | A | C | A | , | G | A | C | , | G | G | A | , | G | C | A | , | C | A | G | , | T | C | G | , | A | T | T | – | | – | C | C | A | , | T | G | T | , | C | T | G | , | C | C | T | , | C | G | T | , | G | T | C | , | A | G | C | , | T | A | A | – | | | | – | C | A | A | , | A | G | T | –3′ | | – | G | T | T | , | T | C | A | –5′ | |
The amplicon sequence of DNA shown above was replicated using 30 cycles of PCR, using the primers 5′-ATGGAC-3′ and 5′-ACTTTG-3′.
But the first PCR run contained significant contamination due to mispriming. Probably from using too short of primers that were only 6 nucleotide in length.
Choose the correct pair of RNA primers that will amplify the remaining region of DNA inside the old primers using nested PCR. The nested RNA primers are 6 bases in length.
Pay close attention to the 5′ and 3′ ends of the primers.
AND Correct AND Incorrect AND Incorrect AND Incorrect AND Incorrect AND Incorrect