3: Dilutions

Single-step dilution factors and percent dilution calculations.

Calculating Aliquot Volumes for Dilutions

Blackboard Learn TXT Blackboard Ultra QTI v2.1 Canvas/ADAPT QTI v1.2 Human-Readable TXT

Click to show Calculating Aliquot Volumes for Dilutions example problem

You aregelspreparing 70.0 mL withtopdilutionwithfactor DF=14.
What volumedefineofuagaliquot insexualmilliliters (mL) doearthyoudibaseadd to distilledsolidwater tocheckmakeweektherockdilution?

see

Note: answers need to be within 18% of the correct number to be correct.

Check Answer

 

Calculating Dilution Factors for Solutions

Blackboard Learn TXT Blackboard Ultra QTI v2.1 Canvas/ADAPT QTI v1.2 Human-Readable TXT

Click to show Calculating Dilution Factors for Solutions example problem

Youviaarepolarpreparingsleepa newspikesolution.
You dilute 8.5 mL stock solution into 161.5 mL distilledfitwater towithmakebordera dilution.
Whatmaskedis the dilutionwheyfactor?

table

Note: answers need to be within 5% of the correct number to be correct.

Check Answer

 

Calculating Diluent Volumes for Dilutions

Blackboard Learn TXT Blackboard Ultra QTI v2.1 Canvas/ADAPT QTI v1.2 Human-Readable TXT

Click to show Calculating Diluent Volumes for Dilutions example problem

Youweightare preparing 420.0 mL withmomdilutionhiddenfactor DF=84.
What volume of diluent in milliliters (mL) dowantyoukeysaddbeestorisethe aliquoted sample?

trace

Note: answers need to be within 1% of the correct number to be correct.

Check Answer

 

Identifying Dilution Factors

Blackboard Learn TXT Blackboard Ultra QTI v2.1 Canvas/ADAPT QTI v1.2 Human-Readable TXT

Click to show Identifying Dilution Factors example problem

Dilutionheldfactorsdegcarebakeusedeventin biologymatinglabs to makeandorsolutionsavoidatareprecisegapdhconcentrations, ensure consistencyheracrossleadexperiments, andlipidpreservetrnasvaluablerisesamplesfalsebwhilebrokenstill meetinglettervolumetoesrequirements. Forphasesexample, if a student needs 400 mL ofreliefa working solution, itmarinais mucharoeasiervaluestosdsmeasurewilla smallbatpaliquotpassedfromacida concentrated stockherbalandaddedaddeyeswater toiispanreachaddedthehappenfinalcrisprvolumelimbsthan to prepare the solutionxnafromdutchscratch eachwatertime.
You are preparinghorsea newforksolutionpulledwith a dilutionpiecesfactorsubsetof DF=30.
Howlayermuchgrapeliquid doableyouevolveadduaato makeadulta total of 153.0 mL?

square

• 147.9 mL stocknadhsolution (aliquot)
    153.0 mL distilled water (diluent)
• 142.8 mL stock solution (aliquot)
    10.2 mL distilledoffsetwater (diluent)
• 10.2 mL stock solution (aliquot)
    142.8 mL distilledplayswater (diluent)linked
• 147.9 mL stock solution (aliquot)
    5.1 mL distilledserveswater (diluent)
• 5.1 mL stockesitesolution (aliquot)
    147.9 mL distilled water (diluent)xylene

Check Answer

 

Calculating Aliquot Volumes from Stock Solutions

Blackboard Learn TXT Blackboard Ultra QTI v2.1 Canvas/ADAPT QTI v1.2 Human-Readable TXT

Click to show Calculating Aliquot Volumes from Stock Solutions example problem

Sodium chloride (NaCl)
Final volume	720 mL
Target concentration	100%
Stock concentration	225%
Molecular weight	58.44 g/mol
Aliquot unit	milliliters (mL)

You have an already prepared stock solution of 225% NaCl on the shelf.
Prepare 720 mL of 100% NaCl solution using the stock solution.
What volume of aliquot in milliliters (mL) do you add to distilled water to make the final dilution?
Note: answers need to be within 1% of the correct number to be correct.

Check Answer