6: DNA Replication

Replication machinery, origins, forks, and fidelity mechanisms.

Matching DNA Replication Enzymes to Functions (BIOL 301)

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Match each of the following DNA replication enzymes with their corresponding functions.
Note: Each choice will be used exactly once.

	Your Choice	Prompt
	Drop Your Choice Here	1. DNA ligase
	Drop Your Choice Here	2. DNA polymerase Ⅰ
	Drop Your Choice Here	3. DNA topoisomerase
	Drop Your Choice Here	4. single-strand binding proteins

Drag one of the choices below:

• A. this enzyme removes RNA primers and fills the gaps with DNA between Okazaki fragments
• B. this protein holds the DNA strands apart while they are being replicated
• C. this enzyme releases the supercoiling tension in the DNA double helix
• D. this enzyme joins Okazaki fragments together to create one continuous DNA strand

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Matching DNA Replication Enzymes to Functions

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Click to show Matching DNA Replication Enzymes to Functions example problem

Match each of the following DNA replication enzymes with their corresponding functions.
Note: Each choice will be used exactly once.

	Your Choice	Prompt
	Drop Your Choice Here	1. single-strand binding proteins
	Drop Your Choice Here	2. DNA sliding clamp
	Drop Your Choice Here	3. DNA helicase
	Drop Your Choice Here	4. DNA gyrase

Drag one of the choices below:

• A. this enzyme releases the supercoiling tension in the DNA double helix
• B. this enzyme is a ring that tethers certain enzymes to DNA
• C. this enzyme unwinds the parental DNA double helix
• D. this protein holds the DNA strands apart while they are being replicated

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True/False Statements About Bacterial DNA Replication (BIOL 301)

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Which one of the following statements is TRUE of DNA replication in bacteria?

• A. primase is a type of DNA polymerase that creates a DNA primer
• B. all DNA polymerases add dNTP monomers to the terminal 3′-OH of the newly synthesized strand
• C. single-strand binding proteins stabilize the DNA double helix conformation of the newly synthesized DNA
• D. DNA helicase releases the supercoiling tension in the DNA double helix
• E. the leading strand is replicated piecewise, while the lagging strand is replicated continuously

Check Answer

 

True/False Statements About Bacterial DNA Replication

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Which one of the following statements is FALSE concerning DNA replication in bacteria?

• A. Okazaki fragments are DNA fragments synthesized on the lagging strand
• B. single-strand binding proteins stabilize the open conformation of the unwound DNA
• C. primase is a type of RNA polymerase that creates an RNA primer
• D. the leading strand is replicated continuously, while the lagging strand is replicated piecewise
• E. DNA gyrase removes the RNA primers at the beginning of each Okazaki fragment and replaces them with DNA

Check Answer

 

True/False Statements About Leading and Lagging Strands

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Which one of the following statements is TRUE of difference between the leading strand and the lagging strand in DNA replication?

• A. Okazaki fragments are DNA fragments synthesized on the leading strand, but NOT on the lagging strand
• B. both the leading strand and the lagging strand are synthesized by adding nucleotides to the 3′ end of the growing strand
• C. both the leading strand and the lagging strand require DNA primers
• D. both the leading strand and the lagging strand are replicated continuously
• E. the leading strand is synthesized in the 3′→5′ direction of the template strand,
  while the lagging strand is synthesized in the 5′→3′ direction of the template strand

Check Answer

 

True/False Statements About DNA Polymerase Proofreading

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Which one of the following statements is FALSE about proofreading by DNA polymerase?

• A. RNA polymerase can start synthesis without primer
• B. proofreading allows DNA polymerase to remove mismatched nucleotides in the primase direction
• C. the 3′→5′ exonuclease polymerase domain is required for proofreading
• D. Taq DNA polymerase does NOT have proofreading capability
• E. DNA polymerase requires a primer to start synthesis

Check Answer

 

DNA Replication Enzymes from Functional Descriptions (BIOL 301)

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Which one of the following DNA replication enzymes correspond to the function 'this enzyme joins Okazaki fragments together to create one continuous DNA strand'.

• A. DNA helicase
• B. DNA ligase
• C. DNA topoisomerase
• D. primase

Check Answer

 

DNA Replication Enzymes from Functional Descriptions

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Which one of the following DNA replication enzymes correspond to the function 'this enzyme separates the two strands of the DNA double helix'.

• A. DNA helicase
• B. DNA polymerase Ⅰ
• C. DNA polymerase Ⅲ
• D. primase

Check Answer