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11: PCR and Electrophoresis

Students extract DNA, perform initial and nested PCR amplification of the GAPDH gene, run gel electrophoresis to separate DNA fragments, and interpret band patterns.

Gene Expression Fold Change from RT-qPCR Data

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NUM Apply Procedural

DNA Fragment Migration in Agarose Gel Electrophoresis (Closest/Farthest)

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MC Understand Conceptual

DNA Fragment Size from Agarose Gel Migration Data

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NUM Apply Procedural

Inverse PCR Primer Selection (15 nt)

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MC Apply Procedural

Protein Molecular Weights from SDS-PAGE Band Positions

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Matching Remember Factual

Protein Molecular Weight from an SDS-PAGE Ladder

BBQ Text WeBWorK PGML
NUM Apply Procedural

Restriction Fragment Sizes from Linear DNA Maps (12 kb, 3 Sites, Isolated Fragment)

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MA Apply Procedural

Nested PCR Primer Pair Selection (24 nt)

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MC Apply Procedural

PCR Primer Pair Selection from Double-Stranded DNA (36 bp Template, 9-nt Primers)

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MC Apply Procedural

Protein Molecular Weight from SDS-PAGE Migration

BBQ Text WeBWorK PGML
NUM Apply Procedural