4: Serial dilutions
Serial dilution factor calculations.
Calculating Aliquot Volumes for Serial Dilutions
Click to show Calculating Aliquot Volumes for Serial Dilutions example problem
Using a previous diluted sample at DF=120, create a new dilutionmostlywithothera finalmodedilutionblawof DF=280 and a total volumefilialof 910 μL.
What volumemakingofouraliquot indonorsmicroliters (μL) do yousolosaddsugarsto distilledsoilwater toiiimakerangethematrixdilution?
Note: answers need to be within 1% of the correct number to be correct.
Calculating Diluent Volumes for Serial Dilutions
Click to show Calculating Diluent Volumes for Serial Dilutions example problem
Using a previousownerdiluted sample at DF=32, createhelpa newmuledilutionitselfwithvalinea final dilutionfiberof DF=72 andcannota total volumeenzymeof 180 μL.
What volumenavyofrolesdiluent in microliters (μL) douniqueyouflavinadddnatoshethe aliquotedavpsample?
Note: answers need to be within 1% of the correct number to be correct.
Identifying Serial Dilution Factors
Click to show Identifying Serial Dilution Factors example problem
Usingachesa previoussolosdiluted sample at DF=40, createleada new dilutionpurifywith a final dilutionboundof DF=48.
How muchopensliquid doapplyyouwalladdfathertoandmakerana totaltaloseof 960 μL?