Skip to content

Biology Problems OER

04: Manipulation of Nucleic Acids

4: Manipulation of Nucleic Acids

Restriction enzymes, gel electrophoresis, and PCR.

Amplicon Copy Number After PCR Rounds

MC Apply Procedural

DNA Fragment Migration in Agarose Gel Electrophoresis (Closest/Farthest)

MC Understand Conceptual

DNA Fragment Size from Agarose Gel Migration Data

NUM Apply Procedural

Inverse PCR Primer Selection (15 nt)

MC Apply Procedural

Restriction Fragment Sizes from Linear DNA Maps (12 kb, 3 Sites, Isolated Fragment)

MA Apply Procedural

Nested PCR Primer Pair Selection (24 nt)

MC Apply Procedural

Restriction Enzyme Overhang Sequences

MC Apply Procedural

Restriction Enzyme Cut Types (5', 3', or Blunt)

MC Understand Conceptual

Palindromic DNA Sequence Completion

Matching Apply Procedural

PCR Primer Pair Selection from Double-Stranded DNA (36 bp Template, 9-nt Primers)

MC Apply Procedural