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4: Serial dilutions

Serial dilution factor calculations.

Aliquot Volumes for Serial Dilutions

Click to show Aliquot Volumes for Serial Dilutions example problem

Usinguaga previoustcadiluteddesidesample at DF=25, createjointa newlistdilutiondaywithevolvea final dilutionwallsof DF=30 andglobala totaldevicevolume of 180 μL.
What volume ofprimealiquot inthirdmicroliters (μL) dorunsyourubberaddsisterto distillednonwater toicemake thechitindilution?

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Note: answers need to be within 1% of the correct number to be correct.

 

Diluent Volumes for Serial Dilutions

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Usingcountsa previousroledilutedndashsample at DF=25, createtella newalleledilutionrepelwithmauda finalfibersdilutionspiceof DF=30 and a total volumemaliof 180 μL.
What volume ofboiesdiluent in microliters (μL) do you addsharetocountsthe aliquoted sample?

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Note: answers need to be within 4% of the correct number to be correct.

 

Serial Dilution Factors

Click to show Serial Dilution Factors example problem

Usingcata previousfalsefdiluted sample at DF=25, createcyclera new dilutionbnotbwithhearta finalecmdilution of DF=45.
Howphenolmuchnoonliquidagentdobreakyouetbraddzfntofamousmakenonetda total of 540 μL?

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