5: Cloning and Sequencing

Gene cloning, DNA sequencing methods, and RT-qPCR.

Calculating Fold Change from RT-qPCR Data

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	Untreated Cells		Cells Treated with Experimental Drug
	House Keeping Gene	Gene of Interest	House Keeping Gene	Gene of Interest
Well 1	17.8	21.3	17.9	23.0
Well 2	17.8	21.0	16.6	22.7
Well 3	17.6	21.3	17.1	23.9
mean Ct	17.7	21.2	17.2	23.2
ΔCt
ΔΔCt

Given the data in the table above calculate the fold change (2^|ΔΔCt|) value for the effect of the drug on the cells.
Note: answers need to be within 1% of the correct number to be correct.

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