2: Biophysics

Students analyze physical methods applied to biological molecules, including FRET energy transfer, fluorescence spectroscopy, binding curves, diffusion, membrane potentials, and single-molecule techniques.

FRET Donor-Acceptor Pairs from Spectral Overlap

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Förster resonance energy transfer (FRET) requires both an acceptor fluorescent protein and a donor fluorescent protein. Which one of the following color combinations would be an effective FRET setup?

A. The donor protein absorbs violet (410 nm) and emits green (530 nm); the acceptor protein absorbs violet (410 nm) and emits orange (600 nm).
B. The donor protein absorbs orange (600 nm) and emits green (530 nm); the acceptor protein absorbs orange (600 nm) and emits violet (410 nm).
C. The donor protein absorbs violet (410 nm) and emits green (530 nm); the acceptor protein absorbs orange (600 nm) and emits green (530 nm).
D. The donor protein absorbs violet (410 nm) and emits green (530 nm); the acceptor protein absorbs green (530 nm) and emits orange (600 nm).
E. The donor protein absorbs orange (600 nm) and emits green (530 nm); the acceptor protein absorbs violet (410 nm) and emits green (530 nm).

FRET Color Assignments from Donor-Acceptor Permutations

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Förster resonance energy transfer (FRET) requires both an acceptor fluorescent protein and a donor fluorescent protein. Which one of the following color combinations would be an effective FRET setup?

A. The donor protein absorbs red (700 nm) and emits blue (460 nm); the acceptor protein absorbs blue (460 nm) and emits violet (410 nm).
B. The donor protein absorbs violet (410 nm) and emits blue (460 nm); the acceptor protein absorbs blue (460 nm) and emits red (700 nm).
C. The donor protein absorbs blue (460 nm) and emits violet (410 nm); the acceptor protein absorbs violet (410 nm) and emits red (700 nm).
D. The donor protein absorbs violet (410 nm) and emits red (700 nm); the acceptor protein absorbs red (700 nm) and emits blue (460 nm).
E. The donor protein absorbs red (700 nm) and emits violet (410 nm); the acceptor protein absorbs violet (410 nm) and emits blue (460 nm).
F. The donor protein absorbs blue (460 nm) and emits red (700 nm); the acceptor protein absorbs red (700 nm) and emits violet (410 nm).