5: Protein Purification
Students choose purification methods for proteins based on size, charge, and binding affinity, and interpret results from gel filtration, ion exchange, SDS-PAGE, and isoelectric focusing.
LibreTexts reference: Unit 1, Chapter 5: Protein Purification 
5.1 Cell Disruption Techniques from Descriptions
Matching
Remember ➀Factual ➀
5.2 Column Chromatography Types from Descriptions
Matching
Remember ➀Conceptual ➁
5.3 Protein and Nucleic Acid Gel Electrophoresis Components
Matching
Remember ➀Factual ➀
5.4 Cell Disruption Techniques from Descriptions
WOMC
Remember ➀Factual ➀
5.5 Column Chromatography Types from Descriptions
WOMC
Remember ➀Conceptual ➁
5.6 Protein and Nucleic Acid Gel Electrophoresis Components
WOMC
Understand ➁Factual ➀
5.7 Protein Net Charge at a Given pH
MC
Apply ➂Conceptual ➁
5.8 Protein Migration Direction in Isoelectric Focusing
MC
Apply ➂Conceptual ➁
5.9 Protein Molecular Weight from an SDS-PAGE Ladder
NUM
Apply ➂Procedural ➂
5.10 Protein Molecular Weight from SDS-PAGE Migration
NUM
Apply ➂Procedural ➂
5.11 Isoelectric Point from pKa Values
MC
Apply ➂Procedural ➂