7: RNA Transcription

Transcription of genes, RNA polymerase, promoters, and regulation.

Genes Expressed by Activator Proteins

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To promote gene expression, enhancers are specific DNA sequences that can be recognized and bound by transcription factors, including activator proteins.
Consider the following set of activator proteins:

FF   HH   RR   UU   XX   YY

Available Activator Proteins

Below are five different genes, each with its own set of enhancers.
Determine which of the genes are likely to be expressed based on the given set of activator proteins.
Note that more than one answer may be correct.

• A.

  HH

  RR

  UU

  FF

  promotor

  GENE 1

• B.

  RR

  YY

  FF

  XX

  promotor

  GENE 2

• C.

  MM

  YY

  PP

  promotor

  GENE 3

• D.

  YY

  XX

  HH

  RR

  UU

  promotor

  GENE 4

• E.

  PP

  YY

  MM

  promotor

  GENE 5

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mRNA Sequences from DNA Templates (Fill in the Blank)

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3′–

T

T

C

,

G

A

C

,

A

G

G

–5′

Enter the sequence for the mRNA product in the 5′ to 3′ direction produced from transcription of the DNA template strand above.
you may include a comma every 3 letters, but do NOT include any extra commas or spaces in your answer.
also do NOT include the 5' and 3' in your answer.

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mRNA Sequences from DNA Templates (5' and 3' Direction)

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5′–

A

T

G

,

G

A

A

,

C

G

C

–3′

Which one of the following sequences below would be the mRNA product produced from transcription of the DNA template strand above?
Hint: pay close attention to the 5′ and 3′ directions!

• A.  

  3′–

  G

  C

  G

  ,

  U

  U

  C

  ,

  C

  A

  U

  –5′

• B.  

  3′–

  C

  G

  C

  ,

  A

  A

  G

  ,

  G

  U

  A

  –5′

• C.  

  3′–

  U

  A

  C

  ,

  C

  U

  U

  ,

  G

  C

  G

  –5′

• D.  

  3′–

  A

  U

  G

  ,

  G

  U

  U

  ,

  G

  C

  G

  –5′

• E.  

  3′–

  A

  U

  G

  ,

  G

  A

  A

  ,

  C

  G

  C

  –5′

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mRNA Sequences from DNA Templates (5' and 3' Fill in the Blank)

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3′–

A

T

A

,

C

C

T

,

A

C

C

–5′

What is the transcribed RNA sequence to the DNA template strand sequence 3'-ATACCTACC-5' ?
you may include a comma every 3 letters, but do NOT include any extra commas or spaces in your answer.
Write your nucleotide sequence only in the 5′ -> 3′ direction

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