## DESCRIPTION
## Select the DNA replication enzyme that corresponds to the given function.
## ENDDESCRIPTION
## KEYWORDS('DNA replication enzymes','functions','which one','multiple choice')
## DBsubject('Molecular Biology')
## DBchapter('')
## DBsection('')
## Date('2026-07-12')
## Author('Neil R. Voss')
## Institution('Roosevelt University')
DOCUMENT();
loadMacros(
'PGstandard.pl',
'PGML.pl',
'PGchoicemacros.pl',
'parserRadioButtons.pl',
'PGcourse.pl',
);
TEXT(beginproblem());
$showPartialCorrectAnswers = 0;
#==========================================================
# QUESTION DATA
#==========================================================
# All questions data
@questions_data = (
{
'item_name' => 'this enzyme relieves strain in the parental DNA double helix caused by the supercoiling',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA topoisomerase',
'DNA helicase',
'DNA ligase',
'DNA sliding clamp',
],
'correct' => 'DNA topoisomerase',
},
{
'item_name' => 'this enzyme releases the supercoiling tension in the DNA double helix',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA topoisomerase',
'DNA ligase',
'DNA polymerase Ⅲ',
'DNA sliding clamp',
],
'correct' => 'DNA topoisomerase',
},
{
'item_name' => 'this enzyme builds RNA primers using the parental DNA strand as a template',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'primase',
'DNA ligase',
'single-strand binding proteins',
'DNA sliding clamp',
],
'correct' => 'primase',
},
{
'item_name' => 'this enzyme creates the RNA primers that serve as a starting point for DNA synthesis',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'primase',
'DNA helicase',
'DNA polymerase Ⅰ',
'DNA sliding clamp',
],
'correct' => 'primase',
},
{
'item_name' => 'this enzyme unwinds the parental DNA double helix',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA topoisomerase',
'DNA helicase',
'DNA ligase',
'single-strand binding proteins',
],
'correct' => 'DNA helicase',
},
{
'item_name' => 'this enzyme separates the two strands of the DNA double helix',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA topoisomerase',
'DNA helicase',
'DNA polymerase Ⅰ',
'DNA sliding clamp',
],
'correct' => 'DNA helicase',
},
{
'item_name' => 'this enzyme joins Okazaki fragments together to create one continuous DNA strand',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA topoisomerase',
'DNA ligase',
'DNA polymerase Ⅰ',
'DNA polymerase Ⅲ',
],
'correct' => 'DNA ligase',
},
{
'item_name' => 'this enzyme seals the gaps between the Okazaki fragments to create one continuous DNA strand',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA topoisomerase',
'DNA ligase',
'DNA polymerase Ⅲ',
'DNA sliding clamp',
],
'correct' => 'DNA ligase',
},
{
'item_name' => 'this protein holds the DNA strands apart while they are being replicated',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA helicase',
'single-strand binding proteins',
'DNA polymerase Ⅰ',
'DNA polymerase Ⅲ',
],
'correct' => 'single-strand binding proteins',
},
{
'item_name' => 'this protein stabilizes the open conformation of the unwound DNA',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'primase',
'DNA helicase',
'single-strand binding proteins',
'DNA sliding clamp',
],
'correct' => 'single-strand binding proteins',
},
{
'item_name' => 'this enzyme replaces the RNA primers with DNA in the Okazaki fragments',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA ligase',
'DNA polymerase Ⅰ',
'DNA polymerase Ⅲ',
'DNA sliding clamp',
],
'correct' => 'DNA polymerase Ⅰ',
},
{
'item_name' => 'this enzyme removes RNA primers and fills the gaps with DNA between Okazaki fragments',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'primase',
'DNA ligase',
'DNA polymerase Ⅰ',
'DNA polymerase Ⅲ',
],
'correct' => 'DNA polymerase Ⅰ',
},
{
'item_name' => 'this enzyme removes the RNA primers at the beginning of each Okazaki fragment and replaces them with DNA',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'primase',
'DNA helicase',
'single-strand binding proteins',
'DNA polymerase Ⅰ',
],
'correct' => 'DNA polymerase Ⅰ',
},
{
'item_name' => 'this enzyme produces the majority of DNA polymer during DNA replications',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA ligase',
'single-strand binding proteins',
'DNA polymerase Ⅰ',
'DNA polymerase Ⅲ',
],
'correct' => 'DNA polymerase Ⅲ',
},
{
'item_name' => 'this enzyme is responsible for the synthesis of most of the bacterial chromosome',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA topoisomerase',
'primase',
'DNA ligase',
'DNA polymerase Ⅲ',
],
'correct' => 'DNA polymerase Ⅲ',
},
{
'item_name' => 'this enzyme synthesizes DNA on both the leading strand and the lagging strand',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA topoisomerase',
'DNA ligase',
'single-strand binding proteins',
'DNA polymerase Ⅲ',
],
'correct' => 'DNA polymerase Ⅲ',
},
{
'item_name' => 'this enzyme is responsible for synthesizing the entire leading strand',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA topoisomerase',
'DNA polymerase Ⅰ',
'DNA polymerase Ⅲ',
'DNA sliding clamp',
],
'correct' => 'DNA polymerase Ⅲ',
},
{
'item_name' => 'this enzyme is a ring that tethers certain enzymes to DNA',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'primase',
'DNA ligase',
'DNA polymerase Ⅰ',
'DNA sliding clamp',
],
'correct' => 'DNA sliding clamp',
},
{
'item_name' => 'this enzyme prevents certain enzymes from falling off of the DNA',
'plural_choice' => 'DNA replication enzymes',
'singular_item' => 'function',
'choices' => [
'DNA helicase',
'DNA ligase',
'DNA polymerase Ⅰ',
'DNA sliding clamp',
],
'correct' => 'DNA sliding clamp',
},
);
#==========================================================
# SELECT RANDOM QUESTION
#==========================================================
my $local_random = PGrandom->new();
$local_random->srand($problemSeed);
$question_idx = $local_random->random(0, scalar(@questions_data) - 1, 1);
$q = $questions_data[$question_idx];
$item_name = $q->{'item_name'};
$plural_choice = $q->{'plural_choice'};
$singular_item = $q->{'singular_item'};
@choices = @{ $q->{'choices'} };
$correct = $q->{'correct'};
#==========================================================
# SHUFFLE CHOICES (SEED-STABLE)
#==========================================================
my @choice_indices = (0 .. $#choices);
my @choice_shuffled = ();
while (@choice_indices) {
my $pick = $local_random->random(0, $#choice_indices, 1);
push @choice_shuffled, splice(@choice_indices, $pick, 1);
}
@choices = @choices[@choice_shuffled];
#==========================================================
# BUILD RADIO BUTTONS
#==========================================================
$rb = RadioButtons(
[@choices],
$correct,
labels => 'ABC',
displayLabels => 1,
randomize => 0,
separator => '',
);
#==========================================================
# PGML
#==========================================================
BEGIN_PGML
Which one of the following [$plural_choice]* corresponds to the [$singular_item]* [$item_name]*?
[_]{$rb}
END_PGML
BEGIN_PGML_SOLUTION
The correct answer is: [$correct]*
END_PGML_SOLUTION
ENDDOCUMENT();