8: RNA Processing and CRISPR

RNA splicing, mRNA maturation, and CRISPR gene editing.

True/False Statements About Intron Removal Mechanisms

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Which one of the following statements is TRUE of intron removal mechanisms?

• A. both the group Ⅰ intron and the group Ⅱ intron do NOT require ATP energy input
• B. after splicing, the group Ⅱ intron forms a lariat structure with a guanine on the 5′ end
• C. group Ⅰ introns use a phosphoester transfer mechanism, while group Ⅱ introns do not
• D. the group Ⅱ intron uses an internal guanine nucleotide for catalytic activity
• E. self-splicing group Ⅰ introns and spliceosomal introns share a common mechanism

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True/False Statements About mRNA Post-Transcriptional Modification

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Which one of the following statements is TRUE regarding post-transcriptional mRNA modification?

• A. a modified adenosine is linked to the first nucleotide
• B. removal the intervening, non-coding sequences
• C. mRNA proofreading
• D. addition of a poly-guanidinylation tail on the 3′ end

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Alternative Splicing Possibilities

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5′ UTR	exon 1 100 bp	intron 2	exon 2 160 bp	intron 3	exon 3 120 bp	intron 4	exon 4 110 bp	3′ UTR
190 bp		170 bp		80 bp		70 bp		200 bp

The eukaryotic pre-mRNA shown above can be alternatively spliced in a number of different ways.

Which one of the following is NOT a possible processed mRNA?

• A.

  5′ cap	exon 1 100 bp	exon 3 120 bp	exon 4 110 bp	polyA tail
  80 bp				120 bp

  
  
• B.

  5′ cap	exon 3 120 bp	exon 2 160 bp	exon 4 110 bp	polyA tail
  80 bp				120 bp

  
  
• C.

  5′ cap	exon 1 100 bp	exon 2 160 bp	exon 3 120 bp	exon 4 110 bp	polyA tail
  80 bp					120 bp

  
  
• D.

  5′ cap	exon 1 100 bp	exon 2 160 bp	exon 3 120 bp	polyA tail
  80 bp				120 bp

  
  
• E.

  5′ cap	exon 1 100 bp	exon 2 160 bp	exon 4 110 bp	polyA tail
  80 bp				120 bp

  
  

Check Answer