2: Biophysics

Students analyze physical methods applied to biological molecules, including FRET energy transfer, fluorescence spectroscopy, binding curves, diffusion, membrane potentials, and single-molecule techniques.

FRET Color Combinations

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Förster resonance energy transfer (FRET) requires both an acceptor fluorescent protein and a donor fluorescent protein. Which one of the following color combinations would be an effective FRET setup?

• A. The donor protein absorbs red (700 nm) and emits yellow (580 nm); the acceptor protein absorbs yellow (580 nm) and emits blue (460 nm).
• B. The donor protein absorbs red (700 nm) and emits yellow (580 nm); the acceptor protein absorbs blue (460 nm) and emits yellow (580 nm).
• C. The donor protein absorbs red (700 nm) and emits yellow (580 nm); the acceptor protein absorbs red (700 nm) and emits blue (460 nm).
• D. The donor protein absorbs blue (460 nm) and emits yellow (580 nm); the acceptor protein absorbs yellow (580 nm) and emits red (700 nm).
• E. The donor protein absorbs blue (460 nm) and emits yellow (580 nm); the acceptor protein absorbs blue (460 nm) and emits red (700 nm).

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FRET Color Combinations

Blackboard Learn TXT Blackboard Ultra QTI v2.1 Canvas/ADAPT QTI v1.2 Human-Readable TXT

Click to show FRET Color Combinations example problem

Förster resonance energy transfer (FRET) requires both an acceptor fluorescent protein and a donor fluorescent protein. Which one of the following color combinations would be an effective FRET setup?

• A. The donor protein absorbs indigo (430 nm) and emits orange (600 nm); the acceptor protein absorbs orange (600 nm) and emits green (530 nm).
• B. The donor protein absorbs indigo (430 nm) and emits green (530 nm); the acceptor protein absorbs green (530 nm) and emits orange (600 nm).
• C. The donor protein absorbs orange (600 nm) and emits green (530 nm); the acceptor protein absorbs green (530 nm) and emits indigo (430 nm).
• D. The donor protein absorbs green (530 nm) and emits indigo (430 nm); the acceptor protein absorbs indigo (430 nm) and emits orange (600 nm).
• E. The donor protein absorbs green (530 nm) and emits orange (600 nm); the acceptor protein absorbs orange (600 nm) and emits indigo (430 nm).
• F. The donor protein absorbs orange (600 nm) and emits indigo (430 nm); the acceptor protein absorbs indigo (430 nm) and emits green (530 nm).

Check Answer