8: RNA Processing and CRISPR

RNA splicing, mRNA maturation, and CRISPR gene editing.

True/False Statements About Intron Removal Mechanisms

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Which one of the following statements is TRUE regarding intron removal mechanisms?

• A. after splicing, the group Ⅱ intron forms a lariat structure with an adenine on the 5′ end
• B. group Ⅱ introns are never self-splicing
• C. spliceosomal introns and self-splicing group Ⅰ introns share a common mechanism
• D. the group Ⅱ intron uses an exogenous guanine nucleoside for catalytic activity
• E. group Ⅰ introns use a phosphoester transfer mechanism, while group Ⅱ introns do not

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True/False Statements About mRNA Post-Transcriptional Modification

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Which one of the following statements is TRUE regarding post-transcriptional mRNA modification?

• A. addition of a poly-guanidinylation tail on the 3′ end
• B. removal the intervening, non-coding sequences
• C. reverse transcription of the mRNA
• D. a modified adenosine is placed on the 5′ end

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Alternative Splicing Possibilities

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5′ UTR	exon 1 160 bp	intron 2	exon 2 130 bp	intron 3	exon 3 180 bp	intron 4	exon 4 140 bp	3′ UTR
110 bp		70 bp		120 bp		190 bp		90 bp

The eukaryotic pre-mRNA shown above can be alternatively spliced in a number of different ways.

Which one of the following is NOT a possible processed mRNA?

• A.

  5′ cap	exon 1 160 bp	exon 3 180 bp	exon 4 140 bp	polyA tail
  80 bp				120 bp

  
  
• B.

  5′ cap	exon 2 130 bp	exon 1 160 bp	exon 3 180 bp	polyA tail
  80 bp				120 bp

  
  
• C.

  5′ cap	exon 2 130 bp	exon 3 180 bp	exon 4 140 bp	polyA tail
  80 bp				120 bp

  
  
• D.

  5′ cap	exon 1 160 bp	exon 2 130 bp	exon 4 140 bp	polyA tail
  80 bp				120 bp

  
  
• E.

  5′ cap	exon 1 160 bp	exon 2 130 bp	exon 3 180 bp	exon 4 140 bp	polyA tail
  80 bp					120 bp

  
  

Check Answer