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All Biology Problems

Browse the generated problem sets by subject and topic. Use your browser's Find command to search all problem-set titles on this page. For sortable results and subject, topic, type, and Bloom filters, use the Question Finder.

501 problem sets

Bloom's scales

Two dimensions describe the assessed task. Numbers identify categories, not difficulty ratings.

Cognitive Process Dimension

Remember ➀
Retrieve relevant knowledge from memory by recognizing or recalling.
Understand ➁
Construct meaning by interpreting, classifying, summarizing, comparing, or explaining.
Apply ➂
Carry out or use a procedure in a given situation.
Analyze ➃
Separate material into parts and identify their relationships and overall structure.
Evaluate ➄
Make judgments using criteria and standards through checking or critique.
Create ➅
Combine elements into a coherent new whole through generating, planning, or producing.

Knowledge Dimension

Factual ➀
Knowledge of basic elements, terminology, and specific details.
Conceptual ➁
Knowledge of categories, principles, theories, models, and how they relate.
Procedural ➂
Knowledge of subject-specific skills, algorithms, techniques, methods, and when to use them.
Metacognitive ➃
Knowledge of learning strategies, thinking tasks, and one's own strengths and limitations.

Based on Anderson and Krathwohl (2001), chapters 4-5. Iowa State Bloom's taxonomy guide.

Biochemistry

Life Molecules

NameQuestion typeBloom classification
Biochemical Functional Groups from DescriptionsMatchingRemember ➀Factual ➀
Chemical Bond Types from CharacteristicsMatchingUnderstand ➁Conceptual ➁
Macromolecule Classification (Names and Properties)MatchingRemember ➀Factual ➀
Macromolecule Classification (Molecule Names)MatchingUnderstand ➁Conceptual ➁
Biochemical Functional Groups from DescriptionsWOMCRemember ➀Factual ➀
Chemical Bond Types from CharacteristicsWOMCUnderstand ➁Conceptual ➁
Macromolecule Classification (Names and Properties)WOMCUnderstand ➁Conceptual ➁
Macromolecule Classification (Molecule Names)WOMCUnderstand ➁Conceptual ➁
Hydrophobic Compounds from Molecular FormulasMCUnderstand ➁Conceptual ➁
Macromolecule Types from Chemical StructuresMCUnderstand ➁Conceptual ➁

Water and pH

Amino Acids

NameQuestion typeBloom classification
Amino Acid Properties (Structure Diagrams Included)MatchingRemember ➀Factual ➀
Amino Acid PropertiesMatchingRemember ➀Factual ➀
Amino Acid Properties (Structure Diagrams Included)WOMCRemember ➀Factual ➀
Amino Acid PropertiesWOMCRemember ➀Factual ➀
Alanine Charge States at Different pH ValuesMCApply ➂Conceptual ➁
Alpha-Amino Acid Identification from StructuresMCUnderstand ➁Conceptual ➁
Histidine Protonation States at Specific pH ValuesMCApply ➂Conceptual ➁
Ionic Bond Formation in Amino Acid Side ChainsMCUnderstand ➁Conceptual ➁
Amino Acids from Chemical Structures (4 Choices)MatchingRemember ➀Factual ➀
Dipeptide Sequences from StructuresFiBApply ➂Procedural ➂
Dipeptide Sequences from StructuresMCApply ➂Factual ➀
Tripeptide Sequences from StructuresMCApply ➂Factual ➀
Tetrapeptide Sequences from StructuresMCApply ➂Procedural ➂
Tetrapeptide Net Charge from pKa Values (5 Choices)MCApply ➂Procedural ➂
Amino Acids from Chemical StructuresFiBUnderstand ➁Factual ➀
Amino Acids from Chemical Structures (7 Choices)MCUnderstand ➁Factual ➀
Pentapeptide Sequences from Structures (Wordle Words)FiBApply ➂Factual ➀

Protein Structure

NameQuestion typeBloom classification
Levels of Protein StructureMatchingRemember ➀Conceptual ➁
Levels of Protein StructureWOMCRemember ➀Conceptual ➁
Hydrogen Bonds in Alpha-Helix StructuresMAApply ➂Conceptual ➁
Hydrogen Bonds in Alpha-Helix StructuresMCApply ➂Conceptual ➁

Protein Purification

NameQuestion typeBloom classification
Cell Disruption Techniques from DescriptionsMatchingRemember ➀Factual ➀
Column Chromatography Types from DescriptionsMatchingRemember ➀Conceptual ➁
Protein and Nucleic Acid Gel Electrophoresis ComponentsMatchingRemember ➀Factual ➀
Cell Disruption Techniques from DescriptionsWOMCRemember ➀Factual ➀
Column Chromatography Types from DescriptionsWOMCRemember ➀Conceptual ➁
Protein and Nucleic Acid Gel Electrophoresis ComponentsWOMCUnderstand ➁Factual ➀
Protein Net Charge at a Given pHMCApply ➂Conceptual ➁
Protein Migration Direction in Isoelectric FocusingMCApply ➂Conceptual ➁
Protein Molecular Weight from an SDS-PAGE LadderNUMApply ➂Procedural ➂
Protein Molecular Weight from SDS-PAGE MigrationNUMApply ➂Procedural ➂
Isoelectric Point from pKa ValuesMCApply ➂Procedural ➂

Thermodynamics

NameQuestion typeBloom classification
Energy-Requiring and Energy-Releasing ProcessesMatchingRemember ➀Conceptual ➁
Enzyme Catalysis Terms from DefinitionsMatchingRemember ➀Factual ➀
Chemical ReactionsT/F Statements (MC)Remember ➀Conceptual ➁
Enzyme Cofactor IdentificationT/F Statements (MC)Understand ➁Conceptual ➁
Enzyme Equilibrium and KineticsT/F Statements (MC)Remember ➀Conceptual ➁
Gibbs Free Energy (Delta G = Delta H - T Delta S)T/F Statements (MC)Remember ➀Conceptual ➁
Potential and Kinetic Energy ExamplesT/F Statements (MC)Understand ➁Conceptual ➁
Thermodynamics and KineticsT/F Statements (MC)Remember ➀Conceptual ➁
Enzyme Naming PatternsT/F Statements (MC)Understand ➁Conceptual ➁
Energy-Requiring and Energy-Releasing ProcessesWOMCRemember ➀Conceptual ➁
Enzyme Catalysis Terms from DefinitionsWOMCRemember ➀Factual ➀
Biochemical vs Chemical Standard Free Energy StatesMCRemember ➀Factual ➀
Exergonic and Endergonic Reaction ClassificationMCRemember ➀Conceptual ➁
Gibbs Free Energy Change and Equilibrium Constant RelationshipsMCUnderstand ➁Conceptual ➁
Gibbs Free Energy Equation Symbols and MeaningsMatchingRemember ➀Factual ➀
Thermodynamics Law StatementsMCRemember ➀Conceptual ➁
First and Second Laws of Thermodynamics for Different SystemsMCRemember ➀Conceptual ➁

Enzyme Kinetics

NameQuestion typeBloom classification
Michaelis-Menten KineticsT/F Statements (MC)Remember ➀Conceptual ➁
Michaelis-Menten Constant (Km) from Enzyme Activity DataMCApply ➂Procedural ➂
Enzyme Activity Changes with Temperature and pHMCApply ➂Conceptual ➁
Enzyme Selection for Given Temperature and pHMCApply ➂Conceptual ➁
Optimal Temperature and pH for Enzyme ActivityMCApply ➂Conceptual ➁

Enzyme Inhibition

NameQuestion typeBloom classification
Enzyme Catalytic StrategiesMatchingRemember ➀Conceptual ➁
Enzyme Catalytic StrategiesT/F Statements (MC)Remember ➀Conceptual ➁
Chymotrypsin FunctionT/F Statements (MC)Remember ➀Factual ➀
Enzyme InhibitorsT/F Statements (MC)Remember ➀Conceptual ➁
Enzyme Catalytic StrategiesWOMCRemember ➀Conceptual ➁
Cleavage Sites for Chymotrypsin Digestion of PeptidesMCApply ➂Procedural ➂
Enzyme Inhibition Type from Metabolic Pathway DescriptionsMCUnderstand ➁Conceptual ➁
Enzyme Inhibition Type from Enzyme Activity DataMCUnderstand ➁Conceptual ➁

Enzyme Allostery

NameQuestion typeBloom classification
Allosteric Enzyme Models from Conformational DescriptionsMCRemember ➀Conceptual ➁
Metabolic Regulation in Converging PathwaysMCAnalyze ➃Conceptual ➁
Metabolic Regulation in Diverging PathwaysMCAnalyze ➃Conceptual ➁
Hemoglobin Oxygen Affinity Under Different ConditionsMCRemember ➀Conceptual ➁
Allosteric Enzymes in Metabolic PathwaysMCUnderstand ➁Conceptual ➁

Carbohydrates

Nucleic Acids

NameQuestion typeBloom classification
DNA Structure (Core Set)T/F Statements (MC)Remember ➀Factual ➀
DNA Structure (Expanded Set)T/F Statements (MC)Remember ➀Factual ➀
DNA Structure from Rosalind Franklin's Diffraction DataT/F Statements (MC)Remember ➀Factual ➀
G-U Wobble Base PairsT/F Statements (MC)Remember ➀Factual ➀
DNA Melting Temperature (Tm)T/F Statements (MC)Remember ➀Conceptual ➁
DNA Melting Temperature (Factors That Increase Tm)T/F Statements (MC)Understand ➁Conceptual ➁
DNA Melting Temperature (Factors That Decrease Tm)T/F Statements (MC)Understand ➁Conceptual ➁
Nucleotide ComponentsT/F Statements (MC)Remember ➀Factual ➀
RNA and DNA Differences (Core Set)T/F Statements (MC)Remember ➀Factual ➀
RNA and DNA Differences (Helix Forms and Charge Included)T/F Statements (MC)Remember ➀Factual ➀
DNA Base Composition Using Chargaff's Rules (5 Choices)MCApply ➂Procedural ➂
Complementary DNA Sequences (Directionless)MCApply ➂Procedural ➂
DNA Melting Temperature Extremes from Sequence (12 Bases)MCApply ➂Conceptual ➁
Purine Names from Chemical Structures (3 Choices)MatchingRemember ➀Factual ➀
Pyrimidine Names from Chemical Structures (4 Choices)MatchingRemember ➀Factual ➀
Palindromic DNA Sequence CompletionMatchingApply ➂Procedural ➂
mRNA Sequences from DNA Template Strands (9 Nucleotides, Directionless)FiBApply ➂Procedural ➂
mRNA Sequences from DNA Coding and Template Strands (9 Nucleotides, 5'/3' Labels)FiBApply ➂Procedural ➂
mRNA Sequences from DNA Template Strands (9 Nucleotides, 5'/3' Labels)MCApply ➂Procedural ➂
Peptide Sequences from mRNA Using the Genetic Code (10 Amino Acids)FiBApply ➂Procedural ➂

Lipids

NameQuestion typeBloom classification
Fatty Acid Delta Notations from StructuresMCApply ➂Procedural ➂
Fatty Acid Omega Notations from StructuresMCApply ➂Procedural ➂
Fatty Acid Double-Bond Positions in Delta NotationMCApply ➂Procedural ➂
Fatty Acid Double-Bond Positions in Omega NotationMCApply ➂Procedural ➂
Fatty Acid Chain Length and Number of Double BondsMCUnderstand ➁Factual ➀
Lipids by Molecular FormulaMCUnderstand ➁Conceptual ➁

Membranes and Membrane Proteins

NameQuestion typeBloom classification
Fluid Mosaic ModelT/F Statements (MC)Remember ➀Conceptual ➁
Membrane Permeability to Ions and MoleculesT/F Statements (MC)Understand ➁Conceptual ➁
Membrane Protein FunctionsT/F Statements (MC)Remember ➀Factual ➀

Human Senses

NameQuestion typeBloom classification
Sensory Modalities from Receptor Cell TypesMatchingRemember ➀Factual ➀
Sensory Signal Transduction Components from FunctionsMatchingRemember ➀Factual ➀
Taste Qualities from StimuliMatchingRemember ➀Factual ➀
Chemosensation (Smell and Taste)T/F Statements (MC)Remember ➀Conceptual ➁
Sensory Coding (Smell vs. Taste)T/F Statements (MC)Remember ➀Conceptual ➁
Vision and Hearing TransductionT/F Statements (MC)Remember ➀Conceptual ➁
Sensory Modalities from Receptor Cell TypesWOMCRemember ➀Factual ➀
Sensory Signal Transduction Components from FunctionsWOMCRemember ➀Factual ➀
Taste Qualities from StimuliWOMCRemember ➀Factual ➀

Sugar Metabolism

NameQuestion typeBloom classification
Cellular Respiration Stages from StatementsMatchingRemember ➀Factual ➀
Glycolysis Intermediate OrderingORDAnalyze ➃Conceptual ➁
Glycolysis Intermediate OrderingSequence MCAnalyze ➃Conceptual ➁
Cellular Respiration Stages from StatementsWOMCRemember ➀Factual ➀

Photosynthesis

NameQuestion typeBloom classification
Optimal Wavelengths for Pigment AbsorptionMCApply ➂Conceptual ➁

Genetics

Genetic Disorders

NameQuestion typeBloom classification
Genetic DisordersMatchingRemember ➀Factual ➀
Genetic DisordersWOMCRemember ➀Factual ➀
Metabolic Pathway Mutant Growth Rescue by Nutrient Supplementation (5 Metabolites)MAApply ➂Conceptual ➁

DNA Structure

NameQuestion typeBloom classification
DNA Structure (Core Set)T/F Statements (MC)Remember ➀Factual ➀
English Palindrome IdentificationT/F Statements (MC)Understand ➁Factual ➀
Nucleotide ComponentsT/F Statements (MC)Remember ➀Factual ➀
RNA and DNA Differences (Helix Forms and Charge Included)T/F Statements (MC)Remember ➀Factual ➀
DNA Base Composition Using Chargaff's Rules (5 Choices)MCApply ➂Procedural ➂
Restriction Fragment Sizes from Circular DNA Maps (10 kb, 2 Sites)MAApply ➂Procedural ➂
Restriction Fragment Sizes from Circular DNA Maps (12 kb, 3 Sites)MAApply ➂Procedural ➂
Restriction Fragment Sizes from Circular DNA Maps (16 kb, 3 Sites)MAApply ➂Procedural ➂
Complementary DNA Sequences (Directionless)MCApply ➂Procedural ➂
Complementary DNA Sequences (5'/3' Labels)MCApply ➂Procedural ➂
Restriction Fragment Sizes from Linear DNA Maps (10 kb, 2 Sites, Isolated Fragment)MAApply ➂Procedural ➂
Restriction Fragment Sizes from Linear DNA Maps (10 kb, 3 Sites, Internal Segment)MAApply ➂Procedural ➂
Restriction Fragment Sizes from Linear DNA Maps (12 kb, 3 Sites, Isolated Fragment)MAApply ➂Procedural ➂
Restriction Fragment Sizes from Linear DNA Maps (12 kb, 3 Sites, Internal Segment)MAApply ➂Procedural ➂
Restriction Fragment Sizes from Linear DNA Maps (16 kb, 4 Sites, Isolated Fragment)MAApply ➂Procedural ➂
Restriction Fragment Sizes from Linear DNA Maps (16 kb, 4 Sites, Internal Segment)MAApply ➂Procedural ➂
Restriction Enzyme Overhang SequencesMCApply ➂Procedural ➂
Restriction Enzyme Cut Types (5', 3', or Blunt)MCUnderstand ➁Conceptual ➁
Restriction Enzyme Cut Types (Blunt or Sticky)MCUnderstand ➁Conceptual ➁
Palindromic DNA Sequence CompletionMatchingApply ➂Procedural ➂

DNA Profiling

Mendelian Genetics

NameQuestion typeBloom classification
Genetics Terms from DefinitionsMatchingRemember ➀Factual ➀
Mendelian Cross Terms from DescriptionsMatchingRemember ➀Factual ➀
Mendel's Four Principles of Genetics from DescriptionsMatchingRemember ➀Conceptual ➁
Mendelian Genetics Terms from DefinitionsMatchingRemember ➀Factual ➀
Monohybrid Cross Parental Genotypes from Progeny OutcomesMatchingApply ➂Conceptual ➁
Genetics Terms from DefinitionsWOMCRemember ➀Factual ➀
Mendelian Cross Terms from DescriptionsWOMCRemember ➀Factual ➀
Mendel's Four Principles of Genetics from DescriptionsWOMCRemember ➀Conceptual ➁
Mendelian Genetics Terms from DefinitionsWOMCRemember ➀Factual ➀
Monohybrid Cross Parental Genotypes from Progeny OutcomesWOMCApply ➂Conceptual ➁
Parental Genotype Crosses from Monohybrid Offspring RatiosMCApply ➂Conceptual ➁
Unknown Parent Genotype from Monohybrid Offspring CountsMCApply ➂Conceptual ➁
Punnett Squares from Monohybrid Cross DescriptionsMCApply ➂Procedural ➂
Unique Offspring Genotype Counts from Parental Genotypes (4 Genes, With Hint)MCApply ➂Procedural ➂
Unique Offspring Genotypes from Four-Gene Crosses (With Hint)MCApply ➂Procedural ➂
Offspring Phenotype Counts from Genetic Crosses (4 Genes, With Hint)MCApply ➂Procedural ➂
Unique Offspring Phenotypes from Four-Gene Crosses (With Hint)MCApply ➂Procedural ➂
Unique Gamete Counts from a Parental Genotype (4 Genes, With Hint)MCApply ➂Procedural ➂
Unique Gametes from Four-Gene Genotypes (With Hint)MCApply ➂Procedural ➂

Gene Interactions

Chromosomal Inheritance

NameQuestion typeBloom classification
Meiosis Prophase I Stages from DescriptionsMatchingRemember ➀Factual ➀
Meiosis Terms from DefinitionsMatchingRemember ➀Factual ➀
Sex Determination Systems from DescriptionsMatchingRemember ➀Conceptual ➁
Mitosis and MeiosisT/F Statements (MC)Remember ➀Conceptual ➁
Meiosis Prophase I Stages from DescriptionsWOMCRemember ➀Factual ➀
Meiosis Terms from DefinitionsWOMCRemember ➀Factual ➀
Sex Determination Systems from DescriptionsWOMCRemember ➀Conceptual ➁
Combined Autosomal Dominant and X-Linked Recessive Inheritance (Both Disorders)MCApply ➂Procedural ➂
Combined Autosomal Dominant and X-Linked Recessive Inheritance (Varied Outcomes)MCApply ➂Procedural ➂
Parent Genotypes in X-Linked Recessive CrossesMCApply ➂Conceptual ➁
Pedigree Identification from Inheritance Patterns (Easy)MCUnderstand ➁Conceptual ➁
Inheritance Patterns from Pedigrees (Easy)MatchingUnderstand ➁Conceptual ➁
Inheritance Patterns from Pedigrees (Bonus)MCUnderstand ➁Conceptual ➁
Inheritance Patterns from Pedigrees (Easy)MCUnderstand ➁Conceptual ➁
Inheritance Patterns from Pedigrees (Medium)MCUnderstand ➁Conceptual ➁
Inheritance Patterns from Pedigrees (Rigorous)MCUnderstand ➁Conceptual ➁
X-Linked Eye Color InheritanceMCApply ➂Conceptual ➁
X-Linked Coat Color ProbabilityMCApply ➂Procedural ➂

Chi Square Analysis

Gene Mapping

NameQuestion typeBloom classification
Gene Order and Pairwise Recombination Distances from Four-Point Test CrossesMulti-FiBApply ➂Procedural ➂
Gene-to-Centromere Distance from Ordered TetradsMCApply ➂Procedural ➂
Gene Order and Map Distances from Unordered TetradsMulti-FiBApply ➂Procedural ➂
Single Genetic Distance from Unordered Three-Gene Tetrads (5 Choices)MCApply ➂Procedural ➂
Genetic Distance from Unordered Two-Gene Tetrads (6 Choices)MCApply ➂Procedural ➂
Linkage Tests from Unordered Two-Gene TetradsMCApply ➂Procedural ➂
Gene Order and Map Distances from Three-Point Test CrossesMulti-FiBApply ➂Procedural ➂
Crossover Interference from Three-Point Test Cross Data (6 Choices)MCApply ➂Procedural ➂
Crossover Interference from Three-Point Test Cross DataNUMApply ➂Procedural ➂
Genetic Distance for a Gene Pair from Three-Point Test CrossesMCApply ➂Procedural ➂
Genetic Distance for a Gene Pair from Three-Point Test CrossesNUMApply ➂Procedural ➂
Double-Crossover Genotypes in Three-Point Test CrossesMAApply ➂Procedural ➂
Recombinant Genotypes for a Gene Pair from Three-Point Test Cross DataMAApply ➂Procedural ➂
Three-Point Test Cross: Parental Genotypes from Progeny CountsMAAnalyze ➃Procedural ➂
Cis or Trans Allele Configuration from Two-Point Test Cross DataMCAnalyze ➃Conceptual ➁
Genetic Distance from Two-Point Test Cross Progeny Counts (6 Choices)MCApply ➂Procedural ➂
Genetic Distance from Two-Point Test Cross ProgenyNUMApply ➂Procedural ➂
Parental Genotypes from Two-Point Test Cross DataMAAnalyze ➃Conceptual ➁
Recombinant Genotypes from Two-Point Test Cross DataMAAnalyze ➃Conceptual ➁

Chromosomal Disorders

NameQuestion typeBloom classification
Chromosome Structure Alterations from DescriptionsMatchingRemember ➀Factual ➀
Chromosome Shapes from DescriptionsMatchingRemember ➀Factual ➀
Chromosome Structure Alterations from DescriptionsWOMCRemember ➀Factual ➀
Chromosome Shapes from DescriptionsWOMCRemember ➀Factual ➀
Chromosome Alterations from DiagramsMatchingApply ➂Conceptual ➁
Chromosome Alteration Diagrams from NamesMCApply ➂Conceptual ➁
Chromosome Alterations from DiagramsMCApply ➂Conceptual ➁
Aneuploidy from Cytogenetic NotationMCUnderstand ➁Factual ➀
Proximal and Distal Chromosome BandsMCApply ➂Factual ➀
Cytogenetic Notation from Disorder DescriptionsMCApply ➂Procedural ➂
Chromosome Rearrangements from Cytogenetic NotationMCUnderstand ➁Factual ➀
Gene Order from Deletion Mutants (Easy, 4 Genes, Random Labels, Deletion List, With Hint)MCAnalyze ➃Procedural ➂
Gene Order from Deletion Mutants (Hard, 8 Genes, Random Labels, Deletion Table)MCAnalyze ➃Procedural ➂
Gene Order from Deletion Mutants (Bonus, 12 Genes, Random Labels, Deletion List)FiBAnalyze ➃Procedural ➂
Gene Order from Deletion Mutants (Medium, 5-6 Genes, Word Labels, Deletion Table)FiBAnalyze ➃Procedural ➂
Chromosomal Translocation Outcomes (Text)MCApply ➂Conceptual ➁
Chromosomal Translocation Outcomes (Chromosome Diagrams)MCApply ➂Conceptual ➁
Gamete Chromosome Numbers in PolyploidsMCApply ➂Procedural ➂
Monoploid and Haploid Numbers from Chromosome CountsMCApply ➂Procedural ➂
Gametes from Robertsonian TranslocationMCApply ➂Conceptual ➁
Gametes from Balanced Translocation SegregationMAApply ➂Conceptual ➁

Population Genetics

NameQuestion typeBloom classification
Population Genetics Terms from DefinitionsMatchingRemember ➀Factual ➀
Hardy-Weinberg Equilibrium ConditionsT/F Statements (MC)Remember ➀Conceptual ➁
Population Genetics Terms from DefinitionsWOMCRemember ➀Factual ➀
Hardy-Weinberg Chi-Square Test ConclusionsMCEvaluate ➄Procedural ➂
Hardy-Weinberg Variables from Population DataMCUnderstand ➁Conceptual ➁
Hardy-Weinberg Allele and Genotype Frequencies from Population DataNUMApply ➂Procedural ➂

Gene Trees

Laboratory

Measurement Basics

NameQuestion typeBloom classification
Orders of Magnitude for Given QuantitiesMCApply ➂Procedural ➂
Pipette Sizes for Given VolumesMCApply ➂Procedural ➂

Solutions

NameQuestion typeBloom classification
Solute Mass from Mass ConcentrationNUMApply ➂Procedural ➂
Mass for Weight/Volume SolutionsNUMApply ➂Procedural ➂
Mass for Solutions Using Molarity and Molecular WeightNUMApply ➂Procedural ➂
Solute Volume for Percent Volume/Volume SolutionsNUMApply ➂Procedural ➂

Dilutions

NameQuestion typeBloom classification
Aliquot Volumes for DilutionsNUMApply ➂Procedural ➂
Dilution Factor CalculationsNUMApply ➂Procedural ➂
Diluent Volumes for DilutionsNUMApply ➂Procedural ➂
Aliquot and Diluent Volumes from Dilution FactorsMCApply ➂Procedural ➂
Aliquot Volumes from Stock SolutionsNUMApply ➂Procedural ➂

Serial Dilutions

NameQuestion typeBloom classification
Aliquot Volumes for Serial DilutionsNUMApply ➂Procedural ➂
Diluent Volumes for Serial DilutionsNUMApply ➂Procedural ➂
Aliquot and Diluent Volumes for Serial DilutionsMCApply ➂Procedural ➂

Enzyme Kinetics

NameQuestion typeBloom classification
Michaelis-Menten Constant (Km) from Enzyme Activity DataMCApply ➂Procedural ➂
Enzyme Inhibition Type from Enzyme Activity DataMCUnderstand ➁Conceptual ➁

PCR and Electrophoresis

Bioinformatics

NameQuestion typeBloom classification
Degenerate Primer Codes from Sequence AlignmentsFiBApply ➂Procedural ➂
Consensus Sequences from Alignments (Easy)FiBApply ➂Procedural ➂
Consensus Sequences from Alignments (Hard)FiBApply ➂Procedural ➂
Consensus Sequences from AlignmentsMCApply ➂Procedural ➂

Molecular Biology

DNA Structure

NameQuestion typeBloom classification
DNA Structure (Expanded Set)T/F Statements (MC)Remember ➀Factual ➀
DNA Melting Temperature (Tm)T/F Statements (MC)Remember ➀Conceptual ➁
DNA Melting Temperature (Factors That Increase Tm)T/F Statements (MC)Understand ➁Conceptual ➁
DNA Melting Temperature (Factors That Decrease Tm)T/F Statements (MC)Understand ➁Conceptual ➁
Nucleotide ComponentsT/F Statements (MC)Remember ➀Factual ➀
RNA and DNA Differences (Core Set)T/F Statements (MC)Remember ➀Factual ➀
RNA and DNA Differences (Helix Forms and Charge Included)T/F Statements (MC)Remember ➀Factual ➀
DNA Base Composition Using Chargaff's Rules (5 Choices)MCApply ➂Procedural ➂
Complementary DNA Sequences (Directionless)MCApply ➂Procedural ➂
DNA Melting Temperature Extremes from Sequence (12 Bases)MCApply ➂Conceptual ➁

Manipulation of Nucleic Acids

Cloning and Sequencing

NameQuestion typeBloom classification
Gene Expression Fold Change from RT-qPCR DataNUMApply ➂Procedural ➂
Long-Distance PCRT/F Statements (MC)Remember ➀Factual ➀
PCR ComponentsT/F Statements (MC)Remember ➀Factual ➀
Efficient PCR PrimersT/F Statements (MC)Remember ➀Procedural ➂
PCR Step OrderT/F Statements (MC)Remember ➀Procedural ➂
Degenerate Primer Codes from Sequence AlignmentsFiBApply ➂Procedural ➂
Consensus Sequences from Alignments (Easy)FiBApply ➂Procedural ➂
Consensus Sequences from Alignments (Hard)FiBApply ➂Procedural ➂
Consensus Sequences from AlignmentsMCApply ➂Procedural ➂

DNA Replication

NameQuestion typeBloom classification
Bacterial DNA Replication Proteins and Functions (Topoisomerase Terminology)MatchingRemember ➀Factual ➀
Bacterial DNA Replication Proteins and Functions (Gyrase Terminology)MatchingRemember ➀Factual ➀
Bacterial DNA Replication (Topoisomerase Terminology)T/F Statements (MC)Remember ➀Factual ➀
Bacterial DNA Replication (Gyrase Terminology)T/F Statements (MC)Remember ➀Factual ➀
Leading and Lagging StrandsT/F Statements (MC)Remember ➀Conceptual ➁
DNA Polymerase ProofreadingT/F Statements (MC)Remember ➀Conceptual ➁
Bacterial DNA Replication Proteins and Functions (Topoisomerase Terminology)WOMCRemember ➀Factual ➀
Bacterial DNA Replication Proteins and Functions (Gyrase Terminology)WOMCRemember ➀Factual ➀

RNA Transcription

RNA Processing and CRISPR

NameQuestion typeBloom classification
Intron Removal MechanismsT/F Statements (MC)Remember ➀Factual ➀
mRNA Post-Transcriptional ModificationT/F Statements (MC)Remember ➀Factual ➀
Alternative Splicing PossibilitiesMCAnalyze ➃Conceptual ➁

Translation

NameQuestion typeBloom classification
Central Dogma Stages from Related TermsMatchingRemember ➀Factual ➀
G-U Wobble Base PairsT/F Statements (MC)Remember ➀Factual ➀
Translation AccuracyT/F Statements (MC)Remember ➀Conceptual ➁
Central Dogma Stages from Related TermsWOMCRemember ➀Factual ➀
Peptide Sequences from mRNA Using the Genetic Code (10 Amino Acids)FiBApply ➂Procedural ➂

Historical Experiments

Biostatistics

Descriptive Statistics

NameQuestion typeBloom classification
Measures of Center from DefinitionsMatchingRemember ➀Factual ➀
Measures of Center from DefinitionsWOMCRemember ➀Factual ➀
Descriptive Statistics Using Google SheetsMulti-FiBApply ➂Procedural ➂

Graph Interpretation

NameQuestion typeBloom classification
Box Plots from Cumulative Frequency TablesMCApply ➂Procedural ➂
Box Plots from Sorted DataMCApply ➂Procedural ➂
Box Plots from Five-Number SummaryMCApply ➂Procedural ➂
Box Plots from Unsorted Data (Even Sample Size)MCApply ➂Procedural ➂

Grouping with Z-Scores

NameQuestion typeBloom classification
Z-Scores in Google SheetsMCApply ➂Procedural ➂
Biodiversity Comparisons Using Z-ScoresMCApply ➂Procedural ➂

Hypothesis Testing Concepts

NameQuestion typeBloom classification
Hypothesis Testing Terms from DefinitionsMatchingRemember ➀Factual ➀
Statistical Hypothesis Tests from DescriptionsMatchingUnderstand ➁Procedural ➂
Statistical Hypothesis Testing DecisionsT/F Statements (MC)Remember ➀Conceptual ➁
Hypothesis Testing Terms from DefinitionsWOMCRemember ➀Factual ➀
Statistical Hypothesis Tests from DescriptionsWOMCApply ➂Procedural ➂
Errors in Null and Alternative Hypotheses for Shannon Diversity Means and VariancesMCEvaluate ➄Conceptual ➁
Null and Alternative Hypothesis Identification for Mean, Variance, and ANOVA TestsMCApply ➂Procedural ➂

Z-Tests, T-Tests, F-Tests, and ANOVA

Chi-Square Tests

NameQuestion typeBloom classification
Chi-Square Terms from DefinitionsMatchingRemember ➀Factual ➀
Null Hypothesis Rejection CriteriaT/F Statements (MC)Remember ➀Procedural ➂
Chi-Square Terms from DefinitionsWOMCRemember ➀Factual ➀

Biotechnology

Biotech Basics and Central Dogma

NameQuestion typeBloom classification
Biotechnology and Related Industries from Project Descriptions (Traditional vs. Modern)MatchingUnderstand ➁Conceptual ➁
Historical Biotechnology Periods from MilestonesMatchingRemember ➀Factual ➀
Model Organisms from Approximate Generation TimesMatchingRemember ➀Factual ➀
Model Organism Research Concepts from DescriptionsMatchingRemember ➀Conceptual ➁
Model Organism Scientific Names from Common Names and GroupsMatchingRemember ➀Factual ➀
Model Organisms by Complexity (7 Organisms)ORDUnderstand ➁Conceptual ➁
Model Organisms by Complexity (7 Organisms)Sequence MCUnderstand ➁Conceptual ➁
Fermentation Substrate IdentificationT/F Statements (MC)Remember ➀Factual ➀
mRNA Post-Transcriptional ModificationT/F Statements (MC)Remember ➀Factual ➀
Biotechnology and Related Industries from Project Descriptions (Traditional vs. Modern)WOMCUnderstand ➁Conceptual ➁
Historical Biotechnology Periods from MilestonesWOMCRemember ➀Factual ➀
Model Organisms from Approximate Generation TimesWOMCRemember ➀Factual ➀
Model Organism Research Concepts from DescriptionsWOMCRemember ➀Conceptual ➁
Model Organism Scientific Names from Common Names and GroupsWOMCRemember ➀Factual ➀
Metabolic Pathway Mutant Growth Rescue by Nutrient Supplementation (4 Metabolites)MAApply ➂Conceptual ➁
Metabolic Pathway Mutant Screens: Wild-Type Identification from Precursor Growth Patterns (4 Metabolites)MCAnalyze ➃Conceptual ➁
mRNA Sequences from DNA Template Strands (6 Nucleotides, 5'/3' Labels)MCApply ➂Procedural ➂
Genetic Code Translation of mRNA into Five-Amino-Acid Peptides (5 Choices)MCApply ➂Procedural ➂

DNA and Genomics Biotechnology

NameQuestion typeBloom classification
Cell Disruption Techniques from DescriptionsMatchingRemember ➀Factual ➀
DNA Sequencing Techniques from DescriptionsMatchingRemember ➀Factual ➀
Latin Phrases from Their MeaningsMatchingRemember ➀Factual ➀
Cell Disruption Techniques from DescriptionsWOMCRemember ➀Factual ➀
DNA Sequencing Techniques from DescriptionsWOMCRemember ➀Factual ➀
Latin Phrases from Their MeaningsWOMCRemember ➀Factual ➀
Complementary DNA Sequences (5'/3' Labels)MCApply ➂Procedural ➂
Consensus Sequences from AlignmentsMCApply ➂Procedural ➂
DNA Fragment Migration in Agarose Gel Electrophoresis (Closest/Farthest)MCUnderstand ➁Conceptual ➁
DNA Fragment Size from Agarose Gel Migration DataMCApply ➂Procedural ➂
PCR Primer Pair Selection from Double-Stranded DNA (9 bp Template, 6-nt Primers)MCApply ➂Procedural ➂

Protein Biotechnology

NameQuestion typeBloom classification
Protein and Nucleic Acid Gel Electrophoresis ComponentsMatchingRemember ➀Factual ➀
Proteomics and Metabolomics Techniques from DescriptionsMatchingRemember ➀Conceptual ➁
Protein StabilityT/F Statements (MC)Remember ➀Conceptual ➁
Protein and Nucleic Acid Gel Electrophoresis ComponentsWOMCUnderstand ➁Factual ➀
Proteomics and Metabolomics Techniques from DescriptionsWOMCRemember ➀Conceptual ➁
Protein Net Charge at a Given pHMCApply ➂Conceptual ➁
Protein Molecular Weight from an SDS-PAGE LadderMCApply ➂Procedural ➂
Protein Molecular Weight from SDS-PAGE MigrationMCApply ➂Procedural ➂

Environmental and Synthetic Biology

NameQuestion typeBloom classification
Biofuel Sources and ChallengesMatchingRemember ➀Factual ➀
Culture Enrichment Methods from DescriptionsMatchingRemember ➀Factual ➀
Plant Genetic Modifications from FunctionsMatchingRemember ➀Factual ➀
Remediation Categories from SitesMatchingRemember ➀Factual ➀
Environmental 16S rDNA Survey Workflow (6 Steps)ORDAnalyze ➃Procedural ➂
Environmental 16S rDNA Survey Workflow (6 Steps)Sequence MCAnalyze ➃Procedural ➂
Glucose Measurement Through SkinT/F Statements (MC)Understand ➁Conceptual ➁
2026 Nobel Prize TopicsT/F Statements (MC)Remember ➀Factual ➀
Agrobacterium, Gene Guns, and CRISPRT/F Statements (MC)Remember ➀Factual ➀
xDNA and XNAT/F Statements (MC)Remember ➀Factual ➀
Biofuel Sources and ChallengesWOMCRemember ➀Factual ➀
Culture Enrichment Methods from DescriptionsWOMCRemember ➀Factual ➀
Plant Genetic Modifications from FunctionsWOMCRemember ➀Factual ➀
Sites by Remediation CategoryWOMCRemember ➀Factual ➀

Medicinal Biotechnology

NameQuestion typeBloom classification
Genetic Disease Treatment Methods from DescriptionsMatchingUnderstand ➁Conceptual ➁
Stem Cell Potency Levels from DefinitionsMatchingRemember ➀Conceptual ➁
Genetic Disease Treatment Methods from DescriptionsWOMCUnderstand ➁Conceptual ➁
Stem Cell Potency Levels from DefinitionsWOMCRemember ➀Conceptual ➁

Regulation in Biotechnology

NameQuestion typeBloom classification
Clinical Trial Phases from DescriptionsMatchingRemember ➀Conceptual ➁
Types of Intellectual Property from DescriptionsMatchingRemember ➀Conceptual ➁
Federal Agencies from Transgenic Crop Regulatory RolesMatchingRemember ➀Factual ➀
Patentable Inventions Versus Scientific DiscoveriesT/F Statements (MC)Understand ➁Conceptual ➁
Clinical Trial Phases from DescriptionsWOMCRemember ➀Conceptual ➁
Types of Intellectual Property from DescriptionsWOMCRemember ➀Conceptual ➁
Federal Agencies from Transgenic Crop Regulatory RolesWOMCRemember ➀Factual ➀

Bioethics in Biotechnology

NameQuestion typeBloom classification
Theranos Case Study: People and RolesMatchingRemember ➀Factual ➀
Theranos Case Study: People and RolesWOMCRemember ➀Factual ➀

Other

Cell Biology

NameQuestion typeBloom classification
Cell Receptors from DescriptionsMatchingRemember ➀Factual ➀
Cellular Structures from DescriptionsMatchingRemember ➀Factual ➀
Intercellular Junctions from FunctionsMatchingRemember ➀Factual ➀
Sensory Modalities from Receptor Cell TypesMatchingRemember ➀Factual ➀
Sensory Signal Transduction Components from FunctionsMatchingRemember ➀Factual ➀
Taste Qualities from StimuliMatchingRemember ➀Factual ➀
Stem Cell Potency Levels from DefinitionsMatchingRemember ➀Conceptual ➁
Trophic Categories from Metabolic DefinitionsMatchingRemember ➀Conceptual ➁
Trophic Classification from Energy and Carbon SourcesMatchingRemember ➀Conceptual ➁
Cell TheoryT/F Statements (MC)Remember ➀Conceptual ➁
Fluid Mosaic ModelT/F Statements (MC)Remember ➀Conceptual ➁
Membrane Permeability to Ions and MoleculesT/F Statements (MC)Understand ➁Conceptual ➁
Membrane Protein FunctionsT/F Statements (MC)Remember ➀Factual ➀
Mitosis and MeiosisT/F Statements (MC)Remember ➀Conceptual ➁
Second Messenger IdentificationT/F Statements (MC)Remember ➀Factual ➀
Chemosensation (Smell and Taste)T/F Statements (MC)Remember ➀Conceptual ➁
Sensory Coding (Smell vs. Taste)T/F Statements (MC)Remember ➀Conceptual ➁
Vision and Hearing TransductionT/F Statements (MC)Remember ➀Conceptual ➁
Cell Receptors from DescriptionsWOMCRemember ➀Conceptual ➁
Cellular Structures from DescriptionsWOMCRemember ➀Factual ➀
Intercellular Junctions from FunctionsWOMCRemember ➀Factual ➀
Sensory Modalities from Receptor Cell TypesWOMCRemember ➀Factual ➀
Sensory Signal Transduction Components from FunctionsWOMCRemember ➀Factual ➀
Taste Qualities from StimuliWOMCRemember ➀Factual ➀
Stem Cell Potency Levels from DefinitionsWOMCRemember ➀Conceptual ➁
Trophic Categories from Metabolic DefinitionsWOMCRemember ➀Conceptual ➁
Trophic Classification from Energy and Carbon SourcesWOMCRemember ➀Conceptual ➁
Cell Efficiency from Surface-Area-to-Volume RatioMCApply ➂Conceptual ➁

Biophysics

NameQuestion typeBloom classification
FRET Donor-Acceptor Pairs from Spectral OverlapMCApply ➂Conceptual ➁
FRET Color Assignments from Donor-Acceptor PermutationsMCApply ➂Conceptual ➁